Patents by Inventor Shawn A. Defrees

Shawn A. Defrees has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Production of Oligosaccharides By Microorganisms
    Publication number: 20080145899
    Abstract: The present invention relates to the enzymatic synthesis of oligosaccharides, including sialylated product saccharides. In particular, it relates to the use of recombinant cells to take up low cost precursors such as glucose, pyruvate and N-actyl-glucosamine, and to synthesize activated sugar moieties that are used in oligosaccharide synthesis. The methods make possible the synthesis of many oligosaccharides using microorganisms and readily available, relatively inexpensive starting materials.
    Type: Application
    Filed: September 19, 2005
    Publication date: June 19, 2008
    Applicant: Neose Technologies Inc
    Inventors: Karl Johnson, Noel J. Byrne, Shawn DeFrees
  • Glycolipids
    Publication number: 20080125392
    Abstract: Novel synthetic gangliosides and pharmaceutical compositions containing such synthetic gangliosides are described. Methods of making the novel synthetic ganglioside compounds and compositions as well as their use in the field of neuroprotection is also described.
    Type: Application
    Filed: November 3, 2005
    Publication date: May 29, 2008
    Applicant: NEOSE TECHNOLOGIES, INC.
    Inventors: Shawn Defrees, Zhi-Guang Wang
  • Glycopeptide remodeling using amidases
    Patent number: 7368108
    Abstract: This invention provides methods for modifying glycosylation patterns of glycopeptides, including recombinantly produced glycopeptides. Also provided are glycopeptide compositions in which the glycopeptides have a homogeneous glycosylation pattern.
    Type: Grant
    Filed: November 27, 2002
    Date of Patent: May 6, 2008
    Assignee: Neose Technologies, Inc.
    Inventors: Shawn DeFrees, Karl F. Johnson
  • Compositions and Methods for the Preparation of Human Growth Hormone Glycosylation Mutants
    Publication number: 20080102083
    Abstract: The present invention relates to mutants of human growth hormone, which contain newly introduced N-linked or O-linked glycosylation site(s), such that these recombinantly produced polypeptides have glycosylation patterns distinctly different from that of the naturally occurring human growth hormone. The polynucleotide coding sequences for the mutants, expression cassettes comprising the coding sequences, cells expressing the mutants, and methods for producing the mutants are also disclosed. Further disclosed are pharmaceutical compositions comprising the mutants and method for using the mutants.
    Type: Application
    Filed: May 7, 2004
    Publication date: May 1, 2008
    Applicant: NEOSE TECHNOLOGIES, INC.
    Inventors: Shawn DeFrees, Henrik Clausen
  • Factor VIII: Remodeling and glycoconjugation of factor VIII
    Publication number: 20080070275
    Abstract: The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide.
    Type: Application
    Filed: December 26, 2006
    Publication date: March 20, 2008
    Applicant: Neose Technologies, Inc.
    Inventors: Shawn DeFrees, David Zopf, Robert Bayer, Caryn Bowe, David Hakes, Xi Chen
  • O-linked glycosylation of peptides
    Patent number: 7338933
    Abstract: The present invention provides polypeptides that include an O-linked glycosylation site that is not present in the wild-type peptide. The polypeptides of the invention include glycoconjugates in which a species such as a water-soluble polymer, a therapeutic agent of a biomolecule is covalently linked through an intact O-linked glycosyl residue to the polypeptide. Also provided are methods of making the peptides of the invention and methods, pharmaceutical compositions containing the peptides and methods of treating, ameliorating or preventing diseased in mammals by administering an amount of a peptide of the invention sufficient to achieve the desired response.
    Type: Grant
    Filed: January 10, 2005
    Date of Patent: March 4, 2008
    Assignee: Neose Technologies, Inc.
    Inventors: Shawn DeFrees, David A. Zopf, Zhi-Guang Wang, Henrik Clausen
  • Granulocyte colony stimulating factor: remodeling and glycoconjugation of G-CSF
    Publication number: 20080050772
    Abstract: The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide.
    Type: Application
    Filed: January 11, 2007
    Publication date: February 28, 2008
    Applicant: Neose Technologies, Inc.
    Inventors: Shawn DeFrees, David Zopf, Robert Bayer, Caryn Bowe, David Hakes, Xi Chen
  • Glycosyltransferase inhibitors
    Patent number: 7323316
    Abstract: This invention provides potent inhibitors of glycosyltransferases. The glyscosyltransferase inhibitors are useful for inhibiting the synthesis of glycosides. Accordingly, the glycosyltransferase inhibitors find use, for example, in the modulation of biological processes that involve glycoside-mediated cell adhesion.
    Type: Grant
    Filed: September 8, 2003
    Date of Patent: January 29, 2008
    Assignee: Abaron Biosciences, Inc.
    Inventor: Shawn DeFrees
  • Glycopegylated Follicle Stimulating Hormone
    Publication number: 20080015142
    Abstract: The present invention provides conjugates between follicle stimulating hormone and PEG moieties. The conjugates are linked via an intact glycosyl linking group that is interposed between and covalently attached to the peptide and the modifying group. The conjugates are formed from both glycosylated and unglycosylated peptides by the action of a glycosyltransferase. The glycosyltransferase ligates a modified sugar moiety onto either an amino acid or glycosyl residue on the peptide. Also provided are pharmaceutical formulations including the conjugates. Methods for preparing the conjugates are also within the scope of the invention.
    Type: Application
    Filed: December 3, 2004
    Publication date: January 17, 2008
    Inventors: Shawn DeFrees, Robert J. Bayer, Caryn Bowe
  • Methods and Compositions for the Enzymatic Synthesis of Gangliosides
    Publication number: 20070275908
    Abstract: Novel synthetic glycosphingolipids and pharmaceutical compositions containing such synthetic glycosphingolipids are described. Methods of making the novel synthetic glycosphingolipid compounds and compositions as well as their use in the field of neuroprotection and cancer treatment is also described.
    Type: Application
    Filed: March 4, 2004
    Publication date: November 29, 2007
    Applicant: Neose Technologies, Inc.
    Inventors: Shawn Defrees, Karl Johnson, Zhi-Guang Wang
  • Methods of Refolding Mammalian Glycosyltranferases
    Publication number: 20070269879
    Abstract: The present invention provides methods of refolding mammalian glycosyltransferases that have been produced in bacterial cells, and methods to use such refolded glycosyltransferases, including glycosyltransferase mutants that have enhanced ability to be refolded. The invention also provides methods of refolding more than one glycosyltransferase in a single vessel, methods to use such refolded glycosyltransferases, and reaction mixtures comprising the refolded glycosyltransferases.
    Type: Application
    Filed: February 4, 2005
    Publication date: November 22, 2007
    Applicant: Neose Technologies, Inc.
    Inventors: Sami Saribas, David Hakes, Scott Willett, Karl Johnson, Daniel Bezila, Shawn Defrees
  • Interferon alpha: remodeling and glycoconjugation of interferon alpha
    Patent number: 7297511
    Abstract: The invention includes a multitude of methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide.
    Type: Grant
    Filed: April 9, 2003
    Date of Patent: November 20, 2007
    Assignee: Neose Technologies, Inc.
    Inventors: Shawn DeFrees, David Zopf, Robert Bayer, Caryn Bowe, David Hakes, Xi Chen
  • Glycopegylated Erythropoietin
    Publication number: 20070254834
    Abstract: The present invention provides conjugates between erythropoietin and PEG moieties. The conjugates are linked via an intact glycosyl linking group interposed between and covalently attached to the peptide and the modifying group. The conjugates are formed from glycosylated peptides by the action of a glycosyltransferase. The glycosyltransferase ligates a modified sugar moiety onto a glycosyl residue on the peptide. Also provided are methods for preparing the conjugates, methods for treating various disease conditions with the conjugates, and pharmaceutical formulations including the conjugates.
    Type: Application
    Filed: November 24, 2004
    Publication date: November 1, 2007
    Inventors: Shawn DeFrees, Robert Bayer, David Zopf
  • Glycopegylated Granulocyte Colony Stimulating Factor
    Publication number: 20070254836
    Abstract: The present invention provides conjugates between Granulocyte Colony Stimulating Factor and PEG moieties. The conjugates are linked via an intact glycosyl linking group that is interposed between and covalently attached to the peptide and the modifying group. The conjugates are formed from both glycosylated and unglycosylated peptides by the action of a glycosyltransferase. The glycosyltransferase ligates a modified sugar moiety onto either an amino acid or glycosyl residue on the peptide. Also provided are pharmaceutical formulations including the conjugates. Methods for preparing the conjugates are also within the scope of the invention.
    Type: Application
    Filed: December 3, 2004
    Publication date: November 1, 2007
    Inventors: Shawn Defrees, Henrik Clausen, David Zopf, Zhi-Guang Wang, Caryn Bowe, Marc Schwartz, Bingyuan Wu
  • Remodeling and glycoconjugation of peptides
    Patent number: 7276475
    Abstract: The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group a peptide.
    Type: Grant
    Filed: April 12, 2006
    Date of Patent: October 2, 2007
    Assignee: Neose Technologies, Inc.
    Inventors: Shawn DeFrees, David A. Zopf, Caryn Bowe
  • Glycopegylation methods and proteins/peptides produced by the methods
    Patent number: 7265084
    Abstract: The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide.
    Type: Grant
    Filed: April 9, 2003
    Date of Patent: September 4, 2007
    Assignee: Neose Technologies, Inc.
    Inventors: Shawn DeFrees, David A. Zopf, Robert J. Bayer, Caryn Bowe, David James Hakes, Xi Chen
  • Glycoconjugation methods and proteins/peptides produced by the methods
    Patent number: 7265085
    Abstract: The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide.
    Type: Grant
    Filed: April 9, 2003
    Date of Patent: September 4, 2007
    Assignee: Neose Technologies, Inc.
    Inventors: Shawn DeFrees, David Zopf, Robert Bayer, Caryn Bowe, David Hakes, Xi Chen
  • Compositions and methods for the preparation of protease resistant human growth hormone glycosylation mutants
    Publication number: 20070154992
    Abstract: The present invention relates to protease resistant mutants of human growth hormone, which contain newly introduced proteolysis resistant mutations and N-linked or O-linked glycosylation site(s), such that these recombinantly produced polypeptides have glycosylation patterns distinctly different from that of the naturally occurring human growth hormone. The polynucleotide coding sequences for the mutants, expression cassettes comprising the coding sequences, cells expressing the mutants, and methods for producing the mutants are also disclosed. Further disclosed are pharmaceutical compositions comprising the mutants and method for using the mutants.
    Type: Application
    Filed: April 10, 2006
    Publication date: July 5, 2007
    Applicant: NEOSE TECHNOLOGIES, INC.
    Inventor: Shawn DeFrees
  • Interferon beta: remodeling and glycoconjugation of interferon beta
    Patent number: 7226903
    Abstract: The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide.
    Type: Grant
    Filed: April 9, 2003
    Date of Patent: June 5, 2007
    Assignee: Neose Technologies, Inc.
    Inventors: Shawn DeFrees, David Zopf, Robert Bayer, Caryn Bowe, David Hakes, Xi Chen
  • One pot desialylation and glycopegylation of therapeutic peptides
    Publication number: 20070105755
    Abstract: The present invention provides conjugates between peptides and PEG moieties. The conjugates are linked via an intact glycosyl linking group that is interposed between and covalently attached to the peptide and the modifying group. The conjugates are formed from both glycosylated and unglycosylated peptides by the action of a glycosyltransferase. The glycosyltransferase ligates a modified sugar moiety onto either an amino acid or glycosyl residue on the peptide. Also provided are pharmaceutical formulations including the conjugates. Methods for preparing the conjugates are also within the scope of the invention.
    Type: Application
    Filed: October 13, 2006
    Publication date: May 10, 2007
    Applicant: Neose Technologies, Inc.
    Inventors: Shawn DeFrees, David Zopf, Susann Taudte, Walter Willett, Robert Bayer, Matthew Kalo