Abstract: Novel full-length cDNAs are provided.

Abstract: Novel full-length cDNAs are provided.

Abstract: Novel full-length cDNAs are provided.

Abstract: The culture supernatant of COS cells expressing the PSEC56 gene was found to have a neurite extension effect. The main substance of the activity contained in this culture supernatant was revealed to be NGF. It was shown that PSEC56 can be utilized as a neurotrophic factor expression-inducing agent, and that the NGF induction system can be utilized for drug development against diseases of the nervous system.

Abstract: A cDNA fragment participating in the maintenance of smooth muscle differentiation was isolated using a culture system of chicken gizzard smooth muscle cells, the differential display method and the subtracted hybridization method. Using the obtained cDNA sequence as a query, cDNA sequences of Helix Research Institute (Japanese Patent Application No. 2000-118776) were retrieved, and thus, a novel gene “C-NT2RP3001495” was obtained. The protein encoded by this gene has two WW domains that participate in protein interactions in the N-terminal domain. Evidence suggests that this protein binds to other proteins, and thus regulates the intracellular signal transduction, gene expression, and so on, thereby participating in the maintenance of the differentiation of smooth muscle cells. This protein and compounds regulating the expression thereof are markedly useful in developing drugs for various diseases associated with abnormality in the maintenance of smooth muscle cell differentiation.

Abstract: Selection of clones having the kinase and/or phosphatase-like structure from clones which had been isolated and the structures thereof had been determined in the Helix Research Institute (helix clones; Japanese Patent Application No. 2000-183767) was conducted. Twelve novel genes were provided by carrying out homology search for all the helix clones by using the amino acid sequences of known kinases and phosphatases as queries. The genes are expected to be involved in intracellular signal transduction. The physiological functions of the inventive genes can be tested by using reporter gene assay systems capable of detecting signal transduction. The proteins of the present invention are useful as target molecules in drug discovery and in the development of new pharmaceuticals.

Abstract: Selection of clones having the kinase and/or phosphatase-like structure from clones which had been isolated and the structures thereof had been determined in the Helix Research Institute (helix clones; Japanese Patent Application No. 2000-183767) was conducted. Two novel genes were provided by carrying out homology search for all the helix clones by using the amino acid sequences of known kinases and phosphatases as queries. The genes are expected to be involved in intracellular signal transduction. The physiological functions of the inventive genes can be tested by using reporter gene assay systems capable of detecting signal transduction. The proteins of the present invention are useful as target molecules in drug discovery and in the development of new pharmaceuticals.

Abstract: The object of the present invention is to provide an amino acid frame indication system, a method for amino acid frame indication and a recording medium, which can effectively extract a highly reliable amino acid sequence from a cDNA sequence, even in a case where there exists a frame shift error in the cDNA sequence.

Abstract: D-amino acid oxidase (DAO) originated from Fusarium solani M-0718, a DNA compound encoding the oxidase, expression vectors containing said DNA compound are provided. Esherichia coli transformants capable of producing recombinant DAO in high level are also provided.

Abstract: The present invention provides a process for producing 7-aminocephem compounds or salts thereof.

Abstract: D-amino acid oxidase (DAO) originated from Fusarium solani M-0718, a DNA compound encoding the oxidase, expression vectors containing said DNA compound are provided. Esherichia coli transformants capable of producing recombinant DAO in high level are also provided.

Abstract: A cephalosporin C acylase from Pseudomonas diminuta N-176 is characterized by its ability to catalyze the conversion of cephalosporin C, glutaryl 7-ACA, adipyl 7-ACA, succinyl 7-ACA, N-acetylcephalosporin, N-benzoylcephalosporin C and cephalothin into 7-aminocephalosporanic acid. The enzyme contains an .alpha.-subunit having a molecular weight of 26 kDA and a .beta.-subunit having a molecular weight of 58 kDa. A method for the recombinant production of the present cephalosporin C acylase in Escherichia coli is provided.

Abstract: A cephalosporin C acylase from Pseudomonas diminuta N-176 is disclosed as well as the recombinant production of this enzyme in E. coli. The enzyme is characterized by the ability to catalyze the conversion of cephalosporin C, gultaryl 7-ACA, apidyl 7-ACA, succinyl 7-ACA, N-acetylcephalosporin C, N-benzoylcephalosporin C, and cephalothin into 7-aminocephalosporanic acid and is composed of an .alpha.-subunit with a molecular weight of 26,000 daltons and a .beta.-subunit with a molecular weight of 58,000 daltons.

Abstract: The present invention relates to new hybrid plasmids useful as vectors in recombinant DNA technology, in which Acremonium chrysogenum or Saccharomyces cerevisiae is used as a host, and to microorganisms bearing the hybrid plasmids.partially digesting a chromosomal DNA of Acremonium chrysogenum ATCC 11550 with restriction enzyme Sau 3A, said autonomous replication sequence having a molecular size of about 1.39 Kbp, and having the restriction maps set forth in FIGS. 4-6.

Abstract: New hybrid plasmids which are useful as vectors in recombinant DNA in which Acremonium chrysogenum or Saccharomyces cerevisiae is used as a host, and microorganisms bearing the hybrid plasmids, are disclosed. The disclosure herein is based on the cloning of autonomous replication sequences (ARS) of A. chrysogenum ATCC 11550 and introduction of the ARS into a shuttle vector of E. coli and Saccharomyces cerevisiae and construction of new types of plasmids based on the ARS.