Abstract: The present invention pertains to single plasmid systems comprising sequences encoding programmable proteins, one or more guides, optionally donor polynucleotides, and optionally anti-CRISPR molecules, for gene editing. These plasmid systems allow for genomic engineering of bacterial strains that are difficult to transform and increase the efficiency of genomic engineering in tractable strains. Additionally, the single plasmids can be configured to provide for the transformation of a number of different bacterial strains using the same plasmid.

Abstract: A playout system for providing playout of fragmented video content is provided. The system includes a non-transitory data store and a processor. The processor is configured to playout content for video origination using a fragmented format. The processor can receive a playlist and generate a plurality of video fragments from source content. A transcoder can process video fragments based on one or more program scheduling requirements from the playlist to generate new video fragments. A manifest is updated to reference the new video fragments. The process can output a video stream based on the new video fragments.

Abstract: A handheld cryoanesthesia or analgesia device for cooling a target area on cutaneous membranes, mucous membranes, and tissue of the mucocutaneous zone having an elongated body and a thermoelectric cooling system disposed within the elongated body. The thermoelectric cooling system is configured to physically contact and thermally couple the target area of the cutaneous membranes, mucous membranes, and tissue of the mucocutaneous zone to induce cryoanesthesia or analgesia. The thermoelectric cooling system includes a thermally-conductive cold tip, a thermally-conductive cooling power concentrator thermally coupled to the cold tip, at least one Peltier unit module thermally coupled to the cooling power concentrator, a heatsink thermally coupled to at least one Peltier unit module, a power source, at least one thermal sensor, and a controller operably outputting a control signal to the Peltier unit module to maintain a predetermined temperature.

Abstract: A system for producing fragmented video content is provided. The system includes a processing device, a non-transitory data store, and a memory storage device having at least a Manifest Processing Service (MPS) unit stored therein. The MPS unit is configured to generate at least one master manifest and store the master manifest in the non-transitory data store, the master manifest comprising a timing reference for a video fragment of the fragmented video content, wherein the timing reference indicates a temporal boundary of processing for the video fragment.

Abstract: An example method includes obtaining a plurality of data items. Each data item includes an indication of a particular location, an indication that a wireless signal from a first access point was observed at that location, and an indication of a time at which the wireless signal from the first access point was observed at that location. The method also includes determining a locational stability of the first access point based on the data items. Determining the locational stability of the first access point includes clustering the plurality of data items into one or more clusters based on the locations indicated in the plurality of data items, determining whether the N most recent data items are associated with a common cluster, and determining whether a time span between the N most recent data items exceeds a threshold period of time.

Abstract: Systems, methods, and non-transitory computer-readable media can receive a first contextual content item created by a first user. A user input is received from a second user indicating an intent to acquire the first contextual content item. The second user is provided with access to utilize the first contextual content item.

Abstract: A playout system for providing playout of fragmented video content is provided. The system includes a non-transitory data store and a processor. The processor is configured to playout content for video origination using a fragmented format. The processor can receive a playlist and generate a plurality of video fragments from source content. A transcoder can process video fragments based on one or more program scheduling requirements from the playlist to generate new video fragments. A manifest is updated to reference the new video fragments. The process can output a video stream based on the new video fragments.

Abstract: An electrostatic discharge (ESD) robust design for an input device such as a stylus is disclosed. The input device can include one or more components, such as one or more Schottky diodes, that can be damaged by ESD events. To reduce the likelihood of damage to sensitive components, the parasitic capacitance between sensitive conductive paths and reference ground paths of the input device that could otherwise provide electrostatic discharge paths can be reduced (arranging current limiting resistance at specific locations among sensitive components, creating physical separation between sensitive conductive paths and reference ground paths), shielding can be added to shield the sensitive electronics from ESD pulses, and high dielectric breakdown material can be added to prevent ESD pulse entry or exit of not otherwise protected circuit parts.

Abstract: In some examples, a stylus can include a conductive shield. In some examples, the conductive shield can include a plurality of traces along an edge of a printed circuit board (PCB) including the stylus circuitry. In some examples, the conductive shield can include a shield can coupled to the PCB and disposed around the components of the stylus circuitry. In some examples, the conductive shield can include a hollow portion into which the stylus circuitry can be disposed and a solid portion that can act as a reference electrode. In some examples, the conductive shield can include a hollow sleeve disposed around the stylus circuitry. In some examples, the conductive sleeve can be attached to the PCB. In some examples, the conductive sleeve can be disposed between layers of a housing of the stylus. In some examples, the conductive sleeve can be integrated with the stylus housing.

Abstract: The present invention is directed to a CH3-containing molecule comprising (a) a first polypeptide comprising a CH3 domain and a negatively charged domain comprising consecutive negatively charged amino acid residues; and (b) a second polypeptide comprising a CH3 domain and a positively charged domain comprising consecutive positively charged amino acid residues. Also provided are methods for conjugating synthetic molecules to a multi-specific antigen binding molecule.

Abstract: A module for a fire-mitigating surge arrester includes: an inner arrester module including an electrically conductive assembly; a cartridge including a first end, a second end, a sidewall that extends between the first end and the second end, and an exhaust region in the sidewall, the sidewall surrounding the inner arrester module; a first electrical component in contact with a first end of the electrically conductive assembly and the first end of the cartridge; a second electrical component in contact with a second end of the electrically conductive assembly and the second end of the cartridge; and a reinforcement structure on the sidewall, the first electrical component, and the second electrical component.

Abstract: A system may include a wearable electronic device that gathers force input. The device may transmit force measurement information and other input to external equipment such as a head-mounted device. The wearable electronic device may have a force sensor that gathers force measurements as the wearable electronic device is being worn. The force sensor may have a force sensor housing structure configured to form a fluid-filled channel and one or more collapsible force sensor elements such as collapsible fluid-filled domes or other protruding portions of the force sensor housing structure. A pressure sensor may monitor changes in pressure in a fluid that fills the channel and the fluid-filled domes. The monitored changes in pressure represent force measurements for force applied by a user's body part or other objects on the collapsible force sensor elements.

Abstract: In some implementations, a device may receive performance data associated with a performance of a user during an activity session. The device may receive, from a user device, media data associated with the user being involved in the activity session. The device may process, using a cognitive state analysis model, the media data to determine a cognitive state score associated with a cognitive state of the user in relation to the activity session. The device may process, using a performance analysis model, the cognitive state score and the performance data to generate a performance profile for the user. The device may determine, based on the performance profile, a recommendation associated with an activity performed during the activity session. The device may perform, based on the performance profile, an action associated with the recommendation.

Abstract: A system for producing fragmented video content is provided. The system includes a processing device, a non-transitory data store, and a memory storage device having at least a Manifest Processing Service (MPS) unit stored therein. The MPS unit is configured to generate at least one master manifest and store the master manifest in the non-transitory data store, the master manifest comprising a timing reference for a video fragment of the fragmented video content, wherein the timing reference indicates a temporal boundary of processing for the video fragment.

Abstract: A method for automatically filling a container with water and/or ice from a dispenser in a refrigerator door or within a refrigerator cavity includes the steps of: placing a container adjacent the dispenser; determining a volume of the container by using a camera associated with the dispenser, capturing a picture of the containing, visually estimating a volume of the container via a microprocessor associated with the camera or a cloud-based application associated with the camera, and converting the estimated container volume to an estimated weight based on densities of water and/or ice; filling the container with water and/or ice from the dispenser based on the estimated weight; and terminating filling of the container based on the estimated weight. And the refrigerator carrying out the method.

Abstract: Touch sensor panels (or touch screens) can improve signal-to-noise ratio (SNR) using touch electrode patterns for differential drive and/or differential sense techniques. In some examples, a touch sensor panel can include a two-dimensional array of touch nodes formed from a plurality of touch electrodes. Each column (or row) of touch nodes can be driven with a plurality of drive signals. For example, a first column (or row) of touch nodes can be driven by a first drive signal applied to one or more first touch nodes in the first column (or row) and a second drive signal applied to a one or more second touch nodes of the first column (or row). In some examples, the first drive signal and the second drive signal can be complimentary drive signals. In some examples, each row (or column) of touch electrodes can be sensed by differential sense circuitry.

Abstract: The present application discloses a multi-media interactive story-telling system that provides natural conversational interactions between a human user and a contributor who appears in multi-media recordings. The multi-media interactive story-telling system includes an intake device for recording interview sessions in which a contributor tells a life story about her life experience. The contributor may rely on a script when creating the life story. The multi-media interactive story-telling system further comprises storage devices for storing the recorded interview sessions and processors for organizing the recorded interview sessions. The multi-media interactive story-telling system further comprises an interactive device for retrieving and playing a recorded interview session in response to a query from a user.

Abstract: The present disclosure provides systems and methods for generating a digital menu for an establishment. In accordance with some embodiments, a system for generating a digital menu for an establishment is configured to: retrieve menu data from a point of sale (PoS) system at the establishment; retrieve supplemental information for the retrieved menu data; generate the digital menu based on the retrieved menu data and the supplemental information; and transmit the digital menu to an electronic device for display to a user.