Abstract: The present invention relates to novel monooxygenases which are useful in the hydroxylation of aromatic hydrocarbons. They are particularly useful for the production of 1-naththol and 7-hydroxycoumarin from naphthol and 7-Ethoxycoumarin, respectively.

Abstract: Multiple listlets function as a single master linked list to manage data packets across one or more banks of memory in a first-in first-out (FIFO) order, while allowing multiple push and/or pop functions to be performed per cycle. Each listlet can be a linked list that tracks pointers and is stored in a different memory bank. The nodes can include a pointer to a data packet, a pointer to the next node in the listlet and a next listlet identifier that identifies the listlet that contains the next node in the master linked list. The head and tail of each listlet, as well as an identifier each to track the head and tail of the master linked list, can be maintained in cache. The individual listlets are updated accordingly to maintain order of the master linked list as pointers are pushed and popped from the master linked list.

Abstract: Compositions and methods for improved SNP discrimination are provided. An exemplary composition comprises a programmable nuclease and a non-naturally occurring guide nucleic acid that hybridizes to a target nucleic acid, or segment thereof, comprising at least one single-nucleotide polymorphism (SNP). Methods may comprise a) contacting the sample to: i) a detector nucleic acid and ii) the composition; and b) assaying for a signal indicative of cleavage of the detector nucleic acid by the first programmable nuclease. In an exemplary embodiment, the target nucleic acid is a coronavirus target nucleic acid.

Abstract: The present application provides an electrode piece and a battery, wherein the electrode piece provided by the present application includes a first current collector and a first functional layer arranged on at least one surface of the first current collector, the first functional layer includes active material particles, and the surface of the first functional layer is provided with a plurality of first regions and a plurality of second regions distributed alternately with the first regions; an inorganic salt layer is arranged outside part of the active material particles in the first regions. The electrode piece and battery provided by the present application are used for improving initial efficiency and solving the problem of serious expansion of the battery.

Abstract: Various compositions and diagnostic devices for the detection of nucleic acids are provided. Also provided are systems comprising the same, and methods of using the same. In some embodiments, compositions and devices comprise or are configured to be used in combination with one or more of a guide nucleic acid, a programmable nuclease, and reporters. In some embodiments, presence of at least one sequence of interest is determined by detecting a signal produced upon cleavage of the reporters.

Abstract: The present disclosure provides various systems, diagnostic devices, and methods for nucleic acid analysis. The systems, devices, and methods allow for the analysis of nucleic acids, in a sample, via programmable nuclease-based assays. Provided are systems comprising an instrument and a cartridge allowing for point of care use. The systems, devices, and methods described herein can be configured for multiplexed detection of nucleic acids in a single sample.

Abstract: Multiple listlets function as a single master linked list to manage data packets across one or more banks of memory in a first-in first-out (FIFO) order, while allowing multiple push and/or pop functions to be performed per cycle. Each listlet can be a linked list that tracks pointers and is stored in a different memory bank. The nodes can include a pointer to a data packet, a pointer to the next node in the listlet and a next listlet identifier that identifies the listlet that contains the next node in the master linked list. The head and tail of each listlet, as well as an identifier each to track the head and tail of the master linked list, can be maintained in cache. The individual listlets are updated accordingly to maintain order of the master linked list as pointers are pushed and popped from the master linked list.

Abstract: The present disclosure provides compositions of effector proteins. The compositions may comprise engineered guide nucleic acids. Also disclosed are the methods and systems for detecting and modifying target nucleic acids using the same. The compositions may provide nucleic acid modification and/or detection at relatively high temperatures.

Abstract: The present disclosure provides various diagnostic devices. Devices can comprising a sample interface configured to receive a sample that may comprise at least one sequence of interest; a channel in fluid communication with the sample interface and a detection chamber, said channel comprising one or more movable mechanisms to separate the sample into a plurality of droplets, wherein said detection chamber is configured to receive and contact the plurality of droplets with at least one programmable nuclease probe disposed on a surface of said detection chamber, and wherein said at least one programmable nuclease probe may comprise a guide nucleic acid complexed with a programmable nuclease; and a plurality of sensors that determine a presence of said at least one sequence of interest by detecting a signal produced upon cleavage of a target nucleic acid region of said at least one sequence of interest by said at least one programmable nuclease probe.

Abstract: The present disclosure provides an O-methyltransferase protein with a highly specific catalytic function for multiple benzylisoquinoline alkaloids (BIAs) parent nuclei and an encoding gene and use thereof. Compared with wild-type O-methyltransferase, the O-methyltransferase protein (SEQ ID NO: 2) shows an enhanced enzymatic activity and a wider substrate scope. The O-methyltransferase protein can catalyze O-methylation of backbones from three BIAs, including monobenzylisoquinolines (norcoclaurine, coclaurine, and N-methylcoclaurine), aporphines (asimilobine and N-methylasimilobine), and protoberberines (scoulerine and tetrahydrocolumbamine). Meanwhile, this O-methyltransferase protein is highly regioselective for each backbone. The O-methyltransferase protein catalyzes methylation of the monobenzylisoquinolines at 6-OH and 7-OH, is only active on 6-OH of the backbone of the aporphines, and mainly catalyzes methylation of the protoberberines at 2-OH.

Abstract: Disclosed is a method for producing phycocyanobilin by use of a recombinant Escherichia coli that express heterologous heme oxygenase ho1 and ferredoxin oxidoreductase pcyA derived from Synechocystis sp. PCC6803. According to the present disclosure, heterologous expression of ho1 and pcyA genes leads to conversion of heme to an intermediate biliverdin for phycocyanobilin synthesis, and reduces the accumulation of biliverdin in the process of the phycocyanobilin synthesis. The genome of E. coli is further engineered to overexpress related genes of a metabolic pathway of phycocyanobilin, and a strain of recombinant E. coli with high yield of phycocyanobilin is obtained. The recombinant E. coli strain is cultured for 36 hr in a system using glycerol as a substrate, and the phycocyanobilin yield can reach 147 mg/L.

Abstract: The present disclosure discloses recombinant Escherichia coli for expressing a synthesis pathway of asiaticoside and application thereof, and belongs to the field of bioengineering. Rhamnosyltransferase with an amino acid sequence shown in any one of SEQ ID NO: 25 to SEQ ID NO: 29, glucosyltransferase with an amino acid sequence shown in any one of SEQ ID NO: 10 to SEQ ID NO: 17 and UGT73AH1 reported in a document are transferred into E. coli BL21 (DE3)?pgi to realize co-expression. Fermentation results show that all 5 rhamnosyltransferases screened in the present disclosure can achieve effects, and a unique new peak appears at 0.596 min, which is consistent with a characteristic ion flow of an asiaticoside standard product. According to the present disclosure, barriers of the prior art are broken through, a new biosynthesis method for asiaticoside is provided, and industrialization of biosynthesis of asiaticoside becomes possible.

Abstract: Described herein are methods, devices, and compositions for immobilization of biomolecules in diagnostic assays onto a surface. The methods, devices, and compositions may utilize or comprise a plurality of different non-naturally occurring guide nucleic acids. Each of the different non-naturally occurring guide nucleic acids may be immobilized to a surface at a known location identified with the particular non-naturally occurring guide nucleic acid, Alternatively, or in combination, a plurality of programmable nucleases may be immobilized to the surface at each of the known locations. Optionally, the methods, devices, and compositions may utilize or comprise a plurality of reporters immobilized to the surface in proximity to each of the different non-naturally occurring guide nucleic acids and/or programmable nucleases at each of the known locations.

Abstract: The present disclosure relates to a spheroidizing-annealed steel for the ball screw having high strength and resistance to low temperatures, wherein the chemical composition of the steel in mass percentage is: C: 0.40-0.70%, Si: 1.20-1.80%, Mn: 1.00-1.60%, Cr: 0.80-1.20%, S: ?0.025%, P?0.025%, Ni: 0.10-0.60%, Cu: 0.30-0.80%, Mo: 0.10-0.40%, Al?0.05%, Ca?0.0010%, Ti?0.003%, O?0.0010%, As?0.04%, Sn?0.03%, Sb?0.005%, Pb?0.002%, the balance is Fe and unavoidable impurities. In microstructure of the steel, cementite exists in a spheroidized state with a diameter of 0.1-0.5 ?m, preferably 0.3-0.5 ?m, a spheroidizing rate is 95% or more, and the rest is ferrite.

Abstract: The invention relates to the technical field of agricultural planting, in particular to a special controlled-release microbial fertilizer for peanuts in a continuous cropping field and a preparation method thereof. The fabric comprises an outer layer, a middle layer and an inner layer. Through a three-layer structure, the controlled-release fertilizer prepared by the disclosure can realize the fertilizer efficiency period of 50-60 days, and achieves the purpose of twice releases.

Abstract: The present disclosure provides single-buffer systems that enable efficient and rapid amplification and programmable nuclease enzyme mediated reactions. The single-buffer systems and reagent compositions therein may be used for assaying for a nucleic acid sequence from a sample.

Abstract: The present disclosure provides compositions and methods of use for a Type VI CRISPR/Cas nuclease. Type VI CRISPR/Cas nucleases are able to bind to a target nucleic acid, thereby activating trans-collateral nuclease activity on nucleic acid reports. Furthermore, the present disclosure provides methods to modify ribonucleic acid sequences using the programmable nucleases disclosed herein.

Abstract: Provided herein are programmable nucleases and methods of genome editing and detection of nucleic acids with said programmable nuclease. In some embodiments, the programmable nuclease is a programmable nickase.

Abstract: The application provides devices, kits and methods for a streamlined lateral flow assay for sample preparation, optional amplification, and detection of a target nucleic acid using programmable nuclease reagents.