Abstract: An object of the invention is to provide a water splitting device having a low electrolysis voltage and excellent gas separation performance. The water splitting device of the invention is a water splitting device that generates gases from the positive electrode and the negative electrode, the water splitting device including: a bath to be filled with an electrolytic aqueous solution; the positive electrode and the negative electrode disposed in the bath; and a polymer membrane that is ion-permeable and is disposed between the positive electrode and the negative electrode in order to separate the electrolytic aqueous solution filling the bath into the positive electrode side and the negative electrode side, wherein the positive electrode and the negative electrode are installed at a predetermined distance from the polymer membrane, and the moisture content of the polymer membrane is 40% or more.

Abstract: An object of the present invention is to provide a biosensor comprising a hydrogel capable of immobilizing a physiologically active substance thereon, which can be produced conveniently by use of a safe material, and a method for producing the same. The present invention provides a method for producing a biosensor, which comprises bringing a polymer containing an activated carboxyl group into contact with a substrate surface coated with an organic layer having an amino group to thereby bind the polymer to the organic layer.

Abstract: An object of the present invention is to provide a biosensor comprising a hydrogel capable of immobilizing a physiologically active substance thereon, which can be produced conveniently by use of a safe material, and a method for producing the same. The present invention provides a method for producing a biosensor, which comprises bringing a polymer containing an activated carboxyl group into contact with a substrate surface coated with an organic layer having an amino group to thereby bind the polymer to the organic layer.

Abstract: A water purification method comprising adding a purification agent to water having a contaminant concentration of 1 ?g/L to 10 g/L, the purification agent containing an adsorbent having an average particle size of 100 nm to 500 ?m, an iron-based flocculant, and an alkaline substance; causing the adsorbent to adsorb at least a part of the contaminants in water; settling the adsorbent with the adsorbed contaminants by the iron-based flocculant; and removing the sediment from water, wherein the purification agent is added in an amount of 0.01 g to 20 g per liter of water, can purify contaminated water conveniently and efficiently.

Abstract: One object of the present invention is to provide a biosensor and a production method therefor, by which hydrogel that enables immobilization of a physiologically active substance can be conveniently produced using safe raw materials. The present invention provides a biosensor which comprises a substrate having a metal layer on its surface, wherein a hydrophilic polymer having a reactive functional group capable of reacting with a hydroxyl group or an amino group of a physiologically active substance is bound to the metal layer directly or indirectly via an intermediate layer.

Abstract: Thus, the present invention provides a method for producing a biosensor comprising a substrate coated with a hydrophilic polymer, which comprises allowing a compound generating a reactive group as a result of external stimulus to bind to the surface of a substrate, and then giving external stimulus in a state where a hydrophilic polymer capable of forming a covalent bond with said reactive group is allowed to come into contact with the substrate, so that said hydrophilic polymer is allowed to bind to the substrate surface via a covalent bond with said reactive group.

Abstract: An immobilization method for immobilizing a physiologically active substance on a solid phase carrier, the method including: bringing the solid phase carrier into contact with an acid anhydride functional group-containing silane coupling agent represented by the following Formula (I); and carrying out a process of binding of the physiologically active substance to the acid anhydride functional group while maintaining the solid phase carrier after the contact at a temperature within the range of 0° C. to 60° C.; a physiologically active substance-immobilized carrier, and a carrier for immobilization are provided. Further, a carrier including a porous material treated with an acid anhydride functional group-containing silane coupling agent represented by the following Formula (I), a blocking agent that is immobilized to the porous material; and a method for producing it is provided.

Abstract: An immobilization method for immobilizing a physiologically active substance on a solid phase carrier, the method including: bringing the solid phase carrier into contact with an acid anhydride functional group-containing silane coupling agent represented by the following Formula (I); and carrying out a process of binding of the physiologically active substance to the acid anhydride functional group while maintaining the solid phase carrier after the contact at a temperature within the range of 0° C. to 60° C.; a physiologically active substance-immobilized carrier, and a carrier for immobilization are provided. Further, a carrier including a porous material treated with an acid anhydride functional group-containing silane coupling agent represented by the following Formula (I), a blocking agent that is immobilized to the porous material; and a method for producing it is provided.

Abstract: An object of the present invention is to provide a biochemical instrument having a surface that can simultaneously inhibit adsorption of a biopolymer and that of a hydrophobic low-molecular-weight compound. The present invention provides a biochemical instrument comprising a region that inhibits adsorption of a hydrophobic low-molecular-weight compound and a region that inhibits adsorption of a biopolymer on the surface of a water-insoluble material.

Abstract: A method of manufacturing a polymer film having a microphase-separated morphology includes a film-forming step in which a solution containing a block copolymer having two or more types of polymer chains which are mutually incompatible and are bonded to each other, a first solvent and a second solvent having a higher boiling point by at least 15° C. than the first solvent is applied onto a substrate and the first solvent is removed to form a film; a heating step in which the film is heated at a temperature which is higher than the boiling point of the first solvent but is lower than the boiling point of the second solvent to make the film formed have a microphase-separated morphology; and a solvent removal step in which the second solvent is removed from the film having the microphase-separated morphology to form the polymer film having the microphase-separated morphology.

Abstract: An object of the present invention is to provide a biosensor and a method for immobilizing a physiologically active substance, by which preconcentration effects can be obtained at a pH that is equivalent to or higher than the isoelectric point of the physiologically active substance and the physiologically active substance can be covalently bound to the surface. The present invention provides a biosensor comprising a solid substrate to which a polymer having a primary or secondary amino group is bound, by which a physiologically active substance can be chemically immobilized following preconcentration of the substance at a pH that is equivalent to or higher than the isoelectric point of the substance.

Abstract: A free standing film having excellent mechanical strength and flexibility as well as sufficient area is provided. The organic-inorganic hybrid free standing film includes an organic polymer having a constitutional repeating unit derived from a monomer represented by general formula (1) and a hydrolytic condensate of a metal alkoxide.

Abstract: The present invention provides a biosensor, wherein non-specific adsorption on a surface that does not retain a physiologically active substance (reference unit) is suppressed. The biosensor of the invention includes a substrate having at least two types of surfaces on a single plane, wherein at least one of the surfaces does not retain a physiologically active substance, and wherein at least two types of hydrophilic compounds with different molecular weights are bound to the surface that does not retain a physiologically active substance.

Abstract: A method for purifying bioactive substances includes the steps of: causing a bioactive substance having histidine units to contact media, each constituted by a substrate, ligands which are physically attached to the surface of the substrate, and Cu(II) or Fe(II) metal ions which are covalently bonded to the ligands; causing the bioactive substance to covalently bond with the metal ions via the histidine units; and washing the media with an amount of 1 nmol/L to 10 mmol/L imidazole derivative solution 60 times the volume of the media or greater. In the case that the metal ions are Cu(II), the bioactive substance which has covalently bonded with the Cu(II) via the histidine units are recovered by one of a 10 mmol/L to 1 mol/L imidazole derivative solution and a 0.5 mmol/L to 5 mol/L EDTA solution.

Abstract: A method of manufacturing a polymer film having a microphase-separated morphology includes a film-forming step in which a solution containing a block copolymer having two or more types of polymer chains which are mutually incompatible and are bonded to each other, a first solvent and a second solvent having a higher boiling point by at least 15° C. than the first solvent is applied onto a substrate and the first solvent is removed to form a film; a heating step in which the film is heated at a temperature which is higher than the boiling point of the first solvent but is lower than the boiling point of the second solvent to make the film formed have a microphase-separated morphology; and a solvent removal step in which the second solvent is removed from the film having the microphase-separated morphology to form the polymer film having the microphase-separated morphology.

Abstract: An immobilization method for immobilizing a physiologically active substance on a solid phase carrier, the method including: bringing the solid phase carrier into contact with an acid anhydride functional group-containing silane coupling agent represented by the following Formula (I); and carrying out a process of binding of the physiologically active substance to the acid anhydride functional group while maintaining the solid phase carrier after the contact at a temperature within the range of 0° C. to 60° C.; a physiologically active substance-immobilized carrier, and a carrier for immobilization are provided. Further, a carrier including a porous material treated with an acid anhydride functional group-containing silane coupling agent represented by the following Formula (I), a blocking agent that is immobilized to the porous material; and a method for producing it is provided.

Abstract: An object of the present invention is to provide a biosensor and a method for immobilizing a physiologically active substance, by which preconcentration effects can be obtained at a pH that is equivalent to or higher than the isoelectric point of the physiologically active substance and the physiologically active substance can be covalently bound to the surface. The present invention provides a biosensor comprising a solid substrate to which a polymer having a primary or secondary amino group is bound, by which a physiologically active substance can be chemically immobilized following preconcentration of the substance at a pH that is equivalent to or higher than the isoelectric point of the substance.

Abstract: One object of the present invention is to provide a biosensor and a production method therefor, by which hydrogel that enables immobilization of a physiologically active substance can be conveniently produced using safe raw materials. The present invention provides a biosensor which comprises a substrate having a metal layer on its surface, wherein a hydrophilic polymer having a reactive functional group capable of reacting with a hydroxyl group or an amino group of a physiologically active substance is bound to the metal layer directly or indirectly via an intermediate layer.

Abstract: A structure having on a flexible substrate an organic-inorganic composite layer which contains a polymer and a metal as its main components and in which one of the components forms microdomains oriented perpendicularly to the substrate by using a microphase-separated morphology formed from a block copolymer, and a manufacturing method capable of manufacturing the structure at a low cost and over a large surface area are provided. The structure includes in order on the flexible substrate, a conductive layer; an adsorbing compound layer, and the organic-inorganic composite layer having the microphase-separated morphology which includes a polymer phase and a metal phase and in which one of the phases makes up cylindrical or lamellar microdomains oriented perpendicularly to the flexible substrate.

Abstract: It is an object of the present invention to provide a technique of conserving a functional group used for binding a physiologically active substance in the form of an inactive ester, and converting it to an activated ester by simple operations immediately before formation of an amide bond. The present invention provides a biosensor which comprises a substrate that has been modified with a phenyl ester group having a substituent whose electron-withdrawing ability is increased as a result of a chemical reaction.