Abstract: The present invention relates to: recombinant DNA encoding the SbfI restriction endonuclease as well as the SbfI methylase, and expression of the SbfI restriction endonuclease and SbfI methylase in E. coli cells containing the recombinant DNA; and methods for cloning the SbfI restriction gene (sbfIR) from Streptomyces species Bf-61 into E. coli by PCR. The method relied on primers based on DNA sequences predicted from amino acid sequences of the purified SbfI restriction endonuclease.

Abstract: In accordance with the present invention, there is provided a DNA (deoxyribonucleic acid) fragment which encodes the MmeI type II restriction endonuclease enzyme.

Abstract: An adjustable web cutter which allows web samples to be cut in various lengths, width and thickness while employing a disposable cutter blade.

Abstract: A plastic bag having a venting structure therein including flexible plastic front and rear walls sealed along their side edges and a venting sealing arrangement at one end of the walls including elongated spaced heat-sealed portions having puckers formed therebetween and unsealed openings proximate the sealed portions to permit pressurized gases from within the bag to pass through the unsealed openings and the puckered portions to the atmosphere, the puckered portions normally maintaining the portions of the side walls which form the puckers in contiguous contacting engagement to function as normally closed valves which prevent atmospheric air from entering the bag. The bag may have one or both walls fabricated from polyethylene containing ethyl vinyl acetate which permits the bag to have controlled breathing to permit oxygen to enter the bag at a controlled rate to maintain freshness of the product.