Abstract: Provided is a container for cell culture, provided with a container main body and injection/discharge ports, in which an inside of the container main body is divided into a plurality of cisterns 1st and 2nd by a filter member. Thus, various operations performed upon the cell culture can be simply and efficiently performed.

Abstract: Provided are a multilayer film having excellent gas permeability and excellent handling properties and hence is suited for forming a cell culture container, and a cell culture container formed by using the same. A multilayer film used for forming a cell culture container, comprising: a base material composed of a polyethylene-based resin having a density of 0.87 g/cm3 to 0.90 g/cm3; and an inner layer composed of a polyethylene-based resin having a density of 0.896 g/cm3 to 0.93 g/cm3 and forming a cell culture icy surface. A cell culture container is formed by using this multilayer film.

Abstract: A cell culture container includes a container main body comprising a gas-permeable plastic film, and an injection/discharge port. A peripheral portion of the container main body is sealed, and the container main body has a bulging shape on a top surface side of the container main body. A plurality of concave portions serving as cell culture parts are provided on a bottom surface of the container main body.

Abstract: A culture bag for culturing adherent cells is provided without requiring a highly clean production environment and a complex production step such as a masking step. An adherent cell culture vessel that is formed of a polyolefin is configured so that part or the entirety of the inner surface of the culture vessel has a static water contact angle of 95° or more, and has an advancing contact angle and a receding contact angle that satisfy the inequality “advancing contact angle?receding contact angle>25°” when water runs down along the inner surface.

Abstract: A cell culture kit for cultivating cells in a closed system includes at least one or more of each of: a culture container for cultivating cells; a culture medium storage container for storing a culture medium or the like; a cell injection container for injecting cells; and a cell collecting container for collecting a suspension of cells after cultivation; wherein the culture medium storage container is also used as a waste liquid container for collecting a culture medium after cultivation, and the culture container, the culture medium storage container, the cell injection container and the cell collecting container are linked to one another through a conduit.

Abstract: A culture container for activating lymphocytes includes immobilized anti-CD3 antibodies and an anti-CD3 antibody solution including anti-CD3 antibodies, wherein the culture container is formed in a bag-like shape and is formed of a soft-packaging material, the immobilized anti-CD3 antibodies are immobilized at a density of 10 to 300 ng/cm2 on one surface of opposing inner surfaces within the container, and the anti-CD3 antibody solution is enclosed in the container in an amount of 0.25 to 400 ng of the anti-CD3 antibodies in 0.1 to 800 ?l of the solution per 1 cm2 of a culture surface formed of the one surface.

Abstract: Adhesive culture cells cultured by using a culture container made of a flexible film can selectively be released from the culture container without causing damage to the cells. A cell release method for releasing, from a culture container, adhesive culture cells cultured adhering to the culture container made of a flexible film includes bringing a surface of the film of the culture container into contact with the adhesive culture cells, applying a pressure to the adhesive culture cells, and releasing the adhesive culture cells from the culture container, wherein the surface of the film faces a surface of the film of the culture container to which the adhesive culture cells adhere.

Abstract: To provide a culture container that can transfer cells cultured under a plurality of different culture conditions by a simple work. Provided is a multi-chamber culture container obtained by processing a material having gas permeability, wherein two or more culture chambers for culturing cells are formed, each of the culture chambers are intercommunicated with at least of one other culture chamber and at least one external connection part for intercommunicating with the outside of the multi-chamber culture container is provided.

Abstract: A culture container for culturing lymphocytes includes an immobilized surface and a non-immobilized surface, wherein the culture container is formed of a gas permeable film, the immobilized surface and the non-immobilized surface are container inner surfaces facing each other, and anti-CD3 antibodies are immobilized in the immobilized surface at a concentration of 10 to 300 ng/cm2.

Abstract: Adhesive culture cells cultured by using a culture container made of a flexible film can selectively be released from the culture container without causing damage to the cells. A cell release method for releasing, from a culture container, adhesive culture cells cultured adhering to the culture container made of a flexible film includes bringing a surface of the film of the culture container into contact with the adhesive culture cells, applying a pressure to the adhesive culture cells, and releasing the adhesive culture cells from the culture container, wherein the surface of the film faces a surface of the film of the culture container to which the adhesive culture cells adhere.

Abstract: A method for counting counted targets disposed in a liquid enclosed in a container, includes adjusting a thickness of at least a part of the container; setting at least a part of the adjusted part as a measurement target region; and counting a number of counted targets in the measurement target region.

Abstract: A cell culture kit for cultivating cells in a closed system that comprises at least one or more of each of: a culture container for cultivating cells; a culture medium storage container for storing a culture medium or the like; a cell injection container for injecting cells; and a cell collecting container for collecting a suspension of cells after cultivation; wherein the culture medium storage container is also used as a waste liquid container for collecting a culture medium after cultivation, and the culture container, the culture medium storage container, the cell injection container and the cell collecting container are linked to one another through a conduit. Also provided is a cell culture kit including at least one or more of a sampling container that is capable of sampling part of a suspension of cells during cultivation, wherein the sampling container is linked to the culture container through a conduit.

Abstract: A method for counting counted targets disposed in a liquid enclosed in a container, includes adjusting a thickness of at least a part of the container; setting at least a part of the adjusted part as a measurement target region; and counting a number of counted targets in the measurement target region.

Abstract: A culture bag for culturing adherent cells is provided without requiring a highly clean production environment and a complex production step such as a masking step. An adherent cell culture vessel that is formed of a polyolefin is configured so that part or the entirety of the inner surface of the culture vessel has a static water contact angle of 95° or more, and has an advancing contact angle and a receding contact angle that satisfy the inequality “advancing contact angle?receding contact angle>25°” when water runs down along the inner surface.

Abstract: Provided are a multilayer film having excellent gas permeability and excellent handling properties and hence is suited for forming a cell culture container, and a cell culture container formed by using the same. A multilayer film used for forming a cell culture container, comprising: a base material composed of a polyethylene-based resin having a density of 0.87 g/cm3 to 0.90 g/cm3; and an inner layer composed of a polyethylene-based resin having a density of 0.896 g/cm3 to 0.93 g/cm3 and forming a cell culture surface. A cell culture container is formed by using this multilayer film.

Abstract: Cell aggregate formation control and cell aggregate disintegration control are carried out with respect to cells in a culture container to increase cell proliferation efficiency. The number of the cells in the culture container is counted without disassembly of a culture system and irrespective of the density of the cells. An external force is applied to the culture container to carry out at least one of cell aggregate formation control and cell aggregate disintegration control with respect to cells in the culture container, thus culturing the cells. The external force is applied to the culture container by pressing an agitating member onto a top surface of the culture container placed in flat orientation to a predetermined pressing degree, and moving the agitating member in a horizontal direction at a predetermined speed, thus controlling at least one of cell aggregate formation and cell aggregate disintegration with respect to the cells in the culture container.