Abstract: Provided is a method by which a nucleic acid sample can be collected from a subject minimally invasively. A method for preparing a nucleic acid derived from a skin cell of a subject, comprising isolating the nucleic acid from skin surface lipids collected from the subject.

Abstract: Provided is a method for amplifying a nucleic acid from RNA with high sensitivity and a high yield. The method for amplifying a nucleic acid comprises: synthesizing cDNA from sample RNA through reverse transcription reaction; and amplifying the cDNA by multiplex PCR, wherein the reverse transcription and the multiplex PCR are performed in the presence of a single-stranded nucleic acid-binding protein, and the single-stranded nucleic acid-binding protein is T4GP32 or a mutant thereof.

Abstract: Provided are an internal reference gene for use in measuring an expression level of a target gene contained in a skin surface lipid specimen, and a method for measuring an expression level of a target gene contained in a skin surface lipid specimen using the internal reference gene. The present invention provides a method for measuring an expression level of a target gene contained in a skin surface lipid specimen, comprising using at least one gene selected from the group consisting of particular 52 genes as an internal reference gene in PCR.

Abstract: A method for preparing a nucleic acid sample derived from skin stratum corneum includes: extracting nucleic acid from stratum corneum collected from a skin surface to prepare a water-soluble alcohol aqueous solution containing the nucleic acid; contacting the nucleic acid in the water-soluble alcohol aqueous solution with a solid-phase material to separate the nucleic acid; and amplifying, or reverse-transcribing and amplifying, the separated nucleic acid in the presence of single-stranded nucleic acid-binding protein to prepare a nucleic acid sample.

Abstract: A vehicle body structure provided on a vehicle includes a floor pan and a bracket. On an upper surface of the floor pan, a cushion of a seat on which an occupant of the vehicle is to be seated is to be disposed. A bracket is disposed on an outer end side of a seating position for the occupant in a vehicle width direction of the vehicle on the upper surface of the floor pan, and includes at least one protruding portion protruding upward and extending in a front-rear direction.

Abstract: Provided is a method by which a nucleic acid sample can be collected from a subject minimally invasively. A method for preparing a nucleic acid derived from a skin cell of a subject, comprising isolating the nucleic acid from skin surface lipids collected from the subject.

Abstract: Provided are a marker for detecting the severity of atopic dermatitis and a method for detecting the severity of atopic dermatitis using the marker.

Abstract: Provided is a method for detecting childhood atopic dermatitis using skin surface lipids. The method for detecting childhood atopic dermatitis in a test subject comprises a step of measuring an expression level of TARC gene or an expression product thereof in skin surface lipids collected from the test subject.

Abstract: The present invention provides a method for preventing degradation of skin surface lipid-derived RNA, comprising treating a collection medium containing skin surface lipids with an aqueous solution which comprises at least one selected from the group consisting of guanidine hydrochloride and guanidine thiocyanate and in which water or an alcohol aqueous solution is used as a solvent.

Abstract: Provided are a marker for detecting the severity of atopic dermatitis, and a method for detecting the severity of atopic dermatitis using the marker. The method for detecting the severity of atopic dermatitis in a test subject comprises a step of measuring an expression level of at least one gene selected from the group of 9 genes consisting of CAPN1, NCOR2, PPP1R9B, PLP2, GRN, PPP1R12C, LOC146880, SLC31A1 and SYVN1 or an expression product thereof for a biological sample collected from the test subject.

Abstract: Provided is a method for collecting RNA contained in skin surface lipids (SSL). A method for preparing SSL-derived RNA. In this method, an aqueous solution containing RNA prepared from SSL of a subject is mixed with a water-soluble organic solvent, and the resulting mixture is allowed to contact with a solid phase material to separate RNA. By adjusting the concentration of the water-soluble organic solvent in the mixture, the yield of RNA improves.

Abstract: Provided are a marker for detecting atopic dermatitis, and a method for detecting atopic dermatitis using the marker. The method for detecting atopic dermatitis in a test subject comprises a step of measuring an expression level of a gene or an expression product thereof contained in a biological sample collected from the test subject.

Abstract: Provided is data processing of RNA information in order to perform effective normalization in the case of analyzing RNA information obtained from secretion derived from a subject.

Abstract: Provided are a marker gene for detecting Parkinson's disease, and a method for detecting Parkinson's disease by using the marker gene. The method for detecting Parkinson's disease in a test subject comprises a step of measuring an expression level of at least one gene selected from the group of 4 genes consisting of SNORA16A, SNORA24, SNORA50 and REXO1L2P or an expression product thereof in a biological sample collected from the test subject.

Abstract: Provided are a method of detecting severity of premenstrual syndrome simply and with less burdening a subject, a marker allowing detection of the severity of premenstrual syndrome, and a method of detecting the severity of premenstrual syndrome of a subject using the marker.

Abstract: A vehicle body structure provided on a vehicle includes a floor pan and a bracket. On an upper surface of the floor pan, a cushion of a seat on which an occupant of the vehicle is to be seated is to be disposed. A bracket is disposed on an outer end side of a seating position for the occupant in a vehicle width direction of the vehicle on the upper surface of the floor pan, and includes at least one protruding portion protruding upward and extending in a front-rear direction.

Abstract: Provided is a novel compound having a selective activating effect on ER?. The present invention provides a compound represented by the following formula (1) wherein R1 represents a cycloalkyl group having 3 to 8 carbon atoms, an alkenyl group having 2 to 6 carbon atoms and optionally substituted with a halogen atom, a 5-membered nitrogen-containing heteroaryl group, a 4- to 6-membered cyclic amino group, an alkanoylamino group having 2 to 6 carbon atoms and optionally substituted with a halogen atom, a 1-trifluoromethyl-1-hydroxymethyl group, or a 1-methylpropyl group; R2 to R5 are the same or different and each represent a hydrogen atom or a fluorine atom; and R6 represents a hydrogen atom or an alkanoyl group having 2 to 5 carbon atoms, or a salt thereof.

Abstract: Provided is a novel compound having a selective activating effect on ER?. The present invention provides a compound represented by the following formula (1) wherein R1 represents a cycloalkyl group having 3 to 8 carbon atoms, an alkenyl group having 2 to 6 carbon atoms and optionally substituted with a halogen atom, a 5-membered nitrogen-containing heteroaryl group, a 4- to 6-membered cyclic amino group, an alkanoylamino group having 2 to 6 carbon atoms and optionally substituted with a halogen atom, a 1-trifluoromethyl-1-hydroxymethyl group, or a 1-methylpropyl group; R2 to R5 are the same or different and each represent a hydrogen atom or a fluorine atom; and R6 represents a hydrogen atom or an alkanoyl group having 2 to 5 carbon atoms, or a salt thereof.

Abstract: Provided is a method for analyzing RNA of a subject with high accuracy. The present invention provides a method for preparing a nucleic acid derived from a skin cell of a subject, the method comprising preserving at 0° C. or lower an RNA-containing skin surface lipid collected from the subject; and a method for preparing a nucleic acid derived from a skin cell of a subject, the method comprising converting RNA has been contained in a skin surface lipid of the subject into cDNA, then amplifying the cDNA by multiplex PCR, and purifying the resulting reaction product.

Abstract: Provided is a method by which a nucleic acid sample can be collected from a subject minimally invasively. A method for preparing a nucleic acid derived from a skin cell of a subject, comprising isolating the nucleic acid from skin surface lipids collected from the subject.