Patents by Inventor Rolf Nagel

Rolf Nagel has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20220112537
    Abstract: The present disclosure deals with the biochemistry of reagents useful in the detection of ammonia in liquid samples. Specifically, the present disclosure is directed to a technical improvement of an enzyme-based test for ammonia that can be used for analysis of plasma samples taken from patients in clinical settings, among other uses. In this regard, stability of a reagent containing NAD(P)H is improved, enhancing shelf life and results in the detection of ammonia. In an exemplary reagent ammonia released as a result of NAD(P)H decay is scavenged using an enzymatic reaction to convert the ammonia using GLDH, NAD(P)H and 2-oxoglutarate, thereby forming L-glutamate, NAD(P)+ and H2O in the NAD(P)H containing reagent.
    Type: Application
    Filed: January 30, 2020
    Publication date: April 14, 2022
    Inventors: Steffen BOSSERT-REUTHER, Rolf NAGEL, Silvia RETTIG
  • Publication number: 20170073661
    Abstract: Methods are provided for stabilizing an enzyme by storing the enzyme in the presence of a stabilized coenzyme. In addition, enzymes are provided that are stabilized with a stabilized coenzyme, as well as the use thereof in test elements for detecting analytes. Other aspects include unique compositions, methods, techniques, systems and devices involving enzyme stabilization.
    Type: Application
    Filed: November 29, 2016
    Publication date: March 16, 2017
    Inventors: Wolfgang Roedel, Carina Horn, Nelli Steinke, Nadine Bucci, Thomas Meier, Rainer Schmuck, Rolf Nagel, Dieter Heindl
  • Patent number: 9540702
    Abstract: Methods for stabilizing an enzyme by storing the enzyme in the presence of a stabilized coenzyme are disclosed. In addition, an enzyme stabilized with a stabilized coenzyme as well as the use thereof in test elements for detecting analytes are also disclosed. Other aspects include unique compositions, methods, techniques, systems and devices involving enzyme stabilization.
    Type: Grant
    Filed: February 20, 2012
    Date of Patent: January 10, 2017
    Assignee: Roche Diabetes Care, Inc.
    Inventors: Wolfgang Roedel, Carina Horn, Nelli Steinke, Nadine Bucci, Thomas Meier, Rainer Schmuck, Rolf Nagel, Dieter Heindl
  • Publication number: 20120276565
    Abstract: Methods for stabilizing an enzyme by storing the enzyme in the presence of a stabilized coenzyme are disclosed. In addition, an enzyme stabilized with a stabilized coenzyme as well as the use thereof in test elements for detecting analytes are also disclosed. Other aspects include unique compositions, methods, techniques, systems and devices involving enzyme stabilization.
    Type: Application
    Filed: February 20, 2012
    Publication date: November 1, 2012
    Inventors: Wolfgang Roedel, Carina Horn, Nelli Steinke, Nadine Bucci, Thomas Meier, Rainer Schmuck, Rolf Nagel, Dieter Heindl
  • Patent number: 7410103
    Abstract: Embodiments of the present invention disclose an efficient and inexpensive method for detecting tampered products utilizing current off the shelf components. Features of some embodiments provide for a RFID labeling system that uses multiple RFID components attached to a single product unit being embedded in the product's label and one or more tamper labels. Such RFID labels usually comprise an adhesive label containing an RFID component, a destructible conducting path that acts as an antenna and a unique encryption. The RFID labels transmit the encryption when irradiated by an RF scanner. A complete inventory of multiple associated encryptions reported during an RFID scan of a product package provide positive indication that product has not been tampered with. A missing encryption indicates a faulty product seal or an event of tampering.
    Type: Grant
    Filed: March 13, 2006
    Date of Patent: August 12, 2008
    Inventor: Andreas Rolf Nagel
  • Publication number: 20070178546
    Abstract: The present invention relates to the use of a polar organic solvent for the stabilization of a cholinesterase substrate solution, wherein at least one substrate is stabilized by at least one species of a polar organic solvent in a buffered solution, and to the use of said solution for determining the activity of cholinesterase in a sample. Also within the scope of the present invention is a stabilized cholinesterase substrate solution for determining the activity of a cholinesterase in a sample, wherein at least one substrate is stabilized by at least one species of a polar organic solvent. Furthermore, the invention relates to a method for determining the activity of a cholinesterase in a sample, as well as a kit for determining the activity of a cholinesterase in a sample and a kit for conducting a method for determining the activity of a cholinesterase in a sample.
    Type: Application
    Filed: December 5, 2006
    Publication date: August 2, 2007
    Inventors: Friederike Weber, Norbert Gottschalk, Hans-Joachim Kytzia, Matthias Muecke, Rolf Nagel
  • Patent number: 7078480
    Abstract: The invention concerns a method for stabilizing the content of glycated protein in a sample on a matrix material, which is characterized in that the matrix material is impregnated with boric acid buffer with a pH which is larger or equal to 10.5 or a transition metal salt as well as an appropriate matrix material, an element for collecting, transporting and storing sample material to be analysed with such a matrix material and a system containing such an element and a sealable covering.
    Type: Grant
    Filed: March 28, 2003
    Date of Patent: July 18, 2006
    Assignee: Roche Diagnostics GmbH
    Inventors: Rolf Nagel, Jürgen Mistele
  • Patent number: 6680120
    Abstract: A baked enamel with a thermoplastic polyamide, wherein the the enamel comprises a cross-linking agent that causes chemical cross-linking of the thermoplastic polymer under the action of heat, whereby the cross-linking agent comprises a polyvalent alcohol, a polyisocyanate as well as polyol. A wire with a topcoat of such a baked enamel is also described.
    Type: Grant
    Filed: December 7, 1999
    Date of Patent: January 20, 2004
    Assignee: Nexans
    Inventors: Rolf Nagel, Gerhard Boockmann, Laurent Preux
  • Publication number: 20030199079
    Abstract: The invention concerns a method for stabilizing the content of glycated protein in a sample on a matrix material, which is characterized in that the matrix material is impregnated with boric acid buffer with a pH which is larger or equal to 10.5 or a transition metal salt as well as an appropriate matrix material, an element for collecting, transporting and storing sample material to be analysed with such a matrix material and a system containing such an element and a sealable covering.
    Type: Application
    Filed: March 28, 2003
    Publication date: October 23, 2003
    Inventors: Rolf Nagel, Jurgen Mistele
  • Patent number: 6552165
    Abstract: The invention concerns a method for stabilizing the content of glycated protein in a sample on a matrix material, which is characterized in that the matrix material is impregnated with boric acid buffer with a pH which is larger or equal to 10.5 or a transition metal salt as well as an appropriate matrix material, an element for collecting, transporting and storing sample material to be analyzed with such a matrix material and a system containing such an element and a sealable covering.
    Type: Grant
    Filed: May 21, 1999
    Date of Patent: April 22, 2003
    Assignee: Roche Diagnostics GmbH
    Inventors: Rolf Nagel, Jürgen Mistele
  • Patent number: 6413733
    Abstract: A stabilized reagent for the photometric enzymatic determination of creatine kinase (CK) in biological sample material by forming ATP from creatine phosphate and ADP and detecting the ATP formed, containing an organic or inorganic sulphur compound preferably in a submolar amount relative to the added CK activator. A corresponding reagent in a liquid form is stable at 2 to 8° C. for up to 12 months without significant loss of function.
    Type: Grant
    Filed: June 9, 2000
    Date of Patent: July 2, 2002
    Assignee: Roche Diagnostics GmbH
    Inventors: Rolf Nagel, Juergen Mistele, Norbert Schroeder
  • Patent number: 6231815
    Abstract: The invention concerns a system for the storage and transport of sample material on absorbent material which is characterized in that the system contains no test reagents and it additionally includes a closable container containing a medium that can absorb moisture such as a desiccant in addition to the absorbent material for absorbing a liquid sample. Furthermore the system according to the invention can contain an agent to stabilize the sample material and optionally further auxiliary substances.
    Type: Grant
    Filed: November 20, 1997
    Date of Patent: May 15, 2001
    Assignee: Roche Diagnostics GmbH
    Inventors: Gregor Bainczyk, Rolf Nagel, Helmut Leininger, Rolf Lerch
  • Patent number: 6087592
    Abstract: An enameled wire having high resistance to partial discharges includes an electrical conductor and at least two layers of enamel on the conductor. At least one of these layers is made from resin charged with weakly conductive particles. The outermost layer constitutes an overcoat and is made from thermo-adherent resin. At least the layer directly underlying the overcoat is made from the resin charged with the particles.
    Type: Grant
    Filed: February 24, 1998
    Date of Patent: July 11, 2000
    Assignee: Alcatel
    Inventors: Rolf Nagel, Virginie Studer, Laurent Preux, Leonard Danel
  • Patent number: 6071553
    Abstract: In a method for the production of melt-bonding wires to enable the use of non-solvent-containing thermoplastic or thermosetting coating materials, meltable thermoplastic or thermosetting materials are supplied to a fusion chamber (8) where they are melted. The insulated electrical conductor (1) to be coated is drawn through the melt and after the coating process leaves the coating chamber (12) through a calibration device (5), which is followed by cooling.
    Type: Grant
    Filed: July 28, 1997
    Date of Patent: June 6, 2000
    Assignee: Alcatel
    Inventors: Maurice Ferne, Rolf Nagel, Pierre Yves Le Tiec
  • Patent number: 5959076
    Abstract: The invention concerns a method for stabilizing the content of glycated protein in a sample on a matrix material, which is characterized in that the matrix material is impregnated with boric acid buffer with a pH which is larger or equal to 10.5 or a transition metal salt as well as an appropriate matrix material, an element for collecting, transporting and storing sample material to be analysed with such a matrix material and a system containing such an element and a sealable covering.
    Type: Grant
    Filed: August 26, 1996
    Date of Patent: September 28, 1999
    Assignee: Roche Diagnostics GmbH
    Inventors: Rolf Nagel, Jurgen Mistele
  • Patent number: 5948631
    Abstract: The subject matter of the invention is a stable mixture for the detection of alkaline phosphatase, wherein it contains the N-methylglucamine salt of o-cresolphthalein monophosphoric acid and N-methylglucamine as well as an analytical element for the detection of alkaline phosphatase with a matrix that carries this mixture. In addition the invention concerns a method for the determination of alkaline phosphatase using a salt of o-cresolphthalein monophosphoric acid, wherein the sample is brought into contact with a mixture according to the invention or an analytical element according to the invention and a change in color is observed in the presence of alkaline phosphatase.A subject matter of the invention is also a salt of o-cresolphthalein monophosphoric acid with N-methylglucamine as well as a process for producing such a salt.
    Type: Grant
    Filed: August 7, 1997
    Date of Patent: September 7, 1999
    Assignee: Roche Diagnostics GmbH
    Inventors: Rolf Nagel, Steffen Bossert-Reuther, Thomas Zeibig
  • Patent number: 5374546
    Abstract: The invention concerns a process for stabilizing the enzyme 1-methylhydantoinase (NMHase) which is characterized in that the NMHase is treated with divalent metal ions, a nucleoside triphosphate and a complexing agent which complexes these divalent metal ions. In addition the invention concerns the use of an enzyme stabilized in this way in a method for the determination of an analyte in which 1-methylhydantoin is converted, as well as a corresponding reagent.
    Type: Grant
    Filed: January 16, 1992
    Date of Patent: December 20, 1994
    Assignee: Boehringer Mannheim GmbH
    Inventors: Rolf Nagel, Ulfert Deneke, Jurgen Mistele
  • Patent number: 5274095
    Abstract: Diarylimidazoles of the general formula: ##STR1## wherein R.sup.1 is a radical of the general formula: ##STR2## in which R.sup.3 and R.sup.6 are hydrogen atoms, R.sup.4 and R.sup.5, which can be the same or different, are lower alkyl radicals or R.sup.3 /R.sup.4 and /or R.sup.5 /R.sup.6 in each case together represent a lower alkylene radical and R.sup.2 is a lower alkyl radical, and/or of a salt thereof, are useful as redox indicators, particularly in the presence of a SH group-containing substance.
    Type: Grant
    Filed: August 11, 1992
    Date of Patent: December 28, 1993
    Assignee: Boehringer Mannheim GmbH
    Inventors: Hans-Peter Braun, Ulfert Deneke, Werner Guethlein, Rolf Nagel
  • Patent number: 5162200
    Abstract: Diarylimidazoles of the general formula: ##STR1## wherein R.sup.1 is a radical of the general formula: ##STR2## in which R.sup.3 and R.sup.6 are hydrogen atoms, R.sup.4 and R.sup.5, which can be the same or different, are lower alkyl radicals or R.sup.3 /R.sup.4 and/or R.sup.5 /R.sup.6 in each case together represent a lower alkylene radical and R.sup.2 is a lower alkyl radical, and/or of a salt thereof, are used as redox indicators in the colorimetric diarylimidazoles are not influenced by the presence of a SH group-containing substance. The peroxidate-acting substance can be used to determine the presence of creatine kinase.
    Type: Grant
    Filed: May 30, 1990
    Date of Patent: November 10, 1992
    Assignee: Boehringer Manneheim GmbH
    Inventors: Hans-Peter Braun, Ulfert Deneke, Werner Guethlein, Rolf Nagel
  • Patent number: 4876067
    Abstract: The present invention provides a multi-layer test carrier (1) for the analytical determination of a component in a liquid sample, especially in a body fluid, having a first carrier layer (2) and a second carrier layer (10), at least one of which is transparent, a liquid-absorbing layer (3) being applied to the first carrier layer (2) and a reagent layer (11) containing at least one reagent being applied to the second carrier layer (10), the carrier layers being so arranged and fixed that the surfaces (3a, 11a) of the absorbing layer and of the reagent layer facing away from the carrier layer can be brought into contact with one another and can be pressed against one another, wherein the reagent layer (11) is soluble substantially without residue in the sample liquid and the take-up ability of the liquid-absorbing layer (3) for the sample liquid is so great that an amount of liquid absorbed therein suffices in order to dissolve the reagent layer free of residues so that substances contained in the reagent laye
    Type: Grant
    Filed: August 25, 1987
    Date of Patent: October 24, 1989
    Assignee: Boehringer Mannheim GmbH
    Inventors: Ulfert Deneke, Rolf Nagel, Anselm Rothe, Helmut Freitag