Patents by Inventor Sergei Svitashev

Sergei Svitashev has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20230323374
    Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed.
    Type: Application
    Filed: November 15, 2022
    Publication date: October 12, 2023
    Applicants: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC
    Inventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, ZHONGSEN LI, ZHAN-BIN LIU, L. ALEKSANDER LYZNIK, JINRUI SHI, SERGEI SVITASHEV, JOSHUA K. YOUNG
  • Patent number: 11773400
    Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell, without incorporating a selectable transgene marker. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed, without incorporating a selectable transgene marker. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a guide polynucleotide/Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell, without incorporating a selectable transgene marker.
    Type: Grant
    Filed: August 20, 2014
    Date of Patent: October 3, 2023
    Inventors: Andrew Mark Cigan, Saverio Carl Falco, Michael Lassner, Zhan-Bin Liu, Sergei Svitashev
  • Publication number: 20230279413
    Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed.
    Type: Application
    Filed: November 16, 2022
    Publication date: September 7, 2023
    Applicants: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC
    Inventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, ZHONGSEN LI, ZHAN-BIN LIU, L. ALEKSANDER LYZNIK, JINRUI SHI, SERGEI SVITASHEV, JOSHUA K. YOUNG
  • Publication number: 20230235342
    Abstract: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.
    Type: Application
    Filed: August 23, 2022
    Publication date: July 27, 2023
    Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E. I. DU PONT DE NEMOURS AND COMPANY
    Inventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, MICHAEL LASSNER, DEREK JANTZ, ZHONGSEN LI, ZHAN-BIN LIU, SERGEI SVITASHEV, JAMES JEFFERSON SMITH
  • Publication number: 20230235345
    Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed.
    Type: Application
    Filed: November 17, 2022
    Publication date: July 27, 2023
    Applicants: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC
    Inventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, ZHONGSEN LI, ZHAN-BIN LIU, L. ALEKSANDER LYZNIK, JINRUI SHI, SERGEI SVITASHEV, JOSHUA K. YOUNG
  • Publication number: 20230203517
    Abstract: Compositions and methods are provided for large scale manipulation of genomic regions and chromosomal engineering of plant genomes. Portions of chromosomes may be deleted, translocated, duplicated or inverted, which are useful for various applications in plant breeding programs that relate to recombination, crossovers, and genetic gain. Site-specific directed DNA breaks enhance targeted recombination frequencies, crossover efficiency and movement of large chromosomal segments in crop plant cells. CRISPR-Cas systems enable targeted chromosomal engineering of crop plants.
    Type: Application
    Filed: May 28, 2021
    Publication date: June 29, 2023
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: MARY J. FRANK, JUSTIN P. GERKE, JENELLE D. F. MEYER, SERGEI SVITASHEV
  • Publication number: 20230091338
    Abstract: Compositions and methods are provided for an inducible, high efficiency intra-genomic homologous recombination (IGHR) system in plants, that can be used for non-chimeric, heritable gene targeting. The advantage of IGHR approach to targeted integration is that every cell contains donor DNA and nuclease-encoding sequences, so that there are many potentially homology-directed targeting events that can occur during plant development.
    Type: Application
    Filed: February 22, 2021
    Publication date: March 23, 2023
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: AJITH ANAND, MAREN L ARLING, PIERLUIGI BARONE, HYEON-JE CHO, WILLIAM JAMES GORDON-KAMM, SANDEEP KUMAR, BRIAN LENDERTS, KEITH S LOWE, SERGEI SVITASHEV, XINLI EMILY WU
  • Publication number: 20230059309
    Abstract: Compositions and methods are provided for the excision and replacement of an endogenous polynucleotide, such as a gene, using CRISPR-Cas systems. In some aspects, the gene is flanked by specific nucleotides that are targets of homology-directed repair, for the insertion of a replacement polynucleotide.
    Type: Application
    Filed: January 5, 2021
    Publication date: February 23, 2023
    Applicant: Pioneer Hi-Bred International, Inc.
    Inventors: Huirong GAO, Sergei SVITASHEV
  • Patent number: 11459576
    Abstract: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.
    Type: Grant
    Filed: July 10, 2020
    Date of Patent: October 4, 2022
    Inventors: Andrew Mark Cigan, Saverio Carl Falco, Huirong Gao, Michael Lassner, Derek Jantz, Zhongsen Li, Zhan-Bin Liu, Sergei Svitashev, James Jefferson Smith
  • Publication number: 20220307006
    Abstract: Methods and compositions are provided for the improvement of homology-directed repair of a double strand break in a plant cell, using concatemers of heterologous polynucleotides that are flanked by sequences capable of sequence hybridization with a guide RNA. In some aspects, the double strand break is created by an RNA-guided Cas endonuclease. The homology-directed repair of the double-strand break may include incorporation of a heterologous polynucleotide, for example a gene encoding a trait of agronomic importance. The homology-directed repair of the double-strand break may occur as a result of template-directed repair using a heterologous polynucleotide as a repair template.
    Type: Application
    Filed: July 17, 2020
    Publication date: September 29, 2022
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: AMIT DAS, SATISH KANUGANTI, AMITABH MOHANTY, SERGEI SVITASHEV, VENKATA S. TAVVA
  • Publication number: 20220251587
    Abstract: Methods and compositions are provided for the improvement of double-strand-break-inducing agent activity in eukaryotic cells, through the usage of one or more morphogenic factors or developmental genes. The morphogenic factor may be provided to the same cell or to a different cell than that comprising or receiving the double-strand-break-inducing agent. The morphogenic factor may be provided to a cell as a polynucleotide composition on a recombinant vector, and may be placed on the vector outside of a T-DNA border. The morphogenic factor may be provided via an upregulation of an endogenous gene. The morphogenic factor, or the double-strand-break-inducing agent, may further comprise a cell penetrating peptide. The morphogenic factor may be co-introduced with a vector comprising RepA.
    Type: Application
    Filed: April 11, 2022
    Publication date: August 11, 2022
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: STEPHEN LAWRENCE GASIOR, WILLIAM JAMES GORDON-KAMM, KEITH S LOWE, DAVID J PETERSON, SERGEI SVITASHEV, JOSHUA K YOUNG
  • Patent number: 11332752
    Abstract: Methods and compositions are provided for the improvement of double-strand-break-inducing agent activity in eukaryotic cells, through the usage of one or more morphogenic factors or developmental genes. The morphogenic factor may be provided to the same cell or to a different cell than that comprising or receiving the double-strand-break-inducing agent. The morphogenic factor may be provided to a cell as a polynucleotide composition on a recombinant vector, and may be placed on the vector outside of a T-DNA border. The morphogenic factor may be provided via an upregulation of an endogenous gene. The morphogenic factor, or the double-strand-break-inducing agent, may further comprise a cell penetrating peptide. The morphogenic factor may be co-introduced with a vector comprising RepA.
    Type: Grant
    Filed: March 11, 2019
    Date of Patent: May 17, 2022
    Assignee: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: Stephen Lawrence Gasior, William James Gordon-Kamm, Keith S Lowe, David J Peterson, Sergei Svitashev, Joshua K Young
  • Publication number: 20210062201
    Abstract: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.
    Type: Application
    Filed: July 10, 2020
    Publication date: March 4, 2021
    Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E. I. DU PONT DE NEMOURS AND COMPANY
    Inventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, MICHAEL LASSNER, DEREK JANTZ, ZHONGSEN LI, ZHAN-BIN LIU, SERGEI SVITASHEV, JAMES JEFFERSON SMITH
  • Publication number: 20210010012
    Abstract: Methods and compositions are provided for the improvement of double-strand-break-inducing agent activity in eukaryotic cells, through the usage of one or more morphogenic factors or developmental genes. The morphogenic factor may be provided to the same cell or to a different cell than that comprising or receiving the double-strand-break-inducing agent. The morphogenic factor may be provided to a cell as a polynucleotide composition on a recombinant vector, and may be placed on the vector outside of a T-DNA border. The morphogenic factor may be provided via an upregulation of an endogenous gene. The morphogenic factor, or the double-strand-break-inducing agent, may further comprise a cell penetrating peptide. The morphogenic factor may be co-introduced with a vector comprising RepA.
    Type: Application
    Filed: March 11, 2019
    Publication date: January 14, 2021
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: STEPHEN LAWRENCE GASIOR, WILLIAM JAMES GORDON-KAMM, KEITH S LOWE, DAVID J PETERSON, SERGEI SVITASHEV, JOSHUA K YOUNG
  • Patent number: 10822610
    Abstract: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.
    Type: Grant
    Filed: June 13, 2018
    Date of Patent: November 3, 2020
    Inventors: Andrew Mark Cigan, Saverio Carl Falco, Huirong Gao, Michael Lassner, Derek Jantz, Zhongsen Li, Zhan-Bin Liu, Sergei Svitashev, James Jefferson Smith
  • Publication number: 20200157554
    Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed.
    Type: Application
    Filed: January 30, 2020
    Publication date: May 21, 2020
    Applicants: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, ZHONGSEN LI, ZHAN-BIN LIU, L. ALEKSANDER LYZNIK, JINRUI SHI, SERGEI SVITASHEV, JOSHUA K. YOUNG
  • Publication number: 20190040372
    Abstract: Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.
    Type: Application
    Filed: October 18, 2018
    Publication date: February 7, 2019
    Applicants: E. I. DUPONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: Ericka Bermudez, Andrew Mark Cigan, James J English, Saverio Carl Falco, Huirong Gao, Lu Liu, Zhan-Bin Liu, Azalea S Ong, Sergei Svitashev, Joshua K Young
  • Publication number: 20190040405
    Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed.
    Type: Application
    Filed: September 13, 2018
    Publication date: February 7, 2019
    Applicants: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, ZHONGSEN LI, ZHAN-BIN LIU, LESZEK ALEKSANDER LYZNIK, JINRUI SHI, SERGEI SVITASHEV, JOSHUA K. YOUNG
  • Publication number: 20180371479
    Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed.
    Type: Application
    Filed: August 3, 2018
    Publication date: December 27, 2018
    Applicants: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: Andrew Mark Cigan, Saverio Carl Falco, Huirong Gao, Zhongsen Li, Zhan-Bin Liu, L. Aleksander Lyznik, Jinrui Shi, Sergei Svitashev, Joshua K. Young
  • Patent number: 10150956
    Abstract: Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.
    Type: Grant
    Filed: January 10, 2018
    Date of Patent: December 11, 2018
    Assignees: E I DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED IINTERNATIONAL, INC.
    Inventors: Ericka Bermudez, Andrew Mark Cigan, James J English, Saverio Carl Falco, Huirong Gao, Lu Liu, Zhan-Bin Liu, Azalea S Ong, Sergei Svitashev, Joshua K Young