Abstract: A method for testing a microfluidic device includes interfacing a microfluidic device to a fluidic parameter testing system. The microfluidic device has an internal rotary valve and internal fluidic channels. Each channel has a port with a predetermined port position that the rotary valve is to align to in order to select any one of a plurality of reagents which flow through the channels. The rotary valve is rotated via the testing system to a plurality of rotary valve position of the rotary valve. A fluidic parameter of the microfluidic device is measured at each rotary valve position. The fluidic parameter is mapped relative to the rotary valve positions. It is determined from the mapping if the rotary valve aligns with each of the predetermined port positions for a flow of the reagents through the channels.

Abstract: A system includes a fluidic device, a flow control valve, a first reagent fluid reservoir fluidly connectable to the fluidic device by the flow control valve, a first fluid buffer reservoir fluidly connectable to the fluidic device by the flow control valve, and a common fluid buffer source fluidly connectable to the fluidic device by the flow control valve. The flow control valve permits flow comprising: (i) flow from the first reagent fluid reservoir to the fluidic device, (ii) flow from the common fluid buffer source to the fluidic device, (iii) flow from the fluidic device to the first fluid buffer reservoir, (iv) flow from the first reagent fluid reservoir to the fluidic device, and (v) flow from the first fluid buffer reservoir to the fluidic device.

Abstract: Systems and methods for operating an engine and a torque converter are presented. In one example, slip of a torque converter is adjusted via at least partially closing or opening a torque converter clutch in response to vehicle vibration. The vehicle vibration may be based on road surface conditions and an actual total number of operating cylinders of the engine.

Abstract: Disclosed herein are devices, systems and methods for conducting a reaction using electrowetting in a vertical or substantially vertical position. Some embodiments disclosed herein provide fluidic cartridges for use in a substantially vertical position comprising: (a) a front substrate; (b) a back substrate; (c) a droplet operations gap formed between the front substrate and the back substrate; and (d) a plurality of electrodes on the front substrate or the back substrate, wherein the plurality of electrodes are configured to transport a droplet along a substantially vertical plane defined by the front substrate and the back substrate.

Abstract: The present disclosure provides compositions and methods of site-specific modification of a target DNA, or a protein associated with a target DNA, in a eukaryotic cell. The present disclosure provides methods of binding a target DNA in a eukaryotic cell.

Abstract: Systems and methods for operating an engine and a torque converter are presented. In one example, slip of a torque converter is adjusted via at least partially closing or opening a torque converter clutch in response to vehicle vibration. The vehicle vibration may be based on road surface conditions and an actual total number of operating cylinders of the engine.

Abstract: Methods and compositions are provided for highly efficient delivery of Cas9 and Cas9 ribonucleoproteins to cells, including primary hematopoietic cells and primary hematopoietic stem cells.

Abstract: An input is received from a user selecting one or more of a plurality of icons on a non-wearable user device display. A wearable device is instructed to display the selected icons on a wearable device display. A vehicle component is actuated based on a second input selecting one of the icons displayed on the wearable device display.

Abstract: An online social networking system collects data relating to members, and clusters the data into a plurality of clusters. The system identifies anomalous activity by a member in a cluster, and predicts an update to a profile of the member based on the identified anomalous activity of the member. The system presents to the member a proposed update to the profile of the member based on the prediction, receives input from the member in response to the proposed update, and updates the profile of the member based on the input received from the member.

Abstract: A cartridge assembly comprises a housing having an illumination chamber and a well plate. The well plate is maintained within the housing and has liquid wells to receive desired amounts of liquids. The well plate includes a fluidics analysis station aligned with the illumination chamber, and an interface window and interface ports located at the fluidics analysis station. The well plate includes a valve station and pump station. A piercer unit is provided in the housing and positioned proximate to the wells. The piercer unit includes a piercer element and is to be moved to a piercing position where the piercer element pierces a cover for the corresponding well. A pump assembly on the well plate at the pump station manages fluid flow through the channels between the pump station and the fluidics analysis station. The housing includes a flow cell chamber to receive a removable flow cell cartridge.

Abstract: A cartridge assembly that comprises a housing, including a flow cell chamber to receive a flow cell, and a well plate having liquid wells to receive desired amounts of liquids. The well plate includes a valve station, a pump station and a fluidics analysis station, and channels associated therewith. A pump assembly to manage fluid flow through the channels between the pump station and the fluidics analysis station. A rotary valve assembly that includes a rotor shaft and rotor valve positioned to rotate about a rotational axis and to selectively couple the wells to the pump station. The rotor shaft includes a dual spline configuration at the distal end thereof. The dual spline configuration has first and second sets of splines. The first set of splines forms a drive interface and the second set of splines forms a position encoding interface.

Abstract: A system includes a processor configured to receive a user-selected vehicle-detectable state and corresponding user-defined state-variable value. The processor is also configured to receive a user-defined vehicle-enactable action. The processor is further configured to save the state-variable value as a condition for enacting the vehicle-enactable action saved in conjunction with the state-variable value. The processor is additionally configured to monitor the vehicle-detectable state and enact the vehicle-enactable action upon determining via the monitoring that the vehicle-detectable state corresponds to the user-defined state-variable value saved in conjunction with the state-variable value.

Abstract: Systems and methods for operating an engine and a torque converter are presented. In one example, slip of a torque converter is adjusted via at least partially closing or opening a torque converter clutch in response to vehicle vibration. The vehicle vibration may be based on road surface conditions and an actual total number of operating cylinders of the engine.

Abstract: Systems and methods for operating an engine in a variety of different cylinder operating modes are presented. In one example, an actual total number of available cylinder modes is increased in response to a vehicle's suspension setting and road roughness. By increasing the available cylinder modes, the engine may be operated in a higher number of modes where one or more engine cylinders may be deactivated to conserve fuel. The number of cylinder modes is increased during conditions where vehicle occupants may be less likely to object to operating the engine with fewer active cylinders.

Abstract: A cartridge assembly that comprises a housing, including a flow cell chamber to receive a flow cell, and a well plate having liquid wells to receive desired amounts of liquids. The well plate includes a valve station, a pump station and a fluidics analysis station, and channels associated therewith. A pump assembly to manage fluid flow through the channels between the pump station and the fluidics analysis station. A rotary valve assembly that includes a rotor shaft and rotor valve positioned to rotate about a rotational axis and to selectively couple the wells to the pump station. The rotor shaft includes a dual spline configuration at the distal end thereof. The dual spline configuration has first and second sets of splines. The first set of splines forms a drive interface and the second set of splines forms a position encoding interface.

Abstract: Disclosed herein are devices, systems and methods for conducting a reaction using electrowetting in a vertical or substantially vertical position. Some embodiments disclosed herein provide fluidic cartridges for use in a substantially vertical position comprising: (a) a front substrate; (b) a back substrate; (c) a droplet operations gap formed between the front substrate and the back substrate; and (d) a plurality of electrodes on the front substrate or the back substrate, wherein the plurality of electrodes are configured to transport a droplet along a substantially vertical plane defined by the front substrate and the back substrate.

Abstract: This invention relates to clear, semicrystalline, strain induced crystallized polyester films heat laminated onto metal substrates. The films contain at least one polyester which comprises at least of one or more monomers selected from 1,4-cyclohexanedimethanol or 2,2,4,4-tetramethyl-1,3-cyclobutanediol. The articles of the present invention exhibit enhanced mechanical properties useful for the fabrication of thin metal articles such as metal cans.

Abstract: Systems and methods for operating an engine in a variety of different cylinder operating modes are presented. In one example, an actual total number of available cylinder modes is increased in response to a vehicle's suspension setting and road roughness. By increasing the available cylinder modes, the engine may be operated in a higher number of modes where one or more engine cylinders may be deactivated to conserve fuel. The number of cylinder modes is increased during conditions where vehicle occupants may be less likely to object to operating the engine with fewer active cylinders.

Abstract: The present disclosure provides compositions and methods of site-specific modification of a target DNA, or a protein associated with a target DNA, in a eukaryotic cell. The present disclosure provides methods of binding a target DNA in a eukaryotic cell.

Abstract: The present invention provides methods, compositions, and systems for distributing molecules and complexes into reaction sites. In particular, the methods, compositions, and systems of the present invention result in an active loading of molecules and complexes into reaction sites with improved efficiency over loading by passive diffusion methods alone.