Abstract: The present invention relates to a photobioreactor, and more particularly, a photobioreactor for culturing living organisms such as microalgae, which carry out photosynthesis using carbon dioxide and light energy. The photobioreactor includes: (a) a reaction vessel, in which photosynthesis occurs by photosynthetic organisms; (b) a multipurpose inlet/outlet formed at the outside upper end of the reaction vessel; (c) an outer pipe connected to the multipurpose inlet/outlet at the outside of the reaction vessel; and (d) an inner pipe connected to the multipurpose inlet/outlet at the inside of the reaction vessel, wherein the reaction vessel is made of a transparent film. The photobioreactor according to the present invention is advantageous in that the reaction vessel in which photosynthesis occurs is a plate-type and made of a transparent film, thus achieving improved light transmittance and mobility, and enabling the economically advantageous manufacture and operation thereof.

Abstract: The present invention relates to microalgae with improved phototaxis and photosynthetic efficiency, and more particularly, to a microalgae PTS42 (KCTC18499P) mutant of Chlamydomonas reinhardtii and uses thereof. In the microalgae PTS42 according to the present invention of which photosensitivity and photosynthetic efficiency are improved due to excellent phototaxis, conversion of carbon dioxide into biomass is excellent as compared to a wild type strain and a maximum photosynthesis rate and lipid accumulation ability are also high, such that the microalgae PTS42 are useful as a photosynthetic strain for constructing a biofuel production process as well as an effect of decreasing carbon dioxide.

Abstract: The present invention relates to a method for preparing metal nanostructures using DNA, and more particularly, to a method for preparing metal nanostructures, in which a self-assembling DNA is used as a frame, and thus the orientation, shape and size of the nanostructures are easily controlled compared to conventional bottom-up methods. Metal nanostructures prepared by the method show excellent localized surface plasmon resonance properties, and thus can be used as fluorescent substances in drug delivery, biomedical imaging, and supersensitive biosensors.

Abstract: The present invention relates to a drug delivery vehicle comprising a polydiacetylene liposome, wherein a lipid bilayer is formed by a mixture of 10,12-pentacosadiynoic acid (PCDA), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), and N-palmitoyl homocysteine (PHC), and a drug to be delivered is encapsulated in an isolated inner space of a polydiacetylene liposome. The PDA liposome drug delivery vehicle of the present invention comprises a lipid layer formed by mixing different phospholipids, excluding polydiacetylene, so as to be stable, and thus any leakage problems of an encapsulated drug are eliminated. In addition, since the liposome is sensitive to a pH, the shape and size thereof can be readily changed through the formation of a liposome-liposome conjugate by the improved sensitivity of the drug delivery vehicle under a specific acidic requirement, thereby enabling selective drug release, and thus can be applied as a drug delivery vehicle for various target materials.

Abstract: The present invention discloses a microfluidic photoreaction system and a method for screening a single cell organism having changed light-response characteristics. According to the present invention, an improved single cell organism can be effectively screened based on phototaxis using a microfluidic system. Specifically, easy monitoring at the cellular level is possible, and a mutant strain having an increased response and/or sensitivity to light can be easily and rapidly screened by various analyses, including statistical analysis of collected results. Thus, the present invention can be effectively used to investigate the correlation between phototaxis and photoconversion efficiency and to screen a single cell organism having increased photosynthetic efficiency.

Abstract: A method for screening an anticancer candidate is disclosed. The method includes immobilizing gold nanoparticles onto a substrate; binding a protein involved in carcinogenesis and metastasis to the immobilized gold nanoparticles, recording a spectrum of the protein conjugate, and analyzing the spectrum to obtain reference data; adding a candidate inhibiting the activity of the protein to the protein conjugate, recording a spectrum of the mixture, and analyzing the spectrum to obtain comparative data; and comparing the reference data with the comparative data to determine whether the candidate inhibits the activity of the protein. The method enables screening of a candidate inhibiting the activity of a protein involved in carcinogenesis and metastasis in an accurate and convenient manner.

Abstract: The present invention relates to a method for preparing metal nanostructures using DNA, and more particularly, to a method for preparing metal nanostructures, in which a self-assembling DNA is used as a frame, and thus the orientation, shape and size of the nanostructures are easily controlled compared to conventional bottom-up methods. Metal nanostructures prepared by the method show excellent localized surface plasmon resonance properties, and thus can be used as fluorescent substances in drug delivery, biomedical imaging, supersensitive biosensors, etc.

Abstract: The present disclosure relates to a strain capable of producing succinate using starch accumulated in microalgae which grow using carbon dioxide as a direct carbon source without converting it to glucose and a method for producing succinate using the same. The present disclosure provides a strain producing succinate from carbon dioxide, selected from a group consisting of Corynebacterium glutamicum BL-1-pBlAmyS (KCTC 12585BP) and Corynebacterium glutamicum BL-1-pSbAmyA (KCTC 12587BP). The present disclosure also provides a method for producing succinate from carbon dioxide, including fermenting starch by inoculating the strain producing succinate from carbon dioxide in a starch-containing medium.

Abstract: The present invention discloses a microfluidic photoreaction system and a method for screening a single cell organism having changed light-response characteristics. According to the present invention, an improved single cell organism can be effectively screened based on phototaxis using a microfluidic system. Specifically, easy monitoring at the cellular level is possible, and a mutant strain having an increased response and/or sensitivity to light can be easily and rapidly screened by various analyses, including statistical analysis of collected results. Thus, the present invention can be effectively used to investigate the correlation between phototaxis and photoconversion efficiency and to screen a single cell organism having increased photosynthetic efficiency.

Abstract: The present invention relates to a drug delivery vehicle comprising a polydiacetylene liposome, wherein lipid bilayer is formed by a mixture of 10,12-pentacosadiynoic acid (PCDA), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), and N-palmitoyl homocysteine (PHC), and a drug to be delivered is encapsulated in an isolated inner space of a polydiacetylene liposome. The PDA liposome drug delivery vehicle of the present invention comprises a lipid layer formed by mixing different phospholipids, excluding polydiacetylene, so as to be stable, and thus any leakage problems of an encapsulated drug are eliminated. In addition, since the liposome is sensitive to a pH, the shape and size thereof can be readily changed through the formation of a liposome-liposome conjugate by the improved sensitivity of the drug delivery vehicle under a specific acidic requirement, thereby enabling selective drug release, and thus can be applied as a drug delivery vehicle for various target materials.

Abstract: The present invention relates to a photobioreactor, and more particularly, a photobioreactor for culturing living organisms such as microalgae, which carry out photosynthesis using carbon dioxide and light energy. The photobioreactor includes: (a) a reaction vessel, in which photosynthesis occurs by photosynthetic organisms; (b) a multipurpose inlet/outlet formed at the outside upper end of the reaction vessel; (c) an outer pipe connected to the multipurpose inlet/outlet at the outside of the reaction vessel; and (d) an inner pipe connected to the multipurpose inlet/outlet at the inside of the reaction vessel, wherein the reaction vessel is made of a transparent film. The photobioreactor according to the present invention is advantageous in that the reaction vessel in which photosynthesis occurs is a plate-type and made of a transparent film, thus achieving improved light transmittance and mobility, and enabling the economically advantageous manufacture and operation thereof.

Abstract: Disclosed is a method of detecting even a very small amount of a target substance by mixing a linker and a spacer at a suitable ratio and immobilizing the mixture on the surface of carbon nanotubes in a carbon nanotube-based biosensor. This method detects a specific substance at the level of femtomoles and lowers the detection limit of conventional carbon nanotube transistor sensors. Accordingly, the method detects even a very small amount of a target substance, and thus the carbon nanotube-based biosensor is a highly useful sensor which can be used either as a medical sensor for diagnosing diseases or as an environmental sensor.

Abstract: Provided are a method for detecting biomaterials, a method for fabricating a chip for biomaterial detection and a chip for biomaterial detection. The method for detecting biomaterials is characterized by comprising the steps of: (S1) immobilizing polydiacetylene liposomes onto a substrate; (S2) linking the polydiacetylene liposomes together and layering them on the substrate; (S3) immobilizing a material which forms a complementary binding with a subject biomaterial to be detected onto the polydiacetylene liposomes; (S4) exposing the resulted polydiacetylene liposome to UV light so as to form a chip for biomaterial detection; (S5) applying the subject biomaterial to be detected to the chip for biomaterial detection for reaction; and (S6) measuring a fluorescent signal from the chip for biomaterial detection.

Abstract: Disclosed is a method of detecting bioproducts using Localized Surface Plasmon Resonance (LSPR) of gold nanoparticles, which can diagnose bioproducts based on changes in the maximum wavelength occurred by an antigen-antibody reaction after immobilization of the gold nanoparticles onto a glass panel. A sensor using such method exhibits high sensitivity, is low in price, and makes quick diagnosis possible, thereby being applicable to various biological fields associated with environmental contaminants, pathogens and the like, as well as diagnosis of diseases. Further, it provides a technology for manufacturing a sensor having higher sensitivity, low price and quick performance, as compared to conventional methods using SPR.

Abstract: Disclosed is a method of pretreating lignocellulose-based biomass by extracting lignin from biomass by adding a solvent for dissolving lignin to the lignocellulose-based biomass including lignin, hemicellulose and cellulose, and extracting the cellulose and/or hemicellulose by adding an ionic liquid to the remaining biomass after extracting the lignin.

Abstract: There is provided a method of separating free-astaxanthin selectively from green algae and, more particularly, to a method of separating free-astaxanthin selectively from Haematococcus pluvialis, the method comprising: mixing a cell culture containing Haematococcus pluvialis with an alkanic solvent and stirring, thereby obtaining an alkanic solvent extract containing astaxanthin material including free-astaxanthin and astaxanthin ester (step 1); and mixing the alkanic solvent extract with an alcohol and stirring, thereby obtaining an alcoholic extract containing free-astaxanthin (step 2).

Abstract: There is provided a polydiacetylene supramolecule comprising diacetylene molecules, capable of immobilizing a receptor molecule having a thiol group. Since the polydiacetylene supramolecule has a receptor immobilized thereon having a thiol group, for example, an antibody, and thus shows color transition when reacting with a sample, an antigen can be detected through specific color transition of the polydiacetylene when employing in a receptor-ligand reaction, for example, an antibody-antigen reaction.

Abstract: There is provided a method of detecting a pathogenic microorganism in real-time, using a modified flow-type surface plasmon resonance (SPR) biosensor, comprising the steps of: i) performing, in a batch-type, an immune reaction of a pathogenic microorganism and an antibody thereto; ii) selectively separating the pathogenic microorganism bound with the antibody; and iii) binding the pathogenic microorganism bound with the antibody on a surface of a chip of a flow-type SPR sensor system in real-time.

Abstract: Provided are a method for detecting biomaterials, a method for fabricating a chip for biomaterial detection and a chip for biomaterial detection. The method for detecting biomaterials is characterized by comprising the steps of: (S1) immobilizing polydiacetylene liposomes onto a substrate; (S2) linking the polydiacetylene liposomes together and layering them on the substrate; (S3) immobilizing a material which forms a complementary binding with a subject biomaterial to be detected onto the polydiacetylene liposomes; (S4) exposing the resulted polydiacetylene liposome to UV light so as to form a chip for biomaterial detection; (S5) applying the subject biomaterial to be detected to the chip for biomaterial detection for reaction; and (S6) measuring a fluorescent signal from the chip for biomaterial detection.

Abstract: The present invention relates to a method that improves productivity of a useful secondary metabolite by eliminating biological by-products effectively during the culture of a useful secondary metabolite-producing microorganism, and more particularly, in order to efficiently eliminate biological by-products formed during the culture of Sorangium cellulosum, the present invention relates to a method for producing epothilones comprising that a cation exchange resin is added to said culture medium or said culture broth is recirculated through a column charged with a cation exchange resin.