Patents by Inventor Soren Kjaerulff
Soren Kjaerulff has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9128097Abstract: The present invention provides simple, rapid methods and procedures for analyzing cells, hereunder quantitative and qualitative assessment of cells, such as viability. The present invention relates to the use of various optionally substituted reporter compounds particularly detectable upon their reaction with thiol-containing species present in higher concentrations in intact (e.g., living) cells than in non-intact (e.g., dead, stressed and apoptotic) cells. The present invention also relates to the use of various optionally substituted reporter compounds particularly detectable upon their reaction with species present in intact and/or non-intact cells. Moreover, the present invention relates to the use of measuring techniques and/or instruments coupled with the use of various optionally substituted reporter compounds. The invention further relates to compositions used in methods for analyzing cells, such as a composition comprising N-(7-dimethylamino-4-methyl-3-coumarinyl)-maleimide (DACM).Type: GrantFiled: July 13, 2009Date of Patent: September 8, 2015Assignee: ChemoMetec A/SInventors: Mette Elena Skindersø, Helle Frobøse Sørensen, Søren Kjærulff
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Patent number: 9102977Abstract: Disclosed is a fast and simple method for quantification of nucleic acid of biological cells as 2-step protocol. In the first step cells are treated with an acidic solution containing a non-ionic detergent and a fluorescent DNA specific label. In the second step the sample may be neutralized. Determining of the content of nucleic can be performed by fluorescence microscopy. The method may also be used for obtaining information of cell cycle analysis, ploidy determination, measurements of nucleotide incorporation and assays for proliferation, health, stress level, apoptosis, necrosis, or other state of conditions of cells. The invention also relates to a kit of parts comprising an acidic agent, a detergent, a labelling agent and optionally a neutralization agent.Type: GrantFiled: February 10, 2011Date of Patent: August 11, 2015Assignee: ChemoMetec A/SInventors: Søren Kjaerulff, Martin Glensbjerg
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Publication number: 20150132823Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased.Type: ApplicationFiled: January 21, 2015Publication date: May 14, 2015Inventors: Allan Svendsen, Soren Kjaerulff, Henrik Bisgaard-Frantzen, Carsten Andersen
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Publication number: 20150099284Abstract: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered stability, in particular at high temperatures and/or at low pH relative, and/or low Ca2+ to the parent alpha-amylase.Type: ApplicationFiled: December 16, 2014Publication date: April 9, 2015Inventors: Thomas Thisted, Soren Kjaerulff, Carsten Andersen, Claus Crone Fuglsang
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Publication number: 20150053872Abstract: According to an embodiment of the invention, an apparatus to detect fluorescence from a sample is disclosed. The apparatus comprises a sample plane onto which the sample is arranged, an excitation light unit including at least a light source to illuminate the sample, and a detection unit comprising at least a detector having at least 100,000 active detection elements to detect a fluorescence signal from the sample.Type: ApplicationFiled: August 29, 2014Publication date: February 26, 2015Inventors: Søren Kjaerulff, Mette Elena Skinderso, Helle Frobose Sorensen, Frans Ejner Ravn, Martin Glensbjerg
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Publication number: 20140322795Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.Type: ApplicationFiled: July 14, 2014Publication date: October 30, 2014Inventors: Carsten Andersen, Christel Thea Jorgensen, Henrik Bisgaard-Frantzen, Allan Svendsen, Soren Kjaerulff
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Patent number: 8860938Abstract: According to an embodiment of the invention, an apparatus to detect fluorescence from a sample is disclosed. The apparatus comprises a sample plane onto which the sample is arranged, an excitation light unit including at least a light source to illuminate the sample, and a detection unit comprising at least a detector having at least 100,000 active detection elements to detect a fluorescence signal from the sample.Type: GrantFiled: October 21, 2009Date of Patent: October 14, 2014Assignee: ChemoMetec A/SInventors: Søren Kjærulff, Mette Elena Skindersø, Helle Frobøse Sørensen, Frans Ejner Ravn, Martin Glensbjerg
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Patent number: 8859255Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.Type: GrantFiled: March 12, 2013Date of Patent: October 14, 2014Assignee: Novozymes A/SInventors: Carsten Andersen, Christel Thea Jorgensen, Henrik Bisgaard-Frantzen, Allan Svendsen, Soren Kjaerulff
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Publication number: 20140248685Abstract: The invention relates to a variant of a parent Termamyl-like ?-amylase, which exhibits an alteration in at least one of the following properties relative to said parent ?-amylase: i) improved pH stability at a pH from 8 to 10.5; and/or ii) improved Ca2+ stability at pH 8 to 10.5, and/or iii) increased specific activity at temperatures from 10 to 60° C.Type: ApplicationFiled: January 24, 2014Publication date: September 4, 2014Applicant: NOVOZYMES A/SInventors: Torben Vedel Borchert, Allan Svendsen, Carsten Andersen, Bjarne Nielsen, Torben Lauesgaard Nissen, Soren Kjaerulff
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Patent number: 8697390Abstract: The present invention provides simple, rapid methods and procedures for analyzing cells, hereunder quantitative and qualitative assessment of cells. The present invention relates to the use of N-(9-acridinyl)maleimide (NAM) or to the use of 7-diethylamino-3-((4?-(iodoacetyl)amino)phenyl)-4-methylcoumarin (CPI), particularly detectable upon its reaction with species (e.g., sulphur-containing species) present in higher concentrations in intact (e.g., living) cells than in non-intact (e.g., dead) cells. The present invention also relates to the use of NAM or to the use of 7-diethylamino-3-((4?-(iodoacetyl)amino)phenyl)-4-methylcoumarin (CPI), particularly detectable upon its reaction with species present in intact and/or non-intact cells. Moreover, the present invention relates to the use of measuring techniques and/or instruments coupled with the use of NAM or with the use of 7-diethylamino-3-((4?-(iodoacetyl)amino)phenyl)-4-methylcoumarin (CPI).Type: GrantFiled: July 14, 2009Date of Patent: April 15, 2014Assignee: ChemoMetec A/SInventors: Mette Elena Skindersø, Helle Frobøse Sørensen, Søren Kjaerulff
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Publication number: 20140017715Abstract: The invention relates to a variant of a parent Termamyl-like ?-amylase, which exhibits an alteration in at least one of the following properties relative to said parent ?-amylase: i) improved pH stability at a pH from 8 to 10.5; and/or ii) improved Ca2+ stability at pH 8 to 10.5, and/or iii) increased specific activity at temperatures from 10 to 60° C.Type: ApplicationFiled: March 5, 2013Publication date: January 16, 2014Applicant: NOVOZYMES A/SInventors: Torben Vedel Borchert, Allan Svendsen, Carsten Andersen, Bjarne Nielsen, Torben Lauesgaard Nissen, Soren Kjaerulff
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Publication number: 20130252850Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased.Type: ApplicationFiled: May 20, 2013Publication date: September 26, 2013Applicant: NOVOZYMES A/SInventors: Allan Svendsen, Soren Kjaerulff, Henrik Bisgaard-Frantzen, Carsten Andersen
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Patent number: 8465957Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased.Type: GrantFiled: May 23, 2012Date of Patent: June 18, 2013Assignee: Novozymes A/SInventors: Allan Svendsen, Soren Kjaerulff, Henrik Bisgaard-Frantzen, Carsten Andersen
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Publication number: 20130129181Abstract: The invention relates to image analysis of dark field images obtained at low magnification below 10:1. Image analysis of dark field images obtained at low magnification can be combined with analyses of images obtained in respect of the same section of a sample and same magnification but with other techniques such as fluorescent microscopy. The system and method can be used e.g. for particle counting, particle size measurement, particle size distribution, morphology measurement, where the particles can be cells and/or cell parts. The invention also relates to a compact dark field light source unit, a system or apparatus including a microscope which by itself is compact and comprises the mentioned dark field light source unit.Type: ApplicationFiled: May 18, 2011Publication date: May 23, 2013Applicant: CHEMOMETEC A/SInventors: Martin Glensbjerg, Johan Christer Holm, Frans Ejner Ravn, Søren Kjaerulff
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Patent number: 8420370Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.Type: GrantFiled: January 20, 2012Date of Patent: April 16, 2013Assignee: Novozymes A/SInventors: Carsten Andersen, Christel Thea Jorgensen, Henrik Bisgaard-Frantzen, Allan Svendsen, Soren Kjaerulff
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Patent number: 8411476Abstract: An apparatus and a method for converting power from a power input to an DC output voltage or current, which apparatus has a serial resonance converter, where a first feedback circuit is connected from the output terminal to an error amplifier, where the apparatus further has a second feedback circuit with at least one first resistor that is connected to a coil and to ground, which second feed back circuit connects the line between the first resistor and the coil and towards an inverting integrator, the output of which is connected through a second capacitor to a second input at a control circuit. As a result, the oscillating frequency is under influence of a signal that depends on the voltage generated in the resistor connected in serial to the coil or transformer.Type: GrantFiled: February 2, 2010Date of Patent: April 2, 2013Assignee: Bang & Olufsen A/SInventor: Søren Kjaerulff Christensen
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Publication number: 20130045485Abstract: Disclosed is a fast and simple method for quantification of nucleic acid of biological cells as 2-step protocol. In the first step cells are treated with an acidic solution containing a non-ionic detergent and a fluorescent DNA specific label. In the second step the sample may be neutralised. Determining of the content of nucleic can be performed by fluorescence microscopy. The method may also be used for obtaining information of cell cycle analysis, ploidy determination, measurements of nucleotide incorporation and assays for proliferation, health, stress level, apoptosis, necrosis, or other state of conditions of cells. The invention also relates to a kit of parts comprising an acidic agent, a detergent, a labelling agent and optionally a neutralization agent.Type: ApplicationFiled: February 10, 2011Publication date: February 21, 2013Applicant: CHEMOMETEC A/SInventors: Søren Kjaerulff, Martin Glensbjerg
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Publication number: 20120122754Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.Type: ApplicationFiled: January 20, 2012Publication date: May 17, 2012Applicant: NOVOZYMES A/SInventors: Carsten Andersen, Christel Thea Jørgensen, Henrik Bisgård-Frantzen, Allan Svendsen, Søren Kjærulff
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Publication number: 20120083426Abstract: A method is described for releasing a soluble or membrane associated intracellular protein of interest (POI) comprising the steps of: providing a cell comprising a soluble or membrane associated intracellular POI; contacting the cell with a membrane extracting composition; and causing the POI to be released from the cell under conditions sufficient for the specific release of the POI and in a soluble form.Type: ApplicationFiled: July 21, 2011Publication date: April 5, 2012Inventors: Claus Lindvald JOHANSEN, Soren Kjaerulff, Susan Mampusta Madrid, Henrik Pedersen, Charlotte Horsmans Poulsen, Masoud Rajabi Zargahi
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Patent number: 8124395Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.Type: GrantFiled: November 23, 2010Date of Patent: February 28, 2012Assignee: Novozymes A/SInventors: Carsten Andersen, Christel Thea Jorgensen, Henrik Bisgard-Frantzen, Allan Svendsen, Soren Kjaerulff