Patents by Inventor Tetsuo Nagano
Tetsuo Nagano has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20090263910Abstract: A method for measuring hypochlorite ion, which comprises the steps of: (A) reacting, with hypochlorite ion, a compound represented by the following general formula (I): wherein R1 represents a 2-carboxyphenyl group which may be substituted; R2 represents a phenyl group which is substituted with a substituted or unsubstituted amino group; X1 and X2 each independently represents either hydrogen atom or a halogen atom; or a salt thereof; and (B) measuring fluorescence of a dearylated compound generated in the aforementioned step (A) or a salt thereof.Type: ApplicationFiled: June 29, 2009Publication date: October 22, 2009Applicants: DAIICHI PURE CHEMICALS CO., LTDInventors: Ken-Ichi Setsukinai, Yasuteru Urano, Tetsuo Nagano
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Publication number: 20090258434Abstract: A compound represented by the following general formula (I): {R1, R3, R6 and R8 are methyl groups and the like, R2 and R7 are cyano groups and the like, R4 and R5 are fluorine atoms and the like, and X is a group represented by the formula (A) [R11 is a monocarboxy-substituted C1-8 alkoxy group and the like, R12, R14 and R15 are hydrogen atoms and the like, and R13 is hydroxy group and the like], provided that the combination of the aforementioned substituents is such a combination that the compound represented by the formula (I) can be substantially non-fluorescent before reacting with peroxynitrite, and the compound represented by the formula (I) in which the group represented by the formula (A) is nitrated after reacting with peroxynitrite can be substantially highly fluorescent}, or a salt thereof, which is useful as a fluorescent probe for peroxynitrite measurement.Type: ApplicationFiled: November 14, 2006Publication date: October 15, 2009Applicant: THE UNIVERSITY OF TOKYOInventors: Tetsuo Nagano, Yasuteru Urano, Tasuku Ueno
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Publication number: 20090118471Abstract: A compound represented by the following general formula (I) or a salt thereof [R1 represents hydrogen atom, or a C1-12 alkyl group; R2 represents a C1-12 alkyl group having one or more maleimide groups, an aryl group having two or more maleimide groups, a group represented by the formula (A): —(CH2)n—CO—N(R11)(R12) (R11 and R12 represent hydrogen atom, a C1-12 alkyl group, a C1-12 alkyl group having one or more maleimide groups, or an aryl group having one or more maleimide groups, and n represents an integer of 1 to 6), or a group represented by the formula (B): —(CH2)m—NH—CO—R13 (R13 represents a C1-12 alkyl group having one or more maleimide groups, or an aryl group having one or more maleimide groups, and m represents an integer of 1 to 6); R3 and R5 represent hydrogen atom, or a halogen atom; R4 represents hydroxyl group, a C1-12 acyloxy group, a C1-12 acyloxymethyloxy group, or an amino group], which is useful as a novel fluorescent labeling agent having a property of emitting intense fluorescence onlyType: ApplicationFiled: December 17, 2008Publication date: May 7, 2009Applicant: The University of TokyoInventors: Tetsuo NAGANO, Yasuteru Urano, Takuji Shoda
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Patent number: 7524974Abstract: A fluorescent probe which is represented by the following formula (I): (wherein, R1 and R2 represent hydrogen atom, or a substituent for trapping proton, a metal ion, or an active oxygen species, or the like; R3 represents a monovalent substituent; R4 and R5 represent hydrogen atom or a halogen atom; R6 represents hydrogen atom, an alkylcarbonyl group, or an alkylcarbonyloxymethyl group, provided that a combination of R1, R2, and R3 provides (1) substantially high electron density of the benzene ring to which said groups bind, so that the compound represented by the formula (I) is substantially no fluorescent before the trapping of proton, or the like, and (2) substantially reduced electron density of the benzene ring to which said groups bind, so that a compound after the trapping, which is derived from the compound represented by the formula (I) is substantially highly fluorescent after the trapping of proton or the like). A fluorescent probe having an excellent fluorescence property is provided.Type: GrantFiled: July 7, 2003Date of Patent: April 28, 2009Assignees: Daiichi Pure Chemicals Co., Ltd.Inventors: Tetsuo Nagano, Yasuteru Urano, Suguru Kenmoku, Kohjiro Kanda
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Patent number: 7524876Abstract: A compound represented by the following general formula (I) or a salt thereof: [wherein R1 and R2 represent hydrogen atom, a C1-6 alkyl group, or a group represented by the following formula (A): [wherein X1 and X2 represent hydrogen atom, or a group represented as —N(R3)(R4) (R3 and R4 represent hydrogen atom, a C1-6 alkyl group, a C1-6 alkylcarbonyl group, or a C1-6 alkyloxycarbonyl group); and n represents an integer of 1 to 6 ], provided that R1 and R2 do not simultaneously represent hydrogen atom), which is a novel luciferin derivative that serves as a luciferase substrate.Type: GrantFiled: July 27, 2006Date of Patent: April 28, 2009Assignee: The University of TokyoInventors: Hideo Takakura, Yasuteru Urano, Tetsuo Nagano
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Patent number: 7482473Abstract: A compound represented by the following general formula (I) or a salt thereof [R1 represents hydrogen atom, or a C1-12 alkyl group; R2 represents a C1-12 alkyl group having one or more maleimide groups, an aryl group having two or more maleimide groups, a group represented by the formula (A): —(CH2)n—CO—N(R11)(R12) (R11 and R12 represent hydrogen atom, a C1-12 alkyl group, a C1-12 alkyl group having one or more maleimide groups, or an aryl group having one or more maleimide groups, and n represents an integer of 1 to 6), or a group represented by the formula (B): —(CH2)m—NH—CO—R13 (R13 represents a C1-12 alkyl group having one or more maleimide groups, or an aryl group having one or more maleimide groups, and m represents an integer of 1 to 6); R3 and R5 represent hydrogen atom, or a halogen atom; R4 represents hydroxyl group, a C1-12 acyloxy group, a C1-12 acyloxymethyloxy group, or an amino group], which is useful as a novel fluorescent labeling agent having a property of emitting intense fluorescence onlyType: GrantFiled: September 7, 2006Date of Patent: January 27, 2009Assignee: The University of TokyoInventors: Tetsuo Nagano, Yasuteru Urano, Takuji Shoda
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Publication number: 20080281104Abstract: A membrane anchoring-type fluorescent probe consisting of a compound comprising a phospholipid residue, a fluorescent probe compound residue, and a linker which binds the residues (e.g., the compound represented by the following formula).Type: ApplicationFiled: March 3, 2006Publication date: November 13, 2008Applicant: THE UNIVERSITY OF TOKYOInventors: Tetsuo Nagano, Hirotatsu Kojima, Takashi Osaki
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Publication number: 20080261314Abstract: A compound represented by the following general formula (I) or a salt thereof: wherein R1 represents hydrogen atom, an alkyl group, an alkoxy group, or hydroxy group; R2 represents a group represented by the following formula (A): wherein X1 to X4 represent hydrogen atom, an alkyl group, or 2-pyridylmethyl group, and m and n represent 0 or 1; Y represents a single bond or —CO—; R3 represents a carboxy-substituted aryl group, a carboxy-substituted heteroaryl group, benzothiazol-2-yl group, or 5-oxo-2-thioxo-4-imidazolyzinylidenmethyl group], and a fluorescent probe for zinc which comprises said compound or a salt thereof.Type: ApplicationFiled: March 28, 2008Publication date: October 23, 2008Applicants: DAIICHI PURE CHEMICALS CO., LTD., TETSUO NAGANOInventors: Tetsuo NAGANO, Kazuya Kikuchi, Tomoya Hirano, Satoko Maruyama
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Publication number: 20080249321Abstract: A fluorescent probe which is represented by the following formula (wherein R1 and R2 represent hydrogen atom, or a substituent for trapping proton, a metal ion, or an active oxygen species; R3 represents a monovalent substituent other than hydrogen atom, carboxy group, or sulfo group; R4 and R5 represent hydrogen atom, a halogen atom, or an alkyl group; R6 to R9 represent an alkyl group; R10 and R11 represent hydrogen atom, a halogen atom, or an alkyl group; M? represents a counter ion; and the combination of R1, R2, and R3 (1) imparts a substantially high electron density to the benzene ring to which they bond so that the compound can be substantially non-fluorescent before trapping proton, a metal ion, or an active oxygen species, and (2) substantially reduces electron density of the benzene ring to which they bond so that the compound after trapping proton, a metal ion, or an active oxygen species can be highly fluorescent after the trapping.Type: ApplicationFiled: March 3, 2005Publication date: October 9, 2008Applicants: Sekisui Medical Co., Ltd.Inventors: Tetsuo Nagano, Yoko Wada, Yasuteru Urano
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Publication number: 20080188006Abstract: A method for measuring hypochlorite ion, which comprises the steps of. (A) reacting, with hypochlorite ion, a compound represented by the following general formula (I): wherein R1 represents a 2-carboxyphenyl group which may be substituted; R2 represents a phenyl group which is substituted with a substituted or unsubstituted amino group; X1 and X2 each independently represents either hydrogen atom or a halogen atom; or a salt thereof; and (B) measuring fluorescence of a dearylated compound generated in the aforementioned step (A) or a salt thereof.Type: ApplicationFiled: April 9, 2008Publication date: August 7, 2008Applicants: TETSUO NAGANO, DAIICHI PURE CHEMICALS CO., LTD.Inventors: Ken-ichi SETSUKINAI, Yasuteru URANO, Tetsuo NAGANO
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Patent number: 7378282Abstract: A method for measuring hypochlorite ion, which comprises the steps of: (A) reacting, with hypochlorite ion, a compound represented by the following general formula (I): wherein R1 represents a 2-carboxyphenyl group which may be substituted; R2 represents a phenyl group which is substituted with a substituted or unsubstituted amino group; X1 and X2 each independently represents either hydrogen atom or a halogen atom; or a salt thereof; and (B) measuring fluorescence of a dearylated compound generated in the aforementioned step (A) or a salt thereof.Type: GrantFiled: May 14, 2003Date of Patent: May 27, 2008Assignees: Daiichi Pure Chemicals Co., Ltd.Inventors: Ken-ichi Setsukinai, Yasuteru Urano, Tetsuo Nagano
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Publication number: 20080108141Abstract: A compound represented by the following formula (I) or (II): wherein, in the formula (I), R1, R2, R3, and R4 independently represent methyl group or ethyl group; and in the formula (II), R5, R6, R7, and R8 independently represent methyl group or ethyl group and X— represents an anion, and a reagent for measurement of nitric oxide which comprises said compound.Type: ApplicationFiled: June 4, 2007Publication date: May 8, 2008Applicants: DAIICHI PURE CHEMICALS CO., LTD.Inventors: Tetsuo NAGANO, Hirotatsu KOJIMA, Kazuya KIKUCHI
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Publication number: 20080014147Abstract: A tumor cell or tumor tissue-selective fluorescent staining agent comprising a compound represented by the following general formula (I): wherein R1 and R2 independently represent a C1-C4 alkyl group which may be substituted, a C2-C4 alkenyl group which may be substituted, a C2-C4 alkynyl group which may be substituted, an aryl group which may be substituted, or a heteroaryl group which may be substituted.Type: ApplicationFiled: February 23, 2007Publication date: January 17, 2008Applicant: The University of TokyoInventors: Tetsuo Nagano, Yasuteru Urano, Hirotatsu Kojima, Yuuta Fujikawa, Tetsuro Takamatsu, Yoshinori Harada
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Publication number: 20080014602Abstract: A fluorescent probe which is represented by the following formula (I): (wherein, R1 represents a monovalent substituent other than hydrogen atom, carboxy group, or sulfo group; R2 represents hydrogen atom, or a monovalent substituent; R3 and R4 each independently represents hydrogen atom or a halogen atom; and R5 represents a monovalent group which is cleaved by contact with a measuring object, provided that a combination of R1 and R2 is selected so that the oxidation potential of the benzene ring to which they bind makes (1) the compound represented by the formula (I) substantially no fluorescent before the cleavage, and (2) a compound after the cleavage, which is derived from the compound represented by the formula (I), substantially highly fluorescent after the cleavage).Type: ApplicationFiled: September 3, 2004Publication date: January 17, 2008Inventors: Tetsuo Nagano, Mako Kamiya, Yasuteru Urano
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Publication number: 20070298507Abstract: A fluorescent probe which specifically and efficiently traps nitrogen monoxide, zinc ion etc. to emit fluorescence is provided. A compound represented by the following general formula (I): [wherein R1 and R2 represent hydrogen atom, or a group represented by the following formula (A): (wherein X1 to X4 represent hydrogen atom, an alkyl group, or a protective group for amino group, and m and n represent 0 or 1); R3 and R4 represent hydrogen atom, a C1-6 alkyl group, or a C1-6 alkoxy group; R5 to R12 represent hydrogen atom, sulfo group, phospho group, a halogen atom, or a C1-6 alkyl group; R13 and R14 represent a C1-18 alkyl group; Z1 represents oxygen atom, sulfur atom, or —N(R15)— (wherein R15 represents hydrogen atom, or a C1-6 alkyl group); Y1 and Y2 represent —C(?O)—, —C(?S)—, or —C(R16)(R17) (wherein R16 and R17 represent a C1-6 alkyl group); and M? represents a counter ion in a number required for neutralizing the charge].Type: ApplicationFiled: February 22, 2005Publication date: December 27, 2007Inventors: Tetsuo Nagano, Eita Sasaki, Hirotatsu Kojima, Tomoya Hirano, Kazuya Kikuchi
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Patent number: 7297690Abstract: A luminescent lanthanide complex using a photo induced electron transfer as the luminescence controlling principle is presented. The complex comprises a substituted 2-quinolinol containing a sensor group and a complex group and lanthanide ion (Ln3+). This complex is allowed to be co-present in a liquid phase with a material to be measured, and the luminescence of said complex is measured.Type: GrantFiled: February 17, 2004Date of Patent: November 20, 2007Assignee: Japan Science and Technology AgencyInventors: Kazuya Kikuchi, Shinya Iwasawa, Tetsuo Nagano
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Publication number: 20070197778Abstract: A compound represented by the following general formula (I) or a salt thereof [R1 represents hydrogen atom, or a C1-12 alkyl group; R2 represents a C1-12 alkyl group having one or more maleimide groups, an aryl group having two or more maleimide groups, a group represented by the formula (A): —(CH2)n—CO—N(R11)(R12) (R11 and R12 represent hydrogen atom, a C1-12 alkyl group, a C1-12 alkyl group having one or more maleimide groups, or an aryl group having one or more maleimide groups, and n represents an integer of 1 to 6), or a group represented by the formula (B): —(CH2)m—NH—CO—R13 (R13 represents a C1-12 alkyl group having one or more maleimide groups, or an aryl group having one or more maleimide groups, and m represents an integer of 1 to 6); R3 and R5 represent hydrogen atom, or a halogen atom; R4 represents hydroxyl group, a C1-12 acyloxy group, a C1-12 acyloxymethyloxy group, or an amino group], which is useful as a novel fluorescent labeling agent having a property of emitting intense fluorescence onlyType: ApplicationFiled: September 7, 2006Publication date: August 23, 2007Applicant: The University of TokyoInventors: Tetsuo Nagano, Yasuteru Urano, Takuji Shoda
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Publication number: 20070155806Abstract: A compound represented by the following general formula (I) or a salt thereof: [wherein R1 and R2 represent hydrogen atom, a C1-6 alkyl group, or a group represented by the following formula (A): [wherein X1 and X2 represent hydrogen atom, or a group represented as —N(R3)(R4) (R3 and R4 represent hydrogen atom, a C1-6 alkyl group, a C1-6 alkylcarbonyl group, or a C1-6 alkyloxycarbonyl group); and n represents an integer of 1 to 6], provided that R1 and R2 do not simultaneously represent hydrogen atom), which is a novel luciferin derivative that serves as a luciferase substrate.Type: ApplicationFiled: July 27, 2006Publication date: July 5, 2007Applicant: The University of TokyoInventors: Hideo Takakura, Yasuteru Urano, Tetsuo Nagano
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Publication number: 20070117211Abstract: A compound represented by the following formula (I): wherein R1 and R2 represent amino groups that substitute at adjacent positions on the phenyl ring, provided that either of R1 and R2 represents a mono(C1-6 alkyl)-substituted amino group and the other represents an unsubstituted amino group; and R3 and R4 independently represent hydrogen atom or an acyl group, and an agent for measurement of nitrogen monoxide which comprises said compound.Type: ApplicationFiled: May 8, 2006Publication date: May 24, 2007Applicants: Daiichi Pure Chemicals Co., Ltd., Tetsuo NAGANOInventors: Tetsuo Nagano, Hirotatsu Kojima
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Patent number: RE40572Abstract: A compound represented by general formula (IA) or a salt thereof useful as a fluorescent probe for zinc: wherein R1 and R2 represent a hydrogen atom or a group represented by formula (A), wherein X1, X2, X3, and X4 represent a hydrogen atom, an alkyl group, a 2-pyridylmethyl group, or a protective group for an amino group, and m and n represent 0 or 1 provided that R1 and R2 do not simultaneously represent hydrogen atoms; R3 and R4 represent a hydrogen atom or a halogen atom; and R5 and R6 represent a hydrogen atom, an alkylcarbonyl group, or an alkylcarbonyloxymethyl group, and R7 represents a hydrogen atom or an alkyl group.Type: GrantFiled: February 28, 2001Date of Patent: November 11, 2008Assignees: Sekisui Medical Co., Ltd.Inventors: Tetsuo Nagano, Kazuya Kikuchi, Tomoya Hirano