Abstract: A method of pipetting comprises (a) providing a pipette tip holder having a plurality of segregated compartments and a tip support member for supporting a plurality of pipette tips in the pipette tip holder, the tip support member configured to support a single pipette in tip each segregated compartment; (b providing a plurality of pipette tips in the tip support member; (c) removing a first pipette tip from a first segregated compartment; (d) using the first pipette tip to complete a pipetting operation and placing the first pipette tip into a segregated compartment that has not contained a used pipette tip; (e) subsequently removing the first pipette tip from the segregated compartment and using the first pipette tip to complete another pipetting operation; and, (f) replacing the first pipette tip into the pipette tip holder, whereby the first pipette tip, when placed in the segregated compartment, is segregated in the pipette tip holder to reduce the possibility of a fluid from the first pipette tip contac

Abstract: The present invention is a system of emergency vehicle lighting that uses a low profile hood/trunk mounted LED plate unit and a door mounted LED strip unit. It is designed for both stand alone operation or for integration into an emergency vehicle's electronic alarm notification sequencing unit. The hood/trunk LED plate unit has a thin, enhanced geometrical configuration and an LED lens design that allows the light emitted from the superbright LEDs to be seen to a much higher degree to vehicles approaching from direction normal to the longitudinal centerline of the emergency vehicle. Mounting of the LED plate unit and LED strip unit can be permanent or temporary and because of the light weight, numerous chemical, magnetic and mechanical methods of affixation may be utilized.

Abstract: A system and method for notifying primary and second drivers about a status of a safety restraint in a vehicle is provided. A primary and secondary keys are adapted to be associated to the primary and secondary drivers. A key ignition device is positioned on each of the primary key and secondary keys. The key ignition device is adapted to generate driver status signals indicative of whether the driver the primary driver or the secondary driver. A controller is adapted to determine whether the driver of the vehicle is the primary driver or the secondary driver based on the driver status signals. The controller is further adapted to generate restraint status signals indicative of the status of the safety restraint. The controller is further adapted to selectively control the operation of generating the restraint signals based on whether the driver of the vehicle is the primary or secondary driver.

Abstract: A system and method for controlling the operation of stabilizing the movement of a vehicle between primary and secondary drivers is provided. A primary key is adapted to be associated to the primary driver. A secondary key is adapted to be associated to the secondary driver. A key ignition device is positioned on the primary and secondary keys and is configured to generate driver status signals indicative of whether the driver is a primary or secondary driver. The controller is adapted to determine whether the driver of the vehicle is the primary driver or the secondary driver based on the driver status signals. The controller is further adapted to stabilize the movement of the vehicle. The controller is further adapted to selectively control the operation of stabilizing the movement of the vehicle in response to the driver status signals.

Abstract: A system and method is provided for controlling the operation of notifying drivers of the fluid level. Primary and secondary keys are adapted to be associated to primary and secondary drivers. A key ignition device is positioned on the primary and secondary keys and generate driver status signals. A fluid level sensing device is operable to generate a fluid level signal indicative of the fluid level. A controller is coupled to the fluid level sensing device and the key ignition device. The controller determines whether the driver of the vehicle is the primary or secondary driver in response to the driver status signals. The controller notifies the primary or secondary driver of the fluid level in response to the fluid level signal. The controller selectively controls the operation of notifying the primary or the secondary driver based on whether the driver is the primary or secondary driver.

Abstract: Nitrosamines are reduced in tobacco by treating a whole tobacco leaf with ferulic acid. Treatment is conducted by applying an aqueous solution of ferulic acid to the whole tobacco leaf immediately before, at, or after harvesting, or fire curing the whole tobacco leaf with wood smoke containing the ferulic acid.

Abstract: Linoleic acid is converted into ?-linolenic acid by the enzyme ?6-desaturase. The present invention is directed to isolated nucleic acids comprising the ?6-desaturase gene. More particularly, the isolated nucleic acid comprises the promoter, coding region and termination regions of the ?6-desaturase gene. The present invention provides recombinant constructions comprising the ?6-desaturase coding region in functional combination with heterologous regulatory sequences. The nucleic acids and recombinant constructions of the instant invention are useful in the production of GLA in transgenic organisms.

Abstract: Improved colony forming cell (CFC) assays are described. The improved assay comprises modifications to the standard CFC assay that enable analysis of temporal, real-time changes in antigen expression during colony development without need to fixate or destroy the culture. The improved assay is applicable to hematopoietic CFC assays as well as to CFC assays for other cells, such as neural cells and mammary cells. In one embodiment, the invention comprises adding a detection reagent, most likely a fluorescently labeled antibody, that is specific for antigens (most likely a cell-surface antigen) expressed on progenitors or on specific mature cell types to a culture at the start or during culture. The invention also comprises modifications to the culture medium and cell preparations used in standard CFC assays to selectively promote the development of one colony type while preventing or suppressing the development of other colony types.

Abstract: A pipette tip holder is provided for holding a plurality of pipette tips in an automated pipette machine. The pipette tip holder includes a container that defines a cavity. There is at least one vertical divider in the cavity that divides the cavity into a plurality of segregated compartments. The pipette tip holder further includes a tip support member. The tip support member is connected to the container, The tip support member defines a plurality of apertures. The apertures are sized to receive and suspend said pipette tips in the cavity. Each aperture is generally aligned with one of said compartments. The at least one vertical divider is sized and positioned to inhibit contact between bottom portions of adjacent pipette tips.

Abstract: The present invention is directed to a promoter derivable from an Arabidopsis ENDO gene. Nucleic acid constructs, vectors, plant cells and transgenic plants comprising the promoter are also provided.

Abstract: The invention is directed to isolated promoters from stem-regulated, defense-inducible genes, such as JAS promoters. The promoters are useful in expression cassettes and expression vectors for the transformation of plants. Particularly, the invention provides transgenic plants of rice and sugarcane that have been modified such that expression of a heterologous coding sequence is directed by an JAS promoter and is limited to stem tissues or may be upregulated by the presence of a defense-inducing agent. The invention also discloses methods for producing the expression vectors and transgenic plants.

Abstract: The present invention is directed to a promoter derived from an Arabidopsis ATP sulfurase gene. Nucleic acid constructs, vectors, plant cells and transgenic plants comprising the promoter are also provided.

Abstract: An improved magnetic target separation method is described. In particular, the magnetic target separation method can be used to separate target entities from non-target entities in a sample contained in a vessel without needing to remove the vessel from the magnetic field gradient.

Abstract: The present invention is directed to a promoter derivable from and Arabidopsis ENDO gene. Nucleic acid constructs, vectors, plant cells and transgenic plants comprising the promoter are also provided.

Abstract: The invention is directed to isolated promoters from stem-regulated, defense-inducible genes, such as JAS promoters. The promoters are useful in expression cassettes and expression vectors for the transformation of plants. Particularly, the invention provides transgenic plants of rice and sugarcane that have been modified such that expression of a heterologous coding sequence is directed by an JAS promoter and is limited to stem tissues or may be upregulated by the presence of a defense-inducing agent. The invention also discloses methods for producing the expression vectors and transgenic plants.

Abstract: The invention is directed to isolated promoters from stem-regulated, defense-inducible genes, such as OMT promoters. The promoters are useful in expression cassettes and expression vectors for the transformation of plants. Particularly, the invention provides transgenic plants of rice and sugarcane that have been modified such that expression of a heterologous coding sequence is directed by an OMT promoter and is limited to stem tissues or may be upregulated by the presence of a defense-inducing agent. The invention also discloses methods for producing the expression vectors and transgenic plants.

Abstract: The present invention relates to methods for separating cells using immunorosettes. The method involves contacting a sample containing nucleated cells and red blood cells with an antibody composition which allows immunorosettes of the nucleated cells and the red blood cells to form. The antibody composition preferably contains bifunctional antibodies or tetrameric antibody complexes.

Abstract: The present invention relates to methods for separating cells using immunorosettes. The method involves contacting a sample containing nucleated cells and red blood cells with an antibody composition which allows immunorosettes of the nucleated cells and the red blood cells to form. The antibody composition preferably contains bifunctional antibodies or tetrameric antibody complexes.

Abstract: The present invention is directed to a promoter derived from an Arabidopsis ATP sulfurase gene. Nucleic acid constructs, vectors, plant cells and transgenic plants comprising the promoter are also provided.

Abstract: A system for determining a level of a liquid present in a storage container in an automobile is disclosed. The system has a liquid level sensor for determining a preliminary liquid level of the liquid stored in the storage container, an ignition key position sensor for determining an ignition position, a vehicle speed sensor for determining the automobile's speed, a transmission gear selection sensor for determining a transmission gear position of a transmission of the automobile, and a processor. The processor receives input signals from the liquid level sensor, ignition key position sensor, the vehicle speed sensor and the transmission gear selection sensor for calculating the level of the liquid in the storage container.