Patents by Inventor Shawn A. Defrees

Shawn A. Defrees has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • EXPRESSION OF SOLUBLE THERAPEUTIC PROTEINS
    Publication number: 20090298121
    Abstract: The present invention provides enhanced methods of producing soluble, active fibroblast growth factor-20 (FGF-20), FGF-21, neurotrophin-3 (NT-3), growth hormone (GH), granulocyte colony stimulating factor (G-CSF), or glucocerebrosidase proteins in microorganisms that have an oxidizing environment.
    Type: Application
    Filed: September 6, 2006
    Publication date: December 3, 2009
    Inventors: Marc F. Schwartz, Bingyuan Wu, Aliakbar Mobasseri, Shawn A. DeFrees, Tarik Soliman, Karl F. Johnson
  • Enzymatic modification of glycopeptides
    Publication number: 20090292110
    Abstract: The present invention provides glycoconjugates that are formed through the enzymatically-mediated coupling of a glycosyl moiety, e.g., on a peptide or lipid, and a modifying group that includes an acyl group. The conjugates include the modifying group tethered to the glycosyl moiety through a linking moiety that includes an acyl residue.
    Type: Application
    Filed: July 25, 2005
    Publication date: November 26, 2009
    Inventor: Shawn Defrees
  • Glycopegylated Granulocyte Colony Stimulating Factor
    Publication number: 20090203579
    Abstract: The present invention provides conjugates between Granulocyte Colony Stimulating Factor and PEG moieties. The conjugates are linked via an intact glycosyl linking group that is interposed between and covalently attached to the peptide and the modifying group. The conjugates are formed from both glycosylated and unglycosylated peptides by the action of a glycosyltransferase. The glycosyltransferase ligates a modified sugar moiety onto either an amino acid or glycosyl residue on the peptide. Also provided are pharmaceutical formulations including the conjugates. Methods for preparing the conjugates are also within the scope of the invention.
    Type: Application
    Filed: January 10, 2006
    Publication date: August 13, 2009
    Inventors: Shawn Defrees, Henrik Clausen, David A. Zopf, Caryn Bowe, Susann Taudte, Michael Felo, Walter S. Willett
  • SOLVENTS FOR MUTANT ENDOGLYCOCERAMIDASES WITH SYNTHETIC ACTIVITY
    Publication number: 20090176278
    Abstract: The present invention provides reaction mixtures comprising a solvent having at least one of an alkoxy ether and/or a polyhydric alcohol for use in reactions with a mutant endoglycoceramidase having enhanced synthetic activity.
    Type: Application
    Filed: December 1, 2006
    Publication date: July 9, 2009
    Applicant: NEOSE TECHNOLOGIES, INC.
    Inventors: Shawn Defrees, Xiaoping Tang
  • O-linked glycosylation of peptides
    Publication number: 20090169509
    Abstract: The present invention provides polypeptides that include an O-linked glycosylation site that is not present in the wild-type peptide. The polypeptides of the invention include glycoconjugates in which a species such as a water-soluble polymer, a therapeutic agent of a biomolecule is covalently linked through an intact O-linked glycosyl residue to the polypeptide. Also provided are methods of making the peptides of the invention and methods, pharmaceutical compositions containing the peptides and methods of treating, ameliorating or preventing diseased in mammals by administering an amount of a peptide of the invention sufficient to achieve the desired response.
    Type: Application
    Filed: January 10, 2005
    Publication date: July 2, 2009
    Inventors: Shawn DeFrees, David A. Zopf, Zhi-Guang Wang, Henrik Clausen
  • Mutant Endoglycoceramidases With Enhanced Synthetic Activity
    Publication number: 20090170155
    Abstract: The present invention relates to a novel endoglycoceramidase whose hydrolytic activity has been substantially reduced or eliminated, such that the enzyme is useful for synthesis of glycolipids from a monosaccharide or oligosaccharide and a ceramide. More specifically, the endoglycoceramidase is a mutant version of a naturally occurring endoglycoceramidase, preferably comprising a mutation within the active site or the nucleophilic site of the enzyme and more preferably comprising a substitution mutation of the Glu residue within the active site or the nucleophilic site. Also disclosed are a method for generating the mutant endoglycoceramidase and a method for enzymatically synthesizing glycolipids using this mutant enzyme.
    Type: Application
    Filed: June 1, 2005
    Publication date: July 2, 2009
    Applicant: THE UNIVERSITY OF BRITISH COLUMBIA UNIVERSITY-INDU
    Inventors: Karl F. Johnson, Shawn Defrees, Stephen Withers, Mark Vaughan
  • GLUCOSAMINE NUCLEOTIDE SUGARS
    Publication number: 20090137763
    Abstract: The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide.
    Type: Application
    Filed: April 11, 2008
    Publication date: May 28, 2009
    Applicant: Neose Technologies, Inc.
    Inventors: Shawn DeFrees, David Zopf, Robert Bayer, Caryn Bowe, David Hakes, Xi Chen
  • COMPOSITIONS AND METHODS FOR THE PREPARATION OF PROTEASE RESISTANT HUMAN GROWTH HORMONE GLYCOSYLATION MUTANTS
    Publication number: 20090124544
    Abstract: The present invention relates to protease resistant mutants of human growth hormone, which contain newly introduced proteolysis resistant mutations and N-linked or O-linked glycosylation site(s), such that these recombinantly produced polypeptides have glycosylation patterns distinctly different from that of the naturally occurring human growth hormone. The polynucleotide coding sequences for the mutants, expression cassettes comprising the coding sequences, cells expressing the mutants, and methods for producing the mutants are also disclosed. Further disclosed are pharmaceutical compositions comprising the mutants and method for using the mutants.
    Type: Application
    Filed: April 10, 2006
    Publication date: May 14, 2009
    Inventor: Shawn DeFrees
  • Glycopegylation methods and proteins/peptides produced by the methods
    Publication number: 20090093399
    Abstract: The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide.
    Type: Application
    Filed: January 24, 2007
    Publication date: April 9, 2009
    Applicant: Neose Technologies, Inc.
    Inventors: Shawn DeFrees, David A. Zopf, Robert J. Bayer, Caryn Bowe, David James Hakes, Xi Chen
  • GLYCOPEGYLATED FACTOR IX
    Publication number: 20090081188
    Abstract: The present invention provides conjugates between Factor IX and PEG moieties. The conjugates are linked via an intact glycosyl linking group interposed between and covalently attached to the peptide and the modifying group. The conjugates are formed from glycosylated peptides by the action of a glycosyltransferase. The glycosyltransferase ligates a modified sugar moiety onto a glycosyl residue on the peptide. Also provided are methods for preparing the conjugates, methods for treating various disease conditions with the conjugates, and pharmaceutical formulations including the conjugates.
    Type: Application
    Filed: August 1, 2008
    Publication date: March 26, 2009
    Applicant: Neose Technologies, Inc.
    Inventors: Shawn DeFrees, Robert J. Bayer, Caryn Bowe, Krishnasamy Panneerselvam
  • Lipo-Conjugation of Peptides
    Publication number: 20090054623
    Abstract: The present invention provides peptide conjugates that are formed between a modified lipid and a glycosyl residue and/or an amino acid residue on a peptide. The modified lipid includes a modifying group and a lipid linking group. Exemplary lipid linking groups include myristoyl, palmitoyl, and isoprenyl moieties.
    Type: Application
    Filed: December 19, 2005
    Publication date: February 26, 2009
    Inventor: Shawn DeFrees
  • C-, S- and N-glycosylation of peptides
    Publication number: 20090053167
    Abstract: The present invention provides polypeptide conjugates wherein a modifying group such as a water-soluble polymer, a therapeutic agent or a biomolecule is covalently linked to the polypeptide through a glycosyl linking group. In one embodiment, the polypeptide includes a glycosylation consensus sequence, wherein glycosylation occurs at an aromatic amino acid residue, such as the C-2 or the N-1 position of a tryptophan side chain. Exemplary polypeptides of the invention are those in which the glycosylation consensus sequence has been introduced into the amino acid sequence of the polypeptide by mutation. In another aspect the invention provides polypeptide conjugates wherein the modifying group is covalently linked to the polypeptide via a glycosyl mimetic linking group. Also provided are methods of making and using as well as pharmaceutical compositions containing the polypeptide conjugates of the invention.
    Type: Application
    Filed: May 14, 2008
    Publication date: February 26, 2009
    Applicant: Neose Technologies, Inc.
    Inventor: Shawn DeFrees
  • ST3Gal-1/ST6GalNAc-1 Chimeras
    Publication number: 20090047710
    Abstract: The present invention features compositions and methods related to increasing the solubility and enzymatic activity of GalNAc-?-2,6-sialyltransferase I (STÌGalNAcI) proteins expressed in prokaryotic host cells. Methods for increasing the solubility of STÌGalNAcI polypeptides include modifying cysteine residues, modifying N-linked glycosylation sites, deleting polypeptide regions, and constructing chimeric polypeptides comprising sequences from a STÌGalNAcI and another protein, for example, a Gal-?-1,3GalNAc-?-2,3-sialyltransferase (ST3GalI) and a STÌGalNAcI. The invention also features nucleic acids encoding such improved polypeptides, as well as vectors, host cells, expression systems, and methods of expressing and using such polypeptides.
    Type: Application
    Filed: November 8, 2006
    Publication date: February 19, 2009
    Applicant: Neose Technologies, Inc.
    Inventor: Shawn DeFrees
  • Nucleotide Sugar Purification Using Membranes
    Publication number: 20090048440
    Abstract: The invention provides methods of removing contaminants from a mixture of a desired product and contaminants by pH adjustments and molecular weight cut-offs. The contaminants include phosphate groups, magnesium sulfate, sodium pyruvate and tetrasodium pyrophosphate groups. The desired product includes nucleotide sugars, glycolipids, LnNT, sialyl lactose, and salts.
    Type: Application
    Filed: November 3, 2006
    Publication date: February 19, 2009
    Applicant: Neose Technologies, Inc.
    Inventors: Michael Felo, Shawn DeFrees
  • Glycopegylated Interferon Alpha
    Publication number: 20090028822
    Abstract: The present invention provides IFN-? conjugates including IFN-? peptides and modifying groups such as PEG moieties. The IFN-? peptide and modifying group are linked via an intact glycosyl linking group interposed between and covalently attached to the IFN-? peptide and the modifying group. The IFN-? conjugates are formed from glycosylated peptides by the action of a glycosyltransferase. The glycosyltransferase ligates a modified sugar onto an amino acid or a glycosyl residue on the IFN-? peptide. Also provided are methods for preparing the IFN-? conjugates, methods for treating various disease conditions with the IFN-? conjugates, and pharmaceutical formulations including the IFN-? conjugates.
    Type: Application
    Filed: September 12, 2005
    Publication date: January 29, 2009
    Applicant: NEOSE TECHNOLOGIES, INC.
    Inventors: Shawn DeFrees, David A. Zopf, Henrik Clausen, Ruye Xing
  • Remodeling and glycoconjugation of peptides
    Patent number: 7473680
    Abstract: The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group a peptide.
    Type: Grant
    Filed: July 15, 2005
    Date of Patent: January 6, 2009
    Assignee: Neose Technologies, Inc.
    Inventors: Shawn DeFrees, David A. Zopf, Robert J. Bayer, David James Hakes, Caryn Bowe, Xi Chen
  • Branched Polymeric Sugars and Nucleotides Thereof
    Publication number: 20080319183
    Abstract: The present invention provides sugars, nucleotide sugars, activated sugars that include one or more polymeric modifying moiety within their structure. The invention is exemplified by reference to linear and branched polymers, such as the water-soluble polymer poly(ethylene glycol).
    Type: Application
    Filed: January 26, 2005
    Publication date: December 25, 2008
    Applicant: NEOSE TECHNOLOGIES, INC.
    Inventors: Shawn DeFrees, Caryn Bowe
  • Glycopegylated Factor Ix
    Publication number: 20080318850
    Abstract: The present invention provides conjugates between Factor IX and PEG moieties. The conjugates are linked via an intact glycosyl linking group interposed between and covalently attached to the peptide and the modifying group. The conjugates are formed from glycosylated peptides by the action of a glycosyltransferase. The glycosyltransferase ligates a modified sugar moiety onto a glycosyl residue on the peptide. Also provided are methods for preparing the conjugates, methods for treating various disease conditions with the conjugates, and pharmaceutical formulations including the conjugates.
    Type: Application
    Filed: May 6, 2004
    Publication date: December 25, 2008
    Applicant: Neose Technologies, Inc.
    Inventors: Shawn DeFrees, Robert J. Bayer, Caryn Bowe, Krishnasamy Panneerselvam
  • Factor IX: remodeling and glycoconjugation of factor IX
    Publication number: 20080305991
    Abstract: The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide.
    Type: Application
    Filed: January 23, 2007
    Publication date: December 11, 2008
    Applicant: Neose Technologies, Inc.
    Inventors: Shawn DeFrees, David Zopf, Robert J. Bayer, Caryn Bowe, David Hakes, Xi Chen
  • Glycopegylated erythropoietin
    Publication number: 20080305992
    Abstract: The present invention provides conjugates between erythropoietin and PEG moieties. The conjugates are linked via an intact glycosyl linking group interposed between and covalently attached to the peptide and the modifying group. The conjugates are formed from glycosylated peptides by the action of a glycosyltransferase. The glycosyltransferase ligates a modified sugar moiety onto a glycosyl residue on the peptide. Also provided are methods for preparing the conjugates, methods for treating various disease conditions with the conjugates, and pharmaceutical formulations including the conjugates.
    Type: Application
    Filed: October 31, 2007
    Publication date: December 11, 2008
    Applicant: Neose Technologies, Inc.
    Inventors: Shawn DeFrees, Robert J. Bayer, David A. Zopf