Abstract: The disclosed technology relates to a method of analyzing DNA from a biological sample. In one aspect, the method comprises obtaining a sample a sample from a subject, the sample comprising DNA that comprises double-stranded DNA (dsDNA) suspected of having one or more thymine lesions formed by deamination of 5-methylcytosines, wherein the thymine lesions form base pair mismatches in the dsDNA, contacting the sample with a nuclease that digests dsDNA at base pair mismatches; harvesting DNA from the nuclease-treated sample; and performing an analysis on undigested DNA harvested from the nuclease-treated sample.

Abstract: Provided herein is technology relating to detecting neoplasia and particularly, but not exclusively, to methods, compositions, and related uses for detecting neoplasms such as lung cancer.

Abstract: The disclosed technology relates to a method of analyzing DNA from a biological sample. In one aspect, the method comprises obtaining a sample a sample from a subject, the sample comprising DNA that comprises double-stranded DNA (dsDNA) suspected of having one or more thymine lesions formed by deamination of 5-methylcytosines, wherein the thymine lesions form base pair mismatches in the dsDNA, contacting the sample with a nuclease that digests dsDNA at base pair mismatches; harvesting DNA from the nuclease-treated sample; and performing an analysis on undigested DNA harvested from the nuclease-treated sample.

Abstract: The disclosed technology relates to a method of analyzing a tissue sample. In one aspect, the method comprises obtaining a formalin-fixed paraffin-embedded (FFPE) tissue sample; contacting the tissue sample with Mung Bean Nuclease to cleave mismatched DNA pairs when isolating DNA from the tissue sample; and performing an analysis on the non-digested DNA. In one embodiment, the tissue sample is obtained from a patient having a refractory disease or a cancer during biopsy, and the analysis comprises performing next-generation DNA sequencing to evaluate genomic DNA mutations. In another embodiment, the method further comprises using a computer to screen for one or more therapies according to the cancer diagnosis; and producing a computer-generated report of possible therapies.

Abstract: A method includes receiving information associated with health care services provided to a patient; determining for respective ones of the health care services respective probabilities that a first entity will refuse responsibility; associating a first portion of the health care services with the first entity; associating a second portion of the health care services with a second entity, the second portion of the health care services comprising respective ones of the health care services having probabilities that exceed a defined threshold; assigning responsibility for the first portion of the health care services to the first entity; and assigning responsibility for the second portion of the health care services to the second entity.

Abstract: The disclosed technology relates to a method of analyzing a tissue sample. In one aspect, the method comprises obtaining a formalin-fixed paraffin-embedded (FFPE) tissue sample; contacting the tissue sample with Mung Bean Nuclease to cleave mismatched DNA pairs when isolating DNA from the tissue sample; and performing an analysis on the non-digested DNA. In one embodiment, the tissue sample is obtained from a patient having a refractory disease or a cancer during biopsy, and the analysis comprises performing next-generation DNA sequencing to evaluate genomic DNA mutations. In another embodiment, the method further comprises using a computer to screen for one or more therapies according to the cancer diagnosis; and producing a computer-generated report of possible therapies.

Abstract: Systems and methods are disclosed that overcome problems with conventional filters for high temperature casting materials. According to embodiments, uniform, engineered filters enable consistent and repeatable metal flow rates without becoming a secondary source of contamination. Proven Additive Manufacturing (AM) methods generate engineered filters that achieve consistent filtration efficiency and predictable metal flow into a casting mold. Through this application of AM for ceramics, the resulting filter incorporates features that can be adjusted on-demand to address the needs of specific alloys and geometries applied in the production of castings. According to an embodiment, the method includes generating, using an additive manufacturing apparatus, a plurality of layers, wherein each layer includes individual ceramic ligaments arranged in a grid pattern having a two-dimensional rotational orientation. The method further includes stacking the layers along a thickness direction to form the filter.

Abstract: A system includes wireless access points operable to communicate with a communication network and electronic tracking devices that each include an electronic-ink display. The electronic tracking devices are operable to communicate wirelessly with the wireless access points. The system also includes manufacturing system operator computer systems operable to communicate with the communication network. The system further includes a server operable to execute instructions to update at least one entry in a manufacturing system database based on receiving an operation notification from one of the manufacturing system operator computer systems. The server can transmit a display update for the electronic-ink display of an identified electronic tracking device through at least one of the wireless access points based on determining that the operation notification includes a transition from a current manufacturing process step to a next manufacturing process step.

Abstract: A system includes wireless access points operable to communicate with a communication network and electronic tracking devices that each include an electronic-ink display. The electronic tracking devices are operable to communicate wirelessly with the wireless access points. The system also includes manufacturing system operator computer systems operable to communicate with the communication network. The system further includes a server operable to execute instructions to update at least one entry in a manufacturing system database based on receiving an operation notification from one of the manufacturing system operator computer systems. The server can transmit a display update for the electronic-ink display of an identified electronic tracking device through at least one of the wireless access points based on determining that the operation notification includes a transition from a current manufacturing process step to a next manufacturing process step.

Abstract: A system that incorporates teachings of the present disclosure may include, for example, a digital video recording (DVR) system having a controller to record video content supplied by an interactive television (iTV) communication system as directed by a plurality of users of the iTV communication system, receive from a communication device of one of the plurality of users a request for a portion of the recorded video content, identify a need to transcode the requested portion of the recorded video content according to one or more operating characteristics of the communication device, transcode the requested portion of the recorded video content to conform with the one or more operating characteristics of the communication device, and transmit to the communication device the transcoded video content. The DVR system can be located outside of the premises of each of the plurality of users. Other embodiments are disclosed.

Abstract: A disclosed system includes an additive manufacturing printer that performs a layer by layer three-dimensional printing process generating a casting mold based on a three-dimensional numerical specification. The numerical specification is based on a desired casting shape, including internal features such as hollow areas formed by cores, and is further based on a thermo-mechanical model of a casting process. The numerical specification describes variations in material and geometric properties of one or more layers of the casting mold corresponding to variations in the thermal and mechanical properties of the casting processes, as predicted by the thermo-mechanical model. The system may vary the thickness of features of the casting mold, based on predicted cooling rates, to reduce cooling non-uniformities and to provide for controlled, predictable cooling of the casting. The system may further generate trusses and heat sinks in the mold to respectively strengthen and weaken various features of the mold.

Abstract: Analyzing peripheral blood RNA populations presents an effective, accurate, minimally invasive method of determining a patient's cancer status. Using circulating free RNA of the genes disclosed herein, systems and methods are disclosed which can accurately identify cancer signatures in the patient blood samples.

Abstract: Analyzing peripheral blood RNA populations presents an effective, accurate, minimally invasive method of determining a patient's cancer status. Using circulating free RNA of the genes disclosed herein, systems and methods are disclosed which can accurately identify cancer signatures in the patient blood samples.

Abstract: A method includes receiving input data from user devices having access to media content of a media stream that is responsive to at least a subset of the input data. The input data may correspond to one or more user inputs received from the user devices via an interactive session associated with the media content. The method further includes selecting a particular subset of the input data based at least in part on one or more inputs received from a device. The one or more inputs may be responsive to selection of one or more input controls of a moderator control graphical user interface. The method further includes sending an adapted media stream to the user devices. The adapted media stream may include the media content and content corresponding to the particular subset of the input data.

Abstract: A method includes creating, at a media server, a media stream that includes a group of segments of media content associated with a user-selected category. The group of segments includes a first segment corresponding to a portion of a first program and a second segment corresponding to a portion of a second program. The method also includes sending at least a portion of the media stream to a user device. The method further includes, during playback of the media stream, receiving a user input indicating the first segment, and sending a modified stream to the user device based on the user input. The first segment is replaced by an entirety of the first program in the modified stream.

Abstract: Systems and methods of media stream generation based on categories of user expression are disclosed. In an embodiment, a method includes receiving input selecting at least one category from a plurality of categories of expression. Each of the plurality of categories is descriptive of a type of expression made by users as media content is presented. The method includes selecting a group of segments of the media content. Each of the segments of media content in the group is associated with the selected category. The method further includes generating a stream of media content that includes each of the segments of media content.

Abstract: Resetting a password for a network service account may include redirecting the user to a password reset tool, wherein the user is blocked from network access other than the password reset tool while being redirected. After redirecting the user to the password reset tool, user entry of verification information may be accepted, and the verification information from the user may be compared with known verification information for the user. User entry of a new password may be accepted if the verification information accepted from the user matches the known verification information for the user; and the new password may be stored as the known password for the user. Related systems and computer-program products are also discussed.

Abstract: Systems, devices, and methods for removing areas of tissue are described. A programmable laser may remove precise areas of tissue while the tissue remains substantially frozen. The laser is programmed in part by analyzing a reference image of a representative tissue section. A software program may receive digital images of test slices. Areas of interest in the image may be selected by a user. The software program can then create and send cut instructions to the programmable laser. The laser may be configured to make perforated cuts to remove the area of interest without melting the removed section.

Abstract: A method of displaying available multimedia content items includes receiving a request to display a set of available content items. In response to the request, a first set of icons are provided for display, where each icon corresponds to a type of available multimedia content. The icons are displayed in an approximately circular arrangement, providing for easy viewing and manipulation. In response to selection of an icon, a second set of icons is displayed in an approximately circular arrangement, where each icon of the second set corresponds to a sub-type of the available multimedia content. The user can continue to select icons based on the type of desired multimedia content until a list of available multimedia content items are displayed. Accordingly, the user is able to quickly locate and view multimedia content of a desired type, resulting in an improved user experience.

Abstract: Systems, devices, and methods for removing areas of tissue are described. A programmable laser may remove precise areas of tissue while the tissue remains substantially frozen. The laser is programmed in part by analyzing a reference image of a representative tissue section. A software program may receive digital images of test slices. Areas of interest in the image may be selected by a user. The software program can then create and send cut instructions to the programmable laser. The laser may be configured to make perforated cuts to remove the area of interest without melting the removed section.