Abstract: A method of detecting and/or discriminating mismatched or complementary nucleic acids using a field-effect transistor (FET). The FET comprises source and drain electrodes formed on substrate and separated by a channel that includes a thin semiconducting film. One or more nucleic acid molecules are immobilized to thin semiconducting film. The FET includes a gate electrode in contact with solution containing the sample (or located on the surface of the device). Samples possibly containing target nucleic acid are exposed to the FET of the biosensor device and current response is measured after samples containing target nucleic acid have been exposed to the FET of the biosensor device. Measured current response in the FET is used to detect and/or discriminate whether target nucleic acid is present as well as complementary or mismatched. Measured current response may also be used to differentiate among different mismatches.

Abstract: Methods for inhibiting proliferation of a B-cell population are provided comprising administering a combination of agents, comprising: (i) at least one P13K-delta selective inhibitor; (ii) at least one anti-CD20 antibody; and (iii) at least one Bruton's tyrosine kinase (BTK) inhibitor. Methods for treating B-cell proliferative disorders, such as B-cell hematological malignancies, as well as kits for carrying out the claimed methods, are also provided.

Abstract: The present invention relates to biomimetic membrane compositions and methods for making and using them.

Abstract: A dental hydrogel composition comprising: (a) polysaccharide; (b) polydopamine conjugated to the polysaccharide, wherein between 5 and 35 percent of polysaccharide sugar moieties are conjugated to polydopamine; (c) RGD peptide coupled to the polysaccharide-poly-dopamine conjugate; and (d) moieties that are crosslinkable upon exposure to light coupled to the polysaccharide; wherein components (a)-(d) are disposed in the composition such that the hydrogel composition: exhibits an adhesive strength of at least 10 kPa upon cross linking of crosslinkable moieties; and exhibits an elasticity between 5 kPa and 100 kPa upon cross linking of crosslinkable moieties.

Abstract: This disclosure relates to Cas9-nucleic acid complexes and uses related thereto. In certain embodiments, the disclosure contemplates transgenic plants and animals genetically engineered to express Cas9-nucleic acid complexes disclosed herein. In certain embodiments, the disclosure relates to methods of treating or preventing, diseases, conditions, cancer, viral infections or other pathogenic infection using vectors configured to express a Cas9-nucleic acid complex disclosed herein.

Abstract: A method of transporting biomolecular cargo intracellularly into cells includes the operations of providing magnetic nanostructures (e.g., nanospears, nanostars, nanorods, and other nanometer-sized structures) carrying the biomolecular cargo thereon and applying an external magnetic field to move the magnetic nanostructures into physical contact with at least some of the cells (or the cells into the magnetic nanostructures). The magnetic nanostructures move into physical contact with a single cell, a subset of cells, or all cells. The external magnetic field may be applied by a moving permanent magnet although an electromagnetic may also be used. The biomolecular cargo may include a molecule, a plurality of molecules, or higher order biological constructs.

Abstract: Highly effective combinations of a compound of formula A (a PI3K? selective inhibitor) and anti-CD20 antibodies are provided herein for the treatment and amelioration of PI3K? and/or CD20 mediated diseases and disorders. In particular, the combination can be used to treat cancers and autoimmune diseases. More particularly, the invention provides for a combination of a compound of formula A, or stereoisomers thereof, and ublituximab for the treatment and/or amerioration of hematological malignancies such as leukemia and lymphoma. The invention also provides for a combination of a compound of formula A, or stereoisomers thereof, and anti-CD20 antibodies, including ublituximab for the treatment and/or amelioration of multiple scelrosis.

Abstract: A device includes an optical fiber bundle having at least one optical data fiber and at least three optical tracking fibers, a mirror package configured to direct an incoming optical beam to the optical fiber bundle, at least three detectors, each detector corresponding to one of the at least three optical tracking fibers, the at least three detectors configured to receive portions of the incoming optical beam from the corresponding optical tracking fibers and convert the portions of the incoming beam to electrical tracking signals, and a controller configured to receive the electrical tracking signals from the at least three detectors and generate a feedback control based on the electrical tracking signals to control a position of the mirror package.

Abstract: Highly effective combinations of a compound of formula A (a PI3K? selective inhibitor) and anti-CD20 antibodies are provided herein for the treatment and amelioration of PI3K? and/or CD20 mediated diseases and disorderes. In particular, the combination can be used to treat cancers and autoimmune diseases. More particularly, the invention provided for a combination of a compound of formula A, or stereoisomers thereof, and ublituximab for the treatment and/or amerioration of hematological malignancies such as leukemia and lymphoma.

Abstract: A device includes an optical fiber bundle having at least one optical data fiber and at least three optical tracking fibers, a mirror package configured to direct an incoming optical beam to the optical fiber bundle, at least three detectors, each detector corresponding to one of the at least three optical tracking fibers, the at least three detectors configured to receive portions of the incoming optical beam from the corresponding optical tracking fibers and convert the portions of the incoming beam to electrical tracking signals, and a controller configured to receive the electrical tracking signals from the at least three detectors and generate a feedback control based on the electrical tracking signals to control a position of the mirror package.

Abstract: Systems and methods of fabricating and functionality patterned nanowire probes that are stable under fluid reservoir conditions and have imageable contrast are provided. Optical imaging and deconstruction methods and systems are also provided that are capable of determining the distribution of nanowires of a particular pattern to determine the mixing between or leakage from fluid reservoirs.

Abstract: A robust and general fabrication/manufacturing method is described herein for the fabrication of periodic three-dimensional (3D) hierarchical nanostructures in a highly scalable and tunable manner. This nanofabrication technique exploits the selected and repeated etching of spherical particles that serve as resist material and that can be shaped in parallel for each processing step. The method enables the fabrication of periodic, vertically aligned nanotubes at the wafer scale with nanometer-scale control in three dimensions including outer/inner diameters, heights/hole-depths, and pitches. The method was utilized to construct 3D periodic hierarchical hybrid silicon and hybrid nanostructures such as multi-level solid/hollow nanotowers where the height and diameter of each level of each structure can be configured precisely as well as 3D concentric plasmonic supported metal nanodisk/nanorings with tunable optical properties on a variety of substrates.

Abstract: This disclosure relates to Cas9-nucleic acid complexes and uses related thereto. In certain embodiments, the disclosure contemplates transgenic plants and animals genetically engineered to express Cas9-nucleic acid complexes disclosed herein. In certain embodiments, the disclosure relates to methods of treating or preventing, diseases, conditions, cancer, viral infections or other pathogenic infection using vectors configured to express a Cas9-nucleic acid complex disclosed herein.

Abstract: Techniques are described controlling and managing machines to provide laundry services. A request may be received from a mobile device of a user, the request indicating that the mobile device is in proximity to a laundry machine such as a washer or dryer. In some cases, the request is sent by the mobile device based on detecting, at the mobile device, a wireless signal transmitted from the laundry machine. In some cases the request may include a code that identifies the laundry machine, the code being displayed by the machine and/or encoded in a scannable code displayed by the machine. In response to the request, a signal may be sent to activate the laundry machine and enable the user to use the laundry machine for a predetermined period of time (e.g., a cycle). In some cases, the activation signal is sent if the user's payment is successfully processed.

Abstract: A microfluidic-based device and system is disclosed for the high-throughput intracellular delivery of biomolecular cargo to cells (eukaryotic or prokaryotic) or enveloped viruses. Cargo integration occurs due to transient membrane permeabilization by exposure to bulk acoustic waves (BAWs) transduced from surface acoustic waves (SAWs) generated by a rapidly oscillating piezoelectric substrate. In this approach, temporary pores are established across the cellular membrane as cells are partially deformed and squeezed or subject to shearing forces as they travel through the vibrational modes created within the microfludic channel(s) of the device.

Abstract: Techniques are described controlling and managing machines to provide laundry services. A request may be received from a mobile device of a user, the request indicating that the mobile device is in proximity to a laundry machine such as a washer or dryer. In some cases, the request is sent by the mobile device based on detecting, at the mobile device, a wireless signal transmitted from the laundry machine. In some cases the request may include a code that identifies the laundry machine, the code being displayed by the machine and/or encoded in a scannable code displayed by the machine. In response to the request, a signal may be sent to activate the laundry machine and enable the user to use the laundry machine for a predetermined period of time (e.g., a cycle). In some cases, the activation signal is sent if the user's payment is successfully processed.

Abstract: The present invention relates to biomimetic membrane compositions and methods for making and using them.

Abstract: The disclosure relates to the expansion and differentiation of mesenchymal stem cells and bone marrow cells, including retention of stem cell plasticity during expansion and differentiation of stem cells to produce osteocytes, chondrocytes and other cells of the mesodermal lineage.

Abstract: A dispenser having a lid with an opening for dispensing individual flexible sheets wherein the flexible sheets are formed from a continuous sheet of material having perforations at predetermined intervals. The lid having a centrally located annular groove and concentric raised portion surrounding an opening disposed in the lid to permit the continuous sheet material to pass therethrough, and a resilient gasket having an opening corresponding in size to the opening in the lid. The gasket having a peripheral edge attached to the lid with the opening of the gasket aligned with the opening in the lid.

Abstract: The present disclosure provides methods and kits for treating or slowing the progression of a hematological malignancy, by administering to a subject in need thereof a therapeutically effective amount of: (i) at least one inhibitor of PI3 kinase (PI3K)-delta (e.g., TGR-1202); (ii) at least one anti-CD20 antibody (e.g., ublituximab); and (iii) at least one anti-PD-1 antibody (e.g., pembrolizumab) or anti-PD-Ll antibody (e.g., atezolizumab). Treatment regimens are also provided.