Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.

Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.

Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.

Abstract: The specification describes a composition comprising an improved eukaryotic cell culture medium, which can be used for the production of a protein of interest. Taurine can be added to the serum-free media or chemically-defined media to increase the production of a protein of interest. Methods for recombinantly expressing high levels of protein using the media compositions are included.

Abstract: The instant application provides a method for screening batches of soy hydrolysate for a desired amount of a component thereof, such as ornithine or putrescine, and selecting only those batches of soy hydrolysate that have a desired amount of such component. The present disclosure also sets forth methods for culturing cells in media supplemented with selected batches of soy to produce more consistent, high quality lots of a protein of interest. Further, the present disclosure provides a plurality of protein preparations that have each been produced by culturing cells in media supplemented with separate batches of soy hydrolysate containing a desired amount of ornithine or putrescine, whereby each batch of protein produced exhibits improved quality of the protein of interest or amount of quality protein produced.

Abstract: Apparatus and techniques relating to data interface power consumption control are disclosed. Components of a data transfer module may be selectively moved between their normal operating states and reduced power states at times when the data transfer module is not to be used for transferring data. Decisions as to particular components that are to be moved to their reduced power states may be based on respective timing characteristics of the components and/or respective power consumption characteristics of the components, for example. In some embodiments, an action may be performed to reduce a powering up time of the data transfer module when normal operation of the data transfer module is to resume. In the case of a multiple-connection interface having respective data transfer modules for each connection, the interface may be partially shut down by moving a subset of the data transfer modules into reduced power states.

Abstract: An AC coupling circuit is provided that has a level shifter circuit having a p input voltage and an n input voltage and producing a p output voltage and a p output voltage. There is a common mode voltage adjustment feedback circuit configured to cause a common mode voltage output to tend towards a specified reference voltage, the common mode voltage output being an average of the p output and n output voltages of the level shifter circuit. In combination, the level shifter circuit and the feedback circuit allow the interconnection of a first circuit that operates at a first, unspecified, common mode voltage to be connected to a second circuit having a required common mode voltage. The level shifter may be formed of adjustable components such that the frequency response of the level shifter circuit can be adjusted to compensate for a frequency response of an interconnect between the first circuit and the second circuit.

Abstract: Improved methods for large scale production of proteins and/or polypeptides in cell culture is provided. In accordance with the present invention, the method provides for culturing cells that have metabolically shifted. The use of such a method or system allows high levels of protein or polypeptide production and reduces accumulation of unwanted metabolic waste such as lactate. Proteins and polypeptides expressed in accordance with the present invention may be advantageously used in the preparation of pharmaceutical, immunogenic, or other commercial biologic compositions, such as antibodies.

Abstract: Improved methods for large scale production of proteins and/or polypeptides in cell culture is provided. In accordance with the present invention, the method provides for culturing cells that have metabolically shifted. The use of such a method or system allows high levels of protein or polypeptide production and reduces accumulation of unwanted metabolic waste such as lactate. Proteins and polypeptides expressed in accordance with the present invention may be advantageously used in the preparation of pharmaceutical, immunogenic, or other commercial biologic compositions, such as antibodies.

Abstract: Apparatus and techniques relating to data interface power consumption control are disclosed. Components of a data transfer module may be selectively moved between their normal operating states and reduced power states at times when the data transfer module is not to be used for transferring data. Decisions as to particular components that are to be moved to their reduced power states may be based on respective timing characteristics of the components and/or respective power consumption characteristics of the components, for example. In some embodiments, an action may be performed to reduce a powering up time of the data transfer module when normal operation of the data transfer module is to resume. In the case of a multiple-connection interface having respective data transfer modules for each connection, the interface may be partially shut down by moving a subset of the data transfer modules into reduced power states.

Abstract: An AC coupling circuit is provided that has a level shifter circuit having a p input voltage and an n input voltage and producing a p output voltage and a p output voltage. There is a common mode voltage adjustment feedback circuit configured to cause a common mode voltage output to tend towards a specified reference voltage, the common mode voltage output being an average of the p output and n output voltages of the level shifter circuit. In combination, the level shifter circuit and the feedback circuit allow the interconnection of a first circuit that operates at a first, unspecified, common mode voltage to be connected to a second circuit having a required common mode voltage. The level shifter may be formed of adjustable components such that the frequency response of the level shifter circuit can be adjusted to compensate for a frequency response of an interconnect between the first circuit and the second circuit.

Abstract: An AC coupling circuit is provided that has a level shifter circuit having a p input voltage and an n input voltage and producing a p output voltage and a p output voltage. There is a common mode voltage adjustment feedback circuit configured to cause a common mode voltage output to tend towards a specified reference voltage, the common mode voltage output being an average of the p output and n output voltages of the level shifter circuit. In combination, the level shifter circuit and the feedback circuit allow the interconnection of a first circuit that operates at a first, unspecified, common mode voltage to be connected to a second circuit having a required common mode voltage. The level shifter may be formed of adjustable components such that the frequency response of the level shifter circuit can be adjusted to compensate for a frequency response of an interconnect between the first circuit and the second circuit.

Abstract: Apparatus and techniques relating to data interface power consumption control are disclosed. Components of a data transfer module may be selectively moved between their normal operating states and reduced power states at times when the data transfer module is not to be used for transferring data. Decisions as to particular components that are to be moved to their reduced power states may be based on respective timing characteristics of the components and/or respective power consumption characteristics of the components, for example. In some embodiments, an action may be performed to reduce a powering up time of the data transfer module when normal operation of the data transfer module is to resume. In the case of a multiple-connection interface having respective data transfer modules for each connection, the interface may be partially shut down by moving a subset of the data transfer modules into reduced power states.

Abstract: An AC coupling circuit is provided that has a level shifter circuit having a p input voltage and an n input voltage and producing a p output voltage and a p output voltage. There is a common mode voltage adjustment feedback circuit configured to cause a common mode voltage output to tend towards a specified reference voltage, the common mode voltage output being an average of the p output and n output voltages of the level shifter circuit. In combination, the level shifter circuit and the feedback circuit allow the interconnection of a first circuit that operates at a first, unspecified, common mode voltage to be connected to a second circuit having a required common mode voltage. The level shifter may be formed of adjustable components such that the frequency response of the level shifter circuit can be adjusted to compensate for a frequency response of an interconnect between the first circuit and the second circuit.

Abstract: Apparatus and techniques relating to data interface power consumption control are disclosed. Components of a data transfer module may be selectively moved between their normal operating states and reduced power states at times when the data transfer module is not to be used for transferring data. Decisions as to particular components that are to be moved to their reduced power states may be based on respective timing characteristics of the components and/or respective power consumption characteristics of the components, for example. In some embodiments, an action may be performed to reduce a powering up time of the data transfer module when normal operation of the data transfer module is to resume. In the case of a multiple-connection interface having respective data transfer modules for each connection, the interface may be partially shut down by moving a subset of the data transfer modules into reduced power states.

Abstract: Methods for manipulating carbohydrate processing pathways in cells of interest are provided. Methods are directed at manipulating multiple pathways involved with the sialylation reaction by using recombinant DNA technology and substrate feeding approaches to enable the production of sialylated glycoproteins in cells of interest. These carbohydrate engineering efforts encompass the implementation of new carbohydrate bioassays, the examination of a selection of insect cell lines and the use of bioinformatics to identify gene sequences for critical processing enzymes. The compositions comprise cells of interest producing sialylated glycoproteins. The methods and compositions are useful for heterologous expression of glycoproteins.

Abstract: Apparatus and techniques relating to data interface power consumption control are disclosed. Components of a data transfer module may be selectively moved between their normal operating states and reduced power states at times when the data transfer module is not to be used for transferring data. Decisions as to particular components that are to be moved to their reduced power states may be based on respective timing characteristics of the components and/or respective power consumption characteristics of the components, for example. In some embodiments, an action may be performed to reduce a powering up time of the data transfer module when normal operation of the data transfer module is to resume. In the case of a multiple-connection interface having respective data transfer modules for each connection, the interface may be partially shut down by moving a subset of the data transfer modules into reduced power states.

Abstract: Apparatus and techniques relating to data interface power consumption control are disclosed. Components of a data transfer module may be selectively moved between their normal operating states and reduced power states at times when the data transfer module is not to be used for transferring data. Decisions as to particular components that are to be moved to their reduced power states may be based on respective timing characteristics of the components and/or respective power consumption characteristics of the components, for example. In some embodiments, an action may be performed to reduce a powering up time of the data transfer module when normal operation of the data transfer module is to resume. In the case of a multiple-connection interface having respective data transfer modules for each connection, the interface may be partially shut down by moving a subset of the data transfer modules into reduced power states.

Abstract: Apparatus and techniques relating to data interface power consumption control are disclosed. Components of a data transfer module may be selectively moved between their normal operating states and reduced power states at times when the data transfer module is not to be used for transferring data. Decisions as to particular components that are to be moved to their reduced power states may be based on respective timing characteristics of the components and/or respective power consumption characteristics of the components, for example. In some embodiments, an action may be performed to reduce a powering up time of the data transfer module when normal operation of the data transfer module is to resume. In the case of a multiple-connection interface having respective data transfer modules for each connection, the interface may be partially shut down by moving a subset of the data transfer modules into reduced power states.

Abstract: Methods for manipulating carbohydrate processing pathways in cells of interest are provided. Methods are directed at manipulating multiple pathways involved with the sialylation reaction by using recombinant DNA technology and substrate feeding approaches to enable the production of sialylated glycoproteins in cells of interest. These carbohydrate engineering efforts encompass the implementation of new carbohydrate bioassays, the examination of a selection of insect cell lines and the use of bioinformatics to identify gene sequences for critical processing enzymes. The compositions comprise cells of interest producing sialylated glycoproteins. The methods and compositions are useful for heterologous expression of glycoproteins.