Abstract: A fluid sample receptacle provides a means of assuring uncontaminated, multiple samples of a fluid specimen. An upper or isolated sample chamber and a lower or primary sample chamber provide redundant test samples. A cover or lid with a downwardly extending column includes a stopper with a removable plug attached to automatically seal the lower sample chamber when the cover is sealed across the top of the upper chamber. This feature guarantees that the sample chambers are sealed or isolated simultaneously and that the test samples are therefore identical when taken. Tamper evident seals prove that either sample has not been contaminated. A preferred embodiment provides a bellows assembly to accommodate variations in ambient pressure and to provide a positive means of expelling a precise quantity of fluid from the receptacle for testing.

Abstract: A method of obtaining aliquots or samples from a liquid specimen collection container and an apparatus which automateally perforths those methods involves transferring a sample of liquid from a sealed non-deformingly expandable container containing gas and the liquid without contaminating the liquid remaining inside the container, and comprises (a) non-deformingly volumetrically expanding the sealed container sufficiently to reduce pressure therein below ambient pressure outside the container, (b) applying heat selectively to a locus on said enlarged container to non-invasively create a hole having a diameter effective to equilibrate the pressure inside the container with said ambient pressure without loss of liquid from the container, and (c) non-deformingly volumetrically reducing the container to expel liquid from the container through the hole.