Abstract: A nucleic acid amplification reaction vessel has a substrate, a cavity formed in the substrate, a cover plate for sealing the cavity, and a sample-injection inlet formed in the cover plate. The cavity includes a columnar structure connected to the cover plate or the substrate. The nucleic acid amplification reaction vessel allows fast temperature increase or decrease of sample liquid.

Abstract: The present invention provides a kit employed for polymerase chain reaction. The kit has a cavity and a flow channel on a substrate. The flow channel is separated from the cavity by at least a barrier formed along the cavity. Such a structure allows the cavity to be filled with a sample solution even in minute quantities. At the same time, the structure can provide a sample solution with a rapid temperature-control. The structure can therefore contribute to accelerated polymerase chain reaction.

Abstract: This invention provides an apparatus for determining a biologically specifically reactive substance using the acceleration of a biologically specific reaction by pearl chaining of carrier particles. The apparatus can suppress the generation of heat caused by the pearl chaining to suppress the vaporization of a sample solution. The apparatus comprises a substrate comprising a pair of electrodes and a reaction field disposed between the pair of electrodes, voltage applying means for applying an alternating voltage to the pair of electrodes, and carrier particles which are disposed in the reaction field and undergo pearl chaining upon exposure of an alternating electric field applied to the reaction field by the alternating voltage. The apparatus is characterized in that the voltage applying means applies two or more alternating voltages different from each other in amplitude.

Abstract: A multicomponent analysis sensor for measuring two or more kinds of the subjects to be measured by using redox reactions, which is a multicomponent analysis sensor comprising a liquid sample inlet from which a liquid sample containing two or more kinds of the subjects to be measured is introduced, a first measurement chamber, a second measurement chamber, a first channel connecting the above-described liquid sample inlet to the above-described first measurement chamber and a second channel connecting the above-described first measurement chamber to the second measurement chamber, wherein the above-described first measurement chamber and the above-described second measurement chamber respectively have a working electrode and a counter electrode. A first reagent layer containing an enzyme and an electron transfer substance is provided in the first channel or the first chamber, while another reagent layer containing an enzyme is provided in the second channel or the second chamber.

Abstract: A sample liquid analytical chip that whilst being of simple structure, has a reaction region and a detection region isolated from each other and further has liquid feed control means for a sample liquid, thereby realizing speedy reliable analysis. The sample liquid analytical chip comprises a tubular sample liquid transfer channel with its both ends open, a reaction reagent layer disposed on the internal wall surface of the sample liquid transfer channel and a detection region for detecting any physical or chemical change of the sample liquid. In the sample liquid analytical chip, the sample liquid transfer channel at an area of its wall surface is provided an opening and a lid fitted to the opening. Portion of the lid forms a hinge in cooperation with the wall surface.

Abstract: A sensor of pyrophosphate which can detect pyrophosphate conveniently with high sensitivity in a method for measuring pyrophosphate in SNP typing utilizing a primer extension reaction is provided. A sensor of pyrophosphate which is characterized by including: an insulative substrate 1; an electrode system that is formed thereon and has a measurement electrode 2 and a counter electrode 3; and a plurality of reaction reagent layers that are provided on the substrate 1 and include pyrophosphatase, glyceraldehyde-3-phosphate dehydrogenase, diaphorase, glyceraldehyde-3-phosphate, oxidized nicotineamide adenine dinucleotide, an electronic mediator, a magnesium salt and a buffer component, reaction reagent layer 36 including the enzyme being separated from reaction reagent layer 35 including the buffer component, and reaction reagent layer 37 including glyceraldehyde-3-phosphate being separated from the reaction reagent layer 35 including the buffer component.

Abstract: A fluid chip has a liquid support 25 for supporting a liquid 24, a fluid passage 22 formed by being surrounded by the liquid support 25 and through which the liquid 24 moves, and a plurality of magnetic particles 21 disposed in the fluid passage 24. The magnetic particles 21 are aggregated by a permanent magnet 23 so as to block the fluid passage 22 and thus cut off flow of the liquid 24. The magnetic particles 21 are dispersed along a part of a wall of the fluid passage 22 so as to open the fluid passage 22 and thus allow the liquid 24 to flow.

Abstract: A liquid delivery apparatus 1 has a rotary substrate 2. The substrate 2 formed with a fluid passage 8A for communicating a supply chamber 6A and target chamber 7A. An inlet end portion 13 at which the fluid passage 8A is connected to the chamber 6A extends in a clockwise direction R1 of rotational directions of the substrate 2. The inlet end portion 13 holds a liquid 9 in the chamber 6A by a capillary force. The rotation drive unit 4 rotates the substrate 2 so that an inertial force exceeding the capillary force and directed to the clockwise direction R1 acts on the liquid 9 in the inlet end portion 13. Delivery behavior control of microfluid with a high degree of flexibility can be achieved.

Abstract: The present invention provides a kit employed for polymerase chain reaction. The kit has a cavity and a flow channel on a substrate. The flow channel is separated from the cavity by at least a barrier formed along the cavity. Such a structure allows the cavity to be filled with a sample solution even in minute quantities. At the same time, the structure can provide a sample solution with a rapid temperature-control. The structure can therefore contribute to accelerated polymerase chain reaction.

Abstract: A sensor of pyrophosphate which can detect pyrophosphate conveniently with high sensitivity in a method for measuring pyrophosphate in SNP typing utilizing a primer extension reaction is provided. A sensor of pyrophosphate which is characterized by including: an insulative substrate 1; an electrode system that is formed thereon and has a measurement electrode 2 and a counter electrode 3; and a plurality of reaction reagent layers that are provided on the substrate 1 and include pyrophosphatase, glyceraldehyde-3-phosphate dehydrogenase, diaphorase, glyceraldehyde-3-phosphate, oxidized nicotineamide adenine dinucleotide, an electronic mediator, a magnesium salt and a buffer component, reaction reagent layer 36 including the enzyme being separated from reaction reagent layer 35 including the buffer component, and reaction reagent layer 37 including glyceraldehyde-3-phosphate being separated from the reaction reagent layer 35 including the buffer component.

Abstract: An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3? side of a target SNP base is G, while a base adjacent with one base spaced apart is G. According to the present invention, the 3? end base is designed to be the base corresponding to SNP; the second base from the 3? end to be T or G; the third base from the 3? end to be any one of T or G; and the base sequence of from the fourth from the 3? end to the 5? end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3? side to a desired base.

Abstract: The present invention provides a kit employed for polymerase chain reaction. The kit has a cavity and a flow channel on a substrate. The flow channel is separated from the cavity by at least a barrier formed along the cavity. Such a structure allows the cavity to be filled with a sample solution even in minute quantities. At the same time, the structure can provide a sample solution with a rapid temperature-control. The structure can therefore contribute to accelerated polymerase chain reaction.

Abstract: The present invention provides a blood processing apparatus that can introduce a metered quantity of a blood sample into a chamber and does not require an opening linked to the chamber to be closed after the introduction of the blood sample. An introducing chamber, an injection port, and an air port are formed in a rotatable platform of a blood processing apparatus. The injection port links the introducing chamber with the outside of the rotatable platform. A blood sample is introduced from the injection port into the introducing chamber. A blood coagulant is held on the port wall surface of the air port and/or around a portion of the surface of the rotatable platform where the air port is open.

Abstract: An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3? side of a target SNP base is G, while a base adjacent with one base spaced apart is C. According to the present invention, the 3? end base is designed to be the base corresponding to SNP; the second base from the 3? end to be T or G; the third base from the 3? end to be any one of A, T or C; and the base sequence of from the fourth from the 3? end to the 5? end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3? side to a desired base.

Abstract: An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3? side of a target SNP base is G, while a base adjacent with one base spaced apart is C. According to the present invention, the 3? end base is designed to be the base corresponding to SNP; the second base from the 3? end to be T or G; the third base from the 3? end to be any one of A, T or C; and the base sequence of from the fourth from the 3? end to the 5? end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3? side to a desired base.

Abstract: An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3? side of a target SNP base is G, while a base adjacent with one base spaced apart is G. According to the present invention, the 3? end base is designed to be the base corresponding to SNP; the second base from the 3? end to be T or G; the third base from the 3? end to be any one of T or G; and the base sequence of from the fourth from the 3? end to the 5? end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3? side to a desired base.

Abstract: An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3? side of a target SNP base is G, while a base adjacent with one base spaced apart is T. According to the present invention, the 3? end base is designed to be the base corresponding to SNP; the second base from the 3? end to be T or G; the third base from the 3? end to be G or C; and the base sequence of from the fourth from the 3? end to the 5? end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3? side to a desired base.

Abstract: An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3? side of a target SNP base is A, while a base adjacent with one base spaced apart is G. According to the present invention, the 3? end base is designed to be the base corresponding to SNP; the second base from the 3? end to be C; the third base from the 3? end to be any one of A, T or G; and the base sequence of from the fourth from the 3? end to the 5? end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3? side to a desired base.

Abstract: An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3? side of a target SNP base is G, while a base adjacent with one base spaced apart is C. According to the present invention, the 3? end base is designed to be the base corresponding to SNP; the second base from the 3? end to be T or G; the third base from the 3? end to be any one of A, T or C; and the base sequence of from the fourth from the 3? end to the 5? end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3? side to a desired base.

Abstract: An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3? side of a target SNP base is G, while a base adjacent with one base spaced apart is T. According to the present invention, the 3? end base is designed to be the base corresponding to SNP; the second base from the 3? end to be T or G; the third base from the 3? end to be G or C; and the base sequence of from the fourth from the 3? end to the 5? end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3? side to a desired base.