Patents by Inventor Theodore M. Klein
Theodore M. Klein has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230265448Abstract: Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.Type: ApplicationFiled: October 19, 2022Publication date: August 24, 2023Applicants: E. I. DU PONT DE NEMOUS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.Inventors: WILLIAM JAMES GORDON-KAMM, THEODORE M KLEIN, KEITH S LOWE, KEVIN E MCBRIDE, CHRISTOPHER J SCELONGE, NING WANG
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Patent number: 11512321Abstract: Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.Type: GrantFiled: February 22, 2021Date of Patent: November 29, 2022Inventors: William James Gordon-Kamm, Theodore M Klein, Keith S Lowe, Kevin E McBride, Christopher J Scelonge, Ning Wang
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Publication number: 20220124998Abstract: The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS/WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.Type: ApplicationFiled: January 7, 2022Publication date: April 28, 2022Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: AJITH ANAND, MAREN L ARLING, ALEXANDRE DA SILVA CONCEICAO, WILLIAM JAMES GORDON-KAMM, CRAIG HASTINGS, GEORGE J HOERSTER, TODD J JONES, THEODORE M KLEIN, CARLOS M LA ROTA, KEITH S LOWE, SHIV BAHADUR TIWARI, NING WANG, XINLI EMILY WU
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Patent number: 11236347Abstract: Methods and compositions for Ochrobactrum-mediated transformation of plants are provided. Methods include but are not limited to using an Ochrobactrum strain to transfer a polynucleotide of interest to a plant cell. These include VirD2-dependent methods. Compositions include an Ochrobactrum strain, transfer DNAs, constructs and/or plasmids. These include Ochrobactrum strains having a plasmid comprising one or more virulence gene(s), border region, and/or origin of replication. Plant cells, tissues, plants, and seeds comprising a polynucleotide of interest produced by the methods are also provided.Type: GrantFiled: August 26, 2016Date of Patent: February 1, 2022Inventors: Ajith Anand, Steven Henry Bass, Sean M. Bertain, Hyeon-Je Cho, Anthony J. Kinney, Theodore M. Klein, Michael Lassner, Kevin E. McBride, York Moy, Barbara Ann Marie Rosen, Jun-Zhi Wei
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Publication number: 20210277409Abstract: Spectinomycin resistant or streptomycin resistant transgenic plants and methods of making such plants are provided.Type: ApplicationFiled: June 25, 2019Publication date: September 9, 2021Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: AJITH ANAND, HYEON-JE CHO, THEODORE M KLEIN
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Publication number: 20210180077Abstract: Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.Type: ApplicationFiled: February 22, 2021Publication date: June 17, 2021Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E. I. DU PONT DE NEMOURS AND COMPANYInventors: WILLIAM JAMES GORDON-KAMM, THEODORE M KLEIN, KEITH S LOWE, KEVIN E MCBRIDE, CHRISTOPHER J SCELONGE, NING WANG
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Publication number: 20200270622Abstract: Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include tissue-preferred promoters operably linked to morphogenic genes. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence disclosed herein operably linked to a morphogenic gene. DNA constructs comprising a promoter operably linked to a morphogenic gene and further comprising a heterologous nucleotide sequence of interest are also provided.Type: ApplicationFiled: September 19, 2018Publication date: August 27, 2020Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ALEXANDRE DA SILVA CONCEICAO, ALLISON M FINCH, WILLIAM JAMES GORDON-KAMM, THEODORE M KLEIN, KEITH S LOWE
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Publication number: 20180371480Abstract: Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include nucleotide sequences for regulatory regions. Phospholipid transfer protein (PLTP) promoters are provided. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence, such as a PLTP promoter, disclosed herein. DNA constructs comprising a promoter operably linked to a heterologous nucleotide sequence of interest are also provided.Type: ApplicationFiled: August 26, 2016Publication date: December 27, 2018Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ALEXANDRE DA SILVA CONCEICAO, WILLIAM JAMES GORDON-KAMM, THEODORE M. KLEIN, CARLOS M. LA ROTA, KEITH S. LOWE
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Publication number: 20180216123Abstract: Methods and compositions for Ochrobactrum-mediated transformation of plants are provided. Methods include but are not limited to using an Ochrobactrum strain to transfer a polynucleotide of interest to a plant cell. These include VirD2-dependent methods. Compositions include an Ochrobactrum strain, transfer DNAs, constructs and/or plasmids. These include Ochrobactrum strains having a plasmid comprising one or more virulence gene(s), border region, and/or origin of replication. Plant cells, tissues, plants, and seeds comprising a polynucleotide of interest produced by the methods are also provided.Type: ApplicationFiled: August 26, 2016Publication date: August 2, 2018Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E. I. DU PONT DE NEMOURS AND COMPANYInventors: AJITH ANAND, STEVEN HENRY BASS, SEAN M. BERTAIN, HYEON-JE CHO, ANTHONY J. KINNEY, THEODORE M. KLEIN, MICHAEL LASSNER, KEVIN E. MCBRIDE, YORK MOY, BARBARA ANN MARIE ROSEN, JUN-ZHI WEI
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Patent number: 8962328Abstract: Compositions and methods are provided for the efficient transformation of a dicot plant. More particularly, compositions and methods of the present invention find use in agriculture for Agrobacterium-mediated transformation of a dicotyledonous plant. The compositions include cultivation media comprising high concentrations of sucrose and glucose. The cultivation media find use in methods directed to Agrobacterium-mediated transformation of a dicot plant with a gene of interest. In this manner, any gene of interest can be introduced into a dicot plant with high transformation efficiency and reduced tissue necrosis.Type: GrantFiled: April 3, 2012Date of Patent: February 24, 2015Assignees: Pioneer Bred International Inc, E.I. DuPont de NemoursInventors: Hyeon-Je Cho, Linda A. Castle, Theodore M. Klein, Tai Minh, York Moy
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Publication number: 20130055472Abstract: Compositions and methods for the efficient transformation and regeneration of monocot plants are provided. The methods of transformation involve infection with Agrobacterium. In this manner, any gene of interest can be introduced into the monocot plant with high transformation efficiency and in low copy number. Transformed and regenerated monocot cells, tissues, plants, and seed are also provided. The invention encompasses regenerating transformed plants, transgenic seeds produced therefrom, and transgenic plants and transgenic seeds from subsequent generations.Type: ApplicationFiled: August 30, 2012Publication date: February 28, 2013Applicants: E.I. du Pont de Nemours and Company, Pioneer Hi Bred International Inc.Inventors: Myeong-Je Cho, Theodore M. Klein, Zuo-Yu Zhao
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Publication number: 20130019339Abstract: This invention relates to an isolated nucleic acid fragment encoding a transcription factor. The invention also relates to the construction of a chimeric gene encoding all or a portion of the transcription factor, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of accumulated oil in a transformed host cell.Type: ApplicationFiled: September 14, 2012Publication date: January 17, 2013Applicant: E I DU PONT DE NEMOURS AND COMPANYInventors: Rebecca E. Cahoon, Theodore M. Klein
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Publication number: 20120255070Abstract: Compositions and methods are provided for the efficient transformation of a dicot plant. More particularly, compositions and methods of the present invention find use in agriculture for Agrobacterium-mediated transformation of a dicotyledonous plant. The compositions include cultivation media comprising high concentrations of sucrose and glucose. The cultivation media find use in methods directed to Agrobacterium-mediated transformation of a dicot plant with a gene of interest. In this manner, any gene of interest can be introduced into a dicot plant with high transformation efficiency and reduced tissue necrosis.Type: ApplicationFiled: April 3, 2012Publication date: October 4, 2012Applicant: Pioneer Hi Bred International Inc.Inventors: Hyeon-Je Cho, Linda A. Castle, Theodore M. Klein, Tai Minh, York Moy
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Publication number: 20110167516Abstract: Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.Type: ApplicationFiled: December 30, 2010Publication date: July 7, 2011Applicant: Pioneer Hi-Bred International, Inc.Inventors: William J. Gordon-Kamm, Theodore M. Klein, Keith S. Lowe, Kevin E. McBride, Christopher J. Scelonge, Bing-Bing Wang, Ning Wang, Xinli E. Wu
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Publication number: 20110162101Abstract: This invention relates to an isolated nucleic acid fragment encoding a transcription factor. The invention also relates to the construction of a chimeric gene encoding all or a portion of the transcription factor, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of accumulated oil in a transformed host cell.Type: ApplicationFiled: December 16, 2010Publication date: June 30, 2011Applicant: E. I. Du Pont De Nemours and CompanyInventors: Rebecca E. Cahoon, Theodore M. Klein
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Publication number: 20110162111Abstract: Methods of transforming a plant cell with a nucleic acid of interest and regenerating plants therefrom are disclosed. The methods are particularly useful for the transformation of dicot or monocot mature somatic embryos.Type: ApplicationFiled: December 22, 2010Publication date: June 30, 2011Applicant: Pioneer Hi-Bred International, Inc.Inventor: Theodore M. Klein
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Patent number: 7888560Abstract: This invention relates to an isolated nucleic acid fragment encoding a transcription factor. The invention also relates to the construction of a chimeric gene encoding all or a portion of the transcription factor, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of accumulated oil in a transformed host cell.Type: GrantFiled: September 4, 2007Date of Patent: February 15, 2011Assignee: E.I. du Pont de Nemours and CompanyInventors: Rebecca E. Cahoon, Theodore M. Klein
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Patent number: 7825234Abstract: The present invention provides a method of screening for the purpose of obtaining gene expression modulating elements and gene insulator elements. The invention includes a method of identifying gene expression modulating elements and gene insulator elements through use of the following steps: a) locating intergenic regions of a plant genome that are flanked by a gene on each side that have differing gene expressions b) taking that intergenic region or a portion of that intergenic region and adding it to a cassette comprising an isolated gene c) introducing the cassette into a plant cell d) analyzing expression of the isolated gene. The present invention also includes identified sequences that act as gene expression modulating elements.Type: GrantFiled: November 30, 2009Date of Patent: November 2, 2010Inventors: Hochul Yi, Theodore M. Klein, Joanne E. Barton
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Patent number: 7790874Abstract: The present invention provides a method of screening for the purpose of obtaining gene expression modulating elements and gene insulator elements. The invention includes a method of identifying gene expression modulating elements and gene insulator elements through use of the following steps: a) locating intergenic regions of a plant genome that are flanked by a gene on each side that have differing gene expressions b) taking that intergenic region or a portion of that intergenic region and adding it to a cassette comprising an isolated gene c) introducing the cassette into a plant cell d) analyzing expression of the isolated gene. The present invention also includes identified sequences that act as gene expression modulating elements.Type: GrantFiled: November 30, 2009Date of Patent: September 7, 2010Assignee: Pioneer Hi-Bred International, Inc.Inventors: Hochul Yi, Theodore M. Klein, Joanne E. Barton
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Patent number: 7732668Abstract: This invention relates to an isolated nucleic acid fragment encoding floral development proteins, more specifically FT, TFL or Ap3 homologs. The invention also relates to the construction of a recombinant DNA construct encoding all or a portion of the floral development proteins, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the FT, TFL or Ap3 homologs in a transformed host cell.Type: GrantFiled: November 20, 2006Date of Patent: June 8, 2010Assignee: Pioneer Hi-Bred International, Inc.Inventors: Olga Danilevskaya, Pedro Herman, Edward Bruggemann, David M Shirbroun, Evgueni Ananiev, Antoni J Rafalski, Hajime Sakai, Edgar B Cahoon, Rebecca E Cahoon, Theodore M Klein