Patents by Inventor Rolf Nagel
Rolf Nagel has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220112537Abstract: The present disclosure deals with the biochemistry of reagents useful in the detection of ammonia in liquid samples. Specifically, the present disclosure is directed to a technical improvement of an enzyme-based test for ammonia that can be used for analysis of plasma samples taken from patients in clinical settings, among other uses. In this regard, stability of a reagent containing NAD(P)H is improved, enhancing shelf life and results in the detection of ammonia. In an exemplary reagent ammonia released as a result of NAD(P)H decay is scavenged using an enzymatic reaction to convert the ammonia using GLDH, NAD(P)H and 2-oxoglutarate, thereby forming L-glutamate, NAD(P)+ and H2O in the NAD(P)H containing reagent.Type: ApplicationFiled: January 30, 2020Publication date: April 14, 2022Inventors: Steffen BOSSERT-REUTHER, Rolf NAGEL, Silvia RETTIG
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Publication number: 20170073661Abstract: Methods are provided for stabilizing an enzyme by storing the enzyme in the presence of a stabilized coenzyme. In addition, enzymes are provided that are stabilized with a stabilized coenzyme, as well as the use thereof in test elements for detecting analytes. Other aspects include unique compositions, methods, techniques, systems and devices involving enzyme stabilization.Type: ApplicationFiled: November 29, 2016Publication date: March 16, 2017Inventors: Wolfgang Roedel, Carina Horn, Nelli Steinke, Nadine Bucci, Thomas Meier, Rainer Schmuck, Rolf Nagel, Dieter Heindl
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Patent number: 9540702Abstract: Methods for stabilizing an enzyme by storing the enzyme in the presence of a stabilized coenzyme are disclosed. In addition, an enzyme stabilized with a stabilized coenzyme as well as the use thereof in test elements for detecting analytes are also disclosed. Other aspects include unique compositions, methods, techniques, systems and devices involving enzyme stabilization.Type: GrantFiled: February 20, 2012Date of Patent: January 10, 2017Assignee: Roche Diabetes Care, Inc.Inventors: Wolfgang Roedel, Carina Horn, Nelli Steinke, Nadine Bucci, Thomas Meier, Rainer Schmuck, Rolf Nagel, Dieter Heindl
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Publication number: 20120276565Abstract: Methods for stabilizing an enzyme by storing the enzyme in the presence of a stabilized coenzyme are disclosed. In addition, an enzyme stabilized with a stabilized coenzyme as well as the use thereof in test elements for detecting analytes are also disclosed. Other aspects include unique compositions, methods, techniques, systems and devices involving enzyme stabilization.Type: ApplicationFiled: February 20, 2012Publication date: November 1, 2012Inventors: Wolfgang Roedel, Carina Horn, Nelli Steinke, Nadine Bucci, Thomas Meier, Rainer Schmuck, Rolf Nagel, Dieter Heindl
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Patent number: 7410103Abstract: Embodiments of the present invention disclose an efficient and inexpensive method for detecting tampered products utilizing current off the shelf components. Features of some embodiments provide for a RFID labeling system that uses multiple RFID components attached to a single product unit being embedded in the product's label and one or more tamper labels. Such RFID labels usually comprise an adhesive label containing an RFID component, a destructible conducting path that acts as an antenna and a unique encryption. The RFID labels transmit the encryption when irradiated by an RF scanner. A complete inventory of multiple associated encryptions reported during an RFID scan of a product package provide positive indication that product has not been tampered with. A missing encryption indicates a faulty product seal or an event of tampering.Type: GrantFiled: March 13, 2006Date of Patent: August 12, 2008Inventor: Andreas Rolf Nagel
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Publication number: 20070178546Abstract: The present invention relates to the use of a polar organic solvent for the stabilization of a cholinesterase substrate solution, wherein at least one substrate is stabilized by at least one species of a polar organic solvent in a buffered solution, and to the use of said solution for determining the activity of cholinesterase in a sample. Also within the scope of the present invention is a stabilized cholinesterase substrate solution for determining the activity of a cholinesterase in a sample, wherein at least one substrate is stabilized by at least one species of a polar organic solvent. Furthermore, the invention relates to a method for determining the activity of a cholinesterase in a sample, as well as a kit for determining the activity of a cholinesterase in a sample and a kit for conducting a method for determining the activity of a cholinesterase in a sample.Type: ApplicationFiled: December 5, 2006Publication date: August 2, 2007Inventors: Friederike Weber, Norbert Gottschalk, Hans-Joachim Kytzia, Matthias Muecke, Rolf Nagel
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Patent number: 7078480Abstract: The invention concerns a method for stabilizing the content of glycated protein in a sample on a matrix material, which is characterized in that the matrix material is impregnated with boric acid buffer with a pH which is larger or equal to 10.5 or a transition metal salt as well as an appropriate matrix material, an element for collecting, transporting and storing sample material to be analysed with such a matrix material and a system containing such an element and a sealable covering.Type: GrantFiled: March 28, 2003Date of Patent: July 18, 2006Assignee: Roche Diagnostics GmbHInventors: Rolf Nagel, Jürgen Mistele
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Patent number: 6680120Abstract: A baked enamel with a thermoplastic polyamide, wherein the the enamel comprises a cross-linking agent that causes chemical cross-linking of the thermoplastic polymer under the action of heat, whereby the cross-linking agent comprises a polyvalent alcohol, a polyisocyanate as well as polyol. A wire with a topcoat of such a baked enamel is also described.Type: GrantFiled: December 7, 1999Date of Patent: January 20, 2004Assignee: NexansInventors: Rolf Nagel, Gerhard Boockmann, Laurent Preux
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Publication number: 20030199079Abstract: The invention concerns a method for stabilizing the content of glycated protein in a sample on a matrix material, which is characterized in that the matrix material is impregnated with boric acid buffer with a pH which is larger or equal to 10.5 or a transition metal salt as well as an appropriate matrix material, an element for collecting, transporting and storing sample material to be analysed with such a matrix material and a system containing such an element and a sealable covering.Type: ApplicationFiled: March 28, 2003Publication date: October 23, 2003Inventors: Rolf Nagel, Jurgen Mistele
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Patent number: 6552165Abstract: The invention concerns a method for stabilizing the content of glycated protein in a sample on a matrix material, which is characterized in that the matrix material is impregnated with boric acid buffer with a pH which is larger or equal to 10.5 or a transition metal salt as well as an appropriate matrix material, an element for collecting, transporting and storing sample material to be analyzed with such a matrix material and a system containing such an element and a sealable covering.Type: GrantFiled: May 21, 1999Date of Patent: April 22, 2003Assignee: Roche Diagnostics GmbHInventors: Rolf Nagel, Jürgen Mistele
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Patent number: 6413733Abstract: A stabilized reagent for the photometric enzymatic determination of creatine kinase (CK) in biological sample material by forming ATP from creatine phosphate and ADP and detecting the ATP formed, containing an organic or inorganic sulphur compound preferably in a submolar amount relative to the added CK activator. A corresponding reagent in a liquid form is stable at 2 to 8° C. for up to 12 months without significant loss of function.Type: GrantFiled: June 9, 2000Date of Patent: July 2, 2002Assignee: Roche Diagnostics GmbHInventors: Rolf Nagel, Juergen Mistele, Norbert Schroeder
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Patent number: 6231815Abstract: The invention concerns a system for the storage and transport of sample material on absorbent material which is characterized in that the system contains no test reagents and it additionally includes a closable container containing a medium that can absorb moisture such as a desiccant in addition to the absorbent material for absorbing a liquid sample. Furthermore the system according to the invention can contain an agent to stabilize the sample material and optionally further auxiliary substances.Type: GrantFiled: November 20, 1997Date of Patent: May 15, 2001Assignee: Roche Diagnostics GmbHInventors: Gregor Bainczyk, Rolf Nagel, Helmut Leininger, Rolf Lerch
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Patent number: 6087592Abstract: An enameled wire having high resistance to partial discharges includes an electrical conductor and at least two layers of enamel on the conductor. At least one of these layers is made from resin charged with weakly conductive particles. The outermost layer constitutes an overcoat and is made from thermo-adherent resin. At least the layer directly underlying the overcoat is made from the resin charged with the particles.Type: GrantFiled: February 24, 1998Date of Patent: July 11, 2000Assignee: AlcatelInventors: Rolf Nagel, Virginie Studer, Laurent Preux, Leonard Danel
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Patent number: 6071553Abstract: In a method for the production of melt-bonding wires to enable the use of non-solvent-containing thermoplastic or thermosetting coating materials, meltable thermoplastic or thermosetting materials are supplied to a fusion chamber (8) where they are melted. The insulated electrical conductor (1) to be coated is drawn through the melt and after the coating process leaves the coating chamber (12) through a calibration device (5), which is followed by cooling.Type: GrantFiled: July 28, 1997Date of Patent: June 6, 2000Assignee: AlcatelInventors: Maurice Ferne, Rolf Nagel, Pierre Yves Le Tiec
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Patent number: 5959076Abstract: The invention concerns a method for stabilizing the content of glycated protein in a sample on a matrix material, which is characterized in that the matrix material is impregnated with boric acid buffer with a pH which is larger or equal to 10.5 or a transition metal salt as well as an appropriate matrix material, an element for collecting, transporting and storing sample material to be analysed with such a matrix material and a system containing such an element and a sealable covering.Type: GrantFiled: August 26, 1996Date of Patent: September 28, 1999Assignee: Roche Diagnostics GmbHInventors: Rolf Nagel, Jurgen Mistele
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Patent number: 5948631Abstract: The subject matter of the invention is a stable mixture for the detection of alkaline phosphatase, wherein it contains the N-methylglucamine salt of o-cresolphthalein monophosphoric acid and N-methylglucamine as well as an analytical element for the detection of alkaline phosphatase with a matrix that carries this mixture. In addition the invention concerns a method for the determination of alkaline phosphatase using a salt of o-cresolphthalein monophosphoric acid, wherein the sample is brought into contact with a mixture according to the invention or an analytical element according to the invention and a change in color is observed in the presence of alkaline phosphatase.A subject matter of the invention is also a salt of o-cresolphthalein monophosphoric acid with N-methylglucamine as well as a process for producing such a salt.Type: GrantFiled: August 7, 1997Date of Patent: September 7, 1999Assignee: Roche Diagnostics GmbHInventors: Rolf Nagel, Steffen Bossert-Reuther, Thomas Zeibig
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Patent number: 5374546Abstract: The invention concerns a process for stabilizing the enzyme 1-methylhydantoinase (NMHase) which is characterized in that the NMHase is treated with divalent metal ions, a nucleoside triphosphate and a complexing agent which complexes these divalent metal ions. In addition the invention concerns the use of an enzyme stabilized in this way in a method for the determination of an analyte in which 1-methylhydantoin is converted, as well as a corresponding reagent.Type: GrantFiled: January 16, 1992Date of Patent: December 20, 1994Assignee: Boehringer Mannheim GmbHInventors: Rolf Nagel, Ulfert Deneke, Jurgen Mistele
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Patent number: 5274095Abstract: Diarylimidazoles of the general formula: ##STR1## wherein R.sup.1 is a radical of the general formula: ##STR2## in which R.sup.3 and R.sup.6 are hydrogen atoms, R.sup.4 and R.sup.5, which can be the same or different, are lower alkyl radicals or R.sup.3 /R.sup.4 and /or R.sup.5 /R.sup.6 in each case together represent a lower alkylene radical and R.sup.2 is a lower alkyl radical, and/or of a salt thereof, are useful as redox indicators, particularly in the presence of a SH group-containing substance.Type: GrantFiled: August 11, 1992Date of Patent: December 28, 1993Assignee: Boehringer Mannheim GmbHInventors: Hans-Peter Braun, Ulfert Deneke, Werner Guethlein, Rolf Nagel
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Patent number: 5162200Abstract: Diarylimidazoles of the general formula: ##STR1## wherein R.sup.1 is a radical of the general formula: ##STR2## in which R.sup.3 and R.sup.6 are hydrogen atoms, R.sup.4 and R.sup.5, which can be the same or different, are lower alkyl radicals or R.sup.3 /R.sup.4 and/or R.sup.5 /R.sup.6 in each case together represent a lower alkylene radical and R.sup.2 is a lower alkyl radical, and/or of a salt thereof, are used as redox indicators in the colorimetric diarylimidazoles are not influenced by the presence of a SH group-containing substance. The peroxidate-acting substance can be used to determine the presence of creatine kinase.Type: GrantFiled: May 30, 1990Date of Patent: November 10, 1992Assignee: Boehringer Manneheim GmbHInventors: Hans-Peter Braun, Ulfert Deneke, Werner Guethlein, Rolf Nagel
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Patent number: 4876067Abstract: The present invention provides a multi-layer test carrier (1) for the analytical determination of a component in a liquid sample, especially in a body fluid, having a first carrier layer (2) and a second carrier layer (10), at least one of which is transparent, a liquid-absorbing layer (3) being applied to the first carrier layer (2) and a reagent layer (11) containing at least one reagent being applied to the second carrier layer (10), the carrier layers being so arranged and fixed that the surfaces (3a, 11a) of the absorbing layer and of the reagent layer facing away from the carrier layer can be brought into contact with one another and can be pressed against one another, wherein the reagent layer (11) is soluble substantially without residue in the sample liquid and the take-up ability of the liquid-absorbing layer (3) for the sample liquid is so great that an amount of liquid absorbed therein suffices in order to dissolve the reagent layer free of residues so that substances contained in the reagent layeType: GrantFiled: August 25, 1987Date of Patent: October 24, 1989Assignee: Boehringer Mannheim GmbHInventors: Ulfert Deneke, Rolf Nagel, Anselm Rothe, Helmut Freitag