Abstract: Devices and methods for selectively accessing tissue for sensing or drug release are provided. A device includes an array of wells formed in a substrate supporting a plurality of membranes. Each membrane is disposed at a well opening of one of the wells of the array. The device further includes an actuator and electronics configured to control the actuator to supply a vibration through the substrate. The supplied vibration is configured to selectively rupture one of the plurality of membranes at a defined timepoint to selectively give access to tissue through a well opening.

Abstract: Embodiments of the disclosure provide a method for controlling a power generation system during on-line maintenance. The method includes operating the power generation system in a first maintenance mode, which causes a controller of the power generation system to disable an automated response to at least one operational fault of the power generation system; monitoring a risk parameter of the power generation system or at least one sensor within the power generation system while operating the power generation system in the first maintenance mode; and operating the power generation system in a second maintenance mode in response to detecting an override command, an elapsed time exceeding a time limit, or the monitored risk parameter exceeding a safety threshold. The second maintenance mode causes the controller of the power generation system to enable the automated response to the at least one operational fault of the power generation system.

Abstract: Non-naturally occurring thermogenic adipocytes are provided. The cells have a distinctive molecular signature, and can be distinguished from predecessor cells such as adipose-derived stem cells as well as other naturally occurring and induced thermogenic cells. Differentiation media that can induce differentiation of ADSC and white adipocytes into thermogenic adipocytes is also provided. Methods of making thermogenic adipocytes, thermogenic adipocytes made according the disclosed methods as well as conditioned media made according a method of incubating the cells in a tissue culture media are also provided. Compositions including thermogenic adipocytes, conditioned media, secreted factor(s), active agents that increase the number or activity of thermogenic adipocytes, or a combination thereof are also disclosed and can be used to treat a variety of diseases and conditions, particularly obesity and metabolic disorders.

Abstract: Embodiments of the disclosure provide a method for controlling a power generation system during on-line maintenance. The method includes operating the power generation system in a first maintenance mode, which causes a controller of the power generation system to disable an automated response to at least one operational fault of the power generation system; monitoring a risk parameter of the power generation system or at least one sensor within the power generation system while operating the power generation system in the first maintenance mode; and operating the power generation system in a second maintenance mode in response to detecting an override command, an elapsed time exceeding a time limit, or the monitored risk parameter exceeding a safety threshold. The second maintenance mode causes the controller of the power generation system to enable the automated response to the at least one operational fault of the power generation system.

Abstract: Use of a transforming growth factor beta (TGF-?) signalling inhibitor for producing a population of brown adipocytes in vitro.

Abstract: A method of installing a fence is provided including advancing a plurality of beams into the ground so that the beams achieve a substantially vertical orientation, and installing a plurality of tubular posts over the beams so that the posts automatically attain the substantially vertical orientation, without having to dispose any other levelling structures between the beams and the posts. One or more horizontal rails can be installed relative to adjacent beams and tubular posts. The tubular posts can define holes, and the opposing ends of a rail can be inserted through corresponding holes. Where the beams have longitudinal channels, the ends of the rails can project through the holes in the posts and into those channels. The tubular posts and/or rails can be secured in place with fasteners. The method provides an inexpensive and quick way to install a fence without digging holes for the beams.

Abstract: The present invention is directed to vascular tissue constructs or vessels and methods for producing vascular tissue constructs or vessels (ie., fabricated blood vessels), including veins, arteries, capillaries and other vascular structures from a biomaterial foundation or scaffold and epicardial progenitor cells (EPCs) which are seeded onto the biomaterial, exposed to a differentiation medium and differentiated into endothelial, cells, smooth muscle cells and pericytes which self-assemble into vascular tissue associated with N the biomaterial foundation or scaffold.

Abstract: A method of installing a fence is provided including advancing a plurality of beams into the ground so that the beams achieve a substantially vertical orientation, and installing a plurality of tubular posts over the beams so that the posts automatically attain the substantially vertical orientation, without having to dispose any other levelling structures between the beams and the posts. One or more horizontal rails can be installed relative to adjacent beams and tubular posts. The tubular posts can define holes, and the opposing ends of a rail can be inserted through corresponding holes. Where the beams have longitudinal channels, the ends of the rails can project through the holes in the posts and into those channels. The tubular posts and/or rails can be secured in place with fasteners. The method provides an inexpensive and quick way to install a fence without digging holes for the beams.

Abstract: The present invention relates to the differentiation of human pluripotent cells, including human pluripotent stems cells to produce a self-renewing multipotent neural crest cell population in a single step method without the requirement of isolation of intermediate cells and without appreciable contamination (in certain preferred instances, virtually none) with Pax6+ neural progenitor cells in the population of p75+Hnk1+Ap2+ multipotent neural crest-like cells. The multipotent neural crest cell population obtained can be clonally amplified and maintained for >25 passages (>100 days) while retaining the capacity to differentiate into peripheral neurons, smooth muscle cells and mesenchymal precursor cells.

Abstract: The present invention relates to the differentiation of human pluripotent cells, including human pluripotent stems cells to produce a self-renewing multipotent neural crest cell population in a single step method without the requirement of isolation of intermediate cells and without appreciable contamination (in certain preferred instances, virtually none) with Pax6+ neural progenitor cells in the population of p75+ Hnk1+ Ap2+ multipotent neural crest-like cells. The multipotent neural crest cell population obtained can be clonally amplified and maintained for >25 passages (>100 days) while retaining the capacity to differentiate into peripheral neurons, smooth muscle cells and mesenchymal precursor cells.

Abstract: An interface appliance is configured to deliver a flow of fluid to the airway of a subject. The generation of the flow of fluid may be controlled to provide a therapeutic benefit to the subject. The interface appliance is configured to carry one or more sensors (24) configured to detect one or more parameters related to the flow of fluid on the subject. The one or more parameters may then be used to control the generation and/or delivery of the flow of fluid.

Abstract: A system and method are configured to adjust the tidal volume of the breathing of a subject. The subject is self-ventilating (breathes under her own power). The adjustment of tidal volume accomplished through use of the system and/or method may reduce hypertension (e.g., lower blood pressure), reduce stress and/or anxiety (and related maladies), improve relaxation, decrease sleep latency, improve sleep quality, address other sleep disorders, and/or provide other health benefits. The system and method are effective in adjusting tidal volume while the subject is awake and/or asleep.

Abstract: A pressurized flow of breathable gas is delivered to the airway of a subject through a gas circuit (16) as part of a therapy regime. Leakage of gas from within the circuit to atmosphere to prevent re-breathing of gas is dynamically adjusted to stabalize the total amount of leakage.

Abstract: The present invention relates to inter alia, methods for the generation and maintenance of Mesoderm-derived ISL1+ Multipotent Progenitors (IMPs), the production of a number of pluripotent cells including and epicardial pluripotent cells (EPCs) and using these cells to produce endothelial cells, cardiomyocytes, smooth muscle cells, vascular cells and other cells and related methods as otherwise disclosed herein. The invention also relates to compositions comprising a population of cells.

Abstract: The present invention relates to the differentiation of human pluripotent cells, including human pluripotent stems cells to produce a self-renewing multipotent neural crest cell population in a single step method without the requirement of isolation of intermediate cells and without appreciable contamination (in certain preferred instances, virtually none) with Pax6+ neural progenitor cells in the population of p75+Hnk1+Ap2+ multipotent neural crest-like cells. The multipotent neural crest cell population obtained can be clonally amplified and maintained for >25 passages (>100 days) while retaining the capacity to differentiate into peripheral neurons, smooth muscle cells and mesenchymal precursor cells.

Abstract: The present invention relates to inter alia, methods for the generation and maintenance of Mesoderm-derived ISL1+ Multipotent Progenitors (IMPs), the production of a number of pluripotent cells including and epicardial pluripotent cells (EPCs) and using these cells to produce endothelial cells, cardiomyocytes, smooth muscle cells, vascular cells and other cells and related methods as otherwise disclosed herein. The invention also relates to compositions comprising a population of cells.

Abstract: The present invention relates to a method of isotopically labeling glycans and in facilitating high throughput quantitative/comparative analysis of glycomic compositions of biological cells. The method is applicable inter alia for identifying differentiated cells and their glycomic characteristics, differentiation conditions, disease and/or therapeutic progression, diagnosing disease states, determining drug activity, establishing manufacturing efficiencies and for determining the half-life of glycans in cells.

Abstract: The present invention relates to the production of multipotent migratory cell (MMCs) which can be differentiated into mesoderm or endoderm lineages. Multipotent Migratory Cells (MMC) are stable and robust and can be passaged at least 20 times (perhaps indefinitely), can be recovered after freezing, reamplified and differentiated into multiple lineages. They are therefore storage stable. The method of producing these cells points to a way to generate a multipotent cell type (mesendoderm) from blastocycts for the generation of therapeutically useful cell types without going through a classical hESC state. The production of multipotent migratory cells, mesendoderm cells and mesoderm cells (Isl1+) is also described.

Abstract: Embodiments of the present invention have the technical effect of automatically testing an overspeed protection system of a powerplant machine comprising at least one shaft. An embodiment of the present invention may automatically test the overspeed protection system while the powerplant machine is decelerating from full-speed-no-load (FSNL). Another embodiment of the present invention may automatically test the overspeed protection system of the powerplant machine while the powerplant machine is accelerating to FSNL.

Abstract: The present invention relates to the differentiation of human pluripotent cells, including human pluripotent stems cells to produce a self-renewing multipotent neural crest cell population in a single step method without the requirement of isolation of intermediate cells and without appreciable contamination (in certain preferred instances, virtually none) with Pax6+ neural progenitor cells in the population of p75+ Hnk1+ Ap2+ multipotent neural crest-like cells. The multipotent neural crest cell population obtained can be clonally amplified and maintained for >25 passages (>100 days) while retaining the capacity to differentiate into peripheral neurons, smooth muscle cells and mesenchymal precursor cells.