Abstract: The present invention provides for a novel recombinant or isolated nucleic acid useful for integrating or being maintained in an Archaea or acidophilic hyperthermophilic eubacteria. The nucleic acid encodes a nucleotide sequence that is capable of stably integrating into the chromosome of a host cell, or being maintained as an extrachromosomal element in a host cell, that is an Archea, and a nucleotide sequence of interest. The present invention also provides for an Archaea host cell comprising the nucleic acid stably integrated into the chromosome or maintained episomally in the host cell, and a method of expressing the nucleotide sequence of interest in the host cell and/or directing glycosylation, multimerization, and/or membrane association or integration.

Abstract: The present disclosure relates to a composition for cleaning or sanitizing a surface, methods of using the same for cleaning and sanitizing a surface, and kits comprising the same. The composition includes an enzyme isolated from a hyperthermophilic and/or acidophilic organism and optionally, an acid and an additive. The composition can be used at temperatures ranging from about 50° C. to 110° C., preferably at temperatures ranging from about 70° C. to 100° C. In addition, the composition can be used at pH values ranging from 0.5 to 7, preferably at pH values ranging from 2 to 5.

Abstract: The present invention provides for a novel recombinant or isolated nucleic acid useful for integrating or being maintained in an Archaea or acidophilic hyperthermophilic eubacteria. The nucleic acid encodes a nucleotide sequence that is capable of stably integrating into the chromosome of a host cell, or being maintained as an extrachromosomal element in a host cell, that is an Archea, and a nucleotide sequence of interest. The present invention also provides for an Archaea host cell comprising the nucleic acid stably integrated into the chromosome or maintained episomally in the host cell, and a method of expressing the nucleotide sequence of interest in the host cell and/or directing glycosylation, multimerization, and/or membrane association or integration.

Abstract: The present invention provides for a novel recombinant or isolated nucleic acid useful for integrating or being maintained in an Archaea or acidophilic hyperthermophilic eubacteria. The nucleic acid encodes a nucleotide sequence that is capable of stably integrating into the chromosome of a host cell, or being maintained as an extrachromosomal element in a host cell, that is an Archea, and a nucleotide sequence of interest. The present invention also provides for an Archaea host cell comprising the nucleic acid stably integrated into the chromosome or maintained episomally in the host cell, and a method of expressing the nucleotide sequence of interest in the host cell and/or directing glycosylation, multimerization, and/or membrane association or integration.

Abstract: Kits, methods and systems for providing a service to provide a subject with information regarding the state of a subject's DNA damage. Collection, processing and analysis of samples are also described.

Abstract: Kits, methods and systems for providing a service to provide a subject with information regarding the state of a subject's DNA damage. Collection, processing and analysis of samples are also described.

Abstract: The present invention provides for a novel recombinant or isolated nucleic acid useful for integrating or being maintained in an Archaea or acidophilic hyperthermophilic eubacteria. The nucleic acid encodes a nucleotide sequence that is capable of stably integrating into the chromosome of a host cell, or being maintained as an extrachromosomal element in a host cell, that is an Archea, and a nucleotide sequence of interest. The present invention also provides for an Archaea host cell comprising the nucleic acid stably integrated into the chromosome or maintained episomally in the host cell, and a method of expressing the nucleotide sequence of interest in the host cell and/or directing glycosylation, multimerization, and/or membrane association or integration.

Abstract: Systems and methods for determining the sensitivity of cells (and/or a subject) to ionizing radiation are provided. The systems can comprise a microfluidic device comprising a plurality of microfluidic cavities each configured to contain cells; a source of ionizing radiation configured to deliver ionizing radiation to cells in the microfluidic cavities; and an imaging system configured to detect radiation-induced foci in cells when they are disposed in the microfluidic cavities. The methods can involve contacting a biological sample comprising cells from a subject with ionizing radiation; detecting and quantifying radiation induced foci in the cells at least two different time points; and determining a repair kinetic for radiation induced foci that is a measure of the rate of disappearance of the foci. Methodologies are also provided for in-home blood collection and fixation of nucleated blood cells in a manner to preserve health and fitness biomarkers inherent to these cells.