Patents by Inventor Shihshieh Huang

Shihshieh Huang has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Reversible male sterility in transgenic plants by expression of cytokinin oxidase
    Patent number: 7951997
    Abstract: Methods for the production of reversibly male-sterile plants by introduction of a polynucleotide encoding a cytokinin oxidase are disclosed along with nucleic acid constructs and transformed cells useful in the production of such plants. Also disclosed is the use of plants containing recombinant nucleic acid sequences in preventing pollination of plants with pollen containing one or more transgenes and in the introduction of economically important traits into elite varieties of plants.
    Type: Grant
    Filed: April 16, 2007
    Date of Patent: May 31, 2011
    Assignee: Monsanto Technology LLC
    Inventors: Shihshieh Huang, Lyle Dean Crossland, Roy O. Morris, Nordine Cheikh
  • RECOMBINANT DNA FOR GENE SUPPRESSION
    Publication number: 20110126321
    Abstract: Anti-sense-oriented RNA gene suppression agents in the form of a loop of anti-sense-oriented RNA is produced in cells of transgenic organisms, e.g. plants, by transcription from a recombinant DNA construct which comprises in 5? to 3? order a promoter element operably linked to an anti-sense-oriented DNA element and a complementary DNA element.
    Type: Application
    Filed: December 20, 2010
    Publication date: May 26, 2011
    Inventors: Shihshieh Huang, Thomas M. Malvar, Michael H. Luethy
  • TRANSGENIC PLANT SEED WITH INCREASED LYSINE
    Publication number: 20110113514
    Abstract: Disclosed herein are transgenic plants having a novel exogenous DNA construct that expresses a dihydrodipicolinic acid synthase and a gene suppression molecule for lysine ketoglutarate reductase, the activity of which results in increased lysine in a plant seed.
    Type: Application
    Filed: October 21, 2010
    Publication date: May 12, 2011
    Inventors: Thomas M. Malvar, Shihshieh Huang, Michael H. Luethy
  • Recombinant DNA Constructs and Methods for Controlling Gene Expression
    Publication number: 20110035838
    Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
    Type: Application
    Filed: July 8, 2010
    Publication date: February 10, 2011
    Inventors: Linda Lutfiyya, Liang Guo, Philip W. Miller, Michael H. Luethy, Sara E. Heisel, Yuanji Zhang, Edwards Allen, Larry A. Gilbertson, David K. Kovalic, James K. Roberts, Shihshieh Huang, Alessandra Frizzi, Nancy Houmard, Thomas Malvar
  • Recombinant DNA Constructs and Methods for Controlling Gene Expression
    Publication number: 20110035839
    Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
    Type: Application
    Filed: July 8, 2010
    Publication date: February 10, 2011
    Inventors: Linda Lutfiyya, Liang Guo, Philip W. Miller, Michael H. Leuthy, Sara E. Heisel, Yuanji Zhang, Edwards Allen, Larry A. Gilbertson, David K. Kovalic, James K. Roberts, Shihshieh Huang, Alessandra Frizzi, Nancy Houmard, Thomas Malvar
  • Compositions for enhancing segregation of transgenes in plants
    Patent number: 7858370
    Abstract: The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independent transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.
    Type: Grant
    Filed: September 18, 2007
    Date of Patent: December 28, 2010
    Assignee: Monsanto Technology LLC
    Inventors: Toni Armstrong, Kenneth Barton, Laura J. Crow, Larry A. Gilbertson, Shihshieh Huang, Yong Huang, Brian J. Martinell, Michael W. Petersen, Kenneth J. Piller, Qi Wang, Xudong Ye
  • Recombinant DNA for gene suppression
    Patent number: 7855323
    Abstract: Anti-sense-oriented RNA gene suppression agents in the form of a loop of anti-sense-oriented RNA is produced in cells of transgenic organisms, e.g. plants, by transcription from a recombinant DNA construct which comprises in 5? to 3? order a promoter element operably linked to an anti-sense-oriented DNA element and a complementary DNA element.
    Type: Grant
    Filed: February 10, 2005
    Date of Patent: December 21, 2010
    Assignee: Monsanto Technology LLC
    Inventors: Shihshieh Huang, Thomas M. Malvar, Michael H. Luethy
  • Multiple Virus Resistance in Plants
    Publication number: 20100269224
    Abstract: The present invention provides gene targets, constructs and methods for the genetic control of plant disease caused by multiple plant viruses. The present invention relates to achieving a plant protective effect through the identification of target coding sequences and the use of recombinant DNA technologies for post-transcriptionally repressing or inhibiting expression of the target coding sequences of plant-parasitic viruses. Protein-expression based approaches may also be utilized to augment phenotype resistance. Thus, transcription of a single transgenic event comprising one or more plant expression cassettes can allow for broad spectrum resistance of a plant to multiple plant viral strains and species among the geminiviruses, tospoviruses, and potexviruses.
    Type: Application
    Filed: April 20, 2010
    Publication date: October 21, 2010
    Inventors: Shihshieh Huang, Stanislaw Flasinski, Alessandra Frizzi, Brad Gabor, Charles Hagen, John Kao, Raquel Salati
  • Reversible Male Sterility in Transgenic Plants by Expression of Cytokinin Oxidase
    Publication number: 20100242132
    Abstract: Methods for the production of reversibly male-sterile plants by introduction of a polynucleotide encoding a cytokinin oxidase are disclosed along with nucleic acid constructs and transformed cells useful in the production of such plants. Also disclosed is the use of plants containing recombinant nucleic acid sequences in preventing pollination of plants with pollen containing one or more transgenes and in the introduction of economically important traits into elite varieties of plants.
    Type: Application
    Filed: April 16, 2007
    Publication date: September 23, 2010
    Applicant: MONSANTO TECHNOLOGY LLC
    Inventors: Shihshieh Huang, Lyle Dean Crossland, Nordine Cheikh, Roy O. Morris
  • Recombinant DNA Constructs and Methods for Controlling Gene Expression
    Publication number: 20100223694
    Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
    Type: Application
    Filed: March 18, 2010
    Publication date: September 2, 2010
    Inventors: Linda Lutfiyya, Liang Guo, Philip W. Miller, Michael H. Luethy, Sara E. Heisel, Yanji Zhang, Edwards Allen, Larry A. Gilbertson, David K. Kovalic, James K. Roberts, Shihshieh Huang, Alessandra Frizzi, Nancy Houmard, Thomas Malvar
  • Recombinant DNA constructs compressing engineered plant microRNAs and methods for controlling gene expression
    Patent number: 7786350
    Abstract: The present invention provides constructs containing engineered miRNAs or miRNA precursors derived from a maize miR408b, transgenic plant cells, plants, and seeds containing such constructs, and methods for their use.
    Type: Grant
    Filed: October 10, 2006
    Date of Patent: August 31, 2010
    Assignee: Monsanto Technology, LLC
    Inventors: Edwards Allen, Larry A. Gilbertson, Shihshieh Huang, Elysia K. Krieger
  • Recombinant DNA constructs and methods for controlling gene expression
    Patent number: 7786351
    Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
    Type: Grant
    Filed: October 10, 2006
    Date of Patent: August 31, 2010
    Assignee: Monsanto Technology, LLC
    Inventors: Nancy Houmard, Shihshieh Huang, Thomas Malvar
  • Engineered miRNA precursors for suppressing target gene expression in plants
    Patent number: 7723568
    Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
    Type: Grant
    Filed: October 10, 2006
    Date of Patent: May 25, 2010
    Assignee: Monsanto Technology LLC
    Inventors: Linda Lutfiyya, Liang Guo, Philip W. Miller, Michael H. Luethy, Sara E. Heisel, Yuanji Zhang, Edwards Allen, Larry A. Gilbertson, David K. Kovalic, James K. Roberts, Shihshieh Huang, Alessandra Frizzi, Nancy Houmard, Thomas Malvar
  • Maize seed with synergistically enhanced lysine content
    Patent number: 7683237
    Abstract: The present invention provides a transgenic maize plant having in its genome transgenic DNA including sequence for zein reduction and sequence for lysine biosynthesis, whereby expression of the transgenic DNA results in a synergistically increased lysine content of seed of the transgenic maize plant. The invention further provides a method for providing maize seed with synergistically increased lysine content.
    Type: Grant
    Filed: March 10, 2005
    Date of Patent: March 23, 2010
    Assignee: Monsanto Technology LLC
    Inventors: Alan L. Kriz, Shihshieh Huang, Michael H. Luethy
  • Corn Seed Comprising Novel Opaque Modifiers and Related Methods
    Publication number: 20090246350
    Abstract: Novel corn opaque modifier loci, associated molecular markers and methods for obtaining the loci, markers, and resultant seed are provided. Vitreous seed with reduced alpha-zein storage protein content that contain the opaque modifier loci are also described. Processes for obtaining milled corn seed products from the vitreous seed with reduced alpha-zein storage protein content that contain the opaque modifier loci are also provided.
    Type: Application
    Filed: March 30, 2009
    Publication date: October 1, 2009
    Applicant: MONSANTO TECHNOLOGY LLC
    Inventors: Thomas Malvar, Shihshieh Huang, Alessandra Frizzi, Mike Kerns
  • Methods for Producing Hybrid Seed
    Publication number: 20090235388
    Abstract: This invention provides methods for producing a non-natural hybrid seed. Also disclosed are specific miRNAs and miRNA recognition sites useful for conferring inducible sterility on a crop plant, and recombinant DNA construct including such exogenous miRNA recognition sites.
    Type: Application
    Filed: September 20, 2006
    Publication date: September 17, 2009
    Inventors: Edwards M. Allen, Larry A. Gilbertson, Nancy M. Houmard, Shihshieh Huang, Sergey I. Ivashuta, James K. Roberts
  • ENHANCED ZEIN REDUCTION IN TRANSGENIC CORN SEED
    Publication number: 20090158463
    Abstract: Anti-sense-oriented RNA gene suppression agents in the form of a loop of anti-sense-oriented RNA is produced in cells of transgenic organisms, e.g. plants, by transcription from a recombinant DNA construct that comprises in 5? to 3? order a promoter element operably linked to more than one anti-sense-oriented DNA element and one or more complementary DNA elements.
    Type: Application
    Filed: November 24, 2008
    Publication date: June 18, 2009
    Inventors: Michael H. Luethy, Thomas M. Malvar, Shihshieh Huang
  • Transgenic plants with enhanced agronomic traits
    Publication number: 20090100536
    Abstract: This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.
    Type: Application
    Filed: July 18, 2007
    Publication date: April 16, 2009
    Applicant: Monsanto Company
    Inventors: Thomas Adams, Ameeta K. Agarwa, Jeffrey Ahrens, James A. Ball, Amarjit Basra, Erin Bell, Terry L. Bradshaw, Paul S. Chomet, James H. Crowley, Jill Deikman, Molian Deng, Meghan Donnarummo, Kimberly Faye Zobrist Duff, Stephen Duff, Michael D. Edgerton, Banu Gopalan, Xiaoping He, Mackenzie Heal, Brendan S. Hinchey, Shihshieh Huang, Richard G. Johnson, Vincent Jung, Keith Kretzmer, Lucille B. Laccetti, Chao Qiang Lai, Garrett J. Lee, Michael H. Luethy, Timothy J. Leland, Jingdong Liu, Bin Lu, Adrian A. Lund, Linda L. Madson, Kathleen P. Malloy, Marcus Paul McNabnay, Manchikanti Padmavati, Thomas Ruff, William Start, Daniel Tennessen, Carl P. Urwin, Gabriela Vaduva, K.R. Vidya, Steven Thomas Voss, Christine Voyles, Zhango Xin, Nanfei Xu, Qingzhang Xu, Qiang Zhang, Yajuan Zhao, Li Zhou
  • Plant microRNAs and methods of use thereof
    Publication number: 20090070898
    Abstract: This invention discloses novel microRNAs and their precursors, and recombinant DNA constructs including such novel miRNAs, miRNA precursors, miRNA promoters, and miRNA recognition sites corresponding to the miRNAs. Included are novel miRNA and miRNA precursors that exhibit nutrient-responsive expression. Also disclosed are miRNA decoy sequences. Further provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention and methods of controlling gene expression using recombinant DNA constructs of this invention.
    Type: Application
    Filed: October 12, 2007
    Publication date: March 12, 2009
    Inventors: Edwards Allen, Barry S. Goldman, Liang Guo, Sara E. Heisel, Shihshieh Huang, Sergey I. Ivashuta, David K. Kovalik, Elysia K. Krieger, James K. Roberts, Yuanji Zhang
  • Recombinant DNA constructs and methods for controlling gene expression
    Publication number: 20090013434
    Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.
    Type: Application
    Filed: October 10, 2006
    Publication date: January 8, 2009
    Inventors: Shihshieh Huang, Thomas Malvar, Michael H. Luethy, Philip W. Miller, Larry A. Gilbertson, Edwards Allen, Sara E. Heisel, David K. Kovalic, James K. Roberts, Nancy Houmard, Alessandra Frizzi, Yuanji Zhang, Liang Guo, Linda Lutfiyya