Abstract: For perfusion, blood vessels protruding from a removed organ or tissue and cannulas for circulating perfusate are tightened and bound at positions away from the cut ends of the blood vessels toward a proximal side viewed from the organ. The cut ends of the aforementioned blood vessels that are anastomosed to blood vessels of a living body are spaced from the binding positions at which the cannulas are secured. The direction to the blood vessels of the living body can be conducted while moving the cannulas in various postures relative to the blood vessels protruding from the organ or tissue. This enables performing an anastomosis operation while maintaining the perfusion state. In this way, organs and tissues that include at least one artery route and at least one vein route can be anastomosed to blood vessels of a living body while maintaining the perfusion state.

Abstract: A perfusion device and a perfusion method can allocate two routes of perfusate inflow pathways and two routes of perfusate outflow pathways for a liver graft. The perfusate inflow pathways have perfusate inflow cannulas connected respectively to the portal vein and the hepatic artery. The perfusate outflow pathways have perfusate outflow cannulas connected respectively to the suprahepatic inferior vena cava and the infrahepatic inferior vena cava. Perfusate is allowed to enter the liver from the perfusate inflow cannulas, and the perfusate in the liver is allowed to drain off from the perfusate outflow cannulas. This significantly shortens the time length of the liver graft being in an ischemic condition when the liver is removed or transplanted. That is, the onset of disorder in the liver graft can be suppressed. This increases the success rate of liver transplantation. Thus, deterioration of organ grafts during surgery is prevented in organ transplantation surgery that requires long hours.