Abstract: Synthetic representative HCV subtypes, including a 1a and 1b genome, dubbed Bole1a and Bole1b, are provided using an inventive method of Bayesian phylogenetic tree analysis, ancestral sequence reconstruction and covariance analysis. Bole1a branches centrally among 390 full-genome sequences used in its design, a carefully curated 143 sequence full-genome dataset, and separate genomic regions including an independent set of 214 E1E2 sequences from a Baltimore cohort. Bole1a is phylogenetically representative of widely circulating strains. Full genome non-synonymous diversity comparison and 9-mer peptide coverage analysis showed that Bole1a is able to provide more coverage (94% and 78% respectively) than any other sequence in the dataset including H77, a traditional reference sequence. Bole1a also provides unsurpassed epitope coverage when compared to all known T cell epitopes.

Abstract: The present invention relates in part to devices, systems, and methods for the production and characterization of engineered tissue constructs. The devices and methods generate decellularized tissue constructs that can be recellularized using donor cells for high-throughput studies of organ physiology, and/or organ pathology. The devices and methods can be used with the systems to mechanically and electrically stimulate the engineered tissue constructs under culture or physiological conditions to detect and assess the presence and degree of any organ pathologies.

Abstract: The invention relates to a method of automatic dishwashing in a dishwasher having a wash tank, comprising: in a first step, delivering a first composition to dishware in the tank in the form of a mist; and afterwards, in a second step, delivering a second composition to the dishware in the tank in the form of a jet; wherein the first and second compositions are different, the first composition comprises a bleach, and the second composition is alkaline and comprises a builder. The invention also provides a dishwasher adapted to carry out the inventive method.

Abstract: The invention relates to a method of automatic dishwashing of dishware using wash water, in which, in a first step, a first composition, which comprises an oxygen bleach but substantially no enzyme, is supplied to the wash water, and the dishware is washed in a washing zone with the oxygen bleach-containing wash water; and, in a second step which occurs after the first step, a second composition, which comprises an enzyme but substantially no bleach, is supplied to the wash water, and the dishware is washed in said washing zone with the enzyme-containing wash water. The invention also relates to an automatic dishwasher and a cartridge suitable for use in this method.

Abstract: Synthetic representative HCV subtypes, including a 1a and 1b genome, dubbed Bole1a and Bole1b, are provided using an inventive method of Bayesian phylogenetic tree analysis, ancestral sequence reconstruction and covariance analysis. Bole1a branches centrally among 390 full-genome sequences used in its design, a carefully curated 143 sequence full-genome dataset, and separate genomic regions including an independent set of 214 E1E2 sequences from a Baltimore cohort. Bole1a is phylogenetically representative of widely circulating strains. Full genome non-synonymous diversity comparison and 9-mer peptide coverage analysis showed that Bole1a is able to provide more coverage (94% and 78% respectively) than any other sequence in the dataset including H77, a traditional reference sequence. Bole1a also provides unsurpassed epitope coverage when compared to all known T cell epitopes.

Abstract: Synthetic representative HCV subtypes, including a 1a and 1b genome, dubbed Bole1a and Bole1b, are provided using an inventive method of Bayesian phylogenetic tree analysis, ancestral sequence reconstruction and covariance analysis. Bole1a branches centrally among 390 full-genome sequences used in its design, a carefully curated 143 sequence full-genome dataset, and separate genomic regions including an independent set of 214 E1E2 sequences from a Baltimore cohort. Bole1a is phylogenetically representative of widely circulating strains. Full genome non-synonymous diversity comparison and 9-mer peptide coverage analysis showed that Bole1a is able to provide more coverage (94% and 78% respectively) than any other sequence in the dataset including H77, a traditional reference sequence. Bole1a also provides unsurpassed epitope coverage when compared to all known T cell epitopes.

Abstract: A mouthpiece (10) for an inhaler or inhalation device (100) includes an elongated body (12) having a substantially oval opening (14) at a first end and a substantially round opening (18) at a second end opposite from the first end, a plurality of depending arms (24), extending from the elongated body forming at least one air passageway (26) between two depending arms (24), and at least one flexible gripping finger (32) coupled to an inner surface of each depending arm (24), extending inwardly into the medicament delivery passageway of the mouthpiece at or near the substantially round opening (18) at the opposite end. Once coupled with an inhaler or inhalation device, the mouthpiece functions as a “pass through” mouthpiece to improve the consistency of inhaled medicament/drug delivery.

Abstract: The present invention provides a non-aqueous liquid, gel or paste composition, comprising a colourant and methylglycine diacetic acid (MGDA) or a salt thereof. The compositions afford enhanced stability of the colourant in the presence of MGDA. The invention also provides a unit dose detergent product comprising the inventive composition, a washing or cleaning process that utilizes this composition or product, and the use of this composition or product for washing or cleaning.

Abstract: The invention relates to detergent compositions comprising granular non-phosphate builder. The granules comprise an amino acid based builder, preferably MGDA, and s silicate. The composition is housed in a rigid PVOH capsule.

Abstract: Synthetic representative HCV subtypes, including a 1a and 1b genome, dubbed Bole1a and Bole1b, are provided using an inventive method of Bayesian phylogenetic tree analysis, ancestral sequence reconstruction and covariance analysis. Bole1a branches centrally among 390 full-genome sequences used in its design, a carefully curated 143 sequence full-genome dataset, and separate genomic regions including an independent set of 214 E1E2 sequences from a Baltimore cohort. Bole1a is phylogenetically representative of widely circulating strains. Full genome non-synonymous diversity comparison and 9-mer peptide coverage analysis showed that Bole1a is able to provide more coverage (94% and 78% respectively) than any other sequence in the dataset including H77, a traditional reference sequence. Bole1a also provides unsurpassed epitope coverage when compared to all known T cell epitopes.

Abstract: A lumbar extension machine is disclosed including a seat with an upright rotatable back member securable to a variable resistance module or statically secured to a load cell module. A lower body system is provide to optimize the exercise of the lower back by preventing movement and work by the hips and thighs. A pelvic restraint pad is located adjacent the seat and the seat is adjustable at the pad to insure that the iliac crest is properly located at the pad. An adjustable foot rest is provided to align the thigh with the seat angle after the seat height has been properly adjusted. The load cell provides strength testing to allow the clinician to determine relative strength and establish treatment parameters and monitor treatment progress and validate and quantify treatment results.

Abstract: A telecommunications network uses a supervisory service broker in a packet messaging environment, particularly Session Initiation Protocol (SIP) messaging, to changeably allocate and to account for the usage of network resources by users. The SIP INVITE and other messages are passed through the supervisory broker, monitored for accounting and potentially redirected to change allocations of resources. The resources can generally comprise applications and applications servers such as media storage and playback, voicemail and directory services, subnet messaging and instant messaging, voice-over-Internet, wireless linkages and others, and can be logically grouped in packages that are allocable. Users such as telecom carriers and others can contract for usage and also can contract to provide resources for use by others, in an unlimited way or according to terms, and for appropriate credit.

Abstract: A telecommunications network uses a supervisory service broker in a packet messaging environment, particularly Session Initiation Protocol (SIP) messaging, to changeably allocate and to account for the usage of network resources by users. The SIP INVITE and other messages are passed through the supervisory broker, monitored for accounting and potentially redirected to change allocations of resources. The resources can generally comprise applications and applications servers such as media storage and playback, voicemail and directory services, subnet messaging and instant messaging, voice-over-Internet, wireless linkages and others, and can be logically grouped in packages that are allocable. Users such as telecom carriers and others can contract for usage and also can contract to provide resources for use by others, in an unlimited way or according to terms, and for appropriate credit.