Abstract: Embodiments of the present invention have the technical effect of automatically testing an overspeed protection system of a powerplant machine comprising a shaft and integrated with a safety control system. An embodiment of the present invention may automatically test the overspeed protection system while the powerplant machine is in the process of shutting down. Another embodiment of the present invention may automatically test the overspeed protection system of the powerplant machine by adjusting the speed of a shaft while operating at full-speed-no-load.

Abstract: Embodiments of the present invention have the technical effect of determining when to test an overspeed protection system of a powerplant machine. As described herein, embodiments of the present invention may be applied to a wide variety of powerplant machines, each comprising a shaft. After determining that test of the overspeed protection system should be performed, embodiments of the present invention may allow for a variety of methods to test the overspeed protection system.

Abstract: An interface appliance is configured to deliver a flow of fluid to the airway of a subject. The generation of the flow of fluid may be controlled to provide a therapeutic benefit to the subject. The interface appliance is configured to carry one or more sensors (24) configured to detect one or more parameters related to the flow of fluid on the subject. The one or more parameters may then be used to control the generation and/or delivery of the flow of fluid.

Abstract: The present invention provides compositions and methods for the production of differentiated mammalian cells. More particularly, the present invention provides cellular differentiation methods employing culturing the cells on a feeder layer or under feeder-free conditions in cell culture and further contacting the cells with an inhibitor of the PI3-kinase pathway and a member of the TGFb family for the generation of differentiated mammalian cells from pluripotent mammalian stem cells. Preferably, the differentiated cell is selected from the group consisting of a mesendodermal cell, a mesodermal cell, and an endodermal cell.

Abstract: A pressurized flow of breathable gas is delivered to the airway of a subject through a gas circuit (16) as part of a therapy regime. Leakage of gas from within the circuit to atmosphere to prevent re-breathing of gas is dynamically adjusted to stabalize the total amount of leakage.

Abstract: A system and method are implemented to identify pulmonary congestion in a subject. The system and method detect pulmonary congestion in a subject based on one or more parameters of the breathing of the subject. The detection of pulmonary congestion in the subject may be relatively passive for the subject. This may enhance the convenience and/or comfort of compliance to a detection regime by the subject. By detecting pulmonary congestion in the subject, potential episodes of heart failure may be identified and/or averted. The system and method may be configured to facilitate treatment of the subject to remediate detected pulmonary congestion and/or avert potential episodes of heart failure. For example, the system may provide enhanced access for a caregiver to detections of parameters related to pulmonary congestion in the subject.

Abstract: A system and method are configured to adjust the tidal volume of the breathing of a subject. The subject is self-ventilating (breathes under her own power). The adjustment of tidal volume accomplished through use of the system and/or method may reduce hypertension (e.g., lower blood pressure), reduce stress and/or anxiety (and related maladies), improve relaxation, decrease sleep latency, improve sleep quality, address other sleep disorders, and/or provide other health benefits. The system and method are effective in adjusting tidal volume while the subject is awake and/or asleep.

Abstract: The present invention relates to inter alia, methods for the generation and maintenance of Mesoderm-derived ISL1+ Multipotent Progenitors (IMPs), the production of a number of pluripotent cells including and epicardial pluripotent cells (EPCs) and using these cells to produce endothelial cells, cardiomyocytes, smooth muscle cells, vascular cells and other cells and related methods as otherwise disclosed herein. The invention also relates to compositions comprising a population of cells.

Abstract: Embodiments of the present invention have the technical effect of automatically testing an overspeed protection system of a powerplant machine comprising at least one shaft. An embodiment of the present invention may automatically test the overspeed protection system while the powerplant machine is decelerating from full-speed-no-load (FSNL). Another embodiment of the present invention may automatically test the overspeed protection system of the powerplant machine while the powerplant machine is accelerating to FSNL.

Abstract: Embodiments of the present invention have the technical effect of automatically testing an overspeed protection system of a powerplant machine comprising a shaft and integrated with a safety control system. An embodiment of the present invention may automatically test the overspeed protection system while the powerplant machine is in the process of shutting down. Another embodiment of the present invention may automatically test the overspeed protection system of the powerplant machine by adjusting the speed of a shaft while operating at full-speed-no-load.

Abstract: Embodiments of the present invention have the technical effect of determining when to test an overspeed protection system of a powerplant machine. As described herein, embodiments of the present invention may be applied to a wide variety of powerplant machines, each comprising a shaft. After determining that test of the overspeed protection system should be performed, embodiments of the present invention may allow for a variety of methods to test the overspeed protection system.

Abstract: The present invention relates to a method of isotopically labeling glycans and in facilitating high throughput quantitative/comparative analysis of glycomic compositions of biological cells. The method is applicable inter alia for identifying differentiated cells and their glycomic characteristics, differentiation conditions, disease and/or therapeutic progression, diagnosing disease states, determining drug activity, establishing manufacturing efficiencies and for determining the half-life of glycans in cells.

Abstract: The present invention relates to the production of multipotent migratory cell (MMCs) which can be differentiated into mesoderm or endoderm lineages. Multipotent Migratory Cells (MMC) are stable and robust and can be passaged at least 20 times (perhaps indefinitely), can be recovered after freezing, reamplified and differentiated into multiple lineages. They are therefore storage stable. The method of producing these cells points to a way to generate a multipotent cell type (mesendoderm) from blastocycts for the generation of therapeutically useful cell types without going through a classical hESC state. The production of multipotent migratory cells, mesendoderm cells and mesoderm cells (Isl1+) is also described.

Abstract: The invention relates to methods that allow for the efficient differentiation to form pancreatic endoderm cells from pluripotent stem cells such as human embryonic stem cells and definitive endoderm cells. The invention is directly applicable to the ultimate generation of pancreatic beta cells that could be used as part of a therapy to treat or even cure diabetes. Additionally, the present invention may be used to generate liver endoderm cells from human embryonic stem cells and definite endoderm cells as well. This invention relates to a method for generating definitive endoderm and pancreatic endoderm cells from stem cells, preferably human embryonic stem cells using defined media in the absence of feeder cells. A simply two step procedure to provide pancreatic endoderm cells from embryonic stem cells represents further embodiments of the present invention.

Abstract: The present invention provides methods for stabilizing pluripotent cells through the transcriptional activation of c-myc. Alternatively, the cells are stabilized through the transcriptional activation of c-myc, and the stabilization of c-myc protein levels. c-myc protein can be stabilized through the inhibition of GSK3? or through other components of the cellular machinery that impact on c-myc stability. The invention contemplates the stabilized pluripotent cells produced using the methods described herein. Methods for the identification of compounds that modulate the stabilization of pluripotent cells through modulating transcriptional activation of c-myc, stabilization of c-myc protein levels, and/or inhibition of GSK3? activity are also contemplated.

Abstract: The present invention provides compositions and methods for the production of differentiated mammalian cells. More particularly, the present invention provides cellular differentiation methods employing culturing the cells on a feeder layer or under feeder-free conditions in cell culture and further contacting the cells with an inhibitor of the PI3-kinase pathway for the generation of differentiated mammalian cells from pluripotent mammalian stem cells. Preferably, the differentiated cell is selected from the group consisting of a mesendodermal cell, a mesodermal cell, and an endodermal cell.

Abstract: The G.A.M. machine or design can be adapted to any size of power source. The engineering calculus has been instructed at today's date which will result in an exact science of the use of weight, gravity and measurements of distance involved to create the torque or resistance. The machine requires no fossil fuels nor does it omit any poisonous gases or effluent. The workings housed within the arms air compressor, piston, piston shaft and weight will be combined in a unique system to ultimately result in the rotation of the arms (windmill style) which will turn a drive shaft to generate power for any purpose.

Abstract: A method of identifying and/or selecting non-differentiating pluripotent or pluripotent-related cells from a mixed cell population including differentiating and non-differentiating pluripotent or pluripotent-related cells, which method includes reducing kinase activity and/or expression in said cells.

Abstract: A method of regulating the differentiation potential of a pluripotent or pluripotent-related cell which method Includes manipulating the expression and/or activity of a cell differentiation regulatory molecule in a pluripotent or pluripotent related cell.

Abstract: Methods are provided for studying protein-protein interactions which require posttranslational modification of one of the proteins. The interaction is detected by reconstituting the activity of a transcriptional activator. This activity is dependent on the interactions between three different proteins. These include two chimeric proteins, one of which must be posttranslationally modified by the activity of the third protein in order for the chimeric proteins to interact. One of the chimeric proteins contains a transcriptional activation domain fused to a test protein. The second chimeric protein contains a DNA-binding domain of a transcriptional activator fused to the other test protein.