Abstract: A pharmaceutical composition containing cariprazine, a preparation method and an application thereof are provided. The pharmaceutical composition contains cariprazine pamoate solid particles. A particle size of the cariprazine solid particles has a Dv(10) less than or equal to 30 microns, a Dv(50) less than or equal to 50 microns, and a Dv(90) less than or equal to 100 microns. When being used, the pharmaceutical composition containing cariprazine is an aqueous suspension and, the concentration of the free base of cariprazine is moderate. A long-term effect can be achieved upon injection of a certain administration volume of the pharmaceutical composition, thereby reducing the frequency of medication while increasing patient medication compliance. Furthermore, the pharmaceutical composition has a high bioavailability and has promising marketing prospects.

Abstract: A pharmaceutically acceptable salt of cariprazine, a preparation method therefor, a pharmaceutical composition containing same, and the use thereof are provided. The pharmaceutically acceptable salt of cariprazine is a salt formed from the free base of cariprazine and an organic acid having six or more carbon atoms, and has relatively good solubility and stability.

Abstract: Provided are a cell strain HEK293.CS for reducing the production of a replication competent adenovirus, and a construction method and the use thereof. HEK293.CS is a safe adenovirus-producing cell line constructed by knocking out a gene fragment homologous to the Ad5 adenovirus E1 gene in HEK293 and providing a template plasmid to replace said gene fragment with a non-homologous sequence that stabilizes the expression of the E1 gene. Compared with the unmodified HEK293 cell strain, HEK293.CS shows no decrease in growth ability and virus production ability, but does not produce a detectable RCA. HEK293.CS can be used for the mass culture of a recombinant human type 5 adenovirus, and reducing the probability of RCA production in the manufacture process of drugs such as vaccines and antibodies.

Abstract: Provided is a method for preparing an adenovirus vector vaccine by means of a perfusion culture process. The method comprises a step of culturing adenovirus host cells, and in particular a step of adjusting the perfusion rate by means of at least two stages according to cell density. The method increases the single cell yield of a virus after infection and the specific activity of a virus harvest liquid while achieving high-density growth of adenovirus host cells.

Abstract: Provided are an mRNA or an mRNA composition, and an mRNA vaccine comprising the mRNA or the mRNA composition. The mRNA or the mRNA composition comprises an mRNA sequence encoding an S protein of a novel coronavirus SARS-CoV-2 or a variant thereof, and an mRNA sequence encoding an RBD in the S protein or a variant thereof. Further provided are the applications of the mRNA or the mRNA composition, and the mRNA vaccine comprising the mRNA or the mRNA composition in preparation of a medication for preventing and/or treating a disease caused by a novel coronavirus SARS-CoV-2 infection.

Abstract: An on-chip ultra-narrow linewidth laser and a method for obtaining a single-longitudinal mode ultra-narrow linewidth optical signal are provided in the present invention.

Abstract: Provided is a determination method of differential protection suitable for an electricity transmission line with multi-terminal T-connection.

Abstract: The present disclosure provides a noble metal-transition metal-based nano-catalyst thin film and a preparation method thereof, belonging to the fields of energy development and pollutant emission reduction. Based on a micro-nano processing technology, a noble metal-transition metal-based nano-catalyst thin film is loaded on a semi-cylindrical pipe with an inner thread structure, and heat generated is quickly accumulated on an upper surface of the catalyst to establish a large temperature gradient. By the insulation and high roughness of an alumina carrier layer and the inner thread structure of the pipe, a catalyst loading area is maximized and dispersion of noble metal atoms is enhanced. A transition metal-transition metal oxide thin film protects a noble metal nano-catalyst by core-shell wrapping, and a transition metal oxide prevents catalyst deactivation caused by oxygen occupying too many metal active sites.

Abstract: An endoscope locking device comprises: a pedestal, a lock buckle and an elastic element, the pedestal is provided with a slot for mounting the lock buckle, the lock buckle is provided with an oblong hole in the center, and the lock buckle is mounted within the slot of the pedestal, and the lock buckle may reciprocate within the slot; and the lock buckle and the pedestal are elastically connected by the elastic element. The endoscope is fixed by using the elasticity of the elastic rubber ring to the endoscope locking device formed by the lock buckle and the pedestal.

Abstract: The present disclosure provides a method and a device for control of blockchain data access based on a smart contract. The method includes obtaining, by a blockchain node, an access operation request of an institution, the access operation request including a transaction identifier of a transaction to be accessed, according to the transaction identifier, determining a blockchain state corresponding to the transaction identifier, the blockchain state including a hash value of the transaction corresponding to a last time change of the blockchain state, according to the hash value of the transaction corresponding to the last time change in the blockchain state and an identifier of the institution, performing data access control on the institution, obtaining control result, and transmitting the control result to the institution.

Abstract: Provided are a protein antigen subjected to site-directed mutation and site-directed modification, and a method for site-directed mutation and site-directed modification of the protein antigen. The method comprises: site-directedly introducing an unnatural amino acid into a specific site of the protein antigen by genetic codon expansion technique; and performing site-directed modification with the protein antigen by the unnatural amino acid and a modifier, wherein the modifier is a receptor agonist such as tripalmitoyl-S-glyceryl cysteine and monophosphoryl lipid A. Further provided is use of the protein antigen subjected to site-directed mutation and site-directed modification, such as use as a vaccine.

Abstract: A hydraulic steering system, comprising a steering pump and a plurality of digital steering cylinder assemblies Each digital steering cylinder assembly includes a steering cylinder and a digital hydraulic valve configured to selectively communicate a rodless cavity of the steering cylinder with one of the steering pump and a return oil circuit, and communicate a rod cavity of the steering cylinder with the other of the steering pump and the return oil circuit.A controller can be configured to emit a digital pulse signal to a motorand cause the digital hydraulic valve to move towards a first working position or a second working position andA mechanical feedback structure can be configured to be driven by a piston rod of the steering cylinder when the valve core of the digital hydraulic valve moves towards the first working position or the second working position, so as to drive the digital hydraulic valve to return to a neutral position.

Abstract: Disclosed is a SARS-CoV-2 vaccine, wherein the S protein of SARS-CoV-2 serves as the antigen, and the vaccine comprises an adenoviral vector, and the vaccine induces an improved protective immune response through mucosal immunity, thus preventing SARS-CoV-2 infection. Specifically, when atomized by an appropriate apparatus, the vaccine generates particles of improved uniformity, which can reach the lungs after being inhaled via the nasal cavity or the oral cavity, thus producing a protective immune response with respect to the entire respiratory tract and the lungs, enhancing the effective utilization rate of the vaccine, and increasing the effect of the vaccine.

Abstract: A method for authorizing a blockchain data access on a blockchain platform includes: obtaining from a third-party institution a data access request containing a user ID of data access, a data scope, and a random number of a signature, which is generated by the third-party institution after receiving a random number sent by a client terminal and signing on the received random number; performing a verification on the random number of the signature and the data scope; and after the verification is passed, sending data ciphertext corresponding to the user ID to the third-party institution, such that the third-party institution decrypts the data ciphertext using a data secret key sent by the client terminal.

Abstract: The present invention relates to a carrier protein with site-directed mutation and use thereof in preparation of a vaccine, wherein the carrier protein is selected from fusion proteins formed by one, two or more of diphtheria toxoid, a non-toxic mutant of diphtheria toxin, a bacterial outer membrane protein and a bacterially expressed protein, wherein an amino acid at at least one site on the carrier protein is mutated into an unnatural amino acid, and the unnatural amino acid contains an azido or alkynyl terminal group. In a mutual reaction process of the carrier protein with site-directed mutation of the present invention and a polysaccharide antigen, a covalent bond is formed, and meanwhile a formed conjugate is in a bead-string state, so that the carrier protein and the polysaccharide antigen can be effectively prevented from being excessively crosslinked.

Abstract: A display substrate includes a base substrate; a gate metal pattern including a gate electrode of a thin film transistor and gate lines; a source-drain metal pattern including a source electrode and a drain electrode of the thin film transistor, and data lines, where the gate line cross the data line, to define a plurality of pixel regions arranged in an array form; and a first transparent metal pattern including a common electrode pattern. A minimum distance between each gate line and common electrode patterns in a row of pixel regions located in a same row as the gate line in a first direction is a first spacing, a minimum distance between the gate line and common electrode patterns in the other row of pixel regions adjacent to the gate line in the first direction is a second spacing, and the first spacing is greater than the second spacing.

Abstract: An atrial shunt regulation device, a braid tool and a braid method thereof. The left disk (1) and the right disk (2) of the atrial shunt regulation device are connected via the intermediate portion (3) as a single piece, and the left disk (1), the intermediate portion (3) and the right disk (2) are braided by a single braid wire (10). The ends of the braid wires are secured by one nut in the single-braid method, which reduces the number and size of the nuts and easily forms a cortical layer. The braid tool for braiding an atrial shunt regulation device comprises a cylinder braid body mold (20), the cylindrical braid body mold (20) includes positioning pins (21) that are evenly connected to an outer wall of the braid body mold. The braid method of the atrial shunt regulation device comprises braiding, thermoforming, braiding a binding-off wire, securing ends of wires by a nut, and thermoforming again.

Abstract: The present disclosure relates to the field of ecological restoration of a coal gangue hill, and in particular, to a solid waste-based porous material, a method for preparing the solid waste-based porous material, and a method of ecological restoration of the coal gangue hill by applying the solid waste-based porous material. A coal-based solid waste restoration material and mycorrhizal solid bacterial agent are mixed to restore the coal gangue hill, the coal-based solid waste restoration material is prepared by mixing coal-based solid waste porous materials, low-rank coal, and waste organic matter and adding a microbial quickly decomposition agent for aerobic fermentation and standing.

Abstract: An adjustable endoscope fixing device, comprises a tail bed, a three-way connector and a locking screw; an outer wall surface of a straight column section of the tail bed is provided with a rectangular slot; and an outer wall surface of a first connecting end of the three-way connector is provided with a locking structure, and the straight column section of the tail bed is connected to the first connecting end of the three-way connector, and the tail bed is fixed to the three-way connector by the locking structure.

Abstract: Provided is a novel coronavirus vaccine using replication-deficient human type 5 adenovirus as a vector. The vaccine takes the replication-deficient human type 5 adenovirus that is lack of E1 and E3 in a combined mode as a vector, and HEK293 cells that integrate adenovirus E1 genes serve as a packaging cell line, and protective antigenic genes carried are optimized COVID-19 (SARS-CoV-2) S protein genes (Ad5-nCoV). The vaccine has good immunogenicity in both mouse and guinea pig models and can induce the body to produce a strong cellular and humoral immune responses in a short time. Research on the protective effect of hACE2 transgenic mice shows that 14 days after a single Ad5-nCoV immunization, the viral load in lung tissues can be significantly reduced. It shows that the vaccine has a good immune protection effect against COVID-19.