Patents by Inventor Thomas A. Tombrello

Thomas A. Tombrello has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11414716
    Abstract: Medical systems for detecting a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a change in signal generated by the fluorophore and quencher, and measured by a sensor of the medical system, when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher, and measured by the sensor of the medical system, when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.
    Type: Grant
    Filed: March 23, 2020
    Date of Patent: August 16, 2022
    Assignee: California Institute of Technology
    Inventors: Aditya Rajagopal, Mark D. Goldberg, Erika F. Garcia, Xiomara L. Madero, Thomas A. Tombrello, Axel Scherer
  • Publication number: 20210002732
    Abstract: Medical systems for detecting a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a change in signal generated by the fluorophore and quencher, and measured by a sensor of the medical system, when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher, and measured by the sensor of the medical system, when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.
    Type: Application
    Filed: March 23, 2020
    Publication date: January 7, 2021
    Inventors: Aditya Rajagopal, Mark D. Goldberg, Erika F. Garcia, Xiomara L. Madero, Thomas A. Tombrello, Axel Scherer
  • Patent number: 10597737
    Abstract: Methods and kits for detecting a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. At least one of the primers is configured to hybridize to a region of the polynucleotide analyte encoding the genetic variation. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a change in signal generated by the fluorophore and quencher when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.
    Type: Grant
    Filed: August 22, 2018
    Date of Patent: March 24, 2020
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Aditya Rajagopal, Mark D. Goldberg, Erika F. Garcia, Xiomara L. Madero, Thomas A. Tombrello, Axel Scherer
  • Publication number: 20190235635
    Abstract: At least one embodiment of this disclosure includes a method for an autonomous vehicle (e.g., a fully autonomous or semi-autonomous vehicle) to communicate with external observers. The method includes: receiving a task at the autonomous vehicle; collecting data that characterizes a surrounding environment of the autonomous vehicle from a sensor coupled to the autonomous vehicle; determining an intended course of action for the autonomous vehicle to undertake based on the task and the collected data; and conveying a human understandable output via an output device, the human understandable output expressly or implicitly indicating the intended course of action to an external observer.
    Type: Application
    Filed: April 10, 2019
    Publication date: August 1, 2019
    Inventors: W. Daniel Hillis, Kjerstin I. Williams, Thomas A. Tombrello, James W. Sarrett, Luke W. Khanlian, Adrian L. Kaehler, Russel Howe
  • Patent number: 10303257
    Abstract: At least one embodiment of this disclosure includes a method for an autonomous vehicle (e.g., a fully autonomous or semi-autonomous vehicle) to communicate with external observers. The method includes: receiving a task at the autonomous vehicle; collecting data that characterizes a surrounding environment of the autonomous vehicle from a sensor coupled to the autonomous vehicle; determining an intended course of action for the autonomous vehicle to undertake based on the task and the collected data; and conveying a human understandable output via an output device, the human understandable output expressly or implicitly indicating the intended course of action to an external observer.
    Type: Grant
    Filed: September 22, 2016
    Date of Patent: May 28, 2019
    Assignee: Applied Invention, LLC
    Inventors: W. Daniel Hillis, Kjerstin I. Williams, Thomas A. Tombrello, James W. Sarrett, Luke W. Khanlian, Adrian L. Kaehler, Russel Howe
  • Publication number: 20190153548
    Abstract: This disclosure provides methods, compositions and kits for the detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, compositions, and kits for detecting analytes, genetic variations, monitoring reaction process, and monitoring analyte-analyte interactions by measuring signals. In some examples, the presence of signals or changes in signals may be used to construct signal profiles which can be used to detect analytes.
    Type: Application
    Filed: August 22, 2018
    Publication date: May 23, 2019
    Inventors: ADITYA RAJAGOPAL, MARK D. GOLDBERG, ERIKA F. GARCIA, XIOMARA L. MADERO, THOMAS A. TOMBRELLO, AXEL SCHERER
  • Patent number: 10081844
    Abstract: Methods of detecting at least one genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, the first primer and the second primer are specific for the polynucleotide analyte. A signal generated by the fluorophore and quencher is measured. PCR is performed with the first primer and the second primer using the polynucleotide analyte as a template, thereby amplifying the template. A signal generated by the fluorophore and quencher from the PCR amplification product is measured. Comparison is made of the signals; and a determination is made of the presence or absence of the at least one genetic variation based i) on the change in signal as determined; and ii) by comparing said change to the change in signal observed upon PCR amplification for a corresponding polynucleotide analyte lacking the at least one genetic variation.
    Type: Grant
    Filed: August 11, 2017
    Date of Patent: September 25, 2018
    Assignee: California Institute of Technology
    Inventors: Aditya Rajagopal, Mark D. Goldberg, Erika F. Garcia, Xiomara L. Madero, Thomas A. Tombrello, Axel Scherer
  • Publication number: 20180030551
    Abstract: This disclosure provides methods, compositions and kits for the detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, compositions, and kits for detecting analytes, genetic variations, monitoring reaction process, and monitoring analyte-analyte interactions by measuring signals. In some examples, the presence of signals or changes in signals may be used to construct signal profiles which can be used to detect analytes.
    Type: Application
    Filed: August 11, 2017
    Publication date: February 1, 2018
    Inventors: Aditya Rajagopal, Mark D. Goldberg, Erika F. Garcia, Xiomara L. Madero, Thomas A. Tombrello, Axel Scherer
  • Patent number: 9846125
    Abstract: Methods and systems for nanopillar sensors are described. Nanopillars can be defined on a substrate, and metal deposited on the nanopillars. A thermal treatment can reflow the metal on the nanopillars forming metallic bulbs on the top end of the nanopillars. These structures can have enhanced optical detection when functionalized with biological agents, or can detect gases, particles and liquids through interaction with the metal layer on the nanopillars.
    Type: Grant
    Filed: February 12, 2015
    Date of Patent: December 19, 2017
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Sameer Walavalkar, Axel Scherer, Thomas A. Tombrello
  • Patent number: 9618477
    Abstract: Methods and devices for sequencing nucleic acids are disclosed herein. Devices are also provided herein for measuring DNA with nano-pores sized to allow DNA to pass through the nano-pore. The capacitance can be measured for the DNA molecule passing through the nano-pore. The capacitance measurements can be correlated to determine the sequence of base pairs passing through the nano-pore to sequence the DNA.
    Type: Grant
    Filed: August 14, 2013
    Date of Patent: April 11, 2017
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Sameer Walavalkar, Axel Scherer, Thomas A. Tombrello, Aditya Rajagopal, Andrew P. Homyk, Erika Garcia
  • Publication number: 20170010679
    Abstract: At least one embodiment of this disclosure includes a method for an autonomous vehicle (e.g., a fully autonomous or semi-autonomous vehicle) to communicate with external observers. The method includes: receiving a task at the autonomous vehicle; collecting data that characterizes a surrounding environment of the autonomous vehicle from a sensor coupled to the autonomous vehicle; determining an intended course of action for the autonomous vehicle to undertake based on the task and the collected data; and conveying a human understandable output via an output device, the human understandable output expressly or implicitly indicating the intended course of action to an external observer.
    Type: Application
    Filed: September 22, 2016
    Publication date: January 12, 2017
    Inventors: W. Daniel HILLIS, Kjerstin I. Williams, Thomas A. Tombrello, James W. Sarrett, Luke W. Khanlian, Adrian L. Kaehler, Russel Howe
  • Patent number: 9475422
    Abstract: At least one embodiment of this disclosure includes a method for an autonomous vehicle (e.g., a fully autonomous or semi-autonomous vehicle) to communicate with external observers. The method includes: receiving a task at the autonomous vehicle; collecting data that characterizes a surrounding environment of the autonomous vehicle from a sensor coupled to the autonomous vehicle; determining an intended course of action for the autonomous vehicle to undertake based on the task and the collected data; and conveying a human understandable output via an output device, the human understandable output expressly or implicitly indicating the intended course of action to an external observer.
    Type: Grant
    Filed: May 22, 2014
    Date of Patent: October 25, 2016
    Assignee: Applied Invention, LLC
    Inventors: W. Daniel Hillis, Kjerstin I. Williams, Thomas A. Tombrello, James W. Sarrett, Luke W. Khanlian, Adrian L. Kaehler, Russel Howe
  • Publication number: 20160280537
    Abstract: Methods for fabricating of high aspect ratio probes and deforming micropillars and nanopillars are described. Use of polymers in deforming nanopillars and micropillars is also described.
    Type: Application
    Filed: June 9, 2016
    Publication date: September 29, 2016
    Inventors: Michael D. HENRY, Andrew P. HOMYK, Axel SCHERER, Thomas A. TOMBRELLO, Sameer WALAVALKAR
  • Patent number: 9390936
    Abstract: Methods for fabricating of high aspect ratio probes and deforming micropillars and nanopillars are described. Use of polymers in deforming nanopillars and micropillars is also described.
    Type: Grant
    Filed: October 29, 2014
    Date of Patent: July 12, 2016
    Inventors: Michael D. Henry, Andrew P. Homyk, Axel Scherer, Thomas A. Tombrello, Sameer Walavalkar
  • Publication number: 20160069810
    Abstract: Methods and systems for nanopillar sensors are described. Nanopillars can be defined on a substrate, and metal deposited on the nanopillars. A thermal treatment can reflow the metal on the nanopillars forming metallic bulbs on the top end of the nanopillars. These structures can have enhanced optical detection when functionalized with biological agents, or can detect gases, particles and liquids through interaction with the metal layer on the nanopillars.
    Type: Application
    Filed: February 12, 2015
    Publication date: March 10, 2016
    Inventors: Sameer WALAVALKAR, Axel SCHERER, Thomas A. TOMBRELLO
  • Patent number: 9243277
    Abstract: The basic structure and functionality of a probe as disclosed herein allows for flexibly incorporating into the probe, various sensing elements for various sensing applications. Two example applications among these various sensing applications include bio-sensing and chemical-sensing applications. For bio-sensing applications the probe, which is fabricated upon a silicon substrate, includes a bio-sensing element such as a nano-pillar transistor, and for chemical-sensing applications the probe includes a sensing element that has a functionalized contact area whereby the sensing element generates a voltage when exposed to one or more chemicals of interest.
    Type: Grant
    Filed: June 29, 2015
    Date of Patent: January 26, 2016
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Aditya Rajagopal, Axel Scherer, Michael D. Henry, Sameer Walavalkar, Thomas A. Tombrello, Andrew P. Homyk
  • Publication number: 20150336502
    Abstract: At least one embodiment of this disclosure includes a method for an autonomous vehicle (e.g., a fully autonomous or semi-autonomous vehicle) to communicate with external observers. The method includes: receiving a task at the autonomous vehicle; collecting data that characterizes a surrounding environment of the autonomous vehicle from a sensor coupled to the autonomous vehicle; determining an intended course of action for the autonomous vehicle to undertake based on the task and the collected data; and conveying a human understandable output via an output device, the human understandable output expressly or implicitly indicating the intended course of action to an external observer.
    Type: Application
    Filed: May 22, 2014
    Publication date: November 26, 2015
    Inventors: W. Daniel HILLIS, Kjerstin I. WILLIAMS, Thomas A. TOMBRELLO, James W. SARRETT, Luke W. KHANLIAN, Adrian L. KAEHLER, Russel HOWE
  • Publication number: 20150299757
    Abstract: The basic structure and functionality of a probe as disclosed herein allows for flexibly incorporating into the probe, various sensing elements for various sensing applications. Two example applications among these various sensing applications include bio-sensing and chemical-sensing applications. For bio-sensing applications the probe, which is fabricated upon a silicon substrate, includes a bio-sensing element such as a nano-pillar transistor, and for chemical-sensing applications the probe includes a sensing element that has a functionalized contact area whereby the sensing element generates a voltage when exposed to one or more chemicals of interest.
    Type: Application
    Filed: June 29, 2015
    Publication date: October 22, 2015
    Inventors: Aditya RAJAGOPAL, Axel SCHERER, Michael D. HENRY, Sameer WALAVALKAR, Thomas A. TOMBRELLO, Andrew P. HOMYK
  • Publication number: 20150283513
    Abstract: Methods and devices for isolating and sorting nanoparticles are disclosed herein. Nanopores of a desired size can be formed in silicon dioxide membranes and used as filters to separate nanoparticles. Devices are also provided herein for sorting nanoparticles with multiple filters having various sized nanopores.
    Type: Application
    Filed: June 19, 2015
    Publication date: October 8, 2015
    Inventors: SAMEER WALAVALKAR, ADITYA RAJAGOPAL, AXEL SCHERER, THOMAS A. TOMBRELLO
  • Patent number: 9099436
    Abstract: The basic structure and functionality of a probe as disclosed herein allows for flexibly incorporating into the probe, various sensing elements for various sensing applications. Two example applications among these various sensing applications include bio-sensing and chemical-sensing applications. For bio-sensing applications the probe, which is fabricated upon a silicon substrate, includes a bio-sensing element such as a nano-pillar transistor, and for chemical-sensing applications the probe includes a sensing element that has a functionalized contact area whereby the sensing element generates a voltage when exposed to one or more chemicals of interest.
    Type: Grant
    Filed: March 28, 2013
    Date of Patent: August 4, 2015
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Aditya Rajagopal, Axel Scherer, Michael D. Henry, Sameer Walavalkar, Thomas A. Tombrello, Andrew P. Homyk