Patents by Inventor Theodore M. Klein

Theodore M. Klein has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20220124998
    Abstract: The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS/WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.
    Type: Application
    Filed: January 7, 2022
    Publication date: April 28, 2022
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: AJITH ANAND, MAREN L ARLING, ALEXANDRE DA SILVA CONCEICAO, WILLIAM JAMES GORDON-KAMM, CRAIG HASTINGS, GEORGE J HOERSTER, TODD J JONES, THEODORE M KLEIN, CARLOS M LA ROTA, KEITH S LOWE, SHIV BAHADUR TIWARI, NING WANG, XINLI EMILY WU
  • Patent number: 11236347
    Abstract: Methods and compositions for Ochrobactrum-mediated transformation of plants are provided. Methods include but are not limited to using an Ochrobactrum strain to transfer a polynucleotide of interest to a plant cell. These include VirD2-dependent methods. Compositions include an Ochrobactrum strain, transfer DNAs, constructs and/or plasmids. These include Ochrobactrum strains having a plasmid comprising one or more virulence gene(s), border region, and/or origin of replication. Plant cells, tissues, plants, and seeds comprising a polynucleotide of interest produced by the methods are also provided.
    Type: Grant
    Filed: August 26, 2016
    Date of Patent: February 1, 2022
    Inventors: Ajith Anand, Steven Henry Bass, Sean M. Bertain, Hyeon-Je Cho, Anthony J. Kinney, Theodore M. Klein, Michael Lassner, Kevin E. McBride, York Moy, Barbara Ann Marie Rosen, Jun-Zhi Wei
  • Publication number: 20210277409
    Abstract: Spectinomycin resistant or streptomycin resistant transgenic plants and methods of making such plants are provided.
    Type: Application
    Filed: June 25, 2019
    Publication date: September 9, 2021
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: AJITH ANAND, HYEON-JE CHO, THEODORE M KLEIN
  • Publication number: 20210180077
    Abstract: Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.
    Type: Application
    Filed: February 22, 2021
    Publication date: June 17, 2021
    Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E. I. DU PONT DE NEMOURS AND COMPANY
    Inventors: WILLIAM JAMES GORDON-KAMM, THEODORE M KLEIN, KEITH S LOWE, KEVIN E MCBRIDE, CHRISTOPHER J SCELONGE, NING WANG
  • Publication number: 20200270622
    Abstract: Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include tissue-preferred promoters operably linked to morphogenic genes. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence disclosed herein operably linked to a morphogenic gene. DNA constructs comprising a promoter operably linked to a morphogenic gene and further comprising a heterologous nucleotide sequence of interest are also provided.
    Type: Application
    Filed: September 19, 2018
    Publication date: August 27, 2020
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: ALEXANDRE DA SILVA CONCEICAO, ALLISON M FINCH, WILLIAM JAMES GORDON-KAMM, THEODORE M KLEIN, KEITH S LOWE
  • Publication number: 20180371480
    Abstract: Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include nucleotide sequences for regulatory regions. Phospholipid transfer protein (PLTP) promoters are provided. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence, such as a PLTP promoter, disclosed herein. DNA constructs comprising a promoter operably linked to a heterologous nucleotide sequence of interest are also provided.
    Type: Application
    Filed: August 26, 2016
    Publication date: December 27, 2018
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: ALEXANDRE DA SILVA CONCEICAO, WILLIAM JAMES GORDON-KAMM, THEODORE M. KLEIN, CARLOS M. LA ROTA, KEITH S. LOWE
  • Publication number: 20180216123
    Abstract: Methods and compositions for Ochrobactrum-mediated transformation of plants are provided. Methods include but are not limited to using an Ochrobactrum strain to transfer a polynucleotide of interest to a plant cell. These include VirD2-dependent methods. Compositions include an Ochrobactrum strain, transfer DNAs, constructs and/or plasmids. These include Ochrobactrum strains having a plasmid comprising one or more virulence gene(s), border region, and/or origin of replication. Plant cells, tissues, plants, and seeds comprising a polynucleotide of interest produced by the methods are also provided.
    Type: Application
    Filed: August 26, 2016
    Publication date: August 2, 2018
    Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E. I. DU PONT DE NEMOURS AND COMPANY
    Inventors: AJITH ANAND, STEVEN HENRY BASS, SEAN M. BERTAIN, HYEON-JE CHO, ANTHONY J. KINNEY, THEODORE M. KLEIN, MICHAEL LASSNER, KEVIN E. MCBRIDE, YORK MOY, BARBARA ANN MARIE ROSEN, JUN-ZHI WEI
  • Patent number: 8962328
    Abstract: Compositions and methods are provided for the efficient transformation of a dicot plant. More particularly, compositions and methods of the present invention find use in agriculture for Agrobacterium-mediated transformation of a dicotyledonous plant. The compositions include cultivation media comprising high concentrations of sucrose and glucose. The cultivation media find use in methods directed to Agrobacterium-mediated transformation of a dicot plant with a gene of interest. In this manner, any gene of interest can be introduced into a dicot plant with high transformation efficiency and reduced tissue necrosis.
    Type: Grant
    Filed: April 3, 2012
    Date of Patent: February 24, 2015
    Assignees: Pioneer Bred International Inc, E.I. DuPont de Nemours
    Inventors: Hyeon-Je Cho, Linda A. Castle, Theodore M. Klein, Tai Minh, York Moy
  • Publication number: 20130055472
    Abstract: Compositions and methods for the efficient transformation and regeneration of monocot plants are provided. The methods of transformation involve infection with Agrobacterium. In this manner, any gene of interest can be introduced into the monocot plant with high transformation efficiency and in low copy number. Transformed and regenerated monocot cells, tissues, plants, and seed are also provided. The invention encompasses regenerating transformed plants, transgenic seeds produced therefrom, and transgenic plants and transgenic seeds from subsequent generations.
    Type: Application
    Filed: August 30, 2012
    Publication date: February 28, 2013
    Applicants: E.I. du Pont de Nemours and Company, Pioneer Hi Bred International Inc.
    Inventors: Myeong-Je Cho, Theodore M. Klein, Zuo-Yu Zhao
  • Publication number: 20130019339
    Abstract: This invention relates to an isolated nucleic acid fragment encoding a transcription factor. The invention also relates to the construction of a chimeric gene encoding all or a portion of the transcription factor, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of accumulated oil in a transformed host cell.
    Type: Application
    Filed: September 14, 2012
    Publication date: January 17, 2013
    Applicant: E I DU PONT DE NEMOURS AND COMPANY
    Inventors: Rebecca E. Cahoon, Theodore M. Klein
  • Publication number: 20120255070
    Abstract: Compositions and methods are provided for the efficient transformation of a dicot plant. More particularly, compositions and methods of the present invention find use in agriculture for Agrobacterium-mediated transformation of a dicotyledonous plant. The compositions include cultivation media comprising high concentrations of sucrose and glucose. The cultivation media find use in methods directed to Agrobacterium-mediated transformation of a dicot plant with a gene of interest. In this manner, any gene of interest can be introduced into a dicot plant with high transformation efficiency and reduced tissue necrosis.
    Type: Application
    Filed: April 3, 2012
    Publication date: October 4, 2012
    Applicant: Pioneer Hi Bred International Inc.
    Inventors: Hyeon-Je Cho, Linda A. Castle, Theodore M. Klein, Tai Minh, York Moy
  • Publication number: 20110167516
    Abstract: Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.
    Type: Application
    Filed: December 30, 2010
    Publication date: July 7, 2011
    Applicant: Pioneer Hi-Bred International, Inc.
    Inventors: William J. Gordon-Kamm, Theodore M. Klein, Keith S. Lowe, Kevin E. McBride, Christopher J. Scelonge, Bing-Bing Wang, Ning Wang, Xinli E. Wu
  • Publication number: 20110162111
    Abstract: Methods of transforming a plant cell with a nucleic acid of interest and regenerating plants therefrom are disclosed. The methods are particularly useful for the transformation of dicot or monocot mature somatic embryos.
    Type: Application
    Filed: December 22, 2010
    Publication date: June 30, 2011
    Applicant: Pioneer Hi-Bred International, Inc.
    Inventor: Theodore M. Klein
  • Publication number: 20110162101
    Abstract: This invention relates to an isolated nucleic acid fragment encoding a transcription factor. The invention also relates to the construction of a chimeric gene encoding all or a portion of the transcription factor, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of accumulated oil in a transformed host cell.
    Type: Application
    Filed: December 16, 2010
    Publication date: June 30, 2011
    Applicant: E. I. Du Pont De Nemours and Company
    Inventors: Rebecca E. Cahoon, Theodore M. Klein
  • Patent number: 7888560
    Abstract: This invention relates to an isolated nucleic acid fragment encoding a transcription factor. The invention also relates to the construction of a chimeric gene encoding all or a portion of the transcription factor, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of accumulated oil in a transformed host cell.
    Type: Grant
    Filed: September 4, 2007
    Date of Patent: February 15, 2011
    Assignee: E.I. du Pont de Nemours and Company
    Inventors: Rebecca E. Cahoon, Theodore M. Klein
  • Patent number: 7825234
    Abstract: The present invention provides a method of screening for the purpose of obtaining gene expression modulating elements and gene insulator elements. The invention includes a method of identifying gene expression modulating elements and gene insulator elements through use of the following steps: a) locating intergenic regions of a plant genome that are flanked by a gene on each side that have differing gene expressions b) taking that intergenic region or a portion of that intergenic region and adding it to a cassette comprising an isolated gene c) introducing the cassette into a plant cell d) analyzing expression of the isolated gene. The present invention also includes identified sequences that act as gene expression modulating elements.
    Type: Grant
    Filed: November 30, 2009
    Date of Patent: November 2, 2010
    Inventors: Hochul Yi, Theodore M. Klein, Joanne E. Barton
  • Patent number: 7790874
    Abstract: The present invention provides a method of screening for the purpose of obtaining gene expression modulating elements and gene insulator elements. The invention includes a method of identifying gene expression modulating elements and gene insulator elements through use of the following steps: a) locating intergenic regions of a plant genome that are flanked by a gene on each side that have differing gene expressions b) taking that intergenic region or a portion of that intergenic region and adding it to a cassette comprising an isolated gene c) introducing the cassette into a plant cell d) analyzing expression of the isolated gene. The present invention also includes identified sequences that act as gene expression modulating elements.
    Type: Grant
    Filed: November 30, 2009
    Date of Patent: September 7, 2010
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Hochul Yi, Theodore M. Klein, Joanne E. Barton
  • Patent number: 7732668
    Abstract: This invention relates to an isolated nucleic acid fragment encoding floral development proteins, more specifically FT, TFL or Ap3 homologs. The invention also relates to the construction of a recombinant DNA construct encoding all or a portion of the floral development proteins, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the FT, TFL or Ap3 homologs in a transformed host cell.
    Type: Grant
    Filed: November 20, 2006
    Date of Patent: June 8, 2010
    Assignee: Pioneer Hi-Bred International, Inc.
    Inventors: Olga Danilevskaya, Pedro Herman, Edward Bruggemann, David M Shirbroun, Evgueni Ananiev, Antoni J Rafalski, Hajime Sakai, Edgar B Cahoon, Rebecca E Cahoon, Theodore M Klein
  • Publication number: 20100083408
    Abstract: The present invention provides a method of screening for the purpose of obtaining gene expression modulating elements and gene insulator elements. The invention includes a method of identifying gene expression modulating elements and gene insulator elements through use of the following steps: a) locating intergenic regions of a plant genome that are flanked by a gene on each side that have differing gene expressions b) taking that intergenic region or a portion of that intergenic region and adding it to a cassette comprising an isolated gene c) introducing the cassette into a plant cell d) analyzing expression of the isolated gene. The present invention also includes identified sequences that act as gene expression modulating elements.
    Type: Application
    Filed: November 30, 2009
    Publication date: April 1, 2010
    Applicant: E.I. du Pont de Nemours and Company
    Inventors: Hochul Yi, Theodore M. Klein, Joanne E. Barton
  • Publication number: 20100083409
    Abstract: The present invention provides a method of screening for the purpose of obtaining gene expression modulating elements and gene insulator elements. The invention includes a method of identifying gene expression modulating elements and gene insulator elements through use of the following steps: a) locating intergenic regions of a plant genome that are flanked by a gene on each side that have differing gene expressions b) taking that intergenic region or a portion of that intergenic region and adding it to a cassette comprising an isolated gene c) introducing the cassette into a plant cell d) analyzing expression of the isolated gene. The present invention also includes identified sequences that act as gene expression modulating elements.
    Type: Application
    Filed: November 30, 2009
    Publication date: April 1, 2010
    Applicant: E.I. du Pont de Nemours and Company
    Inventors: Hochul Yi, Theodore M. Klein, Joanne E. Barton