Abstract: A method for producing the cell culture vessel having the culture surface of cells includes: using a primary mold including a projecting portion to form an uneven pattern structure on a surface of a vessel base material and a secondary mold including a projecting portion to flatten a part of the surface of the vessel base material; pressing the primary mold against the vessel base material to form the uneven pattern structure on the vessel base material; pressing the secondary mold against a thin portion formed on the vessel base material to modify the thin portion to a flattened portion; and configuring the surface of the vessel base material including the uneven pattern structure formed with at least a part of the modified flattened portion as the culture surface to form the cell culture vessel.

Abstract: A culture vessel includes at least a first port used for medium supply and a second port used for medium discharge, and A medium supplying vessel serves as a mixing vessel for adjusting a concentration of A new medium to be supplied to the culture vessel. A cell culture method includes: transferring a portion of a used medium in the culture vessel from the culture vessel to the mixing vessel; creating a concentration-adjusted medium by mixing the new medium and the portion of the used medium in the mixing vessel; and filling the culture vessel with the concentration-adjusted medium by discharging a remaining used medium from the culture vessel while the concentration-adjusted medium is transferred from the mixing vessel to the culture vessel.

Abstract: Provided is a cell culture system capable of detecting a proliferative property of cells in a culture vessel at a desired timing during a culture when the cells are statically cultured in the culture vessel formed of a gas permeable member. A cell culture system is a system for statically culturing cells with a culture vessel at least partially formed of a gas permeable member. The cell culture system includes an oxygen concentration sensor that measures an oxygen concentration in a vicinity of a culture surface inside the culture vessel, and a proliferation detection unit that detects a proliferative property of the cells based on an oxygen permeability of the gas permeable member, a measurement value of the oxygen concentration sensor, an oxygen concentration around the culture vessel, and an oxygen consumption per cell of the cells.

Abstract: A culture container for culturing lymphocytes includes an immobilized surface and a non-immobilized surface, wherein the culture container is formed of a gas permeable film, the immobilized surface and the non-immobilized surface are container inner surfaces facing each other, and anti-CD3 antibodies are immobilized in the immobilized surface at a concentration of 10 to 300 ng/cm2.

Abstract: Provided are a multilayer film having excellent gas permeability and excellent handling properties and hence is suited for forming a cell culture container, and a cell culture container formed by using the same. A multilayer film used for forming a cell culture container, comprising: a base material composed of a polyethylene-based resin having a density of 0.87 g/cm3 to 0.90 g/cm3; and an inner layer composed of a polyethylene-based resin having a density of 0.896 g/cm3 to 0.93 g/cm3 and forming a cell culture icy surface. A cell culture container is formed by using this multilayer film.

Abstract: Provided is a cell culture vessel for culturing cells with a high density having excellent gas permeability and strength. A bag-shaped cell culture vessel of a closed system includes at least one port and mutually opposed planar substrates. At least one of the planar substrates is formed of a gas permeable film, the at least one gas permeable film has an outer surface provided with a protruding portion having a shape different from an inner surface shape of the gas permeable film, a culture space for culturing cells is provided in the inner surface side of the gas permeable film, and when the gas permeable film is brought in contact with a planar surface, a space enabling ventilation is formed between the gas permeable film and the planar surface by the protruding portion.

Abstract: Provided is a culture vessel for adherent cells that has a large culture portion surface area and ensures easily dissociating and recovering cells without consuming a medium or a dissociation solution more than necessary. The bag-shaped adherent cell culture vessel made of flexible packaging material includes a vessel body portion having a first vessel wall and a second vessel wall and one or more injecting/ejecting ports. The vessel body portion has an intermediate culture wall between the first vessel wall and the second vessel wall. A culture chamber is disposed in each of between the first vessel wall and the intermediate culture wall and between the second vessel wall and the intermediate culture wall, and a flow passage communicating between the respective culture chambers is disposed.

Abstract: [Object] To provide a vessel suitable for efficient culture and differentiation induction or the like of cells in the same vessel while reducing any contamination risk, and also to provide a cell culture vessel capable of ensuring provision of a flat culture surface over a wide range. [Solving Means] A vessel manufacturing method includes placing a bag-shaped, film-based vessel on a placement stage with concave portions formed on the stage, introducing fluid into the vessel placed on the stage, and pressing the vessel which is placed on the stage, by a pressing member while heating at least one of the stage and the pressing member. Meanwhile, a cell culture vessel includes a first vessel wall as a bottom wall and a second vessel wall. The first vessel wall is formed of a flat film having gas permeability. The second vessel wall is disposed in contact with a peripheral edge portion of the first vessel wall, and has a bulge shape protruding relative to the first vessel wall.

Abstract: A culture container made of a flexible packaging material where the culture container includes a first side surface portion and a second side surface portion that are mutually opposed. The culture container also includes at least an inner surface side of the first side surface portion which has a surface treatment such that, when a contact angle hysteresis is defined as an advance contact angle—a retreat contact angle at a time when water slips, a contact angle hysteresis of a surface on the inner surface side of the first side surface portion becomes larger than a contact angle hysteresis of a surface on an inner surface side of the second side surface portion. The culture container further includes a membrane made of a cell adhesion inhibitor that is formed on the inner surface side of the first side surface portion.

Abstract: A cell culture container includes a container main body comprising a gas-permeable plastic film, and an injection/discharge port. A peripheral portion of the container main body is sealed, and the container main body has a bulging shape on a top surface side of the container main body. A plurality of concave portions serving as cell culture parts are provided on a bottom surface of the container main body.

Abstract: A culture container for culturing lymphocytes includes an immobilized surface and a non-immobilized surface, wherein the culture container is formed of a gas permeable film, the immobilized surface and the non-immobilized surface are container inner surfaces facing each other, and anti-CD3 antibodies are immobilized in the immobilized surface at a concentration of 10 to 300 ng/cm2.

Abstract: A culture container for culturing lymphocytes includes an immobilized surface and a non-immobilized surface, wherein the culture container is formed of a gas permeable film, the immobilized surface and the non-immobilized surface are container inner surfaces facing each other, and anti-CD3 antibodies are immobilized in the immobilized surface at a concentration of 10 to 300 ng/cm2.

Abstract: A culture container for culturing lymphocytes includes an immobilized surface and a non-immobilized surface, wherein the culture container is formed of a gas permeable film, the immobilized surface and the non-immobilized surface are container inner surfaces facing each other, and anti-CD3 antibodies are immobilized in the immobilized surface at a concentration of 10 to 300 ng/cm2.

Abstract: In the production of a culture vessel in which a low cell-adhesive polymer as a coating agent is applied to a culture surface having a plurality of concave parts formed thereon, a use amount of the coating agent can be reduced. The low cell-adhesive polymer as the coating agent is applied to a surface of a culture base material in advance, and subsequently the culture base material is subjected to processing to form the culture vessel having the surface of the culture base material coated with the coating agent as the culture surface.

Abstract: Provided is a culture container base material made of a polyolefin material, capable of readily forming a culture container for culturing adherent cells. The culture container base material is for culturing the adherent cells and made of the polyolefin material. At least a part of a surface of the base material is subjected to a surface treatment, where the surface of the base material is a culture surface of the culture container, and the surface subjected to the surface treatment has a static water contact angle of greater than 80° and a receding contact angle of less than 53°.

Abstract: A culture container for activating lymphocytes includes immobilized anti-CD3 antibodies and an anti-CD3 antibody solution including anti-CD3 antibodies, wherein the culture container is formed in a bag-like shape and is formed of a soft-packaging material, the immobilized anti-CD3 antibodies are immobilized at a density of 10 to 300 ng/cm2 on one surface of opposing inner surfaces within the container, and the anti-CD3 antibody solution is enclosed in the container in an amount of 0.25 to 400 ng of the anti-CD3 antibodies in 0.1 to 800 ?l of the solution per 1 cm2 of a culture surface formed of the one surface.

Abstract: A culture container made of a flexible packaging material where the culture container includes a first side surface portion and a second side surface portion that are mutually opposed. The culture container also includes at least an inner surface side of the first side surface portion which has a surface treatment such that, when a contact angle hysteresis is defined as an advance contact angle—a retreat contact angle at a time when water slips, a contact angle hysteresis of a surface on the inner surface side of the first side surface portion becomes larger than a contact angle hysteresis of a surface on an inner surface side of the second side surface portion. The culture container further includes a membrane made of a cell adhesion inhibitor that is formed on the inner surface side of the first side surface portion.

Abstract: A cell culture method and a cell culture system, wherein: at least a part of a culture surface of a culture container is formed of a base material, the base material having a contact angle of not more than 84°, being selected from polyethylene, polypropylene, polymethylpentene, cyclic olefin polymer, cyclic olefin copolymer, polyvinyl chloride, polyurethane, methyl polymethacrylate, polyester, polyamide, ionomer, ethylene-vinyl acetate copolymer, ethylene-vinyl alcohol copolymer, ethylene-acrylic acid copolymer, ethylene-methyl acrylate copolymer, ethylene-methacrylic acid copolymer, ethylene-methyl methacrylate copolymer and the like, and having been irradiated or hydrophilized; a medium containing laminin or a fragment thereof as a cell adhesion factor and cells are accommodated in the culture container; and adherent cells are thus cultured so that the adherent cells can be preferably cultured without coating the culture container with laminin.

Abstract: Provided is a mass culture method for cells, which uses a culture vessel having flexibility, can form cell aggregates as needed, and is reduced in contamination risk. A pressure is applied onto a culture vessel, which has flexibility, is filled with cells and a culture fluid, and is placed on a placement surface having one or more depressions. With recessed and projected portions, which conform to the depressions, being formed on and in an outer surface of the culture fluid vessel under the application of the pressure, the cells in the culture vessel are cultured.

Abstract: A vessel manufacturing method includes placing a bag-shaped, film-based vessel on a placement stage with concave portions formed on the stage, introducing fluid into the vessel placed on the stage, and pressing the vessel which is placed on the stage, by a pressing member while heating at least one of the stage and the pressing member. Meanwhile, a cell culture vessel includes a first vessel wall as a bottom wall and a second vessel wall. The first vessel wall is formed of a flat film having gas permeability. The second vessel wall is disposed in contact with a peripheral edge portion of the first vessel wall, and has a bulge shape protruding relative to the first vessel wall. The first vessel wall is flat at its section other than a region where the first vessel wall is in contact with at least a charge/discharge port.