Patents by Inventor Spiros Kambourakis
Spiros Kambourakis has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20210189002Abstract: The invention provides a recombinant Thraustochytriaceae cell for the production of a glycomolecule. The cell comprises a nucleic acid encoding a heterologous glycomolecule, and a sequence encoding a heterologous oligosaccharyltransferase. The cell produces the heterologous glycomolecule having a higher glycan occupancy compared to the same heterologous glycomolecule produced by a corresponding cell not comprising the heterologous oligosaccharyltransferase. The glycan occupancy can be more than 25%. The cells advantageously produce and, optionally secrete, the heterologous glycomolecule. Thus, the invention provides recombinant organisms that provide glycomolecules having a glycosylation profile that is more similar to the glycosylation profile produced in a manunalian cell.Type: ApplicationFiled: October 30, 2020Publication date: June 24, 2021Applicant: Conagen Inc.Inventors: Nicky C. Caiazza, Jun Urano, Spiros Kambourakis
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Publication number: 20210087540Abstract: The invention provides a recombinant Labyrinthulomycetes cell for the production of a low sulfate glycomolecule. The cell comprises a nucleic acid encoding a heterologous glycomolecule, and a sequence encoding a heterologous oligosaccharyltransferase. The cell produces the heterologous glycomolecule having fewer sulfated glycans compared to the same heterologous glycomolecule produced by a corresponding cell not comprising the heterologous oligosaccharyltransferase. The cells advantageously produce and, optionally secrete, the heterologous glycomolecule. Thus, the invention provides recombinant organisms that provide glycomolecules having a glycosylation profile that is more similar to the glycosylation profile produced in mammalian cell.Type: ApplicationFiled: October 30, 2020Publication date: March 25, 2021Inventors: Nicky C. Caiazza, Jun Urano, Spiros Kambourakis
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Patent number: 10428092Abstract: The present invention generally relates to processes for the enzymatic production of a phosphinothricin product or precursor thereof from a nitrile-containing substrate.Type: GrantFiled: May 15, 2017Date of Patent: October 1, 2019Assignee: Strategic Enzyme Applications, Inc.Inventors: Kim F. Albizati, Spiros Kambourakis
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Patent number: 10087161Abstract: The present invention provides methods of method of synthesizing 2,5-furan dicarboxylic acid (FDCA) and FDCA precursor molecules. The methods involve performing a chemical dehydration reaction on a gluconic acid derivative in the presence of a dehydration catalyst. In some embodiments the gluconic acid derivative can be 2-dehydro-3-deoxy gluconic acid (DHG) or an ester thereof, 2-ketogluconic acid (2KGA) or an ester thereof, and 5-ketogluconic acid (5KGA) or an ester thereof. The 2,5-furan dicarboxylic acid precursor molecule is thereby synthesized, which can be converted into FDCA. The chemical dehydration can be performed by a variety of acid basic catalysts.Type: GrantFiled: July 5, 2016Date of Patent: October 2, 2018Assignee: Synthetic Genomics, Inc.Inventors: Spiros Kambourakis, Benjamin M. Griffin
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Patent number: 9909154Abstract: The present invention provides methods of producing dicarboxylic acids. The methods involve incubating a fatty acid or hydrocarbon substrate with an enzyme to produce a dicarboxylic acid product. The enzyme acts on the substrate to produce a product that has been both over-oxidized and has undergone cleavage of a C—C bond. In some embodiments the enzymes having these useful characteristics are mutants of a cytochrome P450 enzyme, for example an enzyme of the class CYP102 or a mutant thereof. The invention provides enzymes where these desirable characteristics can be found in a single enzyme, and thus in some embodiments the methods can be performed through the action of a single enzyme.Type: GrantFiled: November 20, 2014Date of Patent: March 6, 2018Assignee: Synthetic Genomics, Inc.Inventors: Benjamin M. Griffin, Spiros Kambourakis
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Publication number: 20170247399Abstract: The present invention generally relates to processes for the enzymatic production of a phosphinothricin product or precursor thereof from a nitrile-containing substrate.Type: ApplicationFiled: May 15, 2017Publication date: August 31, 2017Applicant: Strategic Enzyme Applications, Inc.Inventors: Kim F. Albizati, Spiros Kambourakis, Alan Grubbs, Bennett C. Borer
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Patent number: 9683001Abstract: The present invention generally relates to processes for the enzymatic production of a phosphinothricin product or precursor thereof from a nitrile-containing substrate.Type: GrantFiled: March 4, 2015Date of Patent: June 20, 2017Assignee: Stragegic Enzyme Applications, Inc.Inventors: Kim F. Albizati, Spiros Kambourakis, Alan Grubbs, Bennett C. Borer
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Publication number: 20170088865Abstract: The present invention provides methods for producing a product of one or more enzymatic pathways. The pathways used in the methods of the invention involve one or more conversion steps such as, for example, an enzymatic conversion of guluronic acid into D-glucarate (Step 7); an enzymatic conversion of 5-ketogluconate (5-KGA) into L-Iduronic acid (Step 15); an enzymatic conversion of L-Iduronic acid into Idaric acid Step 7b); and an enzymatic conversion of 5-ketocluconate into 4,6-dihydroxy 2,5-diketo hexanoate (2,5-DDH) (Step 16). In some embodiments the methods of the invention produce 2,5-furandicarboxylic acid (FDCA) as a product. The methods include both enzymatic and chemical conversions as steps. Various pathways are also provided for converting glucose into 5-dehdyro-4-deoxy-glucarate (DDG), and for converting glucose into 2,5-furandicarboxylic acid (FDCA).Type: ApplicationFiled: December 13, 2016Publication date: March 30, 2017Inventors: Spiros Kambourakis, Benjamin M. Griffin, Kevin V. Martin
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Publication number: 20170050944Abstract: The present invention provides methods of method of synthesizing 2,5-furan dicarboxylic acid (FDCA) and FDCA precursor molecules. The methods involve performing a chemical dehydration reaction on a gluconic acid derivative in the presence of a dehydration catalyst. In some embodiments the gluconic acid derivative can be 2-dehydro-3-deoxy gluconic acid (DHG) or an ester thereof, 2-ketogluconic acid (2KGA) or an ester thereof, and 5-ketogluconic acid (5KGA) or an ester thereof. The 2,5-furan dicarboxylic acid precursor molecule is thereby synthesized, which can be converted into FDCA. The chemical dehydration can be performed by a variety of acid basic catalysts.Type: ApplicationFiled: July 5, 2016Publication date: February 23, 2017Inventors: Spiros Kambourakis, Benjamin M. Griffin
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Patent number: 9528133Abstract: The present invention provides methods for producing a product of one or more enzymatic pathways. The pathways used in the methods of the invention involve one or more conversion steps such as, for example, an enzymatic conversion of guluronic acid into D-glucarate (Step 7); an enzymatic conversion of 5-ketogluconate (5-KGA) into L-Iduronic acid (Step 15); an enzymatic conversion of L-Iduronic acid into Idaric acid Step 7b); and an enzymatic conversion of 5-ketocluconate into 4,6-dihydroxy 2,5-diketo hexanoate (2,5-DDH) (Step 16). In some embodiments the methods of the invention produce 2,5-furandicarboxylic acid (FDCA) as a product. The methods include both enzymatic and chemical conversions as steps. Various pathways are also provided for converting glucose into 5-dehdyro-4-deoxy-glucarate (DDG), and for converting glucose into 2,5-furandicarboxylic acid (FDCA). The methods also involve the use of engineered enzymes that perform reactions with high specificity and efficiency.Type: GrantFiled: March 21, 2014Date of Patent: December 27, 2016Assignee: Synthetic Genomics, Inc.Inventors: Spiros Kambourakis, Benjamin M. Griffin, Kevin V. Martin
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Patent number: 9506090Abstract: The present invention provides methods for producing a product of one or more enzymatic pathways. The pathways used in the methods of the invention involve one or more conversion steps such as, for example, an enzymatic conversion of guluronic acid into D-glucarate (Step 7); an enzymatic conversion of 5-ketogluconate (5-KGA) into L-Iduronic acid (Step 15); an enzymatic conversion of L-Iduronic acid into Idaric acid Step 7b); and an enzymatic conversion of 5-ketocluconate into 4,6-dihydroxy 2,5-diketo hexanoate (2,5-DDH) (Step 16). In some embodiments the methods of the invention produce 2,5-furandicarboxylic acid (FDCA) as a product. The methods include both enzymatic and chemical conversions as steps. Various pathways are also provided for converting glucose into 5-dehdyro-4-deoxy-glucarate (DDG), and for converting glucose into 2,5-furandicarboxylic acid (FDCA). The methods also involve the use of engineered enzymes that perform reactions with high specificity and efficiency.Type: GrantFiled: September 20, 2013Date of Patent: November 29, 2016Assignee: Synthetic Genomics, Inc.Inventors: Spiros Kambourakis, Benjamin M. Griffin, Kevin V. Martin
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Publication number: 20150239917Abstract: The present invention generally relates to processes for the enzymatic production of a phosphinothricin product or precursor thereof from a nitrile-containing substrate.Type: ApplicationFiled: March 4, 2015Publication date: August 27, 2015Applicant: STRATEGIC ENZYME APPLICATIONS, INC.Inventors: Kim F. Albizati, Spiros Kambourakis, Alan Grubbs, Bennett C. Borer
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Publication number: 20150176041Abstract: The present invention provides methods of producing dicarboxylic acids. The methods involve incubating a fatty acid or hydrocarbon substrate with an enzyme to produce a dicarboxylic acid product. The enzyme acts on the substrate to produce a product that has been both over-oxidized and has undergone cleavage of a C—C bond. In some embodiments the enzymes having these useful characteristics are mutants of a cytochrome P450 enzyme, for example an enzyme of the class CYP102 or a mutant thereof. The invention provides enzymes where these desirable characteristics can be found in a single enzyme, and thus in some embodiments the methods can be performed through the action of a single enzyme.Type: ApplicationFiled: November 20, 2014Publication date: June 25, 2015Inventors: Benjamin M. Griffin, Spiros Kambourakis
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Patent number: 8981142Abstract: The present invention generally relates to processes for the enzymatic production of a phosphinothricin product or precursor thereof from a nitrile-containing substrate.Type: GrantFiled: April 14, 2010Date of Patent: March 17, 2015Assignee: Strategic Enzyme Applications, Inc.Inventors: Kim F. Albizati, Spiros Kambourakis, Alan Grubbs, Bennett C. Borer
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Publication number: 20140206047Abstract: The present invention provides methods for producing a product of one or more enzymatic pathways. The pathways used in the methods of the invention involve one or more conversion steps such as, for example, an enzymatic conversion of guluronic acid into D-glucarate (Step 7); an enzymatic conversion of 5-ketogluconate (5-KGA) into L-Iduronic acid (Step 15); an enzymatic conversion of L-Iduronic acid into Idaric acid Step 7b); and an enzymatic conversion of 5-ketocluconate into 4,6-dihydroxy 2,5-diketo hexanoate (2,5-DDH) (Step 16). In some embodiments the methods of the invention produce 2,5-furandicarboxylic acid (FDCA) as a product. The methods include both enzymatic and chemical conversions as steps. Various pathways are also provided for converting glucose into 5-dehdyro-4-deoxy-glucarate (DDG), and for converting glucose into 2,5-furandicarboxylic acid (FDCA). The methods also involve the use of engineered enzymes that perform reactions with high specificity and efficiency.Type: ApplicationFiled: March 21, 2014Publication date: July 24, 2014Applicant: Synthetic Genomics, Inc.Inventors: Spiros Kambourakis, Benjamin M. Griffin, Kevin V. Martin
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Publication number: 20140106414Abstract: The present invention provides methods for producing a product of one or more enzymatic pathways. The pathways used in the methods of the invention involve one or more conversion steps such as, for example, an enzymatic conversion of guluronic acid into D-glucarate (Step 7); an enzymatic conversion of 5-ketogluconate (5-KGA) into L-Iduronic acid (Step 15); an enzymatic conversion of L-Iduronic acid into Idaric acid Step 7b); and an enzymatic conversion of 5-ketocluconate into 4,6-dihydroxy 2,5-diketo hexanoate (2,5-DDH) (Step 16). In some embodiments the methods of the invention produce 2,5-furandicarboxylic acid (FDCA) as a product. The methods include both enzymatic and chemical conversions as steps. Various pathways are also provided for converting glucose into 5-dehdyro-4-deoxy-glucarate (DDG), and for converting glucose into 2,5-furandicarboxylic acid (FDCA). The methods also involve the use of engineered enzymes that perform reactions with high specificity and efficiency.Type: ApplicationFiled: September 20, 2013Publication date: April 17, 2014Applicant: SYNTHETIC GENOMICS, INC.Inventors: Spiros KAMBOURAKIS, Kevin V. MARTIN
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Patent number: 8614081Abstract: The present disclosure relates to polypeptides having nitrilase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.Type: GrantFiled: July 22, 2010Date of Patent: December 24, 2013Assignee: Codexis, Inc.Inventors: Anke Krebber, Emily Mundorff, Marissa Mock, Spiros Kambourakis
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Publication number: 20130204031Abstract: The present invention generally relates to processes for the enzymatic production of a phosphinothricin product or precursor thereof from a nitrile-containing substrate.Type: ApplicationFiled: April 14, 2010Publication date: August 8, 2013Applicant: STRATEGIC ENZYME APPLICATIONS, INC.Inventors: Kim F. Albizati, Spiros Kambourakis, Alan Grubbs, Bennett C. Borer
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Publication number: 20120142063Abstract: The present disclosure relates to polypeptides having nitrilase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.Type: ApplicationFiled: July 22, 2010Publication date: June 7, 2012Applicant: Codexis ,Inc.Inventors: Anke Krebber, Emily Mundoff, Marissa Mock, Spiros Kambourakis
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Publication number: 20090298147Abstract: (+)-Sitophilure, the aggregation pheromone of the pests rice weevil and maize weevil, is synthesized in high yield and diastereomeric excess by contacting 4-methyl-3,5heptadione with a reduced nicotinamide cofactor and a ketoreductase enzyme capable of catalyzing the reduction of 4-methyl-3,5-heptadione to produce (4R,5S)-5-hydroxy-4-methyl-3-heptanone to the substantial exclusion of other diastereomers.Type: ApplicationFiled: December 22, 2006Publication date: December 3, 2009Inventors: Dimitris Kalaitzakis, J. David Rozzeli, Spiros Kambourakis, Ioulia Smonou