Abstract: The present inventors succeeded in culturing thick three-dimensional muscle tissue in which the muscle fibers are oriented in the same direction by providing channels for transporting a culture medium inside a hydrogel containing skeletal myoblasts. The three-dimensional muscle tissue produced according to the invention is expected to have a steak-like texture. Additionally, in regenerative medicine applications, damage in extensive and thick muscle tissue is expected to be healed with a single treatment by using thick three-dimensional muscle tissue.

Abstract: A method of producing a 3D tissue composite, comprising: a preparation step in which a multiple number of sheet-shaped first structures containing first cells are prepared, wherein at least one of the multiple number of first structures holds a second structure containing second cells; a stacking step in which the multiple number of first structures are stacked to form a 3D composite; and a culturing step in which the 3D composite is cultured to form a 3D tissue composite containing first tissues formed from the first cells and second tissues formed from the second cells.

Abstract: The present invention provides a measuring device which includes: a first container and a second container that are arranged adjacent to each other; and a partition wall provided between the first and second containers, the partition wall having a through hole(s) for forming a lipid bilayer(s). In this measuring device, a gas flow path(s) having a hydrophobic surface is/are formed in at least one of the first and second containers; the gas flow path(s) has/have an inlet and an outlet; the inlet and the outlet each communicate with the exterior of the measuring device; the gas flow path(s) open(s) into the container in which it/they is/are formed; and a gas flowing in the gas flow path(s) contacts the droplet filled in the container.

Abstract: Provided is a culture method for a microorganism using a plate-like microchamber having a plurality of wells independent from each other on a front surface thereof. The culture method for the microorganism includes: mixing a liquid culture medium containing agarose having a gelation temperature of no less than 5° C. and no more than 20° C. and the microorganism to obtain a mixture; enclosing the mixture into the well with the front surface facing upward; and keeping the microchamber in which the mixture is enclosed in the well at a predetermined temperature range.

Abstract: An evaluation instrument is used in evaluating a compound produced by a cell. The evaluation instrument includes a holding space for holding a suspension containing the cell and a through hole that is formed at a bottom of the holding space. The through hole is closed with a substance that is impermeable to the cell and allows the compound to move therethrough.

Abstract: The present invention provides a production method for a three-dimensional muscle tissue including the steps of: preparing an approximately rectangular first cell module containing skeletal myoblasts in a hydrogel, and having a plurality of approximately rectangular holes parallel to each other, and an approximately rectangular second cell module containing skeletal myoblasts in a hydrogel, and having a plurality of approximately rectangular holes parallel to each other at positions different from those of the first cell module in a vertical direction; alternately stacking the prepared first cell module and the prepared second cell module to obtain a stack; subjecting the skeletal myoblasts contained in the obtained stack to proliferation culture; and inducing the proliferated skeletal myoblasts to differentiate into myotubes.

Abstract: A graft is provided having a shell part that comes into contact with a body fluid when the graft is transplanted into a living body; and a core part that is located inside the shell part and does not substantially come into contact with the body fluid, in which the core part contains a plurality of first hydrogel fibers containing cells, the shell part contains a second hydrogel functioning as a semipermeable membrane, and the graft has a size with an outer diameter of greater than 1.5 mm.

Abstract: An object of the present invention is to provide an artificial tissue perfusion device capable of analyzing the interaction between a vascular cell layer and a parenchymal cell layer with high accuracy. An artificial tissue perfusion device includes a co-culture system (C) in which a plurality of types of cell are cultured. The co-culture system has a tubular well part (10) having a culture space (11) inside; a base material (20) having a perfusion flow path (26) which extends in a predetermined direction and is perfused with a medium, and a holding part (23) which opens to the perfusion flow path and holds the well part attachably and detachably; and a gel membrane (30) having a form of a porous membrane and disposed at an end portion of the well part facing the perfusion flow path in a case where the well part is held by the holding part.

Abstract: Provided are an alginate gel fiber for antibody production wherein an antibody-producing cell (for example, an antibody-producing CHO cell) is comprised in a core layer, and an antibody production method using the alginate gel fiber. An antibody production method is thereby additionally provided.

Abstract: A heat insulating sheet member capable of enhancing a heat insulating performance of an exhaust gas introduction path by an easy operation, including such a path in a turbocharger. The heat insulating sheet member is a bendable member formed from an inorganic flexible material. The heat insulating sheet member includes a first region corresponding to an inlet of a bottom wall portion, a second region corresponding to at least a terminating end portion of the scroll portion, a third region provided between the first region and the second region and corresponding to a coupling wall portion, and a fourth region corresponding to an outer peripheral wall portion. The first region and the third region, the third region and the second region, and the first region and the fourth region are coupled to each other with the inorganic flexible material in a continuous state.

Abstract: An array includes a plurality of tubes disposed in contact with each other such that axial directions are parallel to each other; and a target substance disposed inside at least one of the plurality of tubes, in which the plurality of tubes are made of anionic hydrogel, and surfaces on which the plurality of tubes are in contact with each other are bonded with an adhesive including nanoparticles having surfaces coated with a cationic water-soluble polymer.

Abstract: [Problem] The present invention addresses the problem of providing: a novel fluorescent gel for glucose monitoring, whereby a concentration of glucose in a living body can be continuously measured and fibrosis of the gel can be suppressed even when the gel is implanted for a long period of time; and continuous glucose monitoring using the fluorescent gel. [Solution] A fluorescent gel for glucose monitoring, having a three-dimensional mesh structure in which a plurality of four-armed polymers having a polyethylene glycol skeleton are crosslinked at terminal ends thereof, wherein a fluorescent dye unit exhibiting a fluorescence response by bonding with glucose is immobilized in the gel structure by covalent bonding.

Abstract: The method for producing a three-dimensional artificial tissue is a method in which a three-dimensional artificial tissue extending in a predetermined direction is produced. The method includes: preparing a device including a culture tank having a culturing space surrounded by sidewalls, and a flow channel-forming member that penetrates through the sidewalls that face each other and is suspended in the culturing space along a predetermined direction; culturing cells in the culturing space and thereby forming a three-dimensional artificial tissue through which the flow channel-forming member penetrates; and removing the flow channel-forming member from the three-dimensional artificial tissue and thereby forming a perfusion flow channel that penetrates through the three-dimensional artificial tissue.

Abstract: The present invention relates to a hollow microfiber comprising (1) one or more cell-adhesive layers having a cell-adhesive hydrogel, (2) an outer shell layer having a high-strength hydrogel that covers the outer periphery of the cell-adhesive layer that is positioned farthest from the center axis among the one or more cell-adhesive layers, and (3) a cell layer that covers the inner periphery of the cell-adhesive layer that is positioned closest to the center axis among the one or more cell-adhesive layers. The present invention also relates to a method of manufacturing the hollow microfiber and a kit for carrying out the manufacturing method.

Abstract: Recombinant collagenases with a stable specific activity and enzyme agents for cell and tissue dissociation such a recombinant are provided. The recombinant collagenase is derived from Grimontia hollisae-derived collagenase is characterized by having, from the N terminus to the C terminus, a collagenase catalytic domain, a linker region sequence, and a prepeptidase C terminal domain, which Grimontia hollisae-derived recombinant collagenase does not comprise at least the prepeptidase C terminal domain. The obtained recombinant collagenase has a high and stable specific activity.

Abstract: The method for producing a three-dimensional artificial tissue is a method in which a three-dimensional artificial tissue extending in a predetermined direction is produced. The method includes: preparing a device including a culture tank having a culturing space surrounded by sidewalls, and a flow channel-forming member that penetrates through the sidewalls that face each other and is suspended in the culturing space along a predetermined direction; culturing cells in the culturing space and thereby forming a three-dimensional artificial tissue through which the flow channel-forming member penetrates; and removing the flow channel-forming member from the three-dimensional artificial tissue and thereby forming a perfusion flow channel that penetrates through the three-dimensional artificial tissue.

Abstract: Recombinant collagenases with a stable specific activity and enzyme agents for cell and tissue dissociation such a recombinant are provided. The recombinant collagenase is derived from Grimontia hollisae-derived collagenase is characterized by having, from the N terminus to the C terminus, a collagenase catalytic domain, a linker region sequence, and a prepeptidase C terminal domain, which Grimontia hollisae-derived recombinant collagenase does not comprise at least the prepeptidase C terminal domain. The obtained recombinant collagenase has a high and stable specific activity.

Abstract: The present invention relates to a hollow microfiber comprising (1) one or more cell-adhesive layers having a cell-adhesive hydrogel, (2) an outer shell layer having a high-strength hydrogel that covers the outer periphery of the cell-adhesive layer that is positioned farthest from the center axis among the one or more cell-adhesive layers, and (3) a cell layer that covers the inner periphery of the cell-adhesive layer that is positioned closest to the center axis among the one or more cell-adhesive layers. The present invention also relates to a method of manufacturing the hollow microfiber and a kit for carrying out the manufacturing method.

Abstract: A method for preparing a linearly extended supramolecular fiber or a plurality of linearly aligned supramolecular fibers, which comprises the step of allowing supramolecular monomers to be self-assembled in a microfluidic channel.

Abstract: Disclosed is a substrate (10A) for trapping a microorganism or cell (T), characterized by comprising a base (4) and having a space (2) into which a fluid (R) containing the microorganism or cell (T) is introduced and a microfine suction hole (1) through which the space (2) communicates with the outside of the base (4). The substrate is further characterized in that the space (2) has been formed in the base (4), and at least the portion of the base (4) which forms the microfine suction hole (1) is constituted of a single member.