Abstract: Provided herein are methods of tracking molecular dynamics in three dimensions. In some embodiments, the methods include introducing an incident light toward a second surface of a substrate to induce a plasmonic wave at least proximal to a first surface of the substrate. A population of particles is connected to the first surface of the substrate via one or more first biomolecules. In some embodiments, the methods also include detecting a change in position of the particles in the population along at least three dimensions over a duration from a change in intensity of the incident light reflected at an interface of the first surface of the substrate. Related systems and computer readable media are also provided.

Abstract: Detecting single molecules includes binding the single molecules to a surface of an optically transparent substrate, irradiating the surface of the substrate with light having an incident angle selected to achieve total reflection of the light, thereby scattering light from the surface and from the single molecules bound to the surface, and collecting light scatted by the surface and by the single molecules bound to the surface to form a series of images. Systems for detecting single molecules include an optically transparent substrate, a means for flowing a sample solution over a surface of the substrate, a light source configured to irradiate the surface, a camera, and a collection optical system configured to collect light scatted by the surface and by the target molecules on the surface to form a series of images on the camera.

Abstract: Provided herein are methods of assessing the presence of microbes in a liquid sample that include assessing an initial integrated scattering intensity of objects (IC0) in the sample and an integrated scattering intensity of the objects at a time t (ICt) from modified images of the liquid sample, and identifying the sample as comprising microbes for (ICt)/(IC0) above a predefined infection threshold TI. Related systems and other aspects are also provided.

Abstract: A system for detecting target molecules includes a sample well defining a sensing region and two electrode regions, a sensor positioned in the sensing region and sensitized to the target molecules, an electrode positioned in each electrode region and configured to expose the sensor to a frequency-modulated electric field, and a detector configured to detect both an amplitude of oscillation of the sensor at a frequency of the modulated electric field and a direction of a displacement of the sensor. The sensing region defines a channel between the electrodes, and a ratio of a current density at a center of the sensing region to a current density at one of the electrodes is at least 2. The system allows detection of target molecules in a normal ionic strength buffer (e.g., having an ionic strength in a range of about 10 mM to about 1 M).

Abstract: A method for deep learning video microscopy-based antimicrobial susceptibility testing of a bacterial strain in a sample by acquiring image sequences of individual bacterial cells of the bacterial strain in a subject sample before, during, and after exposure to each antibiotic at different concentrations. The image sequences are compressed into static images while preserving essential phenotypic features. Data representing the static images is input into a pre-trained deep learning (DL) model which generates output data; and antimicrobial susceptibility for the bacterial strain is determined from the output data.

Abstract: A method and system for analysis of protein interaction kinetics in microarray or whole-cell based formats includes positioning a sensor chip on a prism. The sensor chip is spotted with a plurality of target molecules. A movable printer head deposits a plurality of analyte droplets on predefined regions of the sensor chip surface. A light source transmits light through the prism to excite surface plasmon resonance on the sensor chip surface, whereby the plurality of target molecules bound to the upper surface are changing the SPR resonance angle and therefore the intensity of the reflected beam. A detector receives reflected light transmitted through the prism from the bottom surface. Signals from the detector are received and processed into kinetic data and microarray labeled data to determine molecular interactions and binding kinetic properties for the plurality of analyte droplets.

Abstract: Method and system to remove background noise with a differential approach in optical imaging is disclosed. The differential approach moves the sample position laterally over a small distance, and a differential image is generated from the images recorded before and after the lateral translation. This approach can significantly improve the image quality of objects, including single DNA molecules, for label-free optical imaging techniques, such as surface plasmon resonance imaging. Disclosed imaging technique provides high-resolution genome-wide restriction maps of single DNA molecules.

Abstract: The disclosure discloses a method and device for forming a multi-cell beam. The method includes: a beam forming vector bq of each coordination cell in a heterogeneous network is calculated according to a principle of maximizing a signal intensity of a coordination cell scheduling user and minimizing a weighted interference leakage of the coordination cell scheduling user to other coordination cell scheduling users; and the beam forming vector bq of corresponding coordination cell is updated according to a calculation result, to enable each coordination cell to transmit data to a corresponding scheduling user according to updated beam forming vector bq.

Abstract: The present disclosure provides a method for detecting a random access signal. The method includes: determining a temporary peak detection sequence according to a received time domain random access signal; determining an interference cancellation weight for a frequency domain cyclic shift sequence corresponding to a search window in the temporary peak detection sequence, and performing interference cancellation on the temporary peak detection sequence according to the interference cancellation weight, obtaining a final peak detection sequence; and performing peak detection on the final peak detection sequence. The present disclosure discloses an apparatus and system for detecting a random access signal at the same time. According to the method, the present disclosure can eliminate the deterioration of the leak detection performance and the false detection performance caused by the interference during the random access signal detection, improve the detection accuracy and save resources.

Abstract: A method and device for selecting a heterogeneous network serving cell based on inter-cell cooperation, which relate to the field of digital communications. The method disclosed in the embodiments of the present invention comprises: acquiring, by a central node base station in a heterogeneous network, alternative users in each cell in the heterogeneous network; and according to a pre-set alternative user selection criterion, selecting an alternative user in the entire network from the alternative users in all cells in the heterogeneous network; and if the alternative user in the entire network needs to conduct a reselection operation, initiating a cell reselection flow. Also disclosed is a device for selecting a heterogeneous network serving cell based on inter-cell cooperation corresponding to the method.

Abstract: A method and device for selecting a heterogeneous network serving cell based on inter-cell cooperation, which relate to the field of digital communications. The method disclosed in the embodiments of the present invention comprises: acquiring, by a central node base station in a heterogeneous network, alternative users in each cell in the heterogeneous network; and according to a pre-set alternative user selection criterion, selecting an alternative user in the entire network from the alternative users in all cells in the heterogeneous network; and if the alternative user in the entire network needs to conduct a reselection operation, initiating a cell reselection flow. Also disclosed is a device for selecting a heterogeneous network serving cell based on inter-cell cooperation corresponding to the method.

Abstract: The disclosure discloses a method and device for forming a multi-cell beam. The method includes: a beam forming vector bq of each coordination cell in a heterogeneous network is calculated according to a principle of maximizing a signal intensity of a coordination cell scheduling user and minimizing a weighted interference leakage of the coordination cell scheduling user to other coordination cell scheduling users; and the beam forming vector bq of corresponding coordination cell is updated according to a calculation result, to enable each coordination cell to transmit data to a corresponding scheduling user according to updated beam forming vector bq.

Abstract: A method for detecting a random access signal includes: determining a temporary peak detection sequence according to a received time domain random access signal; determining an interference cancellation weight for a frequency domain cyclic shift sequence corresponding to a search window in the temporary peak detection sequence, and performing interference cancellation on the temporary peak detection sequence according to the interference cancellation weight, obtaining a final peak detection sequence; and performing peak detection on the final peak detection sequence. An apparatus and system for detecting a random access signal are also described.

Abstract: A method and system for analysis of protein interaction kinetics in microarray or whole-cell based formats includes positioning a sensor chip on a prism. The sensor chip is spotted with a plurality of target molecules. A movable printer head deposits a plurality of analyte droplets on predefined regions of the sensor chip surface. A light source transmits light through the prism to excite surface plasmon resonance on the sensor chip surface, whereby the plurality of target molecules bound to the upper surface are changing the SPR resonance angle and therefore the intensity of the reflected beam. A detector receives reflected light transmitted through the prism from the bottom surface. Signals from the detector are received and processed into kinetic data and microarray labeled data to determine molecular interactions and binding kinetic properties for the plurality of analyte droplets.

Abstract: A method, device and system for detecting a random access signal in an interference environment. The method includes: receiving a time-domain random access signal to obtain a frequency-domain random access signal; obtaining interference cancelling weights according to the frequency-domain random access signal and a frequency-domain local cyclic shift sequence, and performing interference cancelling on the frequency-domain random access signal with obtained interference cancelling weight; and performing peak detection on the frequency-domain random access signal after interference cancelling.

Abstract: Method and system to remove background noise with a differential approach in optical imaging is disclosed. The differential approach moves the sample position laterally over a small distance, and a differential image is generated from the images recorded before and after the lateral translation. This approach can significantly improve the image quality of objects, including single DNA molecules, for label-free optical imaging techniques, such as surface plasmon resonance imaging. Disclosed imaging technique provides high-resolution genome-wide restriction maps of single DNA molecules.

Abstract: The current invention provides methods for detecting trace analytes in solution or suspension using coupled micro-ring resonators. The methods of the current invention determine the presence and concentration of the target analytes in real time by exposing the micro-ring resonator to the analyte and determining the resulting rate of change of resonance wavelength experienced by a sensing micro-ring resonator as compared to a reference micro-ring resonator.

Abstract: The presently claimed and disclosed inventions relate, in general, to methods of radiation dosimetry and imaging using scintillation luminescence. More particularly, materials having a scintillation luminescence response to radiation that varies with total radiation dose received can be used for dosimetry monitoring, including, but not limited to nanoparticles for in vivo, real-time dosimetry. Energy-transfer nanocomposite materials as well as methods of making and using such materials in various applications including, but not limited to, in vivo radiation dosimetry and imaging, are disclosed. More particularly, the presently claimed and disclosed inventions relate to nanoparticle scintillation luminescence particles encapsulated in hosts of the general formula BaFX and BaFX:Eu2+ where X?Cl, Br and I.

Abstract: The present invention relates to a reaction or separation device, wherein the materials circularly flow in a container and a pipe. Said device contains a container and an external circular pipe, wherein the container is a double-chamber structure. The inner chamber consists of an outer cylinder and an inner cylinder with the latter being jacketed by the former, and there is a space between the walls of the outer cylinder and the inner cylinder. The lower end of the outer cylinder is open and higher than the bottom of the container, and the outer cylinder is fixed to the wall or bottom of the container. The upper end of the inner cylinder is open and its lower end connects with the external circular pipe through the feed outlet.

Abstract: Intermediates useful in the preparation of the antiviral agent entecavir are disclosed. A resin adsorption process for the isolation and purification of entecavir is also disclosed. Various processes useful in the preparation of entecavir are also disclosed.