Abstract: Embodiments of the present disclosure relate to a method of encrypting a secret storage structure. The method may include storing a secret in a secret storage structure. The secret storage structure may be encrypted by encrypting the secret using a wrap key that is generated based at least on a hardware-based root key and a first context. The secret storage structure may additionally be encrypted by encrypting the secret storage structure using an authentication key that is generated based at least on the hardware-based root key and a second context.

Abstract: The present disclosure provides systems and methods for disrupting the outer membrane of an oocyst in solution and delivering the solution to an animal. The system includes a vessel containing unbroken oocysts in solution, an oocyst processing chamber, and a delivery outlet. The unbroken oocysts are moved from the vessel through the processing chamber and a portion of the oocyst membranes are disrupted releasing sporocysts, the resulting solution is moved from the processing chamber into the delivery outlet where the solution is delivered to an animal. Methods of vaccination, including vaccination against an Eimeria infection, are also provided.

Abstract: Embodiments of the present disclosure relate to a method of encrypting a secret storage structure. The method may include storing a secret in a secret storage structure. The secret storage structure may be encrypted by encrypting the secret using a wrap key that is generated based at least on a hardware-based root key and a first context. The secret storage structure may additionally be encrypted by encrypting the secret storage structure using an authentication key that is generated based at least on the hardware-based root key and a second context.

Abstract: Embodiments of the present disclosure relate to a method of encrypting a secret storage structure. The method may include storing a secret in a secret storage structure. The secret storage structure may be encrypted by encrypting the secret using a wrap key that is generated based at least on a hardware-based root key and a first context. The secret storage structure may additionally be encrypted by encrypting the secret storage structure using an authentication key that is generated based at least on the hardware-based root key and a second context.

Abstract: A system for delivery of a fluid to an animal is provided in the present disclosure. The system includes a reservoir to hold a volume of fluid, delivery outlet, recirculating loop, and valve in fluid communication between the reservoir and delivery outlet, the valve having an inlet to receive fluid from the reservoir, a first outlet in fluid communication with the delivery outlet, and a second outlet in fluid communication with a return conduit to the reservoir. The system further includes control means for opening and closing the first and second valve outlets, and a pump to pump fluid from the reservoir to the valve and through the reservoir return conduit to the reservoir. When the control means opens the first outlet, the fluid flows from the reservoir to the delivery outlet. When the control means opens the second valve outlet, the fluid flows through the return conduit to the reservoir.

Abstract: The present disclosure provides systems and methods for disrupting the outer membrane of an oocyst in solution and delivering the solution to an animal. The system includes a vessel containing unbroken oocysts in solution, an oocyst processing chamber, and a delivery outlet. The unbroken oocysts are moved from the vessel through the processing chamber and a portion of the oocyst membranes are disrupted releasing sporocysts, the resulting solution is moved from the processing chamber into the delivery outlet where the solution is delivered to an animal. Methods of vaccination, including vaccination against an Eimeria infection, are also provided.

Abstract: A mobile waste processing system and method is disclosed for processing materials in a tank, typically an underground storage tank. A mobile fluid processing module is disposed adjacent to or over the tank, and sealingly connected to the tank through a tank riser. Support equipment, which may include an electric power module, a chiller module, a heating module, and a control and monitor module, are disposed a distance from the processing module, and connected thereto. A pump and optionally a dispersal nozzle are deployed from the processing module into the tank, and effluent is extracted for processing. The processing trailer includes effluent processing equipment such as an evaporator and demister, condenser, vacuum pump, and interim storage tank. The effluent is processed, and at least a portion is removed. Some of the processed effluent may be returned to the tank.

Abstract: A mobile waste processing system and method is disclosed for processing materials in a tank, typically an underground storage tank. A mobile fluid processing module is disposed adjacent to or over the tank, and sealingly connected to the tank through a tank riser. Support equipment, which may include an electric power module, a chiller module, a heating module, and a control and monitor module, are disposed a distance from the processing module, and connected thereto. A pump and optionally a dispersal nozzle are deployed from the processing module into the tank, and effluent is extracted for processing. The processing trailer includes effluent processing equipment such as an evaporator and demister, condenser, vacuum pump, and interim storage tank. The effluent is processed, and at least a portion is removed. Some of the processed effluent may be returned to the tank.

Abstract: A mobile waste processing system and method is disclosed for processing materials in a tank, typically an underground storage tank. A mobile fluid processing module is disposed adjacent to or over the tank, and sealingly connected to the tank through a tank riser. Support equipment, which may include an electric power module, a chiller module, a heating module, and a control and monitor module, are disposed a distance from the processing module, and connected thereto. A pump and optionally a dispersal nozzle are deployed from the processing module into the tank, and effluent is extracted for processing. The processing trailer includes effluent processing equipment such as an evaporator and demister, condenser, vacuum pump, and interim storage tank. The effluent is processed, and at least a portion is removed. Some of the processed effluent may be returned to the tank.

Abstract: A mobile waste processing system and method is disclosed for processing materials in a tank, typically an underground storage tank. A mobile fluid processing module is disposed adjacent to or over the tank, and sealingly connected to the tank through a tank riser. Support equipment, which may include an electric power module, a chiller module, a heating module, and a control and monitor module, are disposed a distance from the processing module, and connected thereto. A pump and optionally a dispersal nozzle are deployed from the processing module into the tank, and effluent is extracted for processing. The processing trailer includes effluent processing equipment such as an evaporator and demister, condenser, vacuum pump, and interim storage tank. The effluent is processed, and at least a portion is removed. Some of the processed effluent may be returned to the tank.

Abstract: The present invention provides improved methods of injecting an avian egg containing an embryo, preferably an early embryo (e.g., a blastoderm). The methods of the invention may be used to deliver a substance to an egg, remove a sample from an egg, and/or to insert a detector device into an egg to collect information therefrom. In preferred embodiments, the invention is used to deliver a substance to the embryo in ovo. In other preferred embodiments, the invention is used to produce chimeric or transgenic avian embryos in ovo.

Abstract: A scaled-up multi-coaxial fiber bioreactor, and variations of this bioreactor. The device is characterized by a hollow housing and an array of from about 20 to about 400 modules of hollow fibers, where each module includes at least three coaxial semipermeable hollow fibers. The innermost fiber provides a boundary for an innermost compartment which is connected to inlet and outlet ports. Arranged coaxially around the central hollow fiber are several other hollow fibers with their respective compartments, each compartment defined by a respective annular space between adjacent fibers and each including inlet and outlet ports. An outermost compartment for permitting integral aeration is the space between the outer side of the outermost fibers and the inner side of the housing, and has inlet and outlet ports. The hollow housing has inlet and outlet manifolds and flow distributors for each of the compartments. In a preferred embodiment the bioreactor is used as an extracorporeal liver.

Abstract: The present invention provides improved methods of injecting an avian egg containing an embryo, preferably an early embryo (e.g., a blastoderm). The methods of the invention may be used to deliver a substance to an egg, remove a sample from an egg, and/or to insert a detector device into an egg to collect information therefrom. In preferred embodiments, the invention is used to deliver a substance to the embryo in ovo. In other preferred embodiments, the invention is used to produce chimeric or transgenic avian embryos in ovo.

Abstract: An injection apparatus for injecting and/or removing substances from subjects, such as avian eggs, includes an injection needle having a body portion and a tapered portion that terminates at a tip. A tube is attached to and extends around the injection needle in substantially concentric relationship therewith. The tube has an end portion positioned a predetermined distance from the needle tip. The tube end portion acts as a stop to limit the distance the needle tip can be inserted into a subject, such as an egg, through a membrane of the subject. A detector may be utilized to measure force exerted on a membrane of an egg by a stop attached to a needle. Force exerted on a membrane by a needle and/or by a needle and a stop attached to the needle may also be measured.

Abstract: Methods an apparatus for accurately and reliably positioning a device within an egg are provided. An opening is formed within a portion of the shell of an egg and a device is extended through the opening. The device includes a needle having a lumen containing a fluid under pressure. Fluid pressure is monitored and, in response to detecting a change in pressure of the fluid, movement of the device is stopped. Alternatively, fluid flow is monitored and, in response to detecting fluid flow from the needle, movement of the device is stopped. The device may be a substance delivery device that is configured to deliver a substance into the subgerminal cavity of the egg. The device may be a substance removal device that is configured remove a substance from an egg.

Abstract: An injection apparatus for injecting and/or removing substances from subjects, such as avian eggs, includes an injection needle having a body portion and a tapered portion that terminates at a tip. A tube is attached to and extends around the injection needle in substantially concentric relationship therewith. The tube has an end portion positioned a predetermined distance from the needle tip. The tube end portion acts as a stop to limit the distance the needle tip can be inserted into a subject, such as an egg, through a membrane of the subject. A detector may be utilized to measure force exerted on a membrane of an egg by a stop attached to a needle. Force exerted on a membrane by a needle and/or by a needle and a stop attached to the needle may also be measured.

Abstract: An egg is accelerated such that the inner and outer shell membranes separate and allow the air cell to move within the egg when the egg is repositioned. By separating the outer and inner shell membranes of an egg, the air cell will reposition itself at the upper portion of the egg shell. An egg may be accelerated in various ways according to embodiments of the present invention including, but not limited to, agitation, “flicking” by hand, spinning, rotating, centrifuging, etc.

Abstract: The present invention provides improved methods of injecting an avian egg containing an embryo, preferably an early embryo (e.g., a blastoderm). The methods of the invention may be used to deliver a substance to an egg, remove a sample from an egg, and/or to insert a detector device into an egg to collect information therefrom. In preferred embodiments, the invention is used to deliver a substance to the embryo in ovo. In other preferred embodiments, the invention is used to produce chimeric or transgenic avian embryos in ovo.