Abstract: There is provided a particle sorting kit including a filter unit, which has a small amount of particle loss at a connection portion between a tube through which a sample liquid flows and the filter unit and functions even at a low flow rate. There is provided a particle sorting kit including a sample accommodation unit for accommodating a sample liquid containing particles, a sample flow path through which the sample liquid flows, a detection region in which a target particle is detected from the sample liquid, and a filter unit including a filter and a fitting portion that fits to an outer diameter of a tube for connection with the sample accommodation unit and/or the sample flow path, in which the fitting portion includes a protrusion that protrudes in the tube direction.

Abstract: To provide a technology of optimizing a suction condition of a microparticle. The present technology provides an optimizing method of a suction condition of a microparticle including: a particle number counting step of detecting a time point when a microparticle passes through a predetermined position on a main flow path through which liquid containing the microparticle flows, sucking the microparticle from the main flow path to a microparticle suction flow path by the microparticle suction flow path with a predetermined suction force, and counting the number of microparticles sucked into the microparticle suction flow path; and a step of determining an elapsed time from passage through the predetermined position with which the suction by the microparticle suction flow path should be performed on the basis of a time from the time point when the microparticle passes through the predetermined position on the main flow path until the suction is performed and the number of counted microparticles.

Abstract: There is provided a microparticle sorting method including a procedure of collecting a microparticle in a fluid that flows through a main channel in a branch channel that is in communication with the main channel by generating a negative pressure in the branch channel. In the procedure, a flow of a fluid is formed that flows toward a side of the main channel from a side of the branch channel at a communication opening between the main channel and the branch channel.

Abstract: To provide a technology of optimizing a suction condition of a microparticle. The present technology provides an optimizing method of a suction condition of a microparticle including: a particle number counting step of detecting a time point when a microparticle passes through a predetermined position on a main flow path through which liquid containing the microparticle flows, sucking the microparticle from the main flow path to a microparticle suction flow path by the microparticle suction flow path with a predetermined suction force, and counting the number of microparticles sucked into the microparticle suction flow path; and a step of determining an elapsed time from passage through the predetermined position with which the suction by the microparticle suction flow path should be performed on the basis of a time from the time point when the microparticle passes through the predetermined position on the main flow path until the suction is performed and the number of counted microparticles.

Abstract: To provide a microchip that is easily handled. Provided is a microchip having a plate shape and including: a sample liquid inlet into which a sample liquid is introduced; a main flow path through which the sample liquid introduced from the sample liquid inlet flows; and a sorting flow path into which a target sample is sorted from the sample liquid, in which the sample liquid inlet and a terminal end of the sorting flow path are formed on a same side surface. Furthermore, a sample sorting kit including the microchip is also provided. Moreover, a microparticle sorting device on which the microchip is mounted is also provided.

Abstract: Provided is a new method for more efficiently generating emulsion particles each containing one fine particle.

Abstract: To provide a technology of optimizing a suction condition of a microparticle. The present technology provides an optimizing method of a suction condition of a microparticle including: a particle number counting step of detecting a time point when a microparticle passes through a predetermined position on a main flow path through which liquid containing the microparticle flows, sucking the microparticle from the main flow path to a microparticle suction flow path by the microparticle suction flow path with a predetermined suction force, and counting the number of microparticles sucked into the microparticle suction flow path; and a step of determining an elapsed time from passage through the predetermined position with which the suction by the microparticle suction flow path should be performed on the basis of a time from the time point when the microparticle passes through the predetermined position on the main flow path until the suction is performed and the number of counted microparticles.

Abstract: To provide a particle sorting kit provided with a filter that functions even at a small flow rate with a small loss amount of particles. Provided is a particle sorting kit provided with a sample accommodation unit configured to accommodate a sample liquid containing particles, a microchip provided with a sample flow path through which the sample liquid flows and a sorting flow path in which target particles are sorted from the sample liquid, and a filter unit provided with a filter and a tapered portion that decreases a flow path diameter in a flow direction downstream of the filter.

Abstract: A microparticle sorting microchip for a flow cytometer is provided to enable sorting of microparticles at higher speed, higher purity, and higher acquisition rate. The microparticle sorting microchip includes a main channel through which a microparticle-containing fluid flows, a trap channel coaxially communicating with the main channel, a trap chamber communicating with the trap channel, and a gate channel intersecting the trap channel. The trap channel has an opening intersecting the gate channel. The trap channel has a smaller cross-sectional area upstream of the opening than downstream of the opening along a direction in which the microparticle-containing fluid flows.

Abstract: There is provided a microchip comprising: a main flow path through which a liquid containing microparticles flows; and a branch flow path that branches from the main flow path. A cross-sectional area of a portion of the main flow path is substantially constant up to a branch start position or decreases toward the branch start position, and a radius of curvature R of a side wall that connects a side wall of the main flow path and a side wall of the branch flow path is 0.5 mm or less and more than 0 mm.

Abstract: A microchip is provided, which includes a substrate including a fluid channel structure. The fluid channel structure includes a first fluid introduction channel and a second fluid introduction channel configured to meet so as to allow merging of a first fluid introduced from the first fluid introduction channel and a second fluid introduced from the second fluid introduction channel. A tapered portion is configured to be positioned after merging the first fluid and the second fluid so as to suppress a spiral flow field generated after the merging.

Abstract: There is provided a microchip comprising: a main flow path through which a liquid containing microparticles flows; and a branch flow path that branches from the main flow path. A cross-sectional area of a portion of the main flow path is substantially constant up to a branch start position or decreases toward the branch start position, and a radius of curvature R of a side wall that connects a side wall of the main flow path and a side wall of the branch flow path is 0.5 mm or less and more than 0 mm.

Abstract: A particle sorting apparatus is provided. The particle sorting apparatus includes a first detector of a first scatter channel to detect a first light scatter generated by a particle flowing in a flow channel of a fluid sample and passing through a first laser beam, and a second detector of a second scatter channel to detect a second light scatter generated by the particle flowing in the flow channel of the fluid sample and passing through a second laser beam. The particle sorting apparatus also includes circuitry configured to output a first scatter pulse information and a first detection time of the first light scatter channel, a second scatter pulse information and a second detection time of the second light scatter channel. The first laser beam and the second laser beam are irradiated to the particle in different positions of the flow channel.

Abstract: A microchip is provided, which includes a substrate including a fluid channel structure. The fluid channel structure includes a first fluid introduction channel and a second fluid introduction channel configured to meet so as to allow merging of a first fluid introduced from the first fluid introduction channel and a second fluid introduced from the second fluid introduction channel. A tapered portion is configured to be positioned after merging the first fluid and the second fluid so as to suppress a spiral flow field generated after the merging.

Abstract: There is provided a microparticle sorting method including a procedure of collecting a microparticle in a fluid that flows through a main channel in a branch channel that is in communication with the main channel by generating a negative pressure in the branch channel. In the procedure, a flow of a fluid is formed that flows toward a side of the main channel from a side of the branch channel at a communication opening between the main channel and the branch channel.

Abstract: In the present technology, the timing at which suction is performed is optimized in order to enhance the microparticle separation performance in a technology for separating target microparticles in a microchip. For this purpose, the present technology provides a method of optimizing a microparticle suction condition, and the like, using a microchip having a main flow channel through which a liquid containing a microparticle flows, a microparticle suction flow channel arranged coaxially with the main flow channel, and a branch flow channel branching from the main flow channel. The method includes: a branch point specifying process of specifying a branch point at which the branch flow channel branches from the main flow channel; and a time assignment process of assigning a time T1 to be applied to suction of the microparticle.

Abstract: Provided is a technology for preparing a mixture including multiple types of microparticles in accordance with a predetermined ratio. The present technology provides a microparticle sorting device (100) including a control unit (103) comprising a determination unit that determines whether microparticles are sorted, on the basis of light generated by irradiating, with light, the microparticles flowing through a flow channel (155), in which the determination unit performs a primary sorting determination to determine, on the basis of characteristics of the light generated, whether the microparticles belong to any one of two or more different microparticle populations, and then performs a secondary sorting determination to determine whether the microparticles determined to belong to any one of the microparticle populations in the primary sorting determination are sorted, on the basis of the particle constituent ratio specified for the two or more different microparticle populations.

Abstract: There is provided a microparticle sorting method including a procedure of collecting a microparticle in a fluid that flows through a main channel in a branch channel that is in communication with the main channel by generating a negative pressure in the branch channel. In the procedure, a flow of a fluid is formed that flows toward a side of the main channel from a side of the branch channel at a communication opening between the main channel and the branch channel.

Abstract: A particle sorting apparatus is provided. The particle sorting apparatus includes a first detector of a first scatter channel to detect a first light scatter generated by a particle flowing in a flow channel of a fluid sample and passing through a first laser beam, and a second detector of a second scatter channel to detect a second light scatter generated by the particle flowing in the flow channel of the fluid sample and passing through a second laser beam. The particle sorting apparatus also includes circuitry configured to output a first scatter pulse information and a first detection time of the first light scatter channel, a second scatter pulse information and a second detection time of the second light scatter channel. The first laser beam and the second laser beam are irradiated to the particle in different positions of the flow channel.