Abstract: The invention provides method, system and storage medium for solving electric field physical quantity in an electrochemical model. The method includes selecting a negative or positive electrode region as a calculation region; selecting a solid or liquid phase current as an observed quantity, and a solid- and liquid-phase potentials as a costate variable; inserting nodes between two endpoints of the calculation region, and determining a target value of the observed quantity of each node; constructing N calculation units; sequentially completing shooting of each of the N calculation units until a convergent solution of the target shooting of the N-th calculation unit is obtained, and taking the convergent solution as a deterministic solution of the costate variable; obtaining the physical quantity of each spatial point at the present time according to the observed quantity of the starting point at the present time and the deterministic solution of the costate variable.

Abstract: The invention discloses method and system for target-based electric field decoupling for an electrochemical model.

Abstract: Provided herein are methods, systems, and compositions for efficient nucleic acid assembly with improved representation and distribution. Provided herein are methods, systems, and compositions for efficient nucleic acid assembly of nucleic acids encoding genes for use in various downstream processes.

Abstract: The invention provides an electrochemical model based method for early warning for a lithium battery, including establishing a three-dimensional electrochemical model for the lithium battery, and dividing the lithium battery into three portions respectively including a positive electrode, a negative electrode and a separator; performing spatial discretization on the three-dimensional electrochemical model according to a preset accuracy to establish the four-dimensional spatial coordinates of the lithium battery; obtaining a current lithium ion concentration in each position of the lithium battery by simulation based on the four-dimensional space coordinates, a historical lithium ion concentration and a historical diffusion coefficient of the lithium battery; and performing early warning for the lithium battery according to the current lithium ion concentration in each position.

Abstract: The invention discloses method and system for predicting working conditions of lithium batteries. The method includes performing a Fourier transform on a physicochemical state quantity distribution function of a solid-phase lithium battery to calculate a physicochemical state quantity distribution series function in a frequency domain and obtain a solid-phase physicochemical state quantity in the frequency domain according to the physicochemical state quantity distribution series function; performing a Laplace transform on a partial differential governing equation set of the solid-phase physicochemical state quantity to obtain a solid-phase ordinary differential equation set in a complex frequency domain and obtain an analytical solution in the time domain through an inverse Laplace transform; and calculating, according to the analytical solution, a predicted value of the working conditions of the solid-phase lithium battery at any location and at any time in the future.

Abstract: Disclosed herein are methods for the generation of highly accurate oligonucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a selective library of variants. The variant oligonucleic acid libraries described herein may designed for further processing by transcription or translation. The variant oligonucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of disease.

Abstract: An apparatus including a bidirectional power converter circuit. The power converter circuit includes a primary circuit side including a plurality of primary switches, an isolation transformer, and a secondary circuit side separated from the primary circuit side by the isolation transformer. The secondary circuit side includes an inductor, a rectifier circuit coupled to the inductor and configured to receive energy from the primary circuit side and provide energy to the primary circuit side, and a clamp circuit coupled to the inductor and configured to provide a reset voltage to the inductor that prevents inductor current runaway.

Abstract: Devices and methods for de novo synthesis of large and highly accurate libraries of oligonucleic acids are provided herein. Devices include structures having a main channel and microchannels, where the microchannels have a high surface area to volume ratio. Devices disclosed herein provide for de novo synthesis of oligonucleic acids having a low error rate.

Abstract: Provided herein are compositions, methods and systems relating to libraries of polynucleotides such that the libraries allow for accurate and efficient hybridization after binding to target sequences. Further provided herein are probes, blockers, additives, buffers, and methods that result in improved hybridization. Such compositions and methods are useful for improvement of Next Generation Sequencing applications, such as reducing off-target binding or reducing workflow times.

Abstract: An apparatus including a bidirectional power converter circuit. The power converter circuit includes a primary circuit side including a plurality of primary switches, an isolation transformer, and a secondary circuit side separated from the primary circuit side by the isolation transformer. The secondary circuit side includes an inductor, a rectifier circuit coupled to the inductor and configured to receive energy from the primary circuit side and provide energy to the primary circuit side, and a clamp circuit coupled to the inductor and configured to provide a reset voltage to the inductor that prevents inductor current runaway.

Abstract: De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.

Abstract: Devices and methods for de novo synthesis of large and highly accurate libraries of oligonucleic acids are provided herein. Devices include structures having a main channel and microchannels, where the microchannels have a high surface area to volume ratio. Devices disclosed herein provide for de novo synthesis of oligonucleic acids having a low error rate.

Abstract: A charging circuit can include: a first module having a plurality of power transistors, and being coupled between a first port and a reference ground; a second module having a plurality of power transistors, and being coupled between a second port and the reference ground; at least one inductor coupled between the first module and the second module; and where at least one of the first module and the second module forms a multi-level converter with the at least one inductor.

Abstract: Disclosed is a novel jet-propelled lift-increasing and stability-increasing amphibious aircraft and an application method thereof. An air intake fan connects and communicates with an air intake end of a pressurized air storage tank, an air outlet end of the pressurized air storage tank connects and communicates with a shunting pipeline, the shunting pipeline respectively connects and communicates with a plurality of air chambers, each connection of the plurality of air chambers and the shunting pipeline is provided with an adjusting valve, and the plurality of air chambers are distributed in a plurality of positions of the bottom of the aircraft, and are configured to jet air outwards. A navigation state sensing device is configured to detect navigation data of the aircraft and send the navigation data to an intelligent analysis device. The intelligent analysis device is configured to analyze the navigation data, obtain a control scheme and send to a jet control device.

Abstract: Provided herein are methods, systems, and compositions for assembly of covalently closed double stranded nucleic acids. Provided herein are methods, systems, and compositions for assembly covalently closed double stranded nucleic acids for use in various downstream processes.

Abstract: De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.

Abstract: The present disclosure relates to systems and techniques for developing APIs that utilize multiple pre-existing APIs. The present disclosure also relates to a user interface that allows for chaining APIs together as a function of multiple pre-existing APIs. The present disclosure also relates to security and authorization of a user to execute one or more APIs as part of an API chain.

Abstract: De novo synthesized large libraries of nucleic acids are provided herein with low error rates. Further, devices for the manufacturing of high-quality building blocks, such as oligonucleotides, are described herein. Longer nucleic acids can be synthesized in parallel using microfluidic assemblies. Further, methods herein allow for the fast construction of large libraries of long, high-quality genes. Devices for the manufacturing of large libraries of long and high-quality nucleic acids are further described herein.

Abstract: Provided herein are compositions and methods for identifying genomic variants. Further provided herein are standards useful for determining the analytical sensitivity and/or accuracy of instruments configured to measure nucleic acid variant frequencies.

Abstract: Provided herein are compositions, methods and systems relating to libraries of polynucleotides such that the libraries allow for accurate and efficient hybridization after binding to target sequences. Further provided herein are probes, blockers, additives, buffers, and methods that result in improved hybridization. Such compositions and methods are useful for improvement of Next Generation Sequencing applications, such as reducing off-target binding or reducing workflow times.