Abstract: Provided is an electrophoresis device for enhancing its analysis performance. In order to achieve the task, the electrophoresis device includes a capillary array constituted by a capillary, a capillary head for bundling an end of the capillary, an electrode holder for holding an electrode provided at the other end of the capillary and a detector provided for the capillary, a first heater for heating the capillary, and an irradiation detection unit for irradiating a light to the detector to detect a fluorescence generated from a fluorescence labelled sample in the capillary. The electrophoresis device further includes a second heater for heating the detector.

Abstract: The present invention aims to provide an electrophoresis apparatus which makes it possible to execute protein analysis with a high throughput. The electrophoresis apparatus according to the present invention is equipped with a capillary array which is configured by arraying a plurality of capillaries, a measurement light irradiation unit which irradiates with measurement light, a first lens array which includes a plurality of first lenses which are arrayed in correspondence with the plurality of capillaries, a second lens array which includes a plurality of second lenses which are arrayed in correspondence with the plurality of capillaries, and a light receiving unit which receives light which is incident upon the capillaries via the first lens array from the measurement light irradiation unit via the second lens array.

Abstract: In an instrument configured to spectroscopically divide fluorescences emitted from a plurality of capillaries and collectively measure the fluorescences using an image sensor, when the number of pixels of a binning region on the image sensor on which a predetermined wavelength-band component of each fluorescence is projected is denoted by Bm, the number of pixels of hardware binning is denoted by Bh, the number of pixels of software binning is denoted by Bs, Bm=Bh×Bs, the total noise measured in a case where Bm=Bh=Bs=1 is denoted by N, the readout noise is denoted by Nr, the dark-current noise is denoted by Nd, and the shot noise is denoted by Ns, Bm, Bh, Bs, N, Nr, Nd, and Ns satisfy a predetermined relationship, thereby realizing high sensitivity and high dynamic range in fluorescence measurement.

Abstract: A capillary electrophoresis device includes: a light source; a plurality of capillaries; a light detection unit; and a plurality of detection optical fibers each having one end face disposed in association with corresponding one of the capillaries and another end face connected to the light detection unit, wherein the light detection unit selectively detects light in a central portion of the detection optical fiber.

Abstract: Efficient simultaneous laser-irradiation-fluorescence detection is performed for a plurality of channels of a microchip and simple and highly sensitive parallel analysis of a plurality of samples is enabled. In a microchip 1 made of an optically transparent solid material m1 with a refractive index n1, a plurality of channels 2 filled with a material m2 with a refractive index n2 and a plurality of channels 3 filled with a material m3 with a refractive index n3 are alternately arranged in parallel on the same plane. Here, m1, m2 and m3 are selected such that a relation of n2<n1<n3 is satisfied. The material m2 is an optically transparent liquid suitable for targeted analysis. When a laser beam 4 is focused and irradiated perpendicularly to the individual channels 2 and 3 along the same plane, refraction of the laser beam 4 by the channels 2 and refraction of the laser beam 4 by the channels 3 are counterbalanced.

Abstract: A capillary array includes N analysis capillaries filled with a separation medium having a refractive index of n3 and used for electrophoretic analysis and N±1 lens capillaries filled with a medium having a refractive index of n4 and not used for the electrophoretic analysis, in which the N analysis capillaries and the N±1 lens capillaries are alternately arranged on the same array plane. When an outer diameter is defined as 2R, an inner diameter is defined as 2r, a refractive index of a medium of an outside is n1, and a refractive index of a material is defined as n2 for each of the N analysis capillaries and the N±1 lens capillaries, and when a refractive index n3 takes any value within the range of 1.33?n3?1.42, R, r, n1, n2, n3, and n4 satisfy a predetermined relationship, whereby the N analysis capillaries are efficiently simultaneously irradiated with the laser beam incident from a side of the array plane.

Abstract: An analysis system includes an analyzer configured to separate a sample including a plurality of components labeled with any of M kinds of fluorescent substances by chromatography and acquire first time-series data of fluorescence signals detected in N kinds (M>N) of wavelength bands in a state in which at least a part of the plurality of components is not completely separated; and a computer configured to compare the first time-series data with the second time-series data, and determine which kind of fluorescent substance of M kinds of fluorescent substances individually labels each of the plurality of components.

Abstract: When arraying laser-irradiation portions of a plurality of capillaries on the same array plane, simultaneous irradiation of the capillaries with a laser beam incident from the side is enabled by filling the capillaries with a separation medium having a low refractive index of n3<1.36 and by setting n1=1.00, n2=1.46, R/r<5.9, and ?Z?9 ?m, wherein a distance in a direction perpendicular to the array plane between two capillaries, which are farthest from each other in the perpendicular direction among the capillaries in the laser-irradiation portions, is denoted by 2×?Z, wherein an outer radius, an inner radius, a refractive index of an external medium, a refractive index of a material, and a refractive index of an internal medium of each of the capillaries in the laser-irradiation portions are denoted by R, r, n1, n2, and n3, respectively.

Abstract: When laser-irradiation portions of N capillaries with capillary numbers n=1, 2, . . . , and N are arranged on a same plane, a laser-irradiation intensity of each capillary is denoted by L(n), and an output intensity of each capillary by a computer when a light emitting substance having an equal concentration exists inside each capillary is denoted by H(n), an absolute value of an average value of a second derivative of H(n) becomes smaller than an absolute value of an average value of a second derivative of L(n) for any refractive index n3 of a separation medium in a range of 1.33?n3?1.41 by digital correction by the computer, which changes according to the refractive index n3 (see FIG. 11).

Abstract: An analysis system includes an analyzer configured to separate a sample including a plurality of components labeled with any of M kinds of fluorescent substances by chromatography and acquire first time-series data of fluorescence signals detected in N kinds (M>N) of wavelength bands in a state in which at least a part of the plurality of components is not completely separated; and a computer configured to compare the first time-series data with the second time-series data, and determine which kind of fluorescent substance of M kinds of fluorescent substances individually labels each of the plurality of components.

Abstract: The present invention aims to provide an electrophoresis apparatus which makes it possible to execute protein analysis with a high throughput. The electrophoresis apparatus according to the present invention is equipped with a capillary array which is configured by arraying a plurality of capillaries, a measurement light irradiation unit which irradiates with measurement light, a first lens array which includes a plurality of first lenses which are arrayed in correspondence with the plurality of capillaries, a second lens array which includes a plurality of second lenses which are arrayed in correspondence with the plurality of capillaries, and a light receiving unit which receives light which is incident upon the capillaries via the first lens array from the measurement light irradiation unit via the second lens array.

Abstract: A capillary electrophoresis device includes: a plurality of capillaries; a light source; a detector that detects light transmitted through the capillaries; an optical coupling optical system and a plurality of first optical fibers provided between the light source and the plurality of capillaries; and a plurality of second optical fibers provided between the plurality of capillaries and the detector. The optical coupling optical system couples the light from the light source to the plurality of first optical fibers. Each of the plurality of first optical fibers has a first end face connected to the optical coupling optical system, and a second end face arranged close to and opposite to a corresponding capillary among the capillaries. Each of the plurality of second optical fibers has a first end face arranged close to and opposite to a corresponding capillary among the capillaries, and a second end face connected to the detector.

Abstract: The present invention aims to provide an electrophoresis apparatus which makes it possible to execute protein analysis with a high throughput. The electrophoresis apparatus according to the present invention is equipped with a capillary array which is configured by arraying a plurality of capillaries, a measurement light irradiation unit which irradiates with measurement light, a first lens array which includes a plurality of first lenses which are arrayed in correspondence with the plurality of capillaries, a second lens array which includes a plurality of second lenses which are arrayed in correspondence with the plurality of capillaries, and a light receiving unit which receives light which is incident upon the capillaries via the first lens array from the measurement light irradiation unit via the second lens array.

Abstract: In a right-handed XYZ coordinate system, a dichroic-mirror array of the present disclosure includes a first group in which m (m?2) dichroic mirrors DA1 to DAm are arranged parallel to each other along a positive direction of an X axis and a second group in which n (n?2) dichroic mirrors DB1 to DBn are arranged parallel to each other along a negative direction of the X axis. Incident surfaces of the DA1 to DAm and incident surfaces of the DB1 to DBn are perpendicular to an XZ plane. A slope of straight lines with normal lines of the incident surfaces of the DA1 to DAm projected onto the XZ plane are negative, and a slope of straight lines with normal lines of incident surfaces of DB1 to DBn projected onto the XZ plane are positive.

Abstract: An electrophoresis system includes a plurality of capillaries in which electrophoresis of a sample is performed, a light source that irradiates detection positions of the capillaries with light, a light detector that detects light generated by the irradiation with the light by the light source that depends on components contained in the sample, and a buffer storage unit into which one end of the plurality of capillaries are inserted at a time of the electrophoresis of the sample and in which a buffer is stored. A computer controls an electrophoresis condition of each of the plurality of capillaries such that arrival times for the components that move in the plurality of capillaries to reach the detection positions are shifted from each other. As a result, it is possible to detect fluorescence signals at different timings in each of the plurality of capillaries.

Abstract: A light-emission detection apparatus is provided for individually condensing light emitted from each emission point of an emission-point array using each condensing lens of a condensing-lens array to forma light beam and detecting each light beam incident on a sensor in parallel. The light-emission detection apparatus can be downsized and high sensitivity and low crosstalk can be simultaneously accomplished when a certain relation between the diameter of each emission point, a focal length of each condensing lens, an interval of condensing lenses, and an optical path length between each condensing lens and a sensor is satisfied.

Abstract: A light-emission detection apparatus is provided for individually condensing light emitted from each emission point of an emission-point array using each condensing lens of a condensing-lens array to forma light beam and detecting each light beam incident on a sensor in parallel. The light-emission detection apparatus can be downsized and high sensitivity and low crosstalk can be simultaneously accomplished when a certain relation between the diameter of each emission point, a focal length of each condensing lens, an interval of condensing lenses, and an optical path length between each condensing lens and a sensor is satisfied.

Abstract: The invention is directed to a light-emission detection apparatus for individually condensing light emitted from each emission point of a emission-point array using each condensing lens of a condensing-lens array to form a light beam and detecting each light beam incident on a sensor in parallel, and the light-emission detection apparatus can be downsized and high sensitivity and low crosstalk can be simultaneously accomplished when a certain relation between the diameter of each emission point, a focal length of each condensing lens, an interval of condensing lenses, and an optical path length between each condensing lens and a sensor is satisfied.

Abstract: In an analysis method and an analysis system for detecting fluorescences from each of a plurality of light-emitting points in a plurality of wavelength bands in order to identify fluorescence emissions of a plurality of types of fluorophores from the plurality of light-emitting points, spatial crosstalk and spectral crosstalk are present between the plurality of light-emitting points and between the plurality of wavelength bands, and then performance of the identification is reduced. The spatial crosstalk and the spectral crosstalk are eliminated and concentrations of each of the plurality of types of fluorophores at each of the plurality of light-emitting points are derived by inputting all detection signals in the plurality of wavelength bands for the plurality of light-emitting points to a predetermined arithmetic operation expression.

Abstract: A multicapillary electrophoresis device includes: a capillary array formed by arranging a plurality of capillaries; a light source irradiating the capillaries with excitation light; a photodetector detecting fluorescence from a sample in the capillaries; and an arithmetic control unit calculating a signal intensity of the fluorescence according to a signal from the photodetector. The arithmetic control unit is configured so as to correct the signal intensity according to a correction index determined for each of the combinations of any of the capillaries and a fluorophore labeled to the sample.