Abstract: It is an object of the present invention to provide a method for introducing a protein into a plant, which is simple and extensively applicable to various types of plant cells and proteins. The above object is achieved by the present invention to provide a complex comprising a protein of interest to be introduced into a target plant cell and a carrier peptide, a method for introducing a protein of interest into a target plant cell using the complex, and a kit comprising a protein of interest to be introduced into a target plant cell and a carrier peptide.

Abstract: A work vehicle includes an engine, a particulate filter, and an oil separator. The particulate filter is connected to the engine to collect particulate matters contained in exhaust gas from the engine. The particulate filter is provided to overlap with the engine when viewed in a height direction along a height of the work vehicle. The oil separator is connected to the engine via a blow-by-gas discharge pipe to catch a liquid component in blow-by-gas from the engine. The oil separator is provided between the engine and the particulate filter to overlap with the particulate filter when viewed in the height direction.

Abstract: A seat suspension includes a base including a base hook and a seat platform provided with a seat. A first link couples a first rotational axis of the base and a third rotational axis of the seat platform and is configured to be rotatable about the first rotational axis and the third rotational axis. A second link couples a second rotational axis of the base and a fourth rotational axis of the seat platform and is configured to be rotatable about the second rotational axis and the fourth rotational axis. A link hook is provided on the second link. An elastic member is latched to the base hook and the link hook and is configured to pull the link hook toward the base hook. A first angle between a contracting direction of the elastic member and a rotation direction of the link hook increases as the elastic member expands.

Abstract: [Problem to be Solved] It is intended to provide a fusion polypeptide that regulates the transcription of a target gene. [Solution] The present inventors have provided a fusion polypeptide comprising: a cell-penetrating peptide; a DNA-binding polypeptide; and a transcriptional regulator.

Abstract: The present invention provides the following: a vector for inserting a desired nucleic acid into a predetermined site of a nucleic acid comprising a region formed of a first nucleotide sequence, the predetermined site, and a region composed of a second nucleotide sequence, in the stated order in the 5?-to-3? direction, wherein the vector comprises a region formed of the first nucleotide sequence, the desired nucleic acid, and the second nucleotide sequence in the stated order in the 5?-to-3? direction; a kit that includes this vector; a method of inserting a nucleic acid comprising a step for introducing this vector into a cell; a cell acquired by this method; and an organism comprising this cell.

Abstract: The object of the present invention is to, by analyzing PPR proteins that act to bind to DNA with a prediction that RNA recognition rules of PPR motifs can also be used for recognition of DNA, find a PPR protein showing such a characteristic. According to the present invention, it was revealed that, with a protein that can bind in a DNA base-selective manner or a DNA base sequence-specific manner, which contains one or more, preferably 2 to 30, more preferably 5 to 25, most preferably 9 to 15, of PPR motifs having a structure of the following formula 1 (wherein, in the formula 1, Helix A is a part that can form an ?-helix structure; X does not exist, or is a part consisting of 1 to 9 amino acids; Helix B is a part that can form an ?-helix structure; and L is a part consisting of 2 to 7 amino acids), and having a specific combination of amino acids corresponding to a DNA base or DNA base sequence as amino acids of three positions of No. 1 A.A., No. 4 A.A., in Helix A of the formula 1 and No. “ii” (?2) A.A.

Abstract: The object of the present invention is to, by analyzing PPR proteins that act to bind to DNA with a prediction that RNA recognition rules of PPR motifs can also be used for recognition of DNA, find a PPR protein showing such a characteristic. According to the present invention, it was revealed that, with a protein that can bind in a DNA base-selective manner or a DNA base sequence-specific manner, which contains one or more, preferably 2 to 30, more preferably 5 to 25, most preferably 9 to 15, of PPR motifs having a structure of the following formula 1 (wherein, in the formula 1, Helix A is a part that can form an ?-helix structure; X does not exist, or is a part consisting of 1 to 9 amino acids; Helix B is a part that can form an ?-helix structure; and L is a part consisting of 2 to 7 amino acids), and having a specific combination of amino acids corresponding to a DNA base or DNA base sequence as amino acids of three positions of No. 1 A.A., No. 4 A.A., in Helix A of the formula 1 and No. “ii” (?2) A. A.

Abstract: The present invention provides an artificial nuclease comprising a DNA-binding domain and a function domain linked to each other via a polypeptide consisting of 35 to 55 amino acid residues wherein amino acid residues at two sites in a DNA-binding module contained in a DNA-binding domain exhibit a mode of repetition that is different for every four DNA-binding modules; a vector for expressing said artificial nuclease; a vector library for preparing said vector; and a vector set for preparing said vector library.

Abstract: According to this invention, a bird lays an egg that does not contain an artificially introduced foreign gene in genome thereof and has an ovomucoid content lower than that in a wild type thereof. A method for producing a bird includes a modification step of cleaving the ovomucoid gene locus in a pluripotent stem cell of a bird with programmable endonuclease so as to modify the ovomucoid gene locus and a transplantation step of transplanting the pluripotent stem cell in which the ovomucoid gene locus is modified into an embryo of a bird. A bird egg does not contain an artificially introduced foreign gene in genome thereof and has an ovomucoid content lower than that in a wild type thereof.

Abstract: The present invention provides an artificial nuclease comprising a DNA-binding domain and a function domain linked to each other via a polypeptide consisting of 35 to 55 amino acid residues wherein amino acid residues at two sites in a DNA-binding module contained in a DNA-binding domain exhibit a mode of repetition that is different for every four DNA-binding modules; a vector for expressing said artificial nuclease; a vector library for preparing said vector; and a vector set for preparing said vector library.

Abstract: The present invention provides an artificial nuclease comprising a DNA-binding domain and a function domain linked to each other via a polypeptide consisting of 35 to 55 amino acid residues wherein amino acid residues at two sites in a DNA-binding module contained in a DNA-binding domain exhibit a mode of repetition that is different for every four DNA-binding modules; a vector for expressing said artificial nuclease; a vector library for preparing said vector; and a vector set for preparing said vector library.

Abstract: Image forming apparatus, including: image bearer; charging unit for electrically charging image bearer surface; developing unit for developing with toner, electrostatic latent image formed over image bearer by exposure unit for performing light exposure; transfer unit for transferring developed toner to receiving member; and cleaning unit for cleaning toner remaining over image bearer without being transferred, wherein the toner contains: external additives; and base particles made of at least binder resin and colorant, external additive content of the toner is from 4 to 7 parts by mass relative to 100 parts by mass of base particles, primary particles of at least one external additive have a number average particle diameter of 0.01 to 0.05 ?m, cleaning unit includes an elastic-body blade having surface elastic modulus of from 15 to 25 N/mm2 and surface friction coefficient of from 0.5 to 0.7 at an abutment part thereof abutting on image bearer.

Abstract: The present invention provides the following: a vector for inserting a desired nucleic acid into a predetermined site of a nucleic acid comprising a region formed of a first nucleotide sequence, the predetermined site, and a region composed of a second nucleotide sequence, in the stated order in the 5?-to-3? direction, wherein the vector comprises a region formed of the first nucleotide sequence, the desired nucleic acid, and the second nucleotide sequence in the stated order in the 5?-to-3? direction; a kit that includes this vector; a method of inserting a nucleic acid comprising a step for introducing this vector into a cell; a cell acquired by this method; and an organism comprising this cell.

Abstract: The present invention provides an artificial nuclease comprising a DNA-binding domain and a function domain linked to each other via a polypeptide consisting of 35 to 55 amino acid residues wherein amino acid residues at two sites in a DNA-binding module contained in a DNA-binding domain exhibit a mode of repetition that is different for every four DNA-binding modules; a vector for expressing said artificial nuclease; a vector library for preparing said vector; and a vector set for preparing said vector library.

Abstract: An image forming apparatus is provided which includes an image bearer, a charger, an irradiator, a developing device to develop an electrostatic latent image on the image bearer with a toner to form a toner image, a transfer device to transfer the toner image onto a transfer medium, and a cleaner to remove toner particles remaining on the image bearer without being transferred. The toner includes a mother particle including a binder resin and a colorant and one or more external additives. At least one of the external additives includes primary particles having a number average particle diameter in the range of 0.05 to 0.30 ?m. The cleaner includes an elastic body blade having a contact part with the image bearer. The contact part has a surface elastic modulus in the range of 15 to 25 N/mm2 and a surface friction coefficient in the range of 0.5 to 0.7.

Abstract: The present invention provides an artificial nuclease comprising a DNA-binding domain and a function domain linked to each other via a polypeptide consisting of 35 to 55 amino acid residues wherein amino acid residues at two sites in a DNA-binding module contained in a DNA-binding domain exhibit a mode of repetition that is different for every four DNA-binding modules; a vector for expressing said artificial nuclease; a vector library for preparing said vector; and a vector set for preparing said vector library.

Abstract: The object of the present invention is to, by analyzing PPR proteins that act to bind to DNA with a prediction that RNA recognition rules of PPR motifs can also be used for recognition of DNA, find a PPR protein showing such a characteristic. According to the present invention, it was revealed that, with a protein that can bind in a DNA base-selective manner or a DNA base sequence-specific manner, which contains one or more, preferably 2 to 30, more preferably 5 to 25, most preferably 9 to 15, of PPR motifs having a structure of the following formula 1 (wherein, in the formula 1, Helix A is a part that can form an ?-helix structure; X does not exist, or is a part consisting of 1 to 9 amino acids; Helix B is a part that can form an ?-helix structure; and L is a part consisting of 2 to 7 amino acids), and having a specific combination of amino acids corresponding to a DNA base or DNA base sequence as amino acids of three positions of No. 1 A.A., No. 4 A.A., in Helix A of the formula 1 and No. “ii” (-2) A.A.

Abstract: An image forming apparatus is provided which includes an image bearer, a charger, an irradiator, a developing device to develop an electrostatic latent image on the image bearer with a toner to form a toner image, a transfer device to transfer the toner image onto a transfer medium, and a cleaner to remove toner particles remaining on the image bearer without being transferred. The toner includes a mother particle including a binder resin and a colorant and one or more external additives. At least one of the external additives includes primary particles having a number average particle diameter in the range of 0.05 to 0.30 ?m. The cleaner includes an elastic body blade having a contact part with the image bearer. The contact part has a surface elastic modulus in the range of 15 to 25 N/mm2 and a surface friction coefficient in the range of 0.5 to 0.7.

Abstract: An image forming apparatus includes: a rotatable transfer member rotatably driven while carrying a toner image thereon; a rotary transfer member to form a transfer nip for transferring the toner image to a recording medium; a housing rotatable about a first rotation center together with the rotary transfer member and switching between a closed state and an open state by the rotation about the first rotation center; a guide member to guide the recording medium conveyed through a conveyance path toward upstream in the direction of rotation of the rotatable transfer member than the transfer nip; a support frame to support the rotatable transfer member; a biasing member to bias the guide member; and a receiving part, disposed on the support frame, configured to contact the guide member biased by the biasing member and position the guide member in the closed state of the housing.

Abstract: An image forming apparatus, includes: a main body; a cover configured to be provided so as to be capable of being opened and closed with respect to the main body; an attachment unit configured to be provided on the cover; a unit configured to be attached to the attachment unit; a positioning member configured to, on closing of the cover, position the unit with respect to a member attached to the main body or the main body; a guiding member configured to guide the unit to the positioning member; and a protrusion configured to be provided at the guiding member to protrude in an attaching direction of the unit.