Abstract: Described herein are devices, systems, fluidic devices, kits, and methods for detection of target nucleic acids associated with diseases, cancers, genetic disorders, a genotype, a phenotype, or ancestral origin. The devices, systems, fluidic devices, kits, and methods may comprise reagents of a guide nucleic acid targeting a target nucleic acid, a programmable nuclease, and a single stranded detector nucleic acid with a detection moiety. The target nucleic acid of interest may be indicative of a disease, and the disease may be communicable diseases, or of a cancer or genetic disorder. The target nucleic acid of interest may be indicative of a genotype, a phenotype, or ancestral origin.

Abstract: Described herein are devices, systems, fluidic devices, kits, and methods for detection of target nucleic acids associated with diseases, cancers, genetic disorders, a genotype, a phenotype, or ancestral origin. The devices, systems, fluidic devices, kits, and methods may comprise reagents of a guide nucleic acid targeting a target nucleic acid, a programmable nuclease, and a single stranded detector nucleic acid with a detection moiety. The target nucleic acid of interest may be indicative of a disease, and the disease may be communicable diseases, or of a cancer or genetic disorder. The target nucleic acid of interest may be indicative of a genotype, a phenotype, or ancestral origin.

Abstract: Described herein are devices, systems, fluidic devices, kits, and methods for detection of target nucleic acids associated with diseases, cancers, genetic disorders, a genotype, a phenotype, or ancestral origin. The devices, systems, fluidic devices, kits, and methods may comprise reagents of a guide nucleic acid targeting a target nucleic acid, a programmable nuclease, and a single stranded detector nucleic acid with a detection moiety. The target nucleic acid of interest may be indicative of a disease, and the disease may be communicable diseases, or of a cancer or genetic disorder. The target nucleic acid of interest may be indicative of a genotype, a phenotype, or ancestral origin.

Abstract: Provided herein are programmable nucleases and methods of genome editing and detection of nucleic acids with said programmable nuclease. In some embodiments, the programmable nuclease is a programmable nickase.

Abstract: Disclosed herein are compositions, kits, and methods related to improved Cas activity. Through compositions and kits disclosed herein and practice of methods disclosed herein, one attains improved Cas activity such as Cas12 activity relative to Cas proteins in the art such as LbCas12. Further described herein are methods to detect target nucleic acid using a programmable nuclease system. Often, the target nucleic acids are present in at low frequency in the sample. Provided herein are methods for enriching these target nucleic acids for detection. Also described herein are methods to insert a PAM sequence into a target sequence of interest for use in a detection comprising a programmable nuclease.

Abstract: Described herein are devices, systems, fluidic devices, kits, and methods for detection of target nucleic acids associated with diseases, cancers, genetic disorders, a genotype, a phenotype, or ancestral origin. The devices, systems, fluidic devices, kits, and methods may comprise reagents of a guide nucleic acid targeting a target nucleic acid, a programmable nuclease, and a single stranded detector nucleic acid with a detection moiety. The target nucleic acid of interest may be indicative of a disease, and the disease may be communicable diseases, or of a cancer or genetic disorder. The target nucleic acid of interest may be indicative of a genotype, a phenotype, or ancestral origin.

Abstract: Described herein are devices, systems, fluidic devices, kits, and methods for detection of target nucleic acids associated with diseases, cancers, genetic disorders, a genotype, a phenotype, or ancestral origin. The devices, systems, fluidic devices, kits, and methods may comprise reagents of a guide nucleic acid targeting a target nucleic acid, a programmable nuclease, and a single stranded detector nucleic acid with a detection moiety. The target nucleic acid of interest may be indicative of a disease, and the disease may be communicable diseases, or of a cancer or genetic disorder. The target nucleic acid of interest may be indicative of a genotype, a phenotype, or ancestral origin.

Abstract: Disclosed herein are compositions, kits, and methods related to improved Cas activity. Through compositions and kits disclosed herein and practice of methods disclosed herein, one attains improved Cas activity such as Cas12 activity relative to Cas proteins in the art such as LbCas12. Further described herein are methods to detect target nucleic acid using a programmable nuclease system. Often, the target nucleic acids are present in at low frequency in the sample. Provided herein are methods for enriching these target nucleic acids for detection. Also described herein are methods to insert a PAM sequence into a target sequence of interest for use in a detection comprising a programmable nuclease.

Abstract: Engineered CRISPR-Cas9 nucleases with improved specificity and their use in genomic engineering, epigenomic engineering, genome targeting, and genome editing.

Abstract: A light-emitting diode includes a first-type nitride region, a light-emitting region and a second-type nitride region, wherein the first-type nitride region includes a plurality of alternating first nitride layers and second nitride layers. The second nitride layers have high-doped emitting points pointing to the corresponding first nitride layer. The second-type nitride region includes a plurality of alternating third nitride layers and fourth nitride layers, wherein doping concentration of the fourth nitride layer is higher than that of the third nitride layer, and the fourth nitride layer has high-doped emitting points pointing to the third nitride layer.

Abstract: Example methods and devices for processing a page are described. One or more pages of a designated website are acquired. The one or more pages are clustered to obtain one or more classes in accordance with page features of the pages. At least one class is selected as a list page set according to a page linking relationship between the one or more classes. It is not necessary to require an operator to manually involve in the process of establishing the list page set. The present techniques have simple operations and high accuracy rate, thereby improving an efficiency and reliability of establishing a list page library.

Abstract: A light-emitting diode includes a first-type nitride region, a light-emitting region and a second-type nitride region, wherein the first-type nitride region includes a plurality of alternating first nitride layers and second nitride layers. The second nitride layers have high-doped emitting points pointing to the corresponding first nitride layer. The second-type nitride region includes a plurality of alternating third nitride layers and fourth nitride layers, wherein doping concentration of the fourth nitride layer is higher than that of the third nitride layer, and the fourth nitride layer has high-doped emitting points pointing to the third nitride layer.

Abstract: A concealed slide module is formed by installing an inner plate, at least one first ball plate, a middle plate, at least one second ball plate and an outer plate sequentially and installed between a bottom side of a drawer and a cupboard. The middle plate covers the exterior of the first ball plate and has an inner sidewall facing the inner plate and having a roof portion protruded horizontally outward from the top side of the middle plate, and a sliding section bent inwardly from a lower edge of the sidewall, and the sliding section is parallel to the platform, and a guide wheel is protruded from an outer sidewall at an end of the middle plate, and the middle plate slides with respect to the inner plate through the first ball plate.

Abstract: A concealed slide module is formed by installing an inner plate, at least one first ball plate, a middle plate, at least one second ball plate and an outer plate sequentially and installed between a bottom side of a drawer and a cupboard. The middle plate covers the exterior of the first ball plate and has an inner sidewall facing the inner plate and having a roof portion protruded horizontally outward from the top side of the middle plate, and a sliding section bent inwardly from a lower edge of the sidewall, and the sliding section is parallel to the platform, and a guide wheel is protruded from an outer sidewall at an end of the middle plate, and the middle plate slides with respect to the inner plate through the first ball plate.

Abstract: Example methods and devices for processing a page are described. One or more pages of a designated website are acquired. The one or more pages are clustered to obtain one or more classes in accordance with page features of the pages. At least one class is selected as a list page set according to a page linking relationship between the one or more classes. It is not necessary to require an operator to manually involve in the process of establishing the list page set. The present techniques have simple operations and high accuracy rate, thereby improving an efficiency and reliability of establishing a list page library.

Abstract: A label creating method for creating labels to be attached to checked fixtures comprises these steps: providing a label creating system (100), which includes a database module (40) storing relational parameters and data of the checked fixtures, and a server module (30) connected to the database module; sending acquiring instruction to the server module; acquiring relational parameters and data of a checked fixture stored in the database module; creating printing instruction according to the relational parameters and data; and printing labels according to the printing instruction.

Abstract: A label creating method for creating labels to be attached to checked fixtures comprises these steps: providing a label creating system (100), which includes a database module (40) storing relational parameters and data of the checked fixtures, and a server module (30) connected to the database module; sending acquiring instruction to the server module; acquiring relational parameters and data of a checked fixture stored in the database module; creating printing instruction according to the relational parameters and data; and printing labels according to the printing instruction.

Abstract: A system (100) for managing fixture calibration report includes a database (40) and a server (30). The database stores basic information reports and measurement reports of fixtures. The server is connected to the database and includes a calibration report generating module (301) configured to acquire relational data from the measurement reports, calculate and estimate the relational data acquired from the measurement report in order to generate a calibration report for each fixture, and an information transfer module (307) configured to obtain an electronic information account from the basic information reports. The calibration report generating module (301) is further configured for sending the calibration reports to the obtained electronic information account. A method for managing fixture calibration reports is also provided.

Abstract: A system (100) for managing electronic documents associated with respective products (i.e., product documentation) includes a database (40) and a server (30). The database selectively stores the electronic documents. The server is electronically connected to the database and includes a loading module (301) and a processing module (302). The loading module transmits selected electronic documents to the database. The processing module includes an inquiry module (3021) configured for locating/determining related documents in the database according to a keyword; a path-generating module (3022) configured for generating path links indicating locations of the located related documents; and a maintenance module (3023) for automatically deleting selectably chosen electronic documents in the database.

Abstract: A data access control system and a method thereof is disclosed. First, the master key is divided into n subkeys to be kept by n holders, by way of secret sharing. The master key reconstruction procedure utilizes the shared secret with the subkey holders to obtain m subkeys (1<m<n) and then reconstructs the master key, using the (m,n)-threshold scheme. The system then uses the master key to access the data. Thus, m subkeys are required to reconstruct the master key in order to amend or read the data in the system.