Abstract: We disclose methods of sorting or separating mixtures of living cells (e.g., eukaryotic, prokaryotic, mammalian, pathogenic, bacterial, viral, etc.). We perform our methods by activating cell-selective photophoric labels, which photosensitize and chemically reduce a photosensitive metal compound to form metal grains, particles or crystals. The metal adheres to the cells and forms the basis for sorting or separating different cell types. Photophoric labels may include chemiluminescent agents such as peroxidase enzymes activated with peroxidase substrates capable of luminescence. Photosensitive metal compounds may be present in a light-sensitive matrix or emulsion containing photosensitizable metal compounds, which form metal grains, particles or crystals upon exposure to a developer solution. Developer solutions are formulated to substantially allow living cells to remain viable after exposure to the developing solution.

Abstract: The present invention relates to matrixes, arrays, systems and methods for analyzing biomolecules by their isoelectric point, optionally, in combination with a second dimension analysis. The assortment of matrixes, arrays and systems provided herein are useful for causing a biomolecule under the influence of an electrical field to accumulate into an IEF buffer that comprises a pH value that is the same as the isoelectric point of the biomolecule. The methods of this invention are useful for, e.g., research and diagnostic purposes.

Abstract: A method of generating a change in a cellular process of a target cell is disclosed. The method comprises contacting the target cell with a solid buffer, so as to alter an intracellular pH value in at least a portion of the cell, thereby generating the change in a cellular process of a target cell of a multicellular organism. The method may be used to kill either eukaryotic or prokaryotic cells. Pharmaceutical compositions and devices are also enclosed.

Abstract: A method of estimating a range of age of a subject, the method comprising: a) making at least one measurement of epidermal ridges of the subject; and b) estimating at least a range of age of the subject, using the measurements of the epidermal ridges, without using the measurements to identify the subject.

Abstract: The present invention relates to matrixes, arrays, systems and methods for analyzing biomolecules by their isoelectric point, optionally, in combination with a second dimension analysis. The assortment of matrixes, arrays and systems provided herein are useful for causing a biomolecule under the influence of an electrical field to accumulate into an IEF buffer that comprises a pH value that is the same as the isoelectric point of the biomolecule. The methods of this invention are useful for, e.g., research and diagnostic purposes.

Abstract: The present invention relates to methods and apparatus for promoting rapid formation of biomolecule crystals from a solution of biomolecules, preferably proteins, wherein the protein solution undergoes rapid concentration according to its isoelectric point in an electric field. Protein crystallization according to the methods of the present invention takes place within a period of hours or less.

Abstract: A system and method of mapping irregularities of hard tissue. The method includes repeatedly transmitting ultra-sonic energy towards the hard tissue at a first angle of incidence, registering the origin of transmission if an echo-reflection the hard tissue is received, defining the origin of transmission in six degrees of freedom, calculating position co-ordinates for a point causing the echo-reflection and changing the origin of transmission. The method further includes compiling position co-ordinates to generate a map of the irregularities in the surface of the hard tissue. The system includes at least one ultrasonic transducer, a central processing unit and a position locator and adjustment mechanism operably connectable and configured to accomplish the disclosed method.

Abstract: A system and method of mapping irregularities of hard tissue. The method includes repeatedly transmitting ultra-sonic energy towards the hard tissue at a first angle of incidence, registering the origin of transmission if an echo-reflection the hard tissue is received, defining the origin of transmission in six degrees of freedom, calculating position co-ordinates for a point causing the echo-reflection and changing the origin of transmission. The method further includes compiling position co-ordinates to generate a map of the irregularities in the surface of the hard tissue. The system includes at least one ultrasonic transducer, a central processing unit and a position locator and adjustment mechanism operably connectable and configured to accomplish the disclosed method.

Abstract: The present invention relates to matrixes, arrays, systems and methods for analyzing biomolecules by their isoelectric point, optionally, in combination with a second dimension analysis. The assortment of matrixes, arrays and systems provided herein are useful for causing a biomolecule under the influence of an electrical field to accumulate into an IEF buffer that comprises a pH value that is the same as the isoelectric point of the biomolecule. The methods of this invention are useful for, e.g., research and diagnostic purposes.

Abstract: A method and apparatus for achieving ultrasonic coronary thrombolysis. After intravenous injection of a thrombus-specific ultrasound contrast agent, an array of ultrasonic transducers arranged in a pad placed on the patient's chest transmits ultrasound signals into the patient, from a virtual source. Reflected signals are then analyzed to determine the ratio of the second (or hall) harmonic to first harmonic components in the signals. A high ratio indicates the presence of a thrombus in the field of interrogation Time-of-flight data for each of the receivers is used to calculate a spatial location of the thrombus, and a phased array ultrasonic transmission is then focused on the thrombus, so as to achieve sonoparation.

Abstract: Methods, devices, and systems for sorting and/or separating and/or purifying particles based on selective adherence or attachment of particles to metal grains formed in a light sensitive layer or substrate containing a photosensitizable metal compound by irradiation of the layer or substrate with light and development of the photosensitized substrate or layer.

Abstract: A method and apparatus for achieving ultrasonic coronary thrombolysis. After intravenous injection of a thrombus-specific ultrasound contrast agent, an array of ultrasoniec transducers arranged in a pad placed on the patient's chest transmits ultrasound signals into the patient, from a virtual source. Reflected signals are then analyzed to determine the ratio of the second (or halt) harmonic to first harmonic components in the signals. A high ratio indicates the presence of a thrombus in the field of interrogation. Time-of-flight data for each of the receivers is used to calculate a spatial location of the thrombus, and a phased array ultrasonic transmission is then focused on the thrombus, so as to achieve sonoparation.

Abstract: A method for phased-array ultrasonic transmission into a body wherein transmission from each element of the phased-array is implemented according to a firing order designed to generate a summated ultrasonic signal apparently originating from a virtual source outside of the body. The timing and order of firing of the phased-array elements is calculated from the relative proximities of each of the elements to the desired virtual source. The method allows for instantaneous ultrasonic localization of rapidly moving targets such as coronary thromboses.

Abstract: A dosimeter for an x-ray brachytherapy system permits in situ monitoring and control of radiation treatment via a miniaturized energy transducer within a human body. The dosimeter comprises a scintillating optical fiber having a distal end which is placed at the treatment site and a proximal end which is coupled to a dosimeter measurement unit. Utilizing energy supplied by an energy source, the miniaturized transducer generates x-ray photons. The scintillating optical fiber absorbs x-ray photons, converts the x-ray photons into light photons, and conveys the light photons to a dosimeter measurement unit. The light photons are converted into an electrical current which is representative of the intensity of the x-ray photons. The dosimeter measurement unit utilizes the electrical current to calculate and display the instantaneous and accumulated radiation dose, and radiation dose parameters are utilized to adjust energy levels, which are sent to the miniature energy transducer.

Abstract: A method is disclosed for moving, isolating and/or identifying particles in a sample by placing said sample in a spatially varying electrical field wherein the spatially varying electrical field is following a mathematical nonmonotonous function, selected from the group consisting of linear, hyperbolic, parabolic, parabolic functions or y˜xp/q and combinations thereof wherein p q means an integer. Also various devices are disclosed for performing the method.