Patents by Inventor Stephen Quake

Stephen Quake has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20220017958
    Abstract: The invention provides compositions and methods for simultaneously determining the presence or absence of fetal aneuploidy and the relative amount of fetal nucleic acids in a sample obtained from a pregnant female. The method encompasses the use of sequencing technologies and exploits the occurrence of polymorphisms to provide a streamlined noninvasive process applicable to the practice of prenatal diagnostics.
    Type: Application
    Filed: September 2, 2021
    Publication date: January 20, 2022
    Applicant: Verinata Health, Inc.
    Inventors: Stephen Quake, Richard P. Rava, Manjula Chinnappa, David A. Comstock, Gabrielle Heilek
  • Patent number: 11130995
    Abstract: The invention provides compositions and methods for simultaneously determining the presence or absence of fetal aneuploidy and the relative amount of fetal nucleic acids in a sample obtained from a pregnant female. The method encompasses the use of sequencing technologies and exploits the occurrence of polymorphisms to provide a streamlined noninvasive process applicable to the practice of prenatal diagnostics.
    Type: Grant
    Filed: July 31, 2017
    Date of Patent: September 28, 2021
    Assignee: Verinata Health, Inc.
    Inventors: Stephen Quake, Richard P Rava, Manjula Chinnappa, David A Comstock, Gabrielle Heilek
  • Publication number: 20210079470
    Abstract: Methods for detection of single nucleotide mutations of autosomal recessive diseases as early as the first trimester of pregnancy are provided. This is of importance for metabolic disorders where early diagnosis can affect management of the disease and reduce complications and anxiety related to invasive testing.
    Type: Application
    Filed: July 6, 2018
    Publication date: March 18, 2021
    Inventors: Stephen Quake, Joan Camunas-Soler
  • Publication number: 20180346984
    Abstract: The present methods are exemplified by a process in which maternal blood containing fetal DNA is diluted to a nominal value of approximately 0.5 genome equivalent of DNA per reaction sample. Digital analysis is then be used to detect aneuploidy, such as the trisomy that causes Down Syndrome. Since aneuploidies do not present a mutational change in sequence, and are merely a change in the number of chromosomes, it has not been possible to detect them in a fetus without resorting to invasive techniques such as amniocentesis or chorionic villi sampling. Digital amplification allows the detection of aneuploidy using massively parallel amplification and detection methods, examining, e.g., 10,000 genome equivalents.
    Type: Application
    Filed: August 3, 2018
    Publication date: December 6, 2018
    Applicant: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Stephen Quake, Hei-Mun Christina Fan
  • Patent number: 10072295
    Abstract: The present methods are exemplified by a process in which maternal blood containing fetal DNA is diluted to a nominal value of approximately 0.5 genome equivalent of DNA per reaction sample. Digital analysis is then be used to detect aneuploidy, such as the trisomy that causes Down Syndrome. Since aneuploidies do not present a mutational change in sequence, and are merely a change in the number of chromosomes, it has not been possible to detect them in a fetus without resorting to invasive techniques such as amniocentesis or chorionic villi sampling. Digital amplification allows the detection of aneuploidy using massively parallel amplification and detection methods, examining, e.g., 10,000 genome equivalents.
    Type: Grant
    Filed: December 23, 2014
    Date of Patent: September 11, 2018
    Assignee: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
    Inventors: Stephen Quake, Hei-Mun Christina Fan
  • Publication number: 20170327883
    Abstract: The invention provides compositions and methods for simultaneously determining the presence or absence of fetal aneuploidy and the relative amount of fetal nucleic acids in a sample obtained from a pregnant female. The method encompasses the use of sequencing technologies and exploits the occurrence of polymorphisms to provide a streamlined noninvasive process applicable to the practice of prenatal diagnostics.
    Type: Application
    Filed: July 31, 2017
    Publication date: November 16, 2017
    Inventors: Stephen Quake, Richard P Rava, Manjula Chinnappa, David A Comstock, Gabrielle Heilek
  • Patent number: 9777329
    Abstract: The present methods are exemplified by a process in which maternal blood containing fetal DNA is diluted to a nominal value of approximately 0.5 genome equivalent of DNA per reaction sample. Digital PCR is then be used to detect aneuploidy, such as the trisomy that causes Down Syndrome. Since aneuploidies do not present a mutational change in sequence, and are merely a change in the number of chromosomes, it has not been possible to detect them in a fetus without resorting to invasive techniques such as amniocentesis or chorionic villi sampling. Digital amplification allows the detection of aneuploidy using massively parallel amplification and detection methods, examining, e.g., 10,000 genome equivalents.
    Type: Grant
    Filed: January 19, 2010
    Date of Patent: October 3, 2017
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Stephen Quake, Hei-Mun Christina Fan
  • Patent number: 9777328
    Abstract: The present methods are exemplified by a process in which maternal blood containing fetal DNA is diluted to a nominal value of approximately 0.5 genome equivalent of DNA per reaction sample. Digital PCR is then be used to detect aneuploidy, such as the trisomy that causes Down Syndrome. Since aneuploidies do not present a mutational change in sequence, and are merely a change in the number of chromosomes, it has not been possible to detect them in a fetus without resorting to invasive techniques such as amniocentesis or chorionic villi sampling. Digital amplification allows the detection of aneuploidy using massively parallel amplification and detection methods, examining, e.g., 10,000 genome equivalents.
    Type: Grant
    Filed: January 19, 2010
    Date of Patent: October 3, 2017
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Stephen Quake, Hei-Mun Christina Fan
  • Patent number: 9441273
    Abstract: The present methods are exemplified by a process in which maternal blood containing fetal DNA is diluted to a nominal value of approximately 0.5 genome equivalent of DNA per reaction sample. Digital PCR is then be used to detect aneuploidy, such as the trisomy that causes Down Syndrome. Since aneuploidies do not present a mutational change in sequence, and are merely a change in the number of chromosomes, it has not been possible to detect them in a fetus without resorting to invasive techniques such as amniocentesis or chorionic villi sampling. Digital amplification allows the detection of aneuploidy using massively parallel amplification and detection methods, examining, e.g., 10,000 genome equivalents.
    Type: Grant
    Filed: June 15, 2010
    Date of Patent: September 13, 2016
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Stephen Quake, Hei-Mun Christina Fan
  • Publication number: 20150104793
    Abstract: The present methods are exemplified by a process in which maternal blood containing fetal DNA is diluted to a nominal value of approximately 0.5 genome equivalent of DNA per reaction sample. Digital analysis is then be used to detect aneuploidy, such as the trisomy that causes Down Syndrome. Since aneuploidies do not present a mutational change in sequence, and are merely a change in the number of chromosomes, it has not been possible to detect them in a fetus without resorting to invasive techniques such as amniocentesis or chorionic villi sampling. Digital amplification allows the detection of aneuploidy using massively parallel amplification and detection methods, examining, e.g., 10,000 genome equivalents.
    Type: Application
    Filed: December 23, 2014
    Publication date: April 16, 2015
    Applicant: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
    Inventors: STEPHEN QUAKE, HEI-MUN CHRISTINA FAN
  • Publication number: 20140326400
    Abstract: A fabrication method of a micromechanical valve includes: (1) forming a control layer according to a first weight ratio of cross linker: elastomer base; (2) forming a flow layer according to a second weight ratio of cross linker: elastomer base; (3) forming a membrane layer according to a third weight ratio of cross linker: elastomer base, where the third weight ratio is smaller than the first weight ratio, and is smaller than the second weight ratio; (4) bonding the membrane layer to the control layer to form a two-layer structure; and (5) bonding the two-layer structure to the flow layer to form the micromechanical valve.
    Type: Application
    Filed: April 30, 2014
    Publication date: November 6, 2014
    Inventors: Ismail Emre Araci, Stephen Quake, Michael Robles, Daniel Bechstein
  • Publication number: 20130001083
    Abstract: The invention relates to a microfabricated device and methods of using the device for analyzing and sorting polynucleotide molecules by size.
    Type: Application
    Filed: March 22, 2012
    Publication date: January 3, 2013
    Applicant: California Institute of Technology
    Inventors: Stephen Quake, Wayne D. Volksmuth
  • Patent number: 8293470
    Abstract: The present methods are exemplified by a process in which maternal blood containing fetal DNA is diluted to a nominal value of approximately 0.5 genome equivalent of DNA per reaction sample. Digital PCR is then be used to detect aneuploidy, such as the trisomy that causes Down Syndrome. Since aneuploidies do not present a mutational change in sequence, and are merely a change in the number of chromosomes, it has not been possible to detect them in a fetus without resorting to invasive techniques such as amniocentesis or chorionic villi sampling. Digital amplification allows the detection of aneuploidy using massively parallel amplification and detection methods, examining, e.g., 10,000 genome equivalents.
    Type: Grant
    Filed: June 15, 2010
    Date of Patent: October 23, 2012
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Stephen Quake, Hei-Mun Christina Fan
  • Publication number: 20120165203
    Abstract: The invention provides compositions and methods for simultaneously determining the presence or absence of fetal aneuploidy and the relative amount of fetal nucleic acids in a sample obtained form a pregnant female. The method encompasses the use of sequencing technologies and exploits the occurrence of polymorphisms to provide a streamlined noninvasive process applicable to the practice of prenatal diagnostics.
    Type: Application
    Filed: February 2, 2012
    Publication date: June 28, 2012
    Applicant: Verinata Health, Inc.
    Inventors: Stephen Quake, Richard P. Rava, Manjula Chinnappa, David A. Comstock, Gabrielle Heilek
  • Publication number: 20120156731
    Abstract: Disclosed are methods and materials for assembling long polynucleotides from synthetic oligonucleotides. The use of synthetic oligonucleotides permits non-natural design of sequences. The oligonucleotides used for construction may be relatively short, according to practicalities of nucleotide synthesis. They are assembled using a ligase which is operative over a range of temperatures, i.e., is thermostable. The method and oligonucleotides are designed such that the melting temperature of the strands to be hybridized is set at a number of selected specific temperatures for each group of oligonucleotides to be hybridized and ligated. Hybridization and ligation take place at or near the melting temperature, so that each succeeding ligation is governed by a temperature that will prevent hybridization if any mismatches are present.
    Type: Application
    Filed: March 20, 2008
    Publication date: June 21, 2012
    Applicant: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Yanyi Huang, James Berger, Stephen Quake
  • Publication number: 20120053335
    Abstract: Microfluidic nucleic acid hybridization systems are described that include a first reaction chamber to hold an analyte solution comprising nucleic acids, and a first mixing channel in fluid communication with the chamber. The mixing channel includes a textured surface to mix the analyte solution. The systems may also include pump coupled to the mixing channel to circulate the analyte solution through the reaction chamber and the mixing channel, and an input port in fluid communication with the mixing channel and the reaction chamber to supply the analyte solution to the microfluidic system. The input port can be closed to create a closed circulation path for the analyte solution through the reaction chamber and the mixing channel.
    Type: Application
    Filed: October 6, 2011
    Publication date: March 1, 2012
    Applicant: California Institute of Technology
    Inventors: Jian Liu, Brian A. Williams, Barbara J. Wold, Stephen Quake
  • Patent number: 8104497
    Abstract: A method of fabricating an elastomeric structure, comprising: forming a first elastomeric layer on top of a first micromachined mold, the first micromachined mold having a first raised protrusion which forms a first recess extending along a bottom surface of the first elastomeric layer; forming a second elastomeric layer on top of a second micromachined mold, the second micromachined mold having a second raised protrusion which forms a second recess extending along a bottom surface of the second elastomeric layer; bonding the bottom surface of the second elastomeric layer onto a top surface of the first elastomeric layer such that a control channel forms in the second recess between the first and second elastomeric layers; and positioning the first elastomeric layer on top of a planar substrate such that a flow channel forms in the first recess between the first elastomeric layer and the planar substrate.
    Type: Grant
    Filed: March 13, 2007
    Date of Patent: January 31, 2012
    Assignee: California Institute of Technology
    Inventors: Marc Unger, Hou-Pu Chou, Todd Thorsen, Axel Scherer, Stephen Quake, Markus Enzelberger, Mark Adams, Carl Hansen
  • Publication number: 20110275523
    Abstract: The present invention features methods for analyzing a sequence of a target polynucleotide by detecting incorporation of a nucleotide into its complementary strand, where the polynucleotides may be bound at high density and at single molecule resolution. The invention also features labeling moieties and blocking moieties, which facilitate chain termination or choking. Certain aspects provide for temporal detection of the incorporations; some allow for asynchronous analysis of a plurality of target polynucleotides and the use of short sequencing cycles. Surface chemistry aspects of the sequencing methods are also provided. The method may also be used in kits, said kits designed to carry out and facilitate the methods provided herein.
    Type: Application
    Filed: May 5, 2011
    Publication date: November 10, 2011
    Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventor: Stephen Quake
  • Publication number: 20110224087
    Abstract: The invention provides compositions and methods for simultaneously determining the presence or absence of fetal aneuploidy and the relative amount of fetal nucleic acids in a sample obtained form a pregnant female. The method encompasses the use of sequencing technologies and exploits the occurrence of polymorphisms to provide a streamlined noninvasive process applicable to the practice of prenatal diagnostics.
    Type: Application
    Filed: December 1, 2010
    Publication date: September 15, 2011
    Inventors: Stephen Quake, Richard P. Rava, Manjula Chinnappa, David A. Comstock, Gabrielle Heilek
  • Patent number: 8008018
    Abstract: The present methods are exemplified by a process in which maternal blood containing fetal DNA is diluted to a nominal value of approximately 0.5 genome equivalent of DNA per reaction sample. Digital PCR is then be used to detect aneuploidy, such as the trisomy that causes Down Syndrome. Since aneuploidies do not present a mutational change in sequence, and are merely a change in the number of chromosomes, it has not been possible to detect them in a fetus without resorting to invasive techniques such as amniocentesis or chorionic villi sampling. Digital amplification allows the detection of aneuploidy using massively parallel amplification and detection methods, examining, e.g., 10,000 genome equivalents.
    Type: Grant
    Filed: February 26, 2009
    Date of Patent: August 30, 2011
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Stephen Quake, Hei-Mun Christina Fan