Abstract: The method includes obtaining input data representative of a first parameter of a cryogenic medium in a cryogenic cooling system is provided. The method further includes calculating an expected value representative of a second parameter of the cryogenic medium in the cooling system via a predefined physics based model. A second input data representative of the second parameter of the cryogenic medium in the cooling system is estimated. The expected value is compared to the second input data and difference between the expected value and the second input data is correlated to statistical data to estimate a likelihood of cryogenic cooling system event.

Abstract: A method and apparatus for the collection of physiological data from a patient is disclosed herein. An electrode assembly comprises an external label identifying an anatomical location and an electrode identifying circuitry that produces a signal indicative of the anatomical location to which the electrode assembly is to be attached. The electrode assembly transmits both the collected physiological signal and the identification signal to a data monitor for collection and processing physiological data.

Abstract: A multiple-beam antenna system includes at least one reflector and a cluster of horns for feeding the reflector. A horn of the cluster of horns is configured for providing transmission and reception of signals over respective transmission and reception frequency bands. The horn includes a substantially conical wall having an internal surface with a variable slope. The internal surface of the substantially conical wall includes slope discontinuities. At least one of the slope discontinuities has a diameter greater than 1.7 times the wavelength of the lowest frequency of the transmission frequency band. The diameter is also greater than 1.7 times the wavelength of the highest frequency of the transmission frequency band. In addition, the diameter is greater than 1.7 times the wavelength of the lowest frequency of the reception frequency band, and the diameter is greater than 1.7 times the wavelength of the highest frequency of the reception frequency band.

Abstract: Systems and methods for steam turbine remote monitoring, calculating corrected efficiency, monitoring performance degradation, diagnosing and benchmarking are disclosed with an example turbine system including a turbine, a data acquisition device coupled to the turbine, the data acquisition device for collecting turbine data that includes performance parameters of the turbine and a central monitoring system coupled to the data acquisition device, the central monitoring system for receiving the collected turbine data and processing the turbine data to determine turbine performance.

Abstract: Antenna system and methodology for producing multiple interleaved beams using a hybrid frequency-reuse scheme. In particular, depending on the traffic demand in specific coverage areas, some beams are assigned with frequency channels in accordance with a 4-cell frequency-reuse scheme, and the other beams are assigned with frequency channels in accordance with a 7-cell frequency-reuse scheme. The antenna system has multiple feeds divided into clusters, and a number of reflectors fed by respective feed clusters and configured to form one beam for each of the feeds.

Abstract: Disclosed is a process for separating and/or purifying a nucleic acid by elution of the nucleic acid from anion exchange resins under conditions of high salt concentration and the presence in the eluant of an additive comprising guanidine, or a guanidine-like derivative. The process allows high recovery of nucleic acids from anion exchange resins without impairing the nucleic acid stability as compared with conventional ion exchange chromatographic procedures.

Abstract: The invention provides an improved method for the purification of nucleic acid molecules, which method comprises generating a cellular lysate containing the nucleic acid; contacting the lysate with an anion exchanger bound to a solid support matrix under conditions such that the anion exchanger binds the nucleic acid; followed by eluting the nucleic acid from the anion exchanger with an aqueous mobile phase comprising an elution solution; and desalting the eluted nucleic acid such that it is suitable for downstream applications. The improvement of the method includes providing the anion exchanger in a packed column, wherein the column is packed using a salt solution containing an antimicrobial agent. In addition, the salt solution has a salt concentration similar to that of the lysate, such that the column does not need equilibration prior to sample loading.

Abstract: An antenna system for generating and configuring at least one defocused beam is provided. The antenna system includes a reflector having a focal plane and a non-parabolic curvature for forming the at least one defocused beam, and a plurality of feed antennas that illuminate the reflector. Each feed antenna is disposed in the focal plane of the reflector. The antenna system further includes at least one incoming signal dividing network that divides at least one incoming signal into a plurality of sub-signals, each corresponding to one of the feed antennas, a plurality of variable phase shifters, each receiving one of the sub-signals from the incoming signal dividing network and phase shifting the sub-signal to generate a corresponding phase-shifted sub-signal, and a plurality of fixed-amplitude amplifiers, at least one corresponding to each of the feed antennas.

Abstract: An antenna system includes a reflector having a modified-paraboloid shape; and a multi-beam, multi-band feed array located at a focal point of the reflector so that the antenna system forms a multiple congruent beams that are contiguous. The system has a single reflector with non-frequency selective surface. The reflector is sized to produce a required beam size at K-band frequencies and is oversized at EHF-band frequencies. The synthesized reflector surface is moderately shaped and disproportionately broadens EHF-band and Ka-band beams compared to K-band beams. The synthesized reflector surface forms multiple beams each having a 0.5-degree diameter at K-band, Ka-band, and EHF band. The multi-beam, multi-band feed array includes a number of high-efficiency, multi-mode circular horns that operate in focused mode at K-band and defocused mode at Ka-band and EHF-band by employing “frequency-dependent” design for the horns.

Abstract: Oligonucleotide tagged nucleoside triphosphates, OTNTPs, which are substrates for polymerases and or terminal nucleotidyl transferases are provided as well as methods of making these OTNTPs. Further provided are OTNTPs with fluorescent dyes including energy transfer dyes, attached to the oligonucleotide chain, OTNTPs with unnatural bases incorporated in the oligonucleotide sequence and methods for incorporating these OTNTPs in DNA or RNA. Also provided are methods for using the oligonucleotides on OTNTPs for amplifying the oligo sequence on the OTNTP using an amplification method described above. Further provided are reactive bifunctional amidites, methods of making these compounds and methods for detecting single nucleotide polymorphisms using the above OTNTPs. Methods for detecting differential gene expression using the OTNTPs and methods of separating specifically modified DNA or RNA using the OTNTPs are also provided.

Abstract: A dual-band multiple beam antenna system for a communications satellite sharing a set of reflector antennas for the transmit and receive frequencies. One set of reflectors is common to both the downlink and uplink frequencies. The feed horns are diplexed and exhibit frequency-dependent radiation patterns that separate the phase centers over the downlink and uplink frequency bands to obtain dual-band performance. The focal point of the reflector is in close proximity to the phase center corresponding to the downlink frequency band. The phase center for the uplink frequency band is spaced a predetermined distance from the phase center of the downlink frequency band. According to the present invention, the uplink frequencies are defocused and the downlink frequencies are focused thereby creating identical radiation patterns at both frequency bands and over the coverage region of the communications satellite.

Abstract: The present invention relates to a novel set of four fluorescently labeled dye terminators. Two of them are single dye labeled terminators and the other two dye terminators are based on fluorescent resonance energy transfer (FRET). The FRET dye terminators are generated from the 4?,5?-bis-aminomethylfluorescein. Of the two amino groups of the donor dye, 4?,5?-bis-aminomethylfluorescein, one amino group is used to attach the acceptor dye, and the other amino group is used to attach the dideoxynucleoside-5?-triphosphate. Their preparation, energy transfer efficiency, and use as labels, specifically, in DNA sequencing reactions is disclosed.

Abstract: An antenna comprising a reflector having a reflector surface profile for reflecting a plurality of signals comprising a plurality of communication bands; a multi-depth corrugated horn assembly for receiving the signal comprising the plurality of communication bands; a stepped waveguide coupled to the corrugated horn; a first polarizer coupled to the stepped waveguide for separating a first communication band from the plurality of communication bands; a second polarizer coupled to the stepped waveguide for separating a second communication band from the plurality of communication bands; and a third polarizer coupled to the stepped waveguide for separating a third communication band from the plurality of communication bands. The antenna can simultaneously operate in the K, Ka and EHF frequency bands. Extension of this antenna to include five separate frequency bands is demonstrated.

Abstract: An antenna system includes a reflector having a modified-paraboloid shape; and a multi-beam, multi-band feed array located at a focal point of the reflector so that the antenna system forms a multiple congruent beams that are contiguous. The system has a single reflector with non-frequency selective surface. The reflector is sized to produce a required beam size at K-band frequencies and is oversized at EHF-band frequencies. The synthesized reflector surface is moderately shaped and disproportionately broadens EHF-band and Ka-band beams compared to K-band beams. The synthesized reflector surface forms multiple beams each having a 0.5-degree diameter at K-band, Ka-band, and EHF band. The multi-beam, multi-band feed array includes a number of high-efficiency, multi-mode circular horns that operate in focused mode at K-band and defocused mode at Ka-band and EHF-band by employing “frequency-dependent” design for the horns.

Abstract: Exploitation of suitably functionalized heterocyclic molecules, in the design and synthesis of Fluorescence Resonance Energy Transfer (FRET) cassettes and their corresponding dideoxynucleotide terminators culminated into efficient reagents for DNA sequencing. Additionally, these FRET cassettes/terminators, of the present invention, derived from different classes of heterocyclic systems have high potential to be used for general labelling of biological molecules to generate highly sensitized signals. Their preparation, energy transfer efficiency, and use as labels, specifically, in DNA sequencing reactions is disclosed.

Abstract: Exploitation of suitably functionalized heterocyclic molecules, in the design and synthesis of Fluorescence Resonance Energy Transfer (FRET) cassettes and their corresponding dideoxynucleotide terminators culminated into efficient reagents for DNA sequencing. Additionally, these FRET cassettes/terminators, of the present invention, derived from different classes of heterocyclic systems have high potential to be used for general labelling of biological molecules to generate highly sensitized signals. Their preparation, energy transfer efficiency, and use as labels, specifically, in DNA sequencing reactions is disclosed.

Abstract: In one embodiment of a networking module, a first block receives a serial digital media signal, and provides a parallel digital media signal based on the serial digital media signal. A second block, operative with the first block, stores the parallel digital media signal in a corresponding slot in an outgoing frame, and sends the outgoing frame in response to receiving an incoming frame.

Abstract: Oligonucleotide tagged nucleoside triphosphates, OTNTPs, which are substrates for polymerases and or terminal nucleotidyl transferases are provided as well as methods of making these OTNTPs. Further provided are OTNTPs with fluorescent dyes including energy transfer dyes, attached to the oligonucleotide chain, OTNTPs with unnatural bases incorporated in the oligonucleotide sequence and methods for incorporating these OTNTPs in DNA or RNA. Also provided are methods for using the oligonucleotides on OTNTPs for amplifying the oligo sequence on the OTNTP using an amplification method described above. Further provided are reactive bifunctional amidites, methods of making these compounds and methods for detecting single nucleotide polymorphisms using the above OTNTPs. Methods for detecting differential gene expression using the OTNTPs and methods of separating specifically modified DNA or RNA using the OTNTPs are also provided.

Abstract: A synthesized reflector surface (12) for directing communication signals (27) in a communication system (10) that operates in a plurality of orbital slots and to a plurality of regions (28) within a first coverage area (30) is provided. The synthesized reflector surface (12) includes a plurality of contiguous profile surfaces (40) that form the reflector surface (12). Each of the plurality of contiguous profile surfaces (40) alters the phase-of the communication signals (27) to provide a first gain for a first satellite orbit location (32) and a second gain for a second satellite orbit location (34). The plurality of contiguous profile surfaces (40) directs the signals from the location (32) in a first orientation to the first coverage area (30) or from the location (34) in a second orientation to the first coverage area (30). A method is provided for synthesizing the reflector surface (12).

Abstract: A synthesized reflector surface (12) for directing communication signals (27) in a communication system (10) that operates in a plurality of orbital slots and to a plurality of regions (28) within a first coverage area (30) is provided. The synthesized reflector surface (12) includes a plurality of contiguous profile surfaces (40) that form the reflector surface (12). Each of the plurality of contiguous profile surfaces (40) alters the phase of the communication signals (27) to provide a first gain for a first satellite orbit location (32) and a second gain for a second satellite orbit location (34). The plurality of contiguous profile surfaces (40) directs the signals from the location (32) in a first orientation to the first coverage area (30) or from the location (34) in a second orientation to the first coverage area (30). A method is provided for synthesizing the reflector surface (12).