Abstract: A structure includes a flexible substrate and, in order thereon, a metal layer, a metal adsorbing compound layer, and a block copolymer layer which has a microphase-separated morphology and is formed of a block copolymer obtained by bonding two or more mutually incompatible polymer chains. The microphase-separated morphology has one phase which is lamellar or cylindrical, and is oriented perpendicularly to the substrate.

Abstract: An object of the present invention is to provide a biochemical instrument having a surface that can simultaneously inhibit adsorption of a biopolymer and that of a hydrophobic low-molecular-weight compound. The present invention provides a biochemical instrument comprising a region that inhibits adsorption of a hydrophobic low-molecular-weight compound and a region that inhibits adsorption of a biopolymer on the surface of a water-insoluble material.

Abstract: A free standing film having excellent mechanical strength and flexibility as well as sufficient area is provided. The organic-inorganic hybrid free standing film includes an organic polymer having a constitutional repeating unit derived from a monomer represented by general formula (1) and a hydrolytic condensate of a metal alkoxide.

Abstract: A structure having a block copolymer layer with a microphase-separated morphology in which a cylindrical or lamellar phase is oriented perpendicularly to a flexible substrate such as a polymer substrate is provided. The structure includes a flexible substrate and, in order thereon, a metal oxide layer, a layer formed with a silane coupling agent, and a layer which has a microphase-separated morphology and is formed of a block copolymer obtained by bonding two or more mutually incompatible polymer chains. The microphase-separated morphology has one phase which is lamellar or cylindrical, and oriented perpendicularly to the substrate.

Abstract: A porous film which is formed using a block copolymer composed of a water-soluble polymer and a water-insoluble polymer, has nanometer-size pores, and in which a desired functional polymer is present on the pore inner walls is provided. The porous film includes a microphase-separated morphology including a continuous phase which is composed primarily of a water-insoluble polymer A, and a plurality of cylindrical microdomains which are composed primarily of a water-soluble polymer B incompatible with the water-insoluble polymer A, distributed within the continuous phase and oriented perpendicular to a surface of the film. The cylindrical microdomains contain therein pores having a cylindrical shape and an average diameter of between 1 and 200 nm.

Abstract: It is an object of the present invention to provide a biosensor on which a large amount of protein can be immobilized and nonspecific adsorption is less likely to occur with the use of a SAM compound having high water solubility and good performance in terms of supply. The present invention provides a biosensor comprising a substrate on which a hydrophilic polymer having a reactive group capable of binding to a physiologically active substance is immobilized via a compound represented by the following formula (1), said compound at a concentration of 1 mM being dissolved in water at 25° C.: X-L-Y??(1) wherein X represents a group capable of binding to metal, L represents a linking group, and Y represents a functional group to which the hydrophilic polymer is bound.

Abstract: A method for purifying bioactive substances includes the steps of: causing a bioactive substance having histidine units to contact media, each constituted by a substrate, ligands which are physically attached to the surface of the substrate, and Cu(II) or Fe(II) metal ions which are covalently bonded to the ligands; causing the bioactive substance to covalently bond with the metal ions via the histidine units; and washing the media with an amount of 1 nmol/L to 10 mmol/L imidazole derivative solution 60 times the volume of the media or greater. In the case that the metal ions are Cu(II), the bioactive substance which has covalently bonded with the Cu(II) via the histidine units are recovered by one of a 10 mmol/L to 1 mol/L imidazole derivative solution and a 0.5 mmol/L to 5 mol/L EDTA solution.

Abstract: A biosensor chip includes a substrate, a polymer having an anionic functional group and being arranged on a surface of the substrate, a polyamino group which is directly or indirectly bound to the anionic functional group at a surface of the polymer, and a long-chain alkyl-based group which is directly or indirectly bound to the anionic functional group at the surface of the polymer.

Abstract: It is an object of the present invention to provide a solid substrate, on which a physiologically active substance is inclusively immobilized, and a production method thereof. The present invention provides a solid substrate, which has a physiologically active substance in a crosslinked hydrogel composed of a polysaccharide that was covalently bound to the surface of the solid substrate.

Abstract: It is an object of the present invention to provide a biosensor substrate that prevents non-specific adsorption and is used for immobilizing a physiologically active substance. The present invention provides a biosensor substrate which comprises an inorganic oxide film and a hydrophilic polymer having a physiologically active substance-immobilizing group on the substrate, wherein the hydrophilic polymer is bound to the inorganic oxide film through a low molecular compound.

Abstract: An object of the present invention is to provide a method for production of a physiologically active substance-immobilized substrate, which can prevent the inactivation of a physiologically active substance and/or can immobilize a physiologically active substance at a high concentration onto a substrate surface without use of electrostatic attraction. The present invention provides a method for production of a physiologically active substance-immobilized substrate, which comprises steps of: applying, to a metal substrate having a layer for immobilizing a physiologically active substance, a solution containing a physiologically active substance capable of forming a covalent bond with a molecule constituting the layer for immobilizing a physiologically active substance; and then drying the solution.

Abstract: In a gas barrier laminate film comprising a base material film containing an inorganic compound and at least one set of inorganic layer and organic layer formed on the base material film, the base material film is formed with a resin having a glass transition temperature of 25° C. or higher. A gas barrier laminate film that has superior durability, heat resistance and gas barrier performance, shows a small difference in coefficient of linear expansion relative to a contiguous layer and can maintain superior gas barrier property even if it is bent is provided.

Abstract: It is an object of the present invention to provide a biochemical instrument wherein an adhesive layer that has hydrophilic properties, heat resistance, and satisfactory adhesiveness is provided between a plastic material surface and a hydrophilic polymer layer. The present invention provides a biochemical instrument comprising a plastic material that is coated on its surface with a hydrophilic polymer, which comprises a metal oxide thin film between the plastic material and the hydrophilic polymer layer, wherein the film stress of said metal oxide thin film is less than 5.4×1021 dyn/cm2.

Abstract: A tetraalkylphosphonium salt having at least one branched alkyl chain containing 9 to 100 carbon atoms in total, an organically modified layered silicate containing the tetraalkylphosphonium salt having at least one branched alkyl chain containing 9 to 100 carbon atoms in total between layers of a layered silicate and a composition comprising the organically modified layered silicate and an organic solvent or a thermoplastic resin. There are provided a tetraalkylphosphonium salt for a layered silicate having thermal stability and dispersibility superior to those of conventional organophilized layered silicates, an organically modified layered silicate containing the tetraalkylphosphonium salt and a composition thereof.

Abstract: A polymer composition includes a polymer having a glass transition temperature of 120° C. to 400° C. as a simple substance of the polymer and an organic modified layered silicate having a decomposition starting temperature of 190° C. to 350° C. and contained in the polymer. (1) A film including the polymer composition, (2) a gas barrier film including the film and an organic/inorganic hybrid layer obtained by the sol-gel method and provided on the film, (3) a substrate comprising the film or the gas barrier film, and (4) an image display device comprising at least an anode, a light-emitting organic thin film layer and a cathode on the film or the gas barrier film are also disclosed. A polymer composition having both superior heat resistance and superior gas baffler properties, a film comprising the polymer composition, and a gas baffler film having the film, as well as a substrate and image display device exhibiting superior precision and durability are provided.

Abstract: It is an object of the present invention to provide a biosensor on which a large amount of protein can be immobilized and nonspecific adsorption is less likely to occur with the use of a SAM compound having high water solubility and good performance in terms of supply. The present invention provides a biosensor comprising a substrate on which a hydrophilic polymer having a reactive group capable of binding to a physiologically active substance is immobilized via a compound represented by the following formula (1), said compound at a concentration of 1 mM being dissolved in water at 25° C.: X-L-Y??(1) wherein X represents a group capable of binding to metal, L represents a linking group, and Y represents a functional group to which the hydrophilic polymer is bound.

Abstract: Thus, the present invention provides a method for producing a biosensor comprising a substrate coated with a hydrophilic polymer, which comprises allowing a compound generating a reactive group as a result of external stimulus to bind to the surface of a substrate, and then giving external stimulus in a state where a hydrophilic polymer capable of forming a covalent bond with said reactive group is allowed to come into contact with the substrate, so that said hydrophilic polymer is allowed to bind to the substrate surface via a covalent bond with said reactive group.

Abstract: An object of the present invention is to provide a biosensor comprising a hydrogel capable of immobilizing a physiologically active substance thereon, which can be produced conveniently by use of a safe material, and a method for producing the same. The present invention provides a method for producing a biosensor, which comprises bringing a polymer containing an activated carboxyl group into contact with a substrate surface coated with an organic layer having an amino group to thereby bind the polymer to the organic layer.

Abstract: An object of the present invention is to provide a biosensor and a method for immobilizing a physiologically active substance, by which preconcentration effects can be obtained at a pH that is equivalent to or higher than the isoelectric point of the physiologically active substance and the physiologically active substance can be covalently bound to the surface. The present invention provides a biosensor comprising a solid substrate to which a polymer having a primary or secondary amino group is bound, by which a physiologically active substance can be chemically immobilized following preconcentration of the substance at a pH that is equivalent to or higher than the isoelectric point of the substance.

Abstract: An object of the present invention is to provide a biosensor having high capability for suppressing nonspecific protein adsorption and high capability for extracting a target protein. The present invention provides a biosensor which comprises a flow channel that is formed on a substrate and is composed of a detection plane for detecting interaction between a physiologically active substance and a test substance and a non-detection plane where said interaction is not detected, wherein the substrate is a metal surface or a metal film and the surfaces of the detection plane and the non-detection plane are modified with a self-assembled monolayer (SAM) having a hydroxy group and a functional group for binding with a physiologically active substance.