Patents by Inventor Shihshieh Huang

Shihshieh Huang has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Plant microRNAs and methods of use thereof
    Patent number: 8946511
    Abstract: This invention discloses novel microRNAs and their precursors, and recombinant DNA constructs including such novel miRNAs, miRNA precursors, miRNA promoters, and miRNA recognition sites corresponding to the miRNAs. Included are novel miRNA and miRNA precursors that exhibit nutrient-responsive expression. Also disclosed are miRNA decoy sequences. Further provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention and methods of controlling gene expression using recombinant DNA constructs of this invention.
    Type: Grant
    Filed: October 12, 2007
    Date of Patent: February 3, 2015
    Assignee: Monsanto Technology LLC
    Inventors: Edwards Allen, Barry S. Goldman, Liang Guo, Sara E. Heisel, Shihshieh Huang, Sergey I. Ivashuta, David K. Kovalik, Elysia K. Krieger, James K. Roberts, Yuanji Zhang
  • RECOMBINANT DNA CONSTRUCTS EMPLOYING SITE-SPECIFIC RECOMBINATION
    Publication number: 20140338010
    Abstract: Recombinant DNA constructs, for use in plants and plant cells, have site-specific recombination sites that allow assessing phenotypes and modes of action by over expression or suppression of endogenous genes. In an aspect, a single DNA construct can be switched between over expression and suppression by the action of a recombinase such as the Cre recombinase on constructs having lox recombination sites. Other useful recombination systems include the Flp/frt system, the R/Rs system, the Dre/rox system, and the GIN/gix system.
    Type: Application
    Filed: July 22, 2014
    Publication date: November 13, 2014
    Applicant: MONSANTO TECHNOLOGY LLC
    Inventors: Larry A. Gilbertson, Shihshieh Huang, Thomas Malvar
  • Methods and compositions for obtaining marker-free transgenic plants
    Patent number: 8829275
    Abstract: The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.
    Type: Grant
    Filed: July 25, 2012
    Date of Patent: September 9, 2014
    Assignee: Monsanto Technology LLC
    Inventors: Xudong Ye, Michael W. Petersen, Larry Gilbertson, David Walters, Susan Johnson, Shihshieh Huang, Paul S. Chomet
  • Recombinant DNA constructs employing site-specific recombination
    Patent number: 8816153
    Abstract: Recombinant DNA constructs, for use in plants and plant cells, have site-specific recombination sites that allow assessing phenotypes and modes of action by over expression or suppression of endogenous genes. In an aspect, a single DNA construct can be switched between over expression and suppression by the action of a recombinase such as the Cre recombinase on constructs having lox recombination sites. Other useful recombination systems include the Flp/frt system, the R/Rs system, the Dre/rox system, and the GIN/gix system.
    Type: Grant
    Filed: August 27, 2010
    Date of Patent: August 26, 2014
    Assignee: Monsanto Technology LLC
    Inventors: Larry A. Gilbertson, Shihshieh Huang, Thomas Malvar
  • ENHANCED ZEIN REDUCTION IN TRANSGENIC CORN SEED
    Publication number: 20140173778
    Abstract: Anti-sense-oriented RNA gene suppression agents in the form of a loop of anti-sense-oriented RNA is produced in cells of transgenic organisms, e.g. plants, by transcription from a recombinant DNA construct that comprises in 5? to 3? order a promoter element operably linked to more than one anti-sense-oriented DNA element and one or more complementary DNA elements.
    Type: Application
    Filed: June 10, 2013
    Publication date: June 19, 2014
    Applicant: Monsanto Technology LLC
    Inventors: Michael H. Luethy, Thomas M. Malvar, Shihshieh Huang
  • METHODS AND COMPOSITIONS FOR OBTAINING MARKER-FREE TRANSGENIC PLANTS
    Publication number: 20130198895
    Abstract: The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.
    Type: Application
    Filed: July 25, 2012
    Publication date: August 1, 2013
    Inventors: Xudong Ye, Michael W. Petersen, Larry Gilbertson, David Walters, Susan Johnson, Shihshieh Huang, Paul S. Chomet
  • Enhanced zein reduction in transgenic corn seed
    Patent number: 8461418
    Abstract: Anti-sense-oriented RNA gene suppression agents in the form of a loop of anti-sense-oriented RNA is produced in cells of transgenic organisms, e.g. plants, by transcription from a recombinant DNA construct that comprises in 5? to 3? order a promoter element operably linked to more than one anti-sense-oriented DNA element and one or more complementary DNA elements.
    Type: Grant
    Filed: November 24, 2008
    Date of Patent: June 11, 2013
    Assignee: Monsanto Technology LLC
    Inventors: Michael H. Luethy, Thomas M. Malvar, Shihshieh Huang
  • Multiple virus resistance in plants
    Patent number: 8455716
    Abstract: The present invention provides gene targets, constructs and methods for the genetic control of plant disease caused by multiple plant viruses. The present invention relates to achieving a plant protective effect through the identification of target coding sequences and the use of recombinant DNA technologies for post-transcriptionally repressing or inhibiting expression of the target coding sequences of plant-parasitic viruses. Protein-expression based approaches may also be utilized to augment phenotype resistance. Thus, transcription of a single transgenic event comprising one or more plant expression cassettes can allow for broad spectrum resistance of a plant to multiple plant viral strains and species among the geminiviruses, tospoviruses, and potexviruses.
    Type: Grant
    Filed: April 20, 2010
    Date of Patent: June 4, 2013
    Assignee: Monsanto Technology LLC
    Inventors: Shihshieh Huang, Stanislaw Flasinski, Alessandra Frizzi, Brad Gabor, Charles Hagen, John Kao, Raquel Salati
  • GENE SEQUENCES AND USES THEREOF IN PLANTS
    Publication number: 20130074202
    Abstract: This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.
    Type: Application
    Filed: May 14, 2012
    Publication date: March 21, 2013
    Applicant: Monsanto Technology LLC
    Inventors: Thomas Adams, Ameeta K. Agarwa, Jeffrey Ahrens, James A. Ball, Amarjit Basra, Erin Bell, Terry L. Bradshaw, Paul S. Chomet, James H. Crowley, Jill Deikman, Molian Deng, Meghan Donnarummo, Kimberly Faye Zobrist Duff, Stephen Duff, Michael D. Edgerton, Banu Gopalan, Xiaoping He, Mackenzie Heal, Brendan S. Hinchey, Shihshieh Huang, Richard G. Johnson, Vincent Jung, Keith Kretzmer, Lucille B. Laccetti, Chao Qiang Lai, Garrett J. Lee, Michael H. Luethy, Timothy J. Leland, Jingdong Liu, Bin Lu, Adrian A. Lund, Linda L. Madson, Kathleen P. Malloy, Marcus Paul McNabnay, Manchikanti Padmavati, Thomas Ruff, William Start, Daniel Tennessen, Carl P. Urwin, Gabriela Vaduva, K. R. Vidya, Steven Thomas Voss, Christine Voyles, Zhango Xin, Nanfei Xu, Qingzhang Xu, Qiang Zhang, Yajuan Zhao, Li Zhou
  • Recombinant DNA constructs and methods for controlling gene expression
    Patent number: 8395023
    Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
    Type: Grant
    Filed: July 8, 2010
    Date of Patent: March 12, 2013
    Assignee: Monsanto Technology LLC
    Inventors: Larry A. Gilbertson, Sara E. Heisel, Shihshieh Huang, Elysia K. Krieger
  • Methods for producing hybrid seed
    Patent number: 8334430
    Abstract: This invention provides methods for producing a non-natural hybrid seed. Also disclosed are specific miRNAs and miRNA recognition sites useful for conferring inducible sterility on a crop plant, and recombinant DNA construct including such exogenous miRNA recognition sites.
    Type: Grant
    Filed: September 20, 2006
    Date of Patent: December 18, 2012
    Assignee: Monsanto Technology LLC
    Inventors: Edwards M. Allen, Larry A. Gilbertson, Nancy M. Houmard, Shihshieh Huang, Sergey I. Ivashuta, James K. Roberts
  • Corn seed comprising novel opaque modifiers and related methods
    Patent number: 8299335
    Abstract: Novel corn opaque modifier loci, associated molecular markers and methods for obtaining the loci, markers, and resultant seed are provided. Vitreous seed with reduced alpha-zein storage protein content that contain the opaque modifier loci are also described. Processes for obtaining milled corn seed products from the vitreous seed with reduced alpha-zein storage protein content that contain the opaque modifier loci are also provided.
    Type: Grant
    Filed: March 30, 2009
    Date of Patent: October 30, 2012
    Assignee: Monsanto Technology LLC
    Inventors: Thomas Malvar, Shihshieh Huang, Alessandra Frizzi, Mike Kerns
  • Methods and compositions for obtaining marker-free transgenic plants
    Patent number: 8237016
    Abstract: The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.
    Type: Grant
    Filed: October 12, 2011
    Date of Patent: August 7, 2012
    Assignee: Monsanto Technology LLC
    Inventors: Xudong Ye, Michael W. Petersen, Larry Gilbertson, David Walters, Susan Johnson, Shihshieh Huang, Paul S. Chomet
  • Recombinant DNA constructs and methods for controlling gene expression
    Patent number: 8217227
    Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
    Type: Grant
    Filed: October 10, 2006
    Date of Patent: July 10, 2012
    Assignee: Monsanto Technology LLC
    Inventors: Edwards Allen, Larry A. Gilbertson, Shihshieh Huang, Thomas Malvar, James K. Roberts
  • METHODS AND COMPOSITIONS FOR OBTAINING MARKER-FREE TRANSGENIC PLANTS
    Publication number: 20120131699
    Abstract: The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.
    Type: Application
    Filed: October 12, 2011
    Publication date: May 24, 2012
    Inventors: Xudong Ye, Michael W. Petersen, Larry Gilbertson, David Walters, Susan Johnson, Shihshieh Huang, Paul S. Chomet
  • RECOMBINANT DNA CONSTRUCTS EMPLOYING SITE-SPECIFIC RECOMBINATION
    Publication number: 20120054884
    Abstract: Recombinant DNA constructs, for use in plants and plant cells, have site-specific recombination sites that allow assessing phenotypes and modes of action by over expression or suppression of endogenous genes. In an aspect, a single DNA construct can be switched between over expression and suppression by the action of a recombinase such as the Cre recombinase on constructs having lox recombination sites. Other useful recombination systems include the Flp/frt system, the R/Rs system, the Dre/rox system, and the GIN/gix system.
    Type: Application
    Filed: August 27, 2010
    Publication date: March 1, 2012
    Inventors: Larry A. Gilbertson, Shihshieh Huang, Thomas Malvar
  • Methods and compositions for obtaining marker-free transgenic plants
    Patent number: 8076536
    Abstract: The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.
    Type: Grant
    Filed: May 11, 2007
    Date of Patent: December 13, 2011
    Assignee: Monsanto Technology LLC
    Inventors: Xudong Ye, Michael W. Petersen, Larry Gilbertson, David Walters, Susan Johnson, Shihshieh Huang, Paul S. Chomet
  • GENE SEQUENCES AND USES THEREOF IN PLANTS
    Publication number: 20110258734
    Abstract: This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.
    Type: Application
    Filed: March 11, 2011
    Publication date: October 20, 2011
    Applicant: Monsanto Technology LLC
    Inventors: Thomas Adams, Ameeta K. Agarwa, Jeffrey Ahrens, James A. Ball, Amarjit Basra, Erin Bell, Terry L. Bradshaw, Paul S. Chomet, James H. Crowley, Jill Deikman, Molian Deng, Meghan Donnarummo, Kimberly Faye Zobrist Duff, Stephen Duff, Michael D. Edgerton, Banu Gopalan, Xiaoping He, Mackenzie Heal, Brendan S. Hinchey, Shihshieh Huang, Richard G. Johnson, Vincent Jung, Keith Kretzmer, Lucille B. Laccetti, Chao Qiang Lai, Garrett J. Lee, Michael H. Luethy, Timothy J. Leland, Jingdong Liu, Bin Lu, Adrian A. Lund, Linda L. Madson, Kathleen P. Malloy, Marcus Paul McNabnay, Manchikanti Padmavati, Thomas Ruff, William Start, Daniel Tennessen, Carl P. Urwin, Gabriela Vaduva, K.R. Vidya, Steven Thomas Voss, Christine Voyles, Zhango Xin, Nanfei Xu, Qingzhang Xu, Qiang Zhang, Yajuan Zhao, Li Zhou
  • Transgenic corn seed with enhanced free lysine
    Patent number: 7982105
    Abstract: Transgenic corn seed with high levels of free lysine resulting from a recombinant DNA construct having an endosperm specific promoter and an embryo specific promoter operably linked to gene suppression elements for suppressing the production of an endogenous lysine catabolite.
    Type: Grant
    Filed: August 31, 2006
    Date of Patent: July 19, 2011
    Assignee: Monsanto Technology LLC
    Inventors: Wayne E. Brown, Shihshieh Huang, Thomas M. Malvar
  • Methods for enhancing segregation of transgenes in plants
    Patent number: 7960610
    Abstract: The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independent transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.
    Type: Grant
    Filed: September 18, 2007
    Date of Patent: June 14, 2011
    Assignee: Monsanto Technology LLC
    Inventors: Toni Armstrong, Kenneth Barton, Laura J. Crow, Larry A. Gilbertson, Shihshieh Huang, Yong Huang, Brian J. Martinell, Michael W. Petersen, Kenneth J. Piller, Qi Wang, Xudong Ye