Abstract: Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior to those of conjugates of spectrally similar dyes wherein attachment is at a different position. The invention includes derivative compounds having one or more benzo nitrogens.

Abstract: The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid.

Abstract: A manufacturing process produces a stabilizer bar having a thickened, reinforced portion formed by thermal spraying a molten metal onto a surface of the bar. The process produces a stabilizer bar having a thickened portion in the areas typically subject to fatigue failure while avoiding the addition of unnecessary material and weight to other locations of the stabilizer bar not needing reinforcement. In one example, the unprocessed bar stock is thermally sprayed in desired locations. The thermally sprayed bar is formed into a desired shape and heat treated. The heat treated, formed, and sprayed bar is shot peened. Alternatively, the bar is thermally sprayed after the surface has been prepared by shot peening. Forming of the bar may also occur before thermal spraying, and pretreated bar stock may also be used.

Abstract: Cyanine dye compounds having a negatively charged substituent that are nucleic acid stains, particularly for fluorescent staining of DNA, including compounds having the formula wherein W forms one or two fused 5- or 6-membered aromatic rings, a has a value of 0 or 1, n has a value of 0, or 1, X is O, S, or Se, and D is a pyridinium, or quinolinium moiety, provided that the compound has at least one negatively charged substituent.

Abstract: Cyanine dye compounds having a substituted methine moiety that are nucleic acid stains, particularly for fluorescent staining of RNA, including compounds having the formula where R1 is a C1-C6 alkyl, sulfoalkyl, carboxyalkyl or C1-C6 alkoxy; each R2 is independently selected from the group consisting of H, C1-C6 alkyl, C1-C6 alkoxy, fused benzo, trifluoromethyl, amino, sulfo, carboxy and halogen, that is optionally further substituted; at least one of R3, R4, and R5 is an alkyl, aryl, heteroaryl, cyclic, or heterocyclic moiety that is optionally substituted by alkyl, amino, aminoalkyl, carboxy, nitro, or halogen; and the remaining R3, R4 or R5 are hydrogen; X is S, O, or Se; and D is a substituted or unsubstituted pyridinium, quinolinium or benzazolium moiety.

Abstract: The present invention provides methods and non-fluorescent carbocyanine quencher compounds having the general formula: Wherein the A moiety is a substituted pyridinium, unsubstituted pyridinium, substituted quinolinium, unsubstituted quinolinium, substituted benzazolium, unsubstituted benzazolium, substituted indolinium, or substituted indolinium. The invention further provides luminescent donor molecule-quencher pairs and luminescent donor molecule-quencher-luminescent acceptor molecule conjugates wherein the quencher is a cyanine compound of the present invention. The energy transfer pairs are used to detect an analyte of interest in a sample.

Abstract: An anti-shift collar prevents lateral movement of a stabilizer bar assembly. The anti-shift collar is thermally formed to a central segment of the stabilizer bar and is locked to the central segment thereby.

Abstract: Embodiments of the present invention provide methods and nucleic acid reporter molecules for the detection of nucleic acid in a sample. The nucleic acid reporter molecule comprises two unsymmetrical cyanine monomer moieties, which may be the same or different, that are covalently attached by a linker comprising at least one aromatic, heteroaromatic, cyclic or heterocyclic moiety comprising 3-20 non-hydrogen atoms selected from the group consisting of O, N, S, P and C. The linker may be rigid, relatively flexible or some degree thereof. The unsymmetrical cyanine monomer moieties comprise a substituted or unsubstituted benzazolium moiety and a substituted or unsubstituted pyridinium or quinolinium moiety that is connected by a methine bridge that is monomethine, trimethine or pentamethine. The linkers form the cyanine dimer compounds by attaching to the pyridinium or quinolinium moiety of the monomer moieties.

Abstract: Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior to those of conjugates of spectrally similar dyes wherein attachment is at a different position. The invention includes derivative compounds having one or more benzo nitrogens.

Abstract: Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior to those of conjugates of spectrally similar dyes wherein attachment is at a different position. The invention includes derivative compounds having one or more benzo nitrogens.

Abstract: Seamless steel tubes suitable for use as grades of casing and line pipe having yield strengths in excess of 70,000 psi, without being heat treated, are made of an alloy comprising, by weight, about 0.10% to 0.18% carbon, about 1.0% to 2.0% manganese, about 0.10% to 0.16% vanadium, about 0.008% to 0.012% titanium and about 150 parts per million to 220 parts per million nitrogen, the balance comprising iron and incidental impurities. Strains are applied to the shell in a stretch reducing mill below the T.sub.nr of the steel and above the A.sub.r3 to provoke dynamic recrystallization. The nitrogen and vanadium are preferably introduced to the steel during alloying in the form of a VN alloying agent. The vanadium, titanium and nitrogen are predominantly present as vanadium nitride and titanium nitride. The steel may also comprise 0.03% to 0.05% aluminum by weight.

Abstract: The strength of interstitial free steels is increased by up to 100% and the ductile to brittle transition temperature is decreased by up to 100.degree. C. by warm finish rolling in the single phase ferrite region below A.sub.rl to effect ferrite dynamic recrystallization of the steel microstructure to a ferrite structure of grain size having a grain size of up to 5 .mu.m, and especially an ultra fine grain size of 1 to 2 .mu.m; the method may be employed in various hot working methods including strip and rod mills, planetary hot rolling and extrusion.

Abstract: A method for producing seamless steel tubes suitable for use as grades of casing and line pipe having yield strengths in excess of 70,000 psi, without being heat treated. Such steels are made of an alloy comprising, by weight, about 0.10% to 0.18% carbon, about 0.10% to 2.0% manganese, about 0.10% to 0.16% vanadium, about 0.008% to 0.012% titanium and about 150 parts per million to 220 parts per million nitrogen, the balance comprising iron and incidental impurities. The subject method comprises the steps of alloying a steel of the aforesaid chemical composition, forming the steel into a billet of steel, reheating the billet in a reheating furnace, passing the billet through a piercing mill to form a steel shell, elongating the steel shell within a mandrel mill, and reducing the diameter of the elongated shell in a stretch reducing mill. Strains are applied to the shell in the stretch reducing mill below the T.sub.nr of the steel and above the A.sub.r3 to provoke dynamic recrystallization.

Abstract: A series of thermomechanical workings such as temperature-controlled torsional strains are applied to a specimen of steel at strain and temperature levels and interpass times selected to simulate rolling mill conditions. The measured stress values are compared with the temperatures of the steel during the working periods during which the respective values were obtained. Thermomechanical working schedules are repeated at selected varying starting and terminating temperatures thereby to obtain a series of possible rolling schedules. These simulations are selected so that a varying number of reduction passes in the sequence occur at steel temperatures below temperature A.sub.r3. The value of a selected parameter of the worked steel, e.g. yield strength, is measured at ambient temperature.