Abstract: The present application relates to transcriptome sequencing and biomolecule space information detection. Specifically, the present application relates to a method for positioning and labeling a nucleic acid molecule, a method for constructing a nucleic acid molecule library for transcriptome sequencing, and a kit for implementing the method.

Abstract: The present disclosure describes various improvements for programmable nuclease-based detection assays. Also provided are compositions, methods, kits, systems, and devices for practicing the same. Such improvements include improved reporter designs to facilitate the cleavage of a reporter immobilized on a substrate by an activated programmable nuclease. Improved reporter designs may comprise various lengths and structures of the reporter. The substrate can also comprise immobilized guide nucleic acids and/or programmable nucleases.

Abstract: One or more embodiments of the disclosure are directed to methods of forming structures that are useful for FEOL and BEOL processes. Embodiments of the present disclosure advantageously provide methods of depositing a gapfill material, such as titanium nitride (TiN), in high aspect ratio (AR) structures with small dimensions. Some embodiments advantageously provide seam-free high-quality TiN films to fill high AR trenches with small dimensions. Embodiments of the present disclosure advantageously provide methods of filling 3D structures, such as FinFETs, GAAs, and the like, with a gapfill material without creating a seam. One or more embodiments include selective deposition processes using a carbon (C) layer in order to provide seam-free TiN gapfill in 3D structures, such as GAA devices.

Abstract: A method and apparatus for task information management, an electronic device and a computer readable storage medium are provided, and the present disclosure relates to the technical field of computer application, in particular to the field of information processing. A specific implementation solution is: generating task information according to a selected instant message, making a task creation interface pop up, and filling with recognized task information for editing by a user; and hooking a generated task in a dialog box in a form of a shortcut after creating the task. The task is created according to the selected instant message, so that manual operations of the user can be greatly reduced, it is quite convenient, a complicated step of looking for an entrance to a task information management function is omitted, and it is more convenient and faster to use.

Abstract: A flow electrode capacitive deionization system and a method for recovering phosphorus in phosphogypsum leachate and synchronous performing brine desalination belong to the technical field of wastewater treatment and recycling. The flow electrode capacitive deionization system includes a phosphorus recovery electrodeionization module and a desalination electrodeionization module. A first flow electrode solution reservoir, a phosphorus recovery electrodeionization module cathode flow electrode chamber, and a desalination electrodeionization module anode flow electrode chamber are interconnected in a circulation. A second flow electrode solution reservoir, a phosphorus recovery electrodeionization module anode flow electrode chamber, and a desalination electrodeionization module cathode flow electrode chamber are interconnected in a circulation. Two independent flow electrode solution circulation loops are formed.

Abstract: Provided herein are compositions, systems, and methods comprising effector proteins and uses thereof. These effector proteins may be characterized as CRISPR-associated (Cas) proteins. Various compositions, systems, and methods of the present disclosure may leverage the activities of these effector proteins for the modification, detection, and engineering of nucleic acids.

Abstract: Methods of manufacturing electronic devices, such as transistors (negative metal-oxide-semiconductor (NMOS) transistors (e.g., an N-metal stack) and positive metal-oxide-semiconductor (PMOS) transistors (e.g., a P-metal stack)) are described. Embodiments of the disclosure are directed to methods of improving PMOS transistor performance by inhibiting N-metal layer growth. The present disclosure provides two types of processes to reduce or inhibit N-metal layer growth. The disclosure provides methods which include forming a self-assembled monolayer (SAM) on the metal surface (e.g., titanium nitride (TiN)) of the PMOS, and methods which include forming a silicon-containing layer such as silicon oxide (SiOx) on the TiN surface. These two types of processes significantly reduce or inhibit the subsequent growth of an N-metal layer, such as titanium aluminum carbide (TiAlC), on the TiN surface of the PMOS.

Abstract: Provided are a method for positioning and labeling a nucleic acid molecule, and a method for constructing a nucleic acid molecule library for single-cell transcriptome sequencing, which relate to the technical fields of single-cell transcriptome sequencing and biomolecular space information detection. Further provided are a nucleic acid molecule library constructed by using the method, and a kit for implementing the method.

Abstract: Provided is a method for adjusting a target object, an electronic device, and a storage medium, relating to a field of computer technology, and in particular, to fields of intelligent transportation, automatic driving, computer vision, and the like. The method includes: acquiring a first bounding box corresponding to the target object and a second bounding box corresponding to a support of the target object; obtaining an association relationship for adjustment of the target object, according to a spatial position where the first and second bounding boxes are located; and moving, according to the association relationship, the target object towards the support, to obtain a third bounding box corresponding to the adjusted target object, where the third bounding box and the second bounding box appear to fit each other.

Abstract: A DNA library sequence for increasing the number of chip probes and an application thereof. The DNA library sequence comprises two or more position information labeling sequences and three or more fixed sequences, wherein the position information labeling sequences and the fixed sequences are arranged at intervals. The DNA library sequence increases the number of probes of a chip used for spatiotemporal omics, and then a capture number of transcript genes is increased. The present invention has a very high application prospect.

Abstract: Disclosed are an electrode plate and a battery. An electrode plate provided in the present disclosure includes a first current collector and a first functional layer disposed on at least one side of the first current collector, where a plurality of first regions and a plurality of second regions are alternately distributed on a surface of the first functional layer. A content ratio of an element oxygen to an element silicon in the first functional layer within the first region is greater than 4:1. The present disclosure provides the electrode plate and the battery, to at least resolve a problem of low initial efficiency of a lithium-ion battery, and increase battery capacity, thereby improving battery energy density.

Abstract: Disclosed are an electrode plate, a battery cell, and a battery. The electrode plate provided in the present disclosure includes a current collector and an active material layer disposed on at least one surface of the current collector. The active material layer includes active material, the active material includes a silicon-based material, a skeleton layer is provided outside the silicon-based material, and a proportion of a fluorine element gradually increases in a direction from the silicon-based material to the skeleton layer. The electrode plate, the battery cell, and the battery provided in the present disclosure are used to at least solve a technical problem that the battery is prone to volume expansion.

Abstract: Compositions and methods for improved SNP discrimination are provided. An exemplary composition comprises a programmable nuclease and a non-naturally occurring guide nucleic acid that hybridizes to a target nucleic acid, or segment thereof, comprising at least one single-nucleotide polymorphism (SNP). Methods may comprise a) contacting the sample to: i) a detector nucleic acid and ii) the composition; and b) assaying for a signal indicative of cleavage of the detector nucleic acid by the first programmable nuclease. In an exemplary embodiment, the target nucleic acid is a coronavirus target nucleic acid.

Abstract: The present application provides an electrode piece and a battery, wherein the electrode piece provided by the present application includes a first current collector and a first functional layer arranged on at least one surface of the first current collector, the first functional layer includes active material particles, and the surface of the first functional layer is provided with a plurality of first regions and a plurality of second regions distributed alternately with the first regions; an inorganic salt layer is arranged outside part of the active material particles in the first regions. The electrode piece and battery provided by the present application are used for improving initial efficiency and solving the problem of serious expansion of the battery.

Abstract: Various compositions and diagnostic devices for the detection of nucleic acids are provided. Also provided are systems comprising the same, and methods of using the same. In some embodiments, compositions and devices comprise or are configured to be used in combination with one or more of a guide nucleic acid, a programmable nuclease, and reporters. In some embodiments, presence of at least one sequence of interest is determined by detecting a signal produced upon cleavage of the reporters.

Abstract: The present disclosure provides various systems, diagnostic devices, and methods for nucleic acid analysis. The systems, devices, and methods allow for the analysis of nucleic acids, in a sample, via programmable nuclease-based assays. Provided are systems comprising an instrument and a cartridge allowing for point of care use. The systems, devices, and methods described herein can be configured for multiplexed detection of nucleic acids in a single sample.

Abstract: The present disclosure provides various diagnostic devices. Devices can comprising a sample interface configured to receive a sample that may comprise at least one sequence of interest; a channel in fluid communication with the sample interface and a detection chamber, said channel comprising one or more movable mechanisms to separate the sample into a plurality of droplets, wherein said detection chamber is configured to receive and contact the plurality of droplets with at least one programmable nuclease probe disposed on a surface of said detection chamber, and wherein said at least one programmable nuclease probe may comprise a guide nucleic acid complexed with a programmable nuclease; and a plurality of sensors that determine a presence of said at least one sequence of interest by detecting a signal produced upon cleavage of a target nucleic acid region of said at least one sequence of interest by said at least one programmable nuclease probe.

Abstract: The present disclosure provides compositions of effector proteins. The compositions may comprise engineered guide nucleic acids. Also disclosed are the methods and systems for detecting and modifying target nucleic acids using the same. The compositions may provide nucleic acid modification and/or detection at relatively high temperatures.

Abstract: The present disclosure provides an O-methyltransferase protein with a highly specific catalytic function for multiple benzylisoquinoline alkaloids (BIAs) parent nuclei and an encoding gene and use thereof. Compared with wild-type O-methyltransferase, the O-methyltransferase protein (SEQ ID NO: 2) shows an enhanced enzymatic activity and a wider substrate scope. The O-methyltransferase protein can catalyze O-methylation of backbones from three BIAs, including monobenzylisoquinolines (norcoclaurine, coclaurine, and N-methylcoclaurine), aporphines (asimilobine and N-methylasimilobine), and protoberberines (scoulerine and tetrahydrocolumbamine). Meanwhile, this O-methyltransferase protein is highly regioselective for each backbone. The O-methyltransferase protein catalyzes methylation of the monobenzylisoquinolines at 6-OH and 7-OH, is only active on 6-OH of the backbone of the aporphines, and mainly catalyzes methylation of the protoberberines at 2-OH.