Patents by Inventor Shunhai Wang

Shunhai Wang has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Online Native Mass Spectrometry Methods for Assaying Viral Particles
    Publication number: 20230010418
    Abstract: Methods for determining the relative abundance of viral capsid components in a sample of viral particles are disclosed. In embodiments, methods for determining the relative abundance of empty capsids, partially-full capsids and full capsids (e.g., containing a heterologous nucleic acid molecule) of adeno-associated virus are disclosed.
    Type: Application
    Filed: July 11, 2022
    Publication date: January 12, 2023
    Inventors: Victoria Cotham, Shunhai Wang, Ning Li
  • Anti-VEGF protein compositions and methods for producing the same
    Patent number: 11548932
    Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.
    Type: Grant
    Filed: June 15, 2022
    Date of Patent: January 10, 2023
    Assignee: Regeneron Pharmaceuticals, Inc.
    Inventors: Shunhai Wang, Ning Li, Shawn Lawrence, Amy Johnson, Meghan Casey, Jaimie Grapel, Hunter Chen, Amardeep Singh Bhupender Bhalla
  • COUPLING ISOELECTRIC FOCUSING-BASED FRACTIONATION WITH MASS SPECTROMETRY ANALYSIS
    Publication number: 20230002445
    Abstract: The present invention generally pertains to methods of characterizing charge variants of a protein of interest. In particular, the present invention pertains to the use of desalting size exclusion chromatography-reduced peptide mapping mass spectrometry to identify charge variants separated by capillary isoelectric focusing.
    Type: Application
    Filed: June 29, 2022
    Publication date: January 5, 2023
    Inventors: Tao Xing, Yuetian Yan, Shunhai Wang
  • Anti-VEGF protein compositions and methods for producing the same
    Patent number: 11542317
    Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for administering such compositions.
    Type: Grant
    Filed: June 16, 2022
    Date of Patent: January 3, 2023
    Assignee: Regeneron Pharmaceuticals, Inc.
    Inventors: Shunhai Wang, Ning Li, Hunter Chen, Amardeep Singh Bhupender Bhalla
  • Quantitation and identification of dimers in co-formulations
    Patent number: 11536725
    Abstract: Methods and system for identification of dimer species using online chromatography and electrospray ionization mass spectrometry are provided. Also provided are methods and system for quantitation of heterodimer species using immunoprecipitation and liquid chromatography-mass spectrometry.
    Type: Grant
    Filed: January 23, 2020
    Date of Patent: December 27, 2022
    Assignee: Regeneron Pharmaceuticals, Inc.
    Inventors: Yuetian Yan, Shunhai Wang
  • Methods for producing aflibercept in chemically defined media having reduced aflibercept variants
    Patent number: 11535663
    Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.
    Type: Grant
    Filed: July 22, 2021
    Date of Patent: December 27, 2022
    Assignee: Regeneron Pharmaceuticals, Inc.
    Inventors: Shawn Lawrence, Amy Johnson, Meghan Casey, Jaimie Mastrogiacomo, Shunhai Wang, Ning Li
  • ANTI-VEGF PROTEIN COMPOSITIONS AND METHODS FOR PRODUCING THE SAME
    Publication number: 20220396608
    Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for administering such compositions.
    Type: Application
    Filed: June 16, 2022
    Publication date: December 15, 2022
    Inventors: Shunhai Wang, Ning Li, Hunter Chen, Amardeep Singh Bhupender Bhalla
  • Hydrophobic interaction chromatography-coupled native mass spectrometry for antibody analysis
    Patent number: 11525833
    Abstract: The present invention provides rapid, sensitive high-throughput methods and systems for characterizing peptides or proteins using hydrophobic interaction chromatography-coupled native mass spectrometry to improve manufacturing process of biopharmaceutical products, such as identifying impurities during antibody purification, monitoring post-translational modification variants during production, or characterizing drug-to-antibody ratio of antibody-drug conjugates. The separation profiles of the peptides or proteins are generated and compared to identify or qualify the peptides or proteins.
    Type: Grant
    Filed: September 27, 2020
    Date of Patent: December 13, 2022
    Assignee: Regeneron Pharmaceuticals, Inc.
    Inventors: Yuetian Yan, Shunhai Wang
  • System and method for characterizing protein dimerization
    Patent number: 11525823
    Abstract: Systems and methods to characterize dimerization interfaces at the subdomain level of a protein are provided. An exemplary method includes digesting a protein dimer sample into subdomains, labeling the digested protein sample, isolating labeled dimeric and monomeric subdomain fragments, and peptide mapping the labeled sample to determine where the dimer fragments are labeled and where the dimer fragments are not labeled. Regions that show decreased labeling extents in the dimer fraction than that in the monomer fraction are likely involved or in close proximity to the dimerization interface.
    Type: Grant
    Filed: June 12, 2020
    Date of Patent: December 13, 2022
    Assignee: Regeneron Pharmaceuticals, Inc.
    Inventors: Shunhai Wang, Yuetian Yan
  • ANTI-VEGF PROTEIN COMPOSITIONS AND METHODS FOR PRODUCING THE SAME
    Publication number: 20220389081
    Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.
    Type: Application
    Filed: June 15, 2022
    Publication date: December 8, 2022
    Inventors: Shunhai Wang, Ning Li, Shawn Lawrence, Amy Johnson, Meghan Casey, Jamie Mastrogiacomo, Hunter Chen, Amardeep Singh Bhupender Bhalla
  • Anti-VEGF protein compositions and methods for producing the same
    Patent number: 11505593
    Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.
    Type: Grant
    Filed: February 8, 2022
    Date of Patent: November 22, 2022
    Assignee: Regeneron Pharmaceuticals, Inc.
    Inventors: Shunhai Wang, Ning Li, Hunter Chen, Amardeep Singh Bhupender Bhalla
  • Anti-VEGF protein compositions and methods for producing the same
    Patent number: 11505594
    Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.
    Type: Grant
    Filed: April 1, 2022
    Date of Patent: November 22, 2022
    Assignee: Regeneran Pharmaceuticals, Inc.
    Inventors: Andrew Tustian, Ankit Vartak, Thomas Daly, Erica Pyles, Nisha Palackal, Shunhai Wang, Ning Li, Matthew Franklin, Shawn Lawrence, Amy Johnson, Meghan Casey, Jaimie Grapel
  • DUAL-COLUMN LC-MS SYSTEM AND METHODS OF USE THEREOF
    Publication number: 20220357336
    Abstract: Methods for achieving complete sequence coverage of monoclonal antibodies by trypsin digestion and dual-column LC-MS system are provided. The disclosed method improves upon current techniques for standard peptide mapping.
    Type: Application
    Filed: July 25, 2022
    Publication date: November 10, 2022
    Inventor: Shunhai Wang
  • Method and system of identifying and quantifying antibody fragmentation
    Patent number: 11486864
    Abstract: Methods and system for identifying and quantifying antibody fragments and identifying the site of fragmentation on an antibody are provided herein.
    Type: Grant
    Filed: January 15, 2020
    Date of Patent: November 1, 2022
    Assignee: Regeneron Pharmaceuticals, Inc.
    Inventors: Yuetian Yan, Shunhai Wang
  • Anti-VEGF protein compositions and methods for producing the same
    Patent number: 11485770
    Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.
    Type: Grant
    Filed: January 13, 2022
    Date of Patent: November 1, 2022
    Assignee: Regeneran Pharmaceuticals, Inc.
    Inventors: Shunhai Wang, Ning Li, Hunter Chen, Amardeep Singh Bhupender Bhalla
  • Methods for producing aflibercept in chemically defined media having reduced aflibercept variants
    Patent number: 11472861
    Abstract: The present disclosure pertains to compositions comprising anti-VEGF proteins and methods for producing such compositions.
    Type: Grant
    Filed: December 21, 2021
    Date of Patent: October 18, 2022
    Assignee: Regeneron Pharmaceuticals, Inc.
    Inventors: Shawn Lawrence, Amy Johnson, Meghan Casey, Jaimie Mastrogiacomo, Shunhai Wang, Ning Li
  • Anti-VEGF protein compositions and methods for producing the same
    Patent number: 11459373
    Abstract: The present disclosure pertains to compositions comprising aflibercept and methods for producing such compositions in chemically defined media and using anion exchange chromatography to produce aflibercept with a specific glycosylation profile. In particular, these methods produce aflibercept with a glycosylation profile with specific ranges of % total fucosylated glycans, total sialylated glycans, mannose-5, and galactosylated glycans.
    Type: Grant
    Filed: October 13, 2021
    Date of Patent: October 4, 2022
    Assignee: Regeneron Pharmaceuticals, Inc.
    Inventors: Andrew Tustian, Ankit Vartak, Thomas Daly, Erica Pyles, Nisha Palackal, Shunhai Wang, Ning Li
  • Anti-VEGF protein compositions and methods for producing the same
    Patent number: 11459374
    Abstract: The present disclosure pertains to methods for preparing a prefilled syringe and methods for preparing an auto injector prefilled syringe comprising aflibercept and methods for producing such aflibercept in chemically defined media.
    Type: Grant
    Filed: February 8, 2022
    Date of Patent: October 4, 2022
    Assignee: Regeneron Pharmaceuticals, Inc.
    Inventors: Andrew Tustian, Ankit Vartak, Thomas Daly, Erica Pyles, Nisha Palackal, Shunhai Wang, Ning Li
  • Dual-column LC-MS system and methods of use thereof
    Patent number: 11435359
    Abstract: Methods for achieving complete sequence coverage of monoclonal antibodies by trypsin digestion and dual-column LC-MS system are provided. The disclosed method improves upon current techniques for standard peptide mapping.
    Type: Grant
    Filed: December 17, 2020
    Date of Patent: September 6, 2022
    Assignee: Regeneron Pharmaceuticals, Inc.
    Inventor: Shunhai Wang
  • GLUCURONYLATION AS A NEW ACIDIC POST-TRANSLATIONAL MODIFICATION ON THERAPEUTIC MONOCLONAL ANTIBODIES
    Publication number: 20220276260
    Abstract: Compositions and methods for identifying glucuronylated protein drug products are provided.
    Type: Application
    Filed: May 16, 2022
    Publication date: September 1, 2022
    Applicant: Regeneron Pharmaceuticals, Inc.
    Inventor: Shunhai Wang