Abstract: The present invention provides a method for measuring concentration of a solution, in which an acid is mixed in a sample to be detected to reduce the variations in pH, and the mixture is heated up to not more than 80° C. to measure the transmitted light and/or scattered light power. The present invention also provides a method of urinalysis in which the protein concentration is measured after measuring the angle of rotation. Herewith, in the method in which the sample to be detected is heated to coagulate protein, and the protein concentration is measured from the degree of opacification resulting therefrom, it is possible to reduce the influence of the pH of the sample to be detected, and to decrease the heating temperature.

Abstract: A blood processing method according to the present invention includes Step St1 of preparing a blood specimen sample and a hemolyzing agent, and Step St2 of mixing the blood specimen sample and the hemolyzing agent to prepare a mixture sample. In this case, the hemolyzing agent is a solid reagent for destroying erythrocyte membranes.

Abstract: In Step St1, a reaction system including a substance to be measured or a specific binding substance is constructed and measurement of an optical change amount is started. In Step St2, the specific bonding substance and the substance to be measured are mixed and changes with time in the optical change amount of the reaction system are measured. In Step St3, a time interval including a time when an increase rate of the optical change amount in measurement data is the maximum is selected. In Step St4, discrete Fourier transform is performed, with the selected time interval assumed to be a period of a periodic function, to the measurement data for the optical change amount, thereby obtaining an amplitude spectrum distribution. In Step St5, by comparing the shape of the amplitude spectrum distribution to a reference shape, whether a zone phenomenon has occurred in the reaction system is judged.

Abstract: According to the present invention, the expansion of measurable concentration range for an antigen in a sample solution can be achieved, without a step of dilution or the like. The concentration of an antigen contained in a sample solution is determined from the maximum value and/or minimum value of turbidity level detected in the observation of the transient phenomenon of turbidity, elapsed time when the maximum and/or minimum value is observed, and turbidity level.

Abstract: A fluorescence polarization assay is a method for measuring a subject substance, which includes the steps of: (a) preparing a first solid support body on which a binding substance capable of specifically binding to the subject substance is immobilized and a fluorescent labeled substance which is capable of specifically binding to the subject substance and is labeled with a fluorescent dye; (b) bringing the fluorescent labeled substance and the binding substance into contact with the subject substance; and (c) measuring the polarization degree of fluorescent light from the fluorescent labeled substance.

Abstract: In order to simplify the pretreatment such as collection of an analyte, treatment of the analyte, introduction of the analyte into a measurement system (reaction system) and dilution of the analyte and to improve operability in examinations using large-sized automatic analyzers and POCT devices, the present invention provides an analyte sampling element including a first region capable of quantitatively collecting and temporarily retaining the analyte and a second region on which a dynamic effect is acted from the outside to move the first region.

Abstract: As shown in FIG. 1, an immunochromatography test strip 10 of the present embodiment is made of a base material 11 which includes a sample introduction section 12, a labeling section 13 and an immobilization section 14. The base material 11 is made of a material that is capable of being impregnated with a solvent of a sample solution containing a detection target substance. The material allows migration of a solvent of a sample solution by capillary action. The sample introduction section 12 is a region where a sample solution containing a detection target substance is introduced (e.g., dripped). The sample introduction section 12 contains a metal salt supported thereon. The labeling section 13 contains a labeled antibody 15, which is labeled with a labeling substance, in a state such that the labeled antibody 15 can be eluted into the sample solution. The labeled antibody 15 used herein specifically binds to a detection target substance. The immobilization section 14 contains an immobilized antibody 16.

Abstract: In order to improve the reliability of the measurement by judging the precision of a measured value and judging the effectiveness of the measurement based on a result of the precision judgment, the optical characteristic of a reagent solution is previously measured in measuring the concentration of a specific component in a sample solution. Consequently, the precision of the concentration measurement is ensured based on the degree of degradation of the reagent solution.

Abstract: An object of the present invention is provide a means capable of enlarging the measurable concentration range of a specific component in a solution to be detected, and further measuring a precise solution concentration with ease even when there occur inhibitors such as contamination of a sample cell, turbidity of the solution to be detected, and suspending particles. For achieving this object, the transmitted light intensities and/or the scattered light intensities of the solution to be detected before and after mixing a reagent for changing the optical characteristics of the solution to be detected attributed to the specific component are measured to obtain the concentration of the specific component in the solution to be detected from these measured values.

Abstract: A specific coupling reaction measuring method of this invention for measuring a content of a subject substance in a sample includes the steps of (a) constructing a reaction system including the sample and a magnetic particle on which a specific coupling substance for specifically coupling with the subject substance is immobilized; (b) measuring an optical characteristic of the reaction system; and (c) removing, from the reaction system, an agglutination complex including the subject substance, the specific coupling substance and the magnetic particle by utilizing magnetic force.

Abstract: In order to perform a qualitative or quantitative analysis of an analyte in a sample in a simple, rapid and accurate manner where the sample can be automatically diluted without the need for an advanced device or operation, under little influence attributed to the analyte concentration in the sample, a strip is used which comprises at least two or more units arranged therein, each unit comprising: a space forming zone 2; a retention zone 5 in which a labeled first specific binding substance is retained; and a detection zone 6 in which a second specific binding substance is immobilized, so that the analyte in the sample is qualitatively or quantitatively analyzed based on a signal intensity obtained attributed to a specific binding reaction in the detection zone 6.

Abstract: Measurement of the concentration of a specific component in a test liquid is made more efficient by obtaining a mixed liquid of the test liquid and a reagent liquid without having to perform a stirring operation. A mixed liquid comprising the test liquid and the reagent liquid is obtained by injecting the reagent liquid into the test liquid with stirring caused by the injection; and with the test liquid being irradiated with light, at least a first optical characteristic of the test liquid before the injection of the reagent liquid and a second optical characteristic of the test liquid after the injection of the reagent liquid are measured to measure the concentration of the specific component in the test liquid from the first optical characteristic and the second optical characteristic.

Abstract: The immunoassay method of this invention measures the content of a subject substance in a sample. The method includes the steps of: (a) preparing a mixed solution by mixing the sample and an antibody solution including a first monoclonal antibody and a second monoclonal antibody capable of specifically binding to the subject substance; and (b) measuring an optical property of the mixed solution. The first monoclonal antibody is capable of binding to a first epitope of the subject substance, and the second monoclonal antibody is capable of binding to a second epitope of the subject substance different from the first epitope. Each of the first and second epitopes exists singly in the subject substance.

Abstract: The present invention provides a controlling method of optical property measurement system wherein optical properties of a liquid sample are measured by projecting first light to analyze transmitted light, the method comprising the steps of projecting additional light to a path of the first light or in a periphery thereof to detect the presence or absence of bubbles and/or particles which may interfere with transmission of the first light, based on an intensity of transmitted light of the second light, and removing the bubbles and/or particles when their presence is confirmed. This eliminates the need of detaching the sample cell from an optical system to introduce or excrete the sample and facilitates precise measurement of optical properties of sample even when interfering substances are present in the sample cell.

Abstract: A specific bonding analysis method by which sensitivity and concentration range in analysis can be freely set, and a device using this are provided. The intended analysis is optimized by using a specific bonding analysis device capable of controlling the velocity of a sample passing through a detection part and causing no remaining of unnecessary components on a detection part.

Abstract: According to the present invention, in a polarimetry for allowing a light to be incident upon a specimen with a spontaneous optical active substance, a polarized light is allowed to be incident upon the specimen while micro-vibrating the plane of vibration; a component having a specific plane of vibration is detected out of the polarized light through the specimen; a component with a certain angular frequency is extracted; and an angle of rotation attributed to the specimen is calculated based on 3 or more groups of data including a relative angle formed between the plane of vibration of the light incident upon the specimen and the plane of vibration of the light, and an intensity of the component with the angular frequency obtained at the relative angle. Consequently, the influence of noises mixed due to bubbles, particles and the like can be eliminated, thereby achieving a stable and highly accurate measurement in a shorter time.

Abstract: Disclosed are a method of stirring a solution which involves injecting a gas into a sample solution in order to perform stirring without using a stirring rod or the like when mixing the sample solution with a reagent solution, as well as a sample cell provided with an inlet for injecting a gas into a sample solution and an apparatus for measuring a concentration of a solution employing the same.

Abstract: In order to provide a specific binding analysis method capable of quantitatively or qualitatively determining an analyte in a sample in a simple, rapid and accurate manner with little influence of the prozone phenomenon, background and foreign matter, and a specific binding analysis apparatus used therefor, a database relating to a signal intensity attributed to a specific binding reaction is previously prepared for an individual sample containing a suspected analyte, a measurement pattern of a signal intensity of the sample is determined based on the database, and the concentration of the analyte in the sample is further determined.

Abstract: Disclosed is a specific binding analysis method capable of controlling the amount of specific binding to freely setting the sensitivity, concentration range and the like in an analysis, and a device used therefor. In order to optimize the amount of specific binding, the specific binding analysis method and the device used therefor restrain the amount and velocity of a sample passing, by capillarity, through a detection zone, by controlling the dimensions, ventilation resistance, hydrophilicity and the like in the portion of the device where the sample passes through.

Abstract: To enable accurate qualitative and quantitative analyses of an analyte in a sample without being influenced by a prozone phenomenon, there is disclosed a specific binding method including the steps of: bringing into contact a sample with a zone where a first specific binding substance labeled with a labeling material is retained, to allow an analyte to bind to the first specific binding substance; bringing into contact the sample with a first detection zone where a second specific binding substance is immobilized, to allow an excess of the analyte to bind to the second specific binding substance; bringing into contact the sample with a second detection zone where a substance identical to the analyte is immobilized, to allow the first specific binding substance to bind to the substance; respectively measuring the intensities of signal 1 and signal 2 obtained in the first detection zone and the second detection zone and attributed to the labeling material; and then determining the concentration of the analyte b