Patents by Inventor Stephan Freyer
Stephan Freyer has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 12146171Abstract: The present invention relates to a method for recovering a protein of interest from a fermentation broth by adding a salt of a divalent cation and increasing the pH.Type: GrantFiled: June 9, 2020Date of Patent: November 19, 2024Assignee: BASF SEInventors: Katrin Treier-Marxen, Sebastian Schoof, Andreas Schaedler, Stephan Freyer, Michael Helmut Kopf
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Publication number: 20220275355Abstract: The present invention relates to a method for recovering a protein of interest from a fermentation broth by adding a salt of a divalent cation and increasing the pH.Type: ApplicationFiled: June 9, 2020Publication date: September 1, 2022Inventors: Katrin Treier-Marxen, Sebastian Schoof, Andreas Schaedler, Stephan Freyer, Michael Helmut Kopf
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Publication number: 20220186234Abstract: The present invention is directed to an industrial fermentation process for cultivating a Bacillus cell in a chemically defined fermentation medium and a method for producing a protein of interest comprising the steps of providing a chemically defined fermentation medium, inoculating the fermentation medium with a Bacillus cell comprising a gene encoding a protein of interest, cultivating the Bacillus cell in the fermentation medium under conditions conductive for the growth of the Bacillus cell and the expression of the protein of interest, wherein the cultivation of the Bacillus cell comprises the addition of one or more feed solutions comprising one or more chemically defined carbon sources and one or more trace element ions to the fermentation medium.Type: ApplicationFiled: February 18, 2020Publication date: June 16, 2022Inventors: Andreas Daub, Aydin Golabgir Anbarani, Tobias Klein, Stephan Freyer, Thomas Kaeding, Max Fabian Felle, Paul Igor Costea
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Publication number: 20220186177Abstract: The present invention is directed to an industrial fermentation process for cultivating a Bacillus cell in a chemically defined fermentation medium and a method for producing a protein of inter-est comprising the steps of providing a chemically defined fermentation medium, inoculating the fermentation medium with a Bacillus cell comprising a gene encoding a protein of interest, cultivating the Bacillus cell in the fermentation medium under conditions conductive for the growth of the Bacillus cell and the expression of the protein of interest, wherein the cultivation of the Bacillus cell comprises the addition of one or more feed solutions comprising one or more chemically defined carbon sources and magnesium ions to the fermentation medium.Type: ApplicationFiled: February 18, 2020Publication date: June 16, 2022Inventors: Andreas Daub, Aydin Golabgir Anbarani, Tobias Klein, Stephan Freyer, Thomas Kaeding, Max Fabian Felle, Paul Igor Costea
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Patent number: 10655152Abstract: The present invention relates to means and methods for producing an amide compound from a nitrile compound with less acrylic acid as by-product using a Nitrile hydratase (NHase) and Amidase producing microorganism as biocatalyst. Also provided is an aqueous amide compound obtained by the methods of the invention as well as a composition comprising acrylamide or polyacrylamide as well as a dried microorganism exhibiting a NHase/Amidase activity ratio of at least 400 when being brought into contact with a nitrile compound to convert said nitrile compound into an amide compound.Type: GrantFiled: September 30, 2015Date of Patent: May 19, 2020Assignee: SOLENIS TECHNOLOGIES, L.P.Inventors: Michael Budde, Michael Braun, Juergen Daeuwel, Peter Oedman, Kai-Uwe Baldenius, Matthias Kleiner, Stephan Freyer
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Patent number: 10273514Abstract: The presently claimed invention is directed to a method for the preparation of an aqueous solution comprising at least one beta-glucan comprising at least the steps of a) fermentation of at least one fungal strain in an a fermentation broth, b) addition of at least one acid to the fermentation broth to adjust the pH to a value in the range of ?2.0 to ?4.0 and c) filtration of the fermentation broth to obtain an aqueous solution comprising the at least one beta-glucan and at least one beta-glucan that is obtained by this method.Type: GrantFiled: December 2, 2015Date of Patent: April 30, 2019Assignee: Wintershall Holding GmbHInventors: Sascha Rollie, Tobias Käppler, Carsten Schwalb, Florian Lehr, Andrea Hlubek, Jörg Therre, Christian Fleck, Thomas Letzelter, Stephan Freyer, Andrea Herold, Hansjörg Rettenmaier, Bernd Leonhardt
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Patent number: 10227621Abstract: The present invention relates to methods for preparing an aqueous acrylamide solution having a low acrylic acid concentration. In addition, the present invention relates to methods for reducing the acrylic acid concentration of an aqueous acrylamide solution. The methods involve a bioconversion of acrylonitrile to acrylamide in the presence of a biocatalyst, wherein during the bioconversion the content of acrylonitrile is maintained at 0.3 w/w % or more referred to the total weight of the composition in the reactor. Also provided is an aqueous acrylamide solution which is obtained by the methods of the present invention. Furthermore, the present invention is related to an acrylamide homopolymer or copolymer obtained by polymerizing the acrylamide of the aqueous solution.Type: GrantFiled: September 30, 2015Date of Patent: March 12, 2019Assignee: BASF SEInventors: Michael Guenter Braun, Juergen Daeuwel, Hans-Juergen Lang, Peter Oedman, Kai-Uwe Baldenius, Matthias Kleiner, Michael Kiefer, Stephan Freyer, Michael Budde
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Patent number: 10150777Abstract: The invention pertains to processes to produce dry biomass of pyripyropene producer organisms, processes to obtain pyripyropenes from such dry biomass, as well as to processes to produce compounds of Formula III and/or Formula IV and/or Formula V from the pyripyropenes obtained from the dry biomass. The invention does further pertain to the dry biomass itself, as well as processes using said dry biomass to obtain pyripyropenes for the production of compounds of Formula III and/or Formula IV and/or Formula V, including processes using said dry biomass to obtain pyripyropenes or compounds of Formula III and/or Formula IV and/or Formula V in order to produce pest control compositions, in particular insecticides, comprising such compounds.Type: GrantFiled: March 3, 2014Date of Patent: December 11, 2018Assignee: BASF SEInventors: Burkhard Ernst, Manfred Ehresmann, Christopher Koradin, Andreas Pletsch, Melanie Bonnekessel, Thomas Kaeding, Karin Schein-Albrecht, Stefanie Demming, Franz Weber, Wolfgang Siegel, Hartwig Schroeder, Stephan Freyer, Peter Oedman
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Publication number: 20180273647Abstract: The presently claimed invention relates to a method for concentrating a beta-glucan comprising at least the following steps: (a1) contacting an aqueous beta-glucan solution having a concentration [c1] with at least one precipitating agent p1 to obtain a precipitated beta-glucan in a solvent mixture comprising water and the at least one precipitating agent p1; (b1) separating the precipitated beta-glucan from the solvent mixture comprising water and the at least one precipitating agent p1 to obtain a precipitated beta-glucan having a concentration [c2]; and (c1) applying force to the precipitated beta-glucan of (b1) to obtain a precipitated beta-glucan having a concentration [c3].Type: ApplicationFiled: October 30, 2015Publication date: September 27, 2018Inventors: Sascha ROLLIE, Christoph ZIEGLER, Florian LEHR, Tobias KÄPPLER, Andrea SEIBERT, Thomas LETZELTER, Andreas WEBER, Stephan FREYER, Georg GROSSMANN, Burkhard Ernst
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Patent number: 10047035Abstract: The invention relates to a process for continuously preparing di-C1-3-alkyl succinates by reacting succinic acid with an C1-3-alkanol in the presence of a fixed-bed heterogeneous acidic esterification catalyst in a tubular reactor at a temperature in the range of from 60 to 100° C., wherein a mixture, comprising succinic acid, C1-3-alkanol, mono-C1-3-alkyl succinate, di-C1-3-alkyl succinate and water, is formed in a mixing stage and fed to the entrance of the tubular reactor, and wherein 5 to 75% of the outlet flow rate of the tubular reactor are recycled directly to the mixing stage as a recycle stream, and the molar ratio of C1-3-alkanol to succinic acid added to the mixing zone, and not including the C1-3-alkanol and succinic acid at the recycle stream, being in the range of from 2.0 to 9.5.Type: GrantFiled: July 25, 2017Date of Patent: August 14, 2018Assignee: BASF SEInventors: Stefanie Demming, Suman Thotla, Jens Wittenberg, Gabriele Iffland, Till Christian Brueggemann, Yvonne Hoelzl, Wolfgang Siegel, Stephan Freyer
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Publication number: 20170362620Abstract: The present invention relates to a process for the fermentation of fungal strains which secrete glucans with a ?-1,3-glycosidically linked main chain and side chains ?-1,6-glycosidically bonded thereto, in a cascade of tanks using high-shear mixers.Type: ApplicationFiled: December 8, 2015Publication date: December 21, 2017Inventors: Sebastian BRIECHLE, Rajan HOLLMANN, Tobias KÄPPLER, Florian LEHR, Julia Kristiane SCHMIDT, Stephan FREYER
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Publication number: 20170362619Abstract: The presently claimed invention is directed to a method for the preparation of an aqueous solution comprising at least one beta-glucan comprising at least the steps of a) fermentation of at least one fungal strain in an a fermentation broth, b) addition of at least one acid to the fermentation broth to adjust the pH to a value in the range of ?2.0 to ?4.0 and c) filtration of the fermentation broth to obtain an aqueous solution comprising the at least one beta-glucan and at least one beta-glucan that is obtained by this method.Type: ApplicationFiled: December 2, 2015Publication date: December 21, 2017Inventors: Sascha ROLLIE, Tobias KÄPPLER, Carsten SCHWALB, Florian LEHR, Andrea HLUBEK, Jörg THERRE, Christian FLECK, Thomas LETZELTER, Stephan FREYER, Andrea HEROLD, Hansjörg RETTENMAIER, Bernd LEONHARDT
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Publication number: 20170342015Abstract: The invention relates to a process for continuously preparing di-C1-3-alkyl succinates by reacting succinic acid with an C1-3-alkanol in the presence of a fixed-bed heterogeneous acidic esterification catalyst in a tubular reactor at a temperature in the range of from 60 to 100° C., wherein a mixture, comprising succinic acid, C1-3-alkanol, mono-C1-3-alkyl succinate, di-C1-3-alkyl succinate and water, is formed in a mixing stage and fed to the entrance of the tubular reactor, and wherein 5 to 75% of the outlet flow rate of the tubular reactor are recycled directly to the mixing stage as a recycle stream, and the molar ratio of C1-3-alkanol to succinic acid added to the mixing zone, and not including the C1-3-alkanol and succinic acid at the recycle stream, being in the range of from 2.0 to 9.5.Type: ApplicationFiled: July 25, 2017Publication date: November 30, 2017Applicant: BASF SEInventors: Stefanie Demming, Suman Thotla, Jens Wittenberg, Gabriele Iffland, Till Christian Brueggemann, Yvonne Hoelzl, Wolfgang Siegel, Stephan Freyer
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Publication number: 20170283840Abstract: The present invention relates to methods for preparing an aqueous acrylamide solution having a low acrylic acid concentration. In addition, the present invention relates to methods for reducing the acrylic acid concentration of an aqueous acrylamide solution. The methods involve a bioconversion of acrylonitrile to acrylamide in the presence of a biocatalyst, wherein during the bioconversion the content of acrylonitrile is maintained at 0.3 w/w % or more referred to the total weight of the composition in the reactor. Also provided is an aqueous acrylamide solution which is obtained by the methods of the present invention. Furthermore, the present invention is related to an acrylamide homopolymer or copolymer obtained by polymerizing the acrylamide of the aqueous solution.Type: ApplicationFiled: September 30, 2015Publication date: October 5, 2017Applicant: BASF SEInventors: Michael BRAUN, Juergen DAEUWEL, Hans-Juergen LANG, Peter OEDMAN, Kai-Uwe BALDENIUS, Matthias KLEINER, Michael KIEFER, Stephan FREYER, Michael BUDDE
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Publication number: 20170260519Abstract: The present invention relates to methods for cultivating a nitrile hydratase producing microorganism, compositions for cultivating a nitrile hydratase producing microorganism, and use of compositions comprising a saccharide and an organic acid for cultivating a nitrile hydratase producing microorganism. The composition provided in and to be employed in context with the present invention is particularly suitable for inducing both, growth and nitrile hydratase production of corresponding microorganisms.Type: ApplicationFiled: September 30, 2015Publication date: September 14, 2017Applicant: BASF SEInventors: Kerstin HAGE, Carsten SCHWALB, Petra SPROETE, Peter OEDMAN, Kai-Uwe BALDENIUS, Burkhard ERNST, Gunter STEIGELMANN, Stephan FREYER, Claus BOLLSCHWEILER, Michael Guenter BRAUN, Juergen DAEUWEL
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Publication number: 20170247726Abstract: The present invention relates to means and methods for producing an amide compound from a nitrile compound with less acrylic acid as by-product using a Nitrile hydratase (NHase) and Amidase producing microorganism as biocatalyst. Also provided is an aqueous amide compound obtained by the methods of the invention as well as a composition comprising acrylamide or polyacrylamide as well as a dried microorganism exhibiting a NHase/Amidase activity ratio of at least 400 when being brought into contact with a nitrile compound to convert said nitrile compound into an amide compound.Type: ApplicationFiled: September 30, 2015Publication date: August 31, 2017Applicant: BASF SEInventors: Michael BUDDE, Michael BRAUN, Juergen DAEUWEL, Peter OEDMAN, Kai-Uwe BALDENIUS, Matthias KLEINER, Stephan FREYER
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Patent number: 9738588Abstract: The invention relates to a process for continuously preparing di-C1-3-alkyl succinates by reacting succinic acid with an C1-3-alkanol in the presence of a fixed-bed heterogeneous acidic esterification catalyst in a tubular reactor at a temperature in the range of from 60 to 100° C., wherein a mixture, comprising succinic acid, C1-3-alkanol, mono-C1-3-alkyl succinate, di-C1-3-alkyl succinate and water, is formed in a mixing stage and fed to the entrance of the tubular reactor, and wherein 5 to 75% of the outlet flow rate of the tubular reactor are recycled directly to the mixing stage as a recycle stream, and the molar ratio of C1-3-alkanol to succinic acid added to the mixing zone, and not including the C1-3-alkanol and succinic acid at the recycle stream, being in the range of from 2.0 to 9.5.Type: GrantFiled: May 7, 2014Date of Patent: August 22, 2017Assignee: BASF SEInventors: Stefanie Demming, Suman Thotla, Jens Wittenberg, Gabriele Iffland, Christian Brueggemann, Yvonne Hoelzl, Wolfgang Siegel, Stephan Freyer
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Publication number: 20160083328Abstract: The invention relates to a process for continuously preparing di-C1-3-alkyl succinates by reacting succinic acid with an C1-3-alkanol in the presence of a fixed-bed heterogeneous acidic esterification catalyst in a tubular reactor at a temperature in the range of from 60 to 100° C., wherein a mixture, comprising succinic acid, C1-3-alkanol, mono-C1-3-alkyl succinate, di-C1-3-alkyl succinate and water, is formed in a mixing stage and fed to the entrance of the tubular reactor, and wherein 5 to 75% of the outlet flow rate of the tubular reactor are recycled directly to the mixing stage as a recycle stream, and the molar ratio of C1-3-alkanol to succinic acid added to the mixing zone, and not including the C1-3-alkanol and succinic acid at the recycle stream, being in the range of from 2.0 to 9.5.Type: ApplicationFiled: May 7, 2014Publication date: March 24, 2016Inventors: Stefanie Demming, Suman Thotla, Jens Wittenberg, Gabriele Iffland, Till Christian Brueggemann, Yvonne Hoelzl, Wolfgang Siegel, Stephan Freyer
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Publication number: 20160046645Abstract: The invention pertains to processes to produce dry biomass of pyripyropene producer organisms, processes to obtain pyripyropenes from such dry biomass, as well as to processes to produce compounds of Formula III and/or Formula IV and/or Formula V from the pyripyropenes obtained from the dry biomass. The invention does further pertain to the dry biomass itself, as well as processes using said dry biomass to obtain pyripyropenes for the production of compounds of Formula III and/or Formula IV and/or Formula V, including processes using said dry biomass to obtain pyripyropenes or compounds of Formula III and/or Formula IV and/or Formula V in order to produce pest control compositions, in particular insecticides, comprising such compounds.Type: ApplicationFiled: March 3, 2014Publication date: February 18, 2016Inventors: Burkhard ERNST, Manfred EHRESMANN, Christopher KORADIN, Andreas PLETSCH, Melanie BONNEKESSEL, Thomas KAEDING, Karin SCHEIN-ALBRECHT, Stefanie DEMMING, Franz WEBER, Wolfgang SIEGEL, Hartwig SCHROEDER, Stephan FREYER, Peter OEDMAN
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Publication number: 20160002363Abstract: The present invention relates to an improved process for filtering aqueous fermentation broths comprising glucans and biomass using symmetrical tubular membranes.Type: ApplicationFiled: February 26, 2014Publication date: January 7, 2016Inventors: Jörg Therre, Hartwig Voß, Tobias Käppler, Sascha Rollie, Stephan Freyer, Bernd Leonhardt