Patents by Inventor Seiichi Hara
Seiichi Hara has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20110104334Abstract: A fermented seasoning which does not have the bitterness specific to peptides and can be easily ingested orally, in spite of containing large amount of peptides is provided. Namely, a fermented seasoning obtained by adding salt water to an Aspergillus culture or a mixture of the Aspergillus culture with a plant-derived protein material and by controlling the leucine aminopeptidase-I activity in the moromi to be 1.0 U/ml or less, and also controlling the leucine aminopeptidase-II activity to be 0.5 U/ml or less, within 10 days after the preparation.Type: ApplicationFiled: July 2, 2009Publication date: May 5, 2011Applicant: KIKKOMAN CORPORATIONInventors: Takeharu Nakahara, Riichiro Uchida, Yoichi Hanada, Yoshikazu Endo, Shigeki Tanizawa, Hitomi Aota, Seiichi Hara
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Publication number: 20100279343Abstract: A method of producing an ?-L-aspartyl-L-phenylalanine-?-ester by forming the ?-L-aspartyl-L-phenylalanine-?-ester from L-aspartic acid-??-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to selectively link L-phenylalanine to an ?-ester site of the L-aspartic acid-??-diester through a peptide bond.Type: ApplicationFiled: March 30, 2010Publication date: November 4, 2010Applicant: AJINOMOTO CO., INC.Inventors: KENZO YOKOZEKI, AYAKO OHNO, SEIICHI HARA, ISAO ABE
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Publication number: 20100267082Abstract: DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.Type: ApplicationFiled: March 23, 2010Publication date: October 21, 2010Applicant: Ajinomoto Co., Inc.Inventors: Seiichi Hara, Kenzo Yokozeki, Isao Abe, Naoto Tonouchi, Yasuko Jojima
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Publication number: 20100221795Abstract: It is an object of the present invention to provide a procedure for realizing inexpensive and simple production of 3-indole-pyruvic acid. A transformant is made using a polynucleotide having a specific nucleotide sequence encoding a protein having an oxidase activity, and oxidase is generated by culturing the transformant in a medium to accumulate the oxidase in the medium and/or the transformant. Further, tryptophan is converted into 3-indole-pyruvic acid in the presence of the transformant and/or a culture thereof to produce 3-indole-pyruvic acid.Type: ApplicationFiled: February 24, 2010Publication date: September 2, 2010Applicant: AJINOMOTO CO. INCInventors: YASUAKI TAKAKURA, SEIICHI HARA, TOSHIKI TABA, SHUNICHI SUZUKI, MASAKAZU SUGIYAMA, KUNIHIKO WATANABE, KENZO YOKOZEKI
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Patent number: 7749742Abstract: A method is disclosed that allows the production of peptides having three or more amino acid residues easily, inexpensively and at high yield without going through a complex synthesis method. A novel enzyme that efficiently produces a peptide from bacteria belonging to the genus Empedobacter or the genus Sphingobacterium is provided. The enzyme acts on a carboxy component and an amine component to form peptides having three or more amino acid residues by acting on a carboxy component and an amine component.Type: GrantFiled: November 15, 2007Date of Patent: July 6, 2010Assignee: Ajinomoto Co., Inc.Inventors: Kenzo Yokozeki, Sonoko Suzuki, Seiichi Hara, Isao Abe
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Patent number: 7745172Abstract: A method for producing an ?-L-aspartyl-L-phenylalanine-?-ester (also named ?-L-(?-o-substituted aspartyl)-L-phenylalanine), which is an intermediate of an ?-L-aspartyl-L-phenylalanine-?-methyl ester (also named ?-L-aspartyl-L-phenylalanine methyl ester; product name: aspartame), easily, at high yield and inexpensively without going through a complex synthesis method is provided. Further, an easy, inexpensive and high-yield production method for an ?-L-aspartyl-L-phenylalanine-?-methyl ester is provided. ?-L-aspartyl-L-phenylalanine-?-methyl ester is produced from a L-aspartic acid-?,?-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to catalyze a reaction in which L-phenylalanine performs no nucleophilic attack on a ?-ester site of L-aspartic acid-?,?-diester but performs a nucleophilic attack on an ?-ester site thereof.Type: GrantFiled: January 7, 2008Date of Patent: June 29, 2010Assignee: Ajinomoto Co., Inc.Inventors: Kenzo Yokozeki, Ayako Ohno, Seiichi Hara, Isao Abe
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Patent number: 7736871Abstract: DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.Type: GrantFiled: August 20, 2007Date of Patent: June 15, 2010Assignee: Ajinomoto Co., Inc.Inventors: Seiichi Hara, Kenzo Yokozeki, Isao Abe, Naoto Tonouchi, Yasuko Jojima
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Patent number: 7736876Abstract: DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.Type: GrantFiled: June 18, 2008Date of Patent: June 15, 2010Assignee: Ajinomoto Co., Inc.Inventors: Seiichi Hara, Kenzo Yokozeki, Isao Abe, Naoto Tonouchi, Yasuko Jojima
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Publication number: 20090191600Abstract: A method for producing an ?-L-aspartyl-L-phenylalanine-?-ester (also named ?-L-(?-o-substituted aspartyl)-L-phenylalanine), which is an intermediate of an ?-L-aspartyl-L-phenylalanine-?-methyl ester (also named ?-L-aspartyl-L-phenylalanine methyl ester; product name: aspartame), easily, at high yield and inexpensively without going through a complex synthesis method is provided. Further, an easy, inexpensive and high-yield production method for an ?-L-aspartyl-L-phenylalanine-?-methyl ester is provided. ?-L-aspartyl-L-phenylalanine-?-methyl ester is produced from a L-aspartic acid-?,?-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to catalyze a reaction in which L-phenylalanine performs no nucleophilic attack on a ?-ester site of L-aspartic acid-?,?-diester but performs a nucleophilic attack on an ?-ester site thereof.Type: ApplicationFiled: January 7, 2008Publication date: July 30, 2009Applicant: Ajinomoto Co., Inc.Inventors: Kenzo YOKOZEKI, Ayako Ohno, Seiichi Hara, Isao Abe
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Patent number: 7531340Abstract: DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.Type: GrantFiled: August 20, 2007Date of Patent: May 12, 2009Assignee: AJINOMOTO Co., Inc.Inventors: Seiichi Hara, Kenzo Yokozeki, Isao Abe, Naoto Tonouchi, Yasuko Jojima
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Publication number: 20080274530Abstract: DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.Type: ApplicationFiled: June 18, 2008Publication date: November 6, 2008Applicant: Ajinomoto Co., Inc.Inventors: Seiichi HARA, Kenzo Yokozeki, Isao Abe, Naoto Tonouchi, Yasuko Jojima
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Publication number: 20080171357Abstract: A method is disclosed that allows the production of peptides having three or more amino acid residues easily, inexpensively and at high yield without going through a complex synthesis method. A novel enzyme that efficiently produces a peptide from bacteria belonging to the genus Empedobacter or the genus Sphingobacterium is provided. The enzyme acts on a carboxy component and an amine component to form peptides having three or more amino acid residues by acting on a carboxy component and an amine component.Type: ApplicationFiled: November 15, 2007Publication date: July 17, 2008Applicant: AJINOMOTO CO., INC.Inventors: Kenzo YOKOZEKI, Sonoko SUZUKI, Seiichi HARA, Isao ABE
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Publication number: 20080166761Abstract: DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.Type: ApplicationFiled: August 20, 2007Publication date: July 10, 2008Applicant: Ajinomoto Co., Inc.Inventors: Seiichi HARA, Kenzo Yokozeki, Isao Abe, Naoto Tonouchi, Yasuko Jojima
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Patent number: 7361458Abstract: A method for producing an ?-L-aspartyl-L-phenylalanine-?-ester (also named ?-L-(?-o-substituted aspartyl)-L-phenylalanine), which is an intermediate of an ?-L-aspartyl-L-phenylalanine-?-methyl ester (also named ?-L-aspartyl-L-phenylalanine methyl ester; product name: aspartame), easily, at high yield and inexpensively without going through a complex synthesis method is provided. Further, an easy, inexpensive and high-yield production method for an ?-L-aspartyl-L-phenylalanine-?-methyl ester is provided. ?-L-aspartyl-L-phenylalanine-?-methyl ester is produced from a L-aspartic acid-?,?-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to catalyze a reaction in which L-phenylalanine performs no nucleophilic attack on a ?-ester site of L-aspartic acid-?,?-diester but performs a nucleophilic attack on an ?-ester site thereof.Type: GrantFiled: June 28, 2004Date of Patent: April 22, 2008Assignee: Ajinomoto Co., Inc.Inventors: Kenzo Yokozeki, Ayako Ohno, Seiichi Hara, Isao Abe
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Patent number: 7354746Abstract: The present invention relates to a method for producing optically active IHOG, which can in turn be used for the production of monatin. The present invention further relates to a method for producing optically active monatin, and an aldolase which can be used in these methods. As such, the present invention enables the synthesis of 4-(Indole-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid with high optical purity, which is useful as an intermediate in the synthesis of optically active monatin, from indole pyruvic acid and pyruvic acid (or oxaloacetic acid).Type: GrantFiled: December 11, 2006Date of Patent: April 8, 2008Assignee: Ajinomoto Co., Inc.Inventors: Shunichi Suzuki, Seiichi Hara, Toshiki Taba, Yasuaki Takakura
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Patent number: 7338780Abstract: A method is disclosed that allows the production of peptides having three or more amino acid residues easily, inexpensively and at high yield without going through a complex synthesis method. A novel enzyme that efficiently produces a peptide from bacteria belonging to the genus Empedobacter or the genus Sphingobacterium is provided. The enzyme acts on a carboxy component and an amine component to form peptides having three or more amino acid residues by acting on a carboxy component and an amine component.Type: GrantFiled: May 21, 2004Date of Patent: March 4, 2008Assignee: Ajinomoto Co., Inc.Inventors: Kenzo Yokozeki, Sonoko Suzuki, Seiichi Hara, Isao Abe
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Publication number: 20080050773Abstract: DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.Type: ApplicationFiled: August 20, 2007Publication date: February 28, 2008Applicant: Ajinomoto Co., Inc.Inventors: Seiichi HARA, Kenzo Yokozeki, Isao Abe, Naoto Tonouchi, Yasuko Jojima
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Publication number: 20070292916Abstract: The present invention aims at providing an excellent peptide-synthesizing protein and a method for efficiently producing a peptide. The peptide is synthesized by reacting an amine component and a carboxy component in the presence of at least one of proteins shown in the following (I) and (II). (I) The mutant protein having an amino acid sequence comprising one or more mutations from any of the mutations 1 to 68, and the mutations 239 to 290 and 324 to 377 in an amino acid sequence of SEQ ID NO:2.Type: ApplicationFiled: June 20, 2007Publication date: December 20, 2007Applicant: Ajinomoto Co., Inc.Inventors: Isao ABE, Rie Takeshita, Seiichi Hara, Sonoko Suzuki, Kenzo Yokozeki, Masakazu Sugiyama, Shunichi Suzuki, Kunihiko Watanabe, Nobuhisa Shimba, Takefumi Nakamura, Uno Tagami, Yuya Kodama, Hiromi Onoye, Reiko Yuuji, Eiichiro Suzuki, Tatsuki Kashiwagi, Ningchun Xu, Yuko Kai
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Patent number: 7288389Abstract: DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.Type: GrantFiled: May 28, 2004Date of Patent: October 30, 2007Assignee: Ajinomoto Co., Inc.Inventors: Seiichi Hara, Kenzo Yokozeki, Isao Abe, Naoto Tonouchi, Yasuko Jojima
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Patent number: 7278876Abstract: A connector includes two housings coupled with each other to hold a cable therebetween. One of the housings includes a cable receiving portion for receiving the cable, a restricting portion adjacent to the cable receiving portion, a first wall portion formed outside the restricting portion and defining a space between the first wall portion and the restricting portion, and a first engaging portion inwardly protruding from the first wall portion. Another of the housings includes a cable pressing portion for pressing the cable towards the cable receiving portion and the restricting portion, and a second wall portion formed adjacent to the cable pressing portion and inserted into the space. The second wall portion has a second engaging portion extending outward from the second wall portion. The first and the second engaging potions are engaged with each other to prevent the housings from separating from each other.Type: GrantFiled: December 26, 2006Date of Patent: October 9, 2007Assignees: Japan Aviation Electronics Industry, Limited, Honda Motor Co., Ltd.Inventors: Seiichi Hara, Kiminobu Tabata, Takeshi Kai, Tsutomu Fukui, Masaru Shinmura, Tadashi Inazu, Shigeki Takahashi